Plakophilin 1 (PKP1) (NM_001005337) Human Untagged Clone

CAT#: SC300910

PKP1 (untagged)-Human plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1), transcript variant 1a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Plakophilin 1
• Synonyms: B6P; EDSFS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC300910 representing NM_001005337.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAACCACTCGCCGCTCAAGACCGCCTTGGCGTACGAATGCTTCCAGGACCAGGACAACTCCACGTTG
  GCTTTGCCGTCGGACCAAAAGATGAAAACAGGCACGTCTGGCAGGCAGCGCGTGCAGGAGCAGGTGATG
  ATGACCGTCAAGCGGCAGAAGTCCAAGTCTTCCCAGTCGTCCACCCTGAGCCACTCCAATCGAGGTTCC
  ATGTATGATGGCTTGGCTGACAATTACAACTATGGGACCACCAGCAGGAGCAGCTACTACTCCAAGTTC
  CAGGCAGGGAATGGCTCATGGGGATATCCGATCTACAATGGAACCCTCAAGCGGGAGCCTGACAACAGG
  CGCTTCAGCTCCTACAGCCAGATGGAGAACTGGAGCCGGCACTACCCCCGGGGCAGCTGTAACACCACC
  GGCGCAGGCAGCGACATCTGCTTCATGCAGAAAATCAAGGCGAGCCGCAGTGAGCCCGACCTCTACTGT
  GACCCACGGGGCACCCTGCGCAAGGGCACGCTGGGCAGCAAGGGCCAGAAGACCACCCAGAACCGCTAC
  AGCTTTTACAGCACCTGCAGTGGTCAGAAGGCCATAAAGAAGTGCCCTGTGCGCCCGCCCTCTTGTGCC
  TCCAAGCAGGACCCTGTGTATATCCCGCCCATCTCCTGCAACAAGGACCTGTCCTTTGGCCACTCTAGG
  GCCAGCTCCAAGATCTGCAGTGAGGACATCGAGTGCAGTGGGCTGACCATCCCCAAGGCTGTGCAGTAC
  CTGAGCTCCCAGGATGAGAAGTACCAGGCCATTGGGGCCTATTACATCCAGCATACCTGCTTCCAGGAT
  GAATCTGCCAAGCAACAGGTCTATCAGCTGGGAGGCATCTGCAAGCTGGTGGACCTCCTCCGCAGCCCC
  AACCAGAACGTCCAGCAGGCCGCGGCAGGGGCCCTGCGCAACCTGGTGTTCAGGAGCACCACCAACAAG
  CTGGAGACCCGGAGGCAGAATGGGATCCGCGAGGCAGTCAGCCTCCTGAGGAGAACCGGGAACGCCGAG
  ATCCAGAAGCAGCTGACTGGGCTGCTCTGGAACCTGTCTTCCACTGACGAGCTGAAGGAGGAACTCATT
  GCCGACGCCCTGCCTGTTCTGGCCGACCGCGTCATCATTCCCTTCTCTGGCTGGTGCGATGGCAATAGC
  AACATGTCCCGGGAAGTGGTGGACCCTGAGGTCTTCTTCAATGCCACAGGCTGCTTGAGGAACCTGAGC
  TCGGCCGATGCAGGCCGCCAGACCATGCGTAACTACTCAGGGCTCATTGATTCCCTCATGGCCTATGTC
  CAGAACTGTGTAGCGGCCAGCCGCTGTGACGACAAGTCTGTGGAAAACTGCATGTGTGTTCTGCACAAC
  CTCTCCTACCGCCTGGACGCCGAGGTGCCCACCCGCTACCGCCAGCTGGAGTATAACGCCCGCAACGCC
  TACACCGAGAAGTCCTCCACTGGCTGCTTCAGCAACAAGAGCGACAAGATGATGAACAACAACTATGAC
  TGCCCCCTGCCTGAGGAAGAGACCAACCCCAAGGGCAGCGGCTGGTTGTACCATTCAGATGCCATCCGC
  ACCTACCTGAACCTCATGGGCAAGAGCAAGAAAGATGCTACCCTGGAGGCCTGTGCTGGTGCCCTGCAG
  AACCTGACAGCCAGCAAGGGGCTGATGTCCAGTGGCATGAGCCAGTTGATTGGGCTGAAGGAAAAGGGC
  CTGCCACAAATTGCCCGCCTCCTGCAATCTGGCAACTCTGATGTGGTGCGGTCCGGAGCCTCCCTCCTG
  AGCAACATGTCCCGCCACCCTCTGCTGCACAGAGTGATGGGGAACCAGGTGTTCCCGGAGGTGACCAGG
  CTCCTCACCAGCCACACTGGCAATACCAGCAACTCCGAAGACATCTTGTCCTCGGCCTGCTACACTGTG
  AGGAACCTGATGGCCTCGCAGCCACAACTGGCCAAGCAGTACTTCTCCAGCAGCATGCTCAACAACATC
  ATCAACCTGTGCCGAAGCAGTGCCTCACCCAAGGCCGCAGAAGCTGCCCGGCTTCTCCTGTCTGACATG
  TGGTCCAGCAAGGAACTGCAGGGTGTCCTCAGACAGCAAGGTTTCGATAGGAACATGCTGGGAACCTTA
  GCTGGGGCCAACAGCCTCAGGAACTTCACCTCCCGATTCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001005337
• Insert Size: 2181 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005337.2
• RefSeq Size: 5384 bp
• RefSeq ORF: 2181 bp
• Locus ID: 5317
• UniProt ID: Q13835
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome
• MW: 80.5 kDa
• Gene Summary: This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1a) represents the shorter transcript variant as compared to variant 1b. It encodes the shorter protein (isoform 1a), compared to isoform 1b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.