FAM48A (SUPT20H) (NM_001014286) Human Untagged Clone

SKU SC301920

SUPT20H (untagged)-Human family with sequence similarity 48, member A (FAM48A), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: FAM48A
• Synonyms: C13; C13orf19; FAM48A; FP757; P38IP; SPT20
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001014286 edited; GTCGGAAGTGCGGCCGCCGGAACTGGCTGTTGGTGGGGGTGCAGCACTGAGCCGCCGACG; GGGCGGGTGGGCTTTGCTGCCGAGCAGGCGGCGCCGTCTTGGGGCCTAGCGGCGAGGCGA; CCCGCACAGTACTGTAAGATTGATGTTAAAGGCATGGTGTTCACCCCACTTCATCAGCGT; ACATAAGTTATCTCTTCTTTTGGACCCTTATTTTATGCCATAATGCAACAAGCTTTAGAA; CTAGCTTTGGATCGTGCAGAGTATGTCATTGAAAGTGCCCGACAGAGACCTCCTAAAAGG; AAATACCTATCAAGTGGAAGAAAATCTGTATTTCAAAAACTTTATGACTTGTATATTGAA; GAATGTGAAAAAGAACCTGAAGTTAAGAAATTAAGAAGAAATGTGAACTTGTTAGAGAAG; CTTGTTATGCAAGAGACTTTGTCATGTTTAGTGGTCAATCTATACCCAGGAAATGAGGGA; TATTCTCTGATGCTCAGGGGAAAAAACGGATCAGATTCCGAGACCATTCGACTGCCCTAT; GAAGAAGGAGAGTTGCTTGAATATTTGGATGCAGAAGAATTACCTCCTATTTTGGTTGAT; CTCCTAGAAAAATCTCAGGTTAATATTTTTCATTGCGGATGTGTCATAGCAGAAATACGT; GACTACAGGCAGTCCAGTAACATGAAATCTCCTGGTTACCAAAGTCGGCACATTCTCTTA; CGTCCAACAATGCAGACTTTAATTTGTGATGTACATTCAATAACAAGTGATAACCACAAA; TGGACCCAGGAAGACAAACTTTTGCTTGAGAGCCAGCTCATCCTAGCTACAGCTGAACCA; CTCTGTCTTGATCCTTCTATAGCAGTCACCTGCACTGCAAACAGACTGCTCTATAACAAG; CAAAAGATGAACACTCGCCCAATGAAACGGTGTTTCAAGAGGTATTCCAGATCCTCTCTG; AATCGGCAGCAAGATCTATCTCATTGTCCACCTCCTCCTCAGCTGAGGTTACTTGATTTC; TTACAAAAAAGAAAGGAAAGAAAAGCAGGTCAGCATTATGACCTCAAAATTTCTAAGGCA; GGAAATTGTGTAGATATGTGGAAACGGAGTCCCTGTAATTTGGCCATACCTTCTGAAGTA; GATGTGGAGAAATATGCTAAAGTGGAAAAGTCTATCAAATCTGATGACTCACAGCCAACA; GTCTGGCCAGCCCATGATGTAAAAGATGATTATGTATTTGAATGTGAAGCTGGTACTCAG; TATCAGAAAACAAAGCTGACCATCTTGCAGTCGCTTGGAGATCCACTTTACTATGGTAAA; ATACAGCCATGTAAAGCAGATGAAGAAAGTGACAGCCAGATGTCTCCATCACACTCGTCC; ACAGATGATCATTCAAATTGGTTCATTATTGGATCAAAGACCGATGCTGAGAGGGTAGTC; AATCAGTACCAAGAATTAGTCCAGAATGAAGCCAAATGTCCGGTCAAGATGTCACACAGC; TCCAGTGGCTCAGCCAGTCTGAGTCAGGTTTCTCCAGGGAAAGAAACAGATCAAACTGAA; ACCGTGTCAGTTCAGTCTTCGGTATTGGGGAAGGGTGTAAAACATCGACCCCCACCAATC; AAACTTCCCTCAAGCTCAGGAAATAGTTCCTCAGGTAACTATTTTACACCACAACAGACA; AGCAGCTTTCTCAAATCTCCAACTCCTCCTCCTTCTTCTAAGCCATCAAGTATTCCTCGG; AAATCATCTGTGGATCTCAATCAAGTTAGCATGCTTTCTCCAGCTGCCCTATCACCTGCC; AGCTCATCACAAAGAACCACGGCCACCCAGGTCATGGCAAACTCTGCTGGACTTAACTTC; ATCAATGTAGTGGGCTCTGTTTGTGGGGCCCAGGCTTTGATGAGTGGTTCAAACCCCATG; CTGGGCTGTAACACTGGTGCCATAACTCCTGCAGGAATAAACCTGAGCGGCCTTCTACCC; TCAGGAGGTCTGCTACCAAATGCACTGCCCAGTGCAATGCAGGCAGCTTCTCAAGCAGGT; GTTCCATTTGGTTTAAAAAATACTTCAAGTCTCAGGCCCTTAAATCTACTCCAGCTTCCA; GGTGGTTCACTTATTTTTAACACTCTGCAGCAGCAGCAACAGCAGCTCTCCCAGTTTACA; CCACAACAACCTCAGCAGCCCACAACTTGTAGTCCTCAACAGCCAGGGGAGCAGGGTTCT; GAGCAAGGTTCAACCAGTCAAGAACAGGCCTTATCTGCTCAGCAAGCTGCTGTTATTAAC; CTTACTGGAGTAGGAAGTTTTATGCAGTCACAGGCAGCTGTGTTGTCTCAGCTTGGCTCT; GTCGAGAACAGACCTGAGCAAAGCCTTCCTCAGCAGAGATTCCAGCTCTCCTCTGCCTTT; CAACAGCAGCAGCAACAGATACAACAGTTGCGATTCTTGCAGCATCAAATGGCTATGGCA; GCAGCAGCAGCACAAACAGCTCAGCTACATCATCATCGGCATACAGGCAGCCAGTCAAAA; AGTAAAATGAAGAGAGGCACGCCAACCACTCCAAAATTTTGAGTCTTGCATTACTTTTTG; TTCCTTTTTTAAAAACACAAGAGCACTGAATCAAAAGAATTGAGTTTCTACTTTTTGTTT; TTTTTAATGTGTCAGTATTTTACATTGCTAGATGTACAAACTTTATACAGAAGCACAACC; TTATCATTTTTAAATAAAAACAGGG;
• Restriction Sites: Please inquire
• ACCN: NM_001014286
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001014286.1.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001014286.1, NP_001014308.1
• RefSeq Size: 2755 bp
• RefSeq ORF: 2340 bp
• Locus ID: 55578
• UniProt ID: Q8NEM7
• Cytogenetics: 13q13.3
• Protein Families: Transcription Factors
• Summary: Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by FGF signaling and Snail (By similarity). Required for starvation-induced ATG9A trafficking during autophagy.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) differs in the 5' UTR, has an alternate splice site in the 5' coding region and an additional exon in the 3' coding region which results in a frameshift, compared to variant 2. The resulting isoform (a) is longer; it lacks an internal aa in the N-terminal region, but has a longer and distinct C-terminus, compared to isoform b.