GCN2 (EIF2AK4) (NM_001013703) Human Untagged Clone

SKU SC301871

EIF2AK4 (untagged)-Human eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GCN2
• Synonyms: GCN2; PVOD2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001013703 edited; GGCGCAGCGCTGCCATGGCTGGGGGCCGTGGGGCCCCCGGGCGCGGCCGGGACGAGCCTC; CGGAGAGCTACCCGCAACGACAGGACCACGAGCTACAGGCCCTGGAGGCCATCTACGGCG; CGGACTTCCAAGACCTGCGGCCGGACGCTTGCGGACCGGTCAAAGAGCCCCCTGAAATCA; ATTTAGTTTTGTACCCTCAAGGCCTAACTGGTGAAGAAGTATATGTAAAAGTGGATTTGA; GGGTTAAATGCCCACCTACCTATCCAGATGTAGTTCCTGAAATAGAGTTAAAAAATGCCA; AAGGTCTATCAAATGAAAGTGTCAATTTGTTAAAATCTCGCCTAGAAGAACTGGCCAAGA; AACACTGTGGGGAGGTGATGATCTTTGAACTGGCTTACCACGTGCAGTCATTTCTCAGCG; AGCATAACAAGCCCCCTCCCAAGTCTTTTCATGAAGAAATGCTGGAAAGGCGGGCTCAGG; AGGAGCAGCAGAGGCTGTTGGAGGCCAAGCGGAAAGAAGAGCAGGAGCAACGTGAAATCC; TGCATGAGATTCAGAGAAGGAAAGAAGAGATAAAAGAAGAGAAAAAAAGGAAAGAAATGG; CTAAGCAGGAACGTTTGGAAATTGCTAGTTTGTCAAACCAAGATCATACCTCTAAGAAGG; ACCCAGGAGGACACAGAACGGCTGCCATTCTACATGGAGGCTCTCCTGACTTTGTAGGAA; ATGGTAAACATCGGGCAAACTCCTCAGGAAGGTCTAGGCGAGAACGTCAGTATTCTGTAT; GTAATAGTGAAGATTCTCCTGGCTCTTGTGAAATTCTGTATTTCAATATGGGGAGTCCTG; ATCAGCTCATGGTGCACAAAGGGAAATGTATTGGCAGTGATGAACAACTTGGAAAATTAG; TCTACAATGCTTTGGAAACAGCCACTGGTGGCTTTGTCTTGTTGTATGAGTGGGTCCTTC; AGTGGCAGAAAAAAATGGGTCCATTCCTTACCAGTCAAGAAAAAGAGAAGATTGATAAGT; GCAAAAAGCAGATTCAAGGAACAGAAACAGAATTCAACTCACTGGTAAAATTGAGCCATC; CAAATGTAGTACGCTACCTTGCAATGAATCTCAAAGAGCAAGACGACTCCATCGTGGTGG; ACATTTTAGTGGAGCACATTAGTGGGGTCTCTCTTGCTGCACACCTGAGCCACTCAGGCC; CCATCCCTGTGCATCAGCTTCGCAGGTACACAGCTCAGCTCCTGTCAGGCCTTGATTATC; TGCACAGCAATTCTGTGGTGCATAAGGTCCTGAGTGCATCTAATGTCTTGGTGGATGCAG; AAGGCACCGTCAAGATTACGGACTATAGCATTTCTAAGCGCCTCGCAGACATTTGCAAGG; AGGATGTGTTTGAGCAAACCCGAGTTCGTTTTAGTGACAATGCTCTGCCTTATAAAACGG; GGAAGAAAGGAGATGTTTGGCGTCTTGGCCTTCTGCTGCTGTCCCTCAGCCAAGGACAGG; AATGTGGAGAGTACCCTGTGACCATCCCTAGTGACTTACCAGCTGACTTTCAAGATTTTC; TAAAGAAATGTGTGTGCTTGGATGACAAGGAAAGATGGAGTCCCCAGCAGTTGTTGAAAC; ACAGCTTTATAAATCCCCAGCCAAAAATGCCTCTAGTGGAACAAAGTCCTGAAGATTCTG; AAGGACAAGATTATGTTGAGACTGTTATTCCTAGCAACCGGCTACCCAGTGCTGCCTTCT; TTAGTGAGACACAGAGACAGTTTTCCCGATACTTCATTGAGTTTGAAGAATTACAACTTC; TTGGTAAAGGAGCTTTTGGAGCTGTCATCAAGGTGCAGAACAAGTTGGACGGCTGCTGCT; ACGCAGTGAAGCGCATCCCCATCAACCCGGCCAGCCGGCAGTTCCGCAGGATCAAGGGCG; AAGTGACACTGCTGTCACGGCTGCACCATGAGAACATTGTGCGCTACTACAACGCCTGGA; TCGAGCGGCACGAGCGGCCGGCGGGACCGGGGACGCCGCCCCCGGACTCCGGGCCCCTGG; CCAAGGATGACCGAGCTGCACGCGGGCAGCCGGCGAGCGACACAGACGGCCTGGACAGCG; TAGAGGCCGCCGCGCCGCCACCCATCCTCAGCAGCTCGGTGGAGTGGAGCACTTCGGGCG; AGCGCTCGGCCAGTGCCCGTTTCCCCGCCACCGGCCCGGGCTCCAGCGATGACGAGGACG; ACGACGAGGACGAGCACGGTGGCGTCTTCTCCCAGTCCTTCCTGCCTGCTTCAGATTCTG; AAAGTGATATTATCTTTGACAATGAAGATGAGAACAGTAAAAGTCAGAATCAGGATGAAG; ATTGCAATGAAAAGAATGGCTGCCATGAAAGTGAGCCATCAGTGACGACTGAGGCTGTGC; ACTACCTATACATCCAGATGGAGTACTGTGAGAAGAGCACTTTACGAGACACCATTGACC; AGGGACTGTATCGAGACACCGTCAGACTCTGGAGGCTTTTTCGAGAGATTCTGGATGGAT; TAGCTTATATCCATGAGAAAGGAATGATTCACCGGGATTTGAAGCCTGTCAACATTTTTT; TGGATTCTGATGACCATGTGAAAATAGGTGATTTTGGTTTGGCGACAGACCATCTAGCCT; TTTCTGCTGACAGCAAACAAGACGATCAGACAGGAGACTTGATTAAGTCAGACCCTTCAG; GTCACTTAACTGGGATGGTTGGCACTGCTCTCTATGTAAGCCCAGAGGTCCAAGGAAGCA; CCAAATCTGCATACAACCAGAAAGTGGATCTCTTCAGCCTGGGAATTATCTTCTTTGAGA; TGTCCTATCACCCCATGGTCACGGCTTCAGAAAGGATCTTTGTTCTCAACCAACTCAGAG; ATCCCACTTCGCCTAAGTTTCCAGAAGACTTTGACGATGGAGAGCATGCAAAGCAGAAAT; CAGTCATCTCCTGGCTGTTGAACCACGATCCAGCAAAACGGCCCACAGCCACAGAACTGC; TCAAGAGTGAGCTGCTGCCCCCACCCCAGATGGAGGAGTCAGAGCTGCATGAAGTGCTGC; ACCACACGCTGACCAACGTGGATGGGAAGGCCTACCGCACCATGATGGCCCAGATCTTCT; CGCAGCGCATCTCCCCTGCCATCGATTACACCTATGACAGCGACATACTGAAGGGCAACT; TCTCAATCCGTACAGCCAAGATGCAGCAGCATGTGTGTGAAACCATCATCCGCATCTTTA; AAAGACATGGAGCTGTTCAGTTGTGTACTCCACTACTGCTTCCCCGAAACAGACAAATAT; ATGAGCACAACGAAGCTGCCCTATTCATGGACCACAGCGGGATGCTGGTGATGCTTCCTT; TTGACCTGCGGATCCCTTTTGCAAGATATGTGGCAAGAAATAATATATTGAATTTAAAAC; GATACTGCATAGAACGTGTGTTCAGGCCGCGCAAGTTAGATCGATTTCATCCCAAAGAAC; TTCTGGAGTGTGCATTTGATATTGTCACTTCTACCACCAACAGCTTTCTGCCCACTGCTG; AAATTATCTACACTATCTATGAAATCATCCAAGAGTTTCCAGCACTTCAGGAAAGAAATT; ACAGTATTTATTTGAACCATACCATGTTATTGAAAGCAATACTCTTACACTGTGGGATCC; CAGAAGATAAACTCAGTCAAGTCTACATTATTCTGTATGATGCTGTGACAGAGAAGCTGA; CGAGGAGAGAAGTGGAAGCTAAATTTTGTAATCTGTCTTTGTCTTCTAATAGTCTGTGTC; GACTCTACAAGTTTATTGAACAGAAGGGAGATTTGCAAGATCTTATGCCAACAATAAATT; CATTAATAAAACAGAAAACAGGTATTGCACAGTTGGTGAAGTATGGCTTAAAAGACCTAG; AGGAGGTTGTTGGACTGTTGAAGAAACTCGGCATCAAGTTACAGGTCTTGATCAATTTGG; GCTTGGTTTACAAGGTGCAGCAGCACAATGGAATCATCTTCCAGTTTGTGGCTTTCATCA; AACGAAGGCAAAGGGCTGTACCTGAAATCCTCGCAGCTGGAGGCAGATATGACCTGCTGA; TTCCCCAGTTTAGAGGGCCACAAGCTCTGGGGCCAGTTCCCACTGCCATTGGGGTCAGCA; TAGCTATAGACAAGATATCTGCTGCTGTCCTCAACATGGAGGAATCTGTTACAATAAGCT; CTTGTGACCTCCTGGTTGTAAGTGTTGGCCAGATGTCTATGTCCAGGGCCATCAACCTAA; CCCAGAAACTCTGGACAGCAGGCATCACAGCAGAAATCATGTACGACTGGTCACAGTCCC; AAGAGGAATTACAAGAGTACTGCAGACATCATGAAATCACCTATGTGGCCCTTGTCTCGG; ATAAAGAAGGAAGCCATGTCAAGGTTAAGTCTTTCGAGAAGGAAAGGCAGACAGAGAAGC; GTGTGCTGGAGACTGAACTTGTGGACCATGTACTGCAGAAACTGAGGACTAAAGTCACTG; ATGAAAGGAATGGCAGAGAAGCTTCCGATAATCTTGCAGTGCAAAATCTGAAGGGGTCAT; TTTCTAATGCTTCAGGTTTGTTTGAAATCCATGGAGCAACAGTGGTTCCCATTGTGAGTG; TGCTAGCCCCGGAGAAGCTGTCAGCCAGCACTAGGAGGCGCTATGAAACTCAGGTACAAA; CTCGACTTCAGACCTCCCTTGCCAACTTACATCAGAAAAGCAGTGAAATTGAAATTCTGG; CTGTGGATCTACCCAAAGAAACAATATTACAGTTTTTATCATTAGAGTGGGATGCTGATG; AACAGGCATTTAACACAACTGTGAAGCAGCTGCTGTCACGCCTGCCAAAGCAAAGATACC; TCAAATTAGTCTGTGATGAAATTTATAACATCAAAGTAGAAAAAAAGGTGTCTGTGCTAT; TTCTGTACAGCTATAGAGATGACTACTACAGAATCTTATTTTAACCCTGAAGAACTGTCA; TTAACCTCATTCAAACAGACAGAGGCTTATACTGGAATAATGGAATGTTGTACATTCATC; ATAATTTAAAATTAAATTCTAAGAAGAGGCTGGGTGCAGTGGCTCACACCTTTAATCCCA; GCACTTTGGGAAGCCAAGGCAGGAAGACTGCTTGAAACCAGGAGTTTGAGACCAGCCTGA; GCAACAAAGCAAGACCCCATCTCTATAAAAACTAAAAAAATTAGTTGGGCATGGTGGCAC; ATGCCTGTAGTCCCAGCTACTCCAGAGGCTGAGATGGATCATCTGAGCCTCAGGAGGTTG; AGGCTGCAGTGAGCTGTGACTGCGCCACTGCACTCCAGTCTGGGACAACAGAGCAAGACC; CTGTCTTAAAAAAAAAAAGAAAAAAAAATTTTTTTTCTAAGAAGCTGTCCTACAAAGTTG; AGCTTTGTTAGTTTTTCATGTGTAATATATTATAAATTTATCTTTTGGGATATAATAAAT; GCTTTCATATAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001013703 unedited; AGTTCNCATTTGTATACCACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGCAGCGCT; GCCATGGCTGGGGGCCGTGGGGCCCCCGGGCGCGGCCGGGACGAGCCTCCGGAGAGCTAC; CCGCAACGACAGGACCACGAGCTACAGGCCCTGGAGGCCATCTACGGCGCGGACTTCCAA; GACCTGCGGCCGGACGCTTGCGGACCGGTCAAAGAGCCCCCTGAAATCAATTTAGTTTTG; TACCCTCAAGGCCTAACTGGTGAAGAAGTATATGTAAAAGTGGATTTGAGGGTTAAATGC; CCACCTACCTATCCAGATGTAGTTCCTGAAATAGAGTTAAAAAATGCCAAAGGTCTATCA; AATGAAAGTGTCAATTTGTTAAAATCTCGCCTAGAAGAACTGGCCAAGAAACACTGTGGG; GAGGTGATGATCTTTGAACTGGCTTACCACGTGCAGTCATTTCTCAGCGAGCATAACAAG; CCCCCTCCCAAGTCTTTTCATGAAGAAATGCTGGAAAGGCGGGCTCAGGAGGAGCAGCAG; AGGCTGTTGGAGGCCAAGCGGANAGAAGAGCAGGAGCAACGTGAAATCCTGCATGAGATT; CAGAGAAGGAAAGAAGAAGATAAAGAAGAGAANAAAAGGAAGAAATGGCTAAGCAAGAAC; GTTTGAAAATTGCTAGTTTGTCAAACCAAGATCATACCTCTAAGAAGGACCCAGNAGGAC; ACAGAACGGCTGCCAATCTACATGGAAGCTCTCCTGACCTTGTAGGAAATGGTAAACATC; GGGGCANAACTCTCAGGAAAGTCCTAGCCGAGAACGTCAGTAATCTGTAATGTAAAGTGA; AAGATNCTCCTGCCTCTTGGGAAAATCCTGTTTTTAATATGGGGAGTCCCTGATTAGCTC; ATGGGTGCCCAAAGGGAAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001013703 unedited; NCCCCATTGGANGATGGCACTTCCCAGGNCCAGGNANGAGCACTGGNGGNAGGGGTCACA; GGGNATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTC; GAGTTTTTTTTTTTTTTTTTTATATGAAAGCATTTATTATATCCCAAAAGATAAATTTAT; AATATATTACACATGAAAAACTAACAAAGCTCAACTTTGTAGGACAGCTTCTTAGAAAAA; AAATTTTTTTTTCTTTTTTTTTTTAAGACAGGGTCTTGCTCTGTTGTCCCAGACTGGAGT; GCAGTGGCGCAGTCACAGCTCACTGCAGCCTCAACCTCCTGAGGCTCAGATGATCCATCT; CAGCCTCTGGAGTAGCTGGGACTACAGGCATGTGCCACCATGCCCAACTAATTTTTTTAG; TTTTTATAGAGATGGGGTCTTGCTTTGTTGCTCAGGCTGGTCTCAAACTCCTGGTTTCAA; GCAGTCTTCCTGCCTTGGCTTCCCAAAGTGCTGGGATTAAAGGTGTGAGCCACTGCACCC; AGCCTCTTCTTAGAATTTAATTTTAAATTATGATGAATGTACAACATTCCATTATTCCAG; TATAAGCCTCTGTCTGTTTGAATGAGGTTAATGACAGTTCTTCAGGGTTAAAATAAGATT; CTGTAGTAGTCATCTCTATAGCTGTACAGAAATAGCACAGACACCTTTTTTTCTACTTTG; ATGTTATAAATTTCATCACAGACTAATTTGAGGTATCTTTGCTTTGGCAGGCGTGACAGC; AGCTGCTTCACAGTTGTGTTAAATGCCTGTTCATCAGCATCCCACTCTAATGATAAAAAC; TGTTATATTGGTTTCTTTGGGGTAGATCCACAGCCAGAATTTCAAATTCACTGCTTTTCT; GAAGTAAGTTTGCAAAGA;
• Restriction Sites: Please inquire
• ACCN: NM_001013703
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001013703.2, NP_001013725.2
• RefSeq Size: 5532 bp
• RefSeq ORF: 4950 bp
• Locus ID: 440275
• UniProt ID: Q9P2K8
• Cytogenetics: 15q15.1
• Protein Families: Druggable Genome, Protein Kinase
• Summary: This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014]