NT5C3 (NT5C3A) (NM_001002009) Human Untagged Clone

SKU SC300387

NT5C3 (untagged)-Human 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: NT5C3
• Synonyms: cN-III; hUMP1; NT5C3; P5'N-1; P5N-1; p36; PN-I; POMP; PSN1; UMPH; UMPH1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001002009 edited; ATGACTAATCAAGAGTCTGCCGTACATGTGAAAATGATGCCAGAATTCCAGAAAAGTTCA; GTTCGAATCAAGAACCCTACAAGAGTAGAAGAAATTATCTGTGGTCTTATCAAAGGAGGA; GCTGCCAAACTTCAGATAATAACGGACTTTGATATGACACTCAGTAGATTTTCATATAAA; GGGAAAAGATGCCCAACATGTCATAATATCATTGACAACTGTAAGCTGGTTACAGATGAA; TGTAGAAAAAAGTTATTGCAACTAAAGGAAAAATATTACGCTATTGAAGTTGATCCTGTT; CTTACTGTAGAAGAGAAGTACCCTTATATGGTGGAATGGTATACTAAATCACATGGTTTG; CTTGTTCAGCAAGCTTTACCAAAAGCTAAACTTAAAGAAATTGTGGCAGAATCTGACGTT; ATGCTCAAAGAAGGATATGAGAATTTCTTTGATAAGCTCCAACAACATAGCATCCCCGTG; TTCATATTTTCGGCTGGAATCGGCGATGTACTAGAGGAAGTTATTCGTCAAGCTGGTGTT; TATCATCCCAATGTCAAAGTTGTGTCCAATTTTATGGATTTTGATGAAACTGGGGTGCTC; AAAGGATTTAAAGGAGAACTAATTCATGTATTTAACAAACATGATGGTGCCTTGAGGAAT; ACAGAATATTTCAATCAACTAAAAGACAATAGTAACATAATTCTTCTGGGAGACTCCCAA; GGAGACTTAAGAATGGCAGATGGAGTGGCCAATGTTGAGCACATTCTGAAAATTGGATAT; CTAAATGATAGAGTGGATGAGCTTTTAGAAAAGTACATGGACTCTTATGATATTGTTTTA; GTACAAGATGAATCATTAGAAGTAGCCAACTCTATTTTACAGAAGATTCTATAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001002009 unedited; TGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCTTCCGAGACTCGCAG; TCTACGCGAGCTGCCTGTTTTTTTCCTGCTTGGACGCGCATGAGGGCCCCGTCCATGGAC; CGCGCGGCCGTGGCGAGGGTGGGCGCGGTAGCGAGCGCCAGCGTGTGCGCCCTGGTGGCG; GGGGTGGTGCTGGCTCAGTACATATTCACCTTGAAGAGGAAGACGGGGCGGAAGACCAAG; ATCATCGAGATGAAGATTGGATAACCAAGAAATGACTAATCAAGAGTCTGCCGTACATGT; GAAAATGATGCCAGAATTCCAGAAAAGTTCAGTTCGAATCAAGAACCCTACAAGAGTAGA; AGAAATTATCTGTGGTCTTATCAAAGGAGGAGCTGCCAAACTTCAGATAATAACGGACTT; TGATATGACACTCAGTAGATTTTCATATAAAGGGAAAAGATGCCCAACATGTCATAATAT; CATTGACAACTGTAAGCTGGTTACAGATGAATGTAGAAAAAAGTTATTGCAACTAAAGGA; AAAATATTACGCTATTGAAGTTGATCCTGTTCTTACTGTAGAAGAGAAGTACCCTTATAT; GGTGGAATGGTATACTAAATCACATGGTTTGGCTTGTTCAGCAAGCTTTACCAAAAGCTA; AACTTAAAGAAATTGTGGCAGAATNCTGACGTTATGCTCAAAGAAGGGATATGAGAATTT; CTTTGATAAGCTNCAACACATAGCATTCCCCGTGTTCATATTTTTCGGCTGGATCGGGCG; ATGTCTANNAGGAGTNATTCGTCAAGCCTGGTGTTATCATCCATGTCCCAAAGGTGGGTC; AATTTTTAGTGATTTTGATGAACAAGGGGGTGCTCAAAGAATTTAAGGAGACTAATTNTT; GTATTTACAACATGAAGGGG;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001002009 unedited; TTACTAATACAGGTTTGCTTGTTCANCAAGCTTTACCAAAAGCTAAACTTAAAGAATTGT; GGCAGAATCTGACGTTATGCTCAAAGAAGGAATGAGAATTTCTTTGATAAGCTCCAACAA; CATAGCATCCCCGTGTTCATATTTTCGGCTGGAATCGGCGATGTACTAGAGGAAGTTATT; CGTCAAGCTGGTGTTTATCATCCCAATGTCAAAGTTGTGTCCAATTTTATGGATTTTGAT; GAAACTGGGGTGCTCAAAGGATTTAAAGGAGAACTAATTCATGTATTTAACAAACATGAT; GGTGCCTTGAGGAATACAGAATATTTCAATCAACTAAAAGACAATAGTAACATAATTCTT; CTGGGAGACTCCCAAGGAGACTTAAGAATGGCAGATGGAGTGGCCAATGTTGAGCACATT; CTGAAAATTGGATATCTAAATGATAGAGTGGATGAGCTTTTAGAAAAGTACATGGACTCT; TATGATATTGTTTTAGTACAAGATGAATCATTAGAAGTAGCCAACTCTATTTTACAGAAG; ATTCTATAAACAAGCATTCTCCAAGAAGACCTCTCTCCTGTGGGTGCAATTGAACTGTTC; ATCCGTTCATCTTGCTGAGAGACTTATTTATAATATATCCTTACTCTCGAAGTGTTCCCT; TTGTATAACTGAAGTATTTTCAGATATGGTGAATGCATTGACTGGAAGCTCCTTTTCTCC; ACCTCTCTCAACACACTCCTTACCGTATCTTTTTAACCATTTAAAAAAAAAAAAGCTANA; ATTAGAAAAATAACTCCCTACTTTTTCCAAGTGAAATTTTGTAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001002009
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001002009.1, NP_001002009.1
• RefSeq Size: 1743 bp
• RefSeq ORF: 894 bp
• Locus ID: 51251
• UniProt ID: Q9H0P0
• Cytogenetics: 7p14.3
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
• Summary: This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4. [provided by RefSeq, Mar 2012]; Transcript Variant: This variant (2) includes an alternate internal exon in the 5' region and initiates translation at an alternate start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which is shorter and has a distinct N-terminus, compared to isoform 1.