CD36 (NM_001001548) Human Untagged Clone

CAT#: SC300210

CD36 (untagged)-Human CD36 molecule (thrombospondin receptor) (CD36), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD36
• Synonyms: BDPLT10; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001001548 edited
  GCAGTGTAGGACTTTCCTGCAGAATACCATTTGATCCTATTAAGAATTGTCCAAATGTTG
  GAGCATTTGATTGAAAAATCCTTCTTAGCCATTTTAAAGATAGCTTTCCAATGATTAGAC
  GAATTGATTCTTTCTGTGACTCATCAGTTCCTTTCCTGTAAAATTCATGTCTTGCTGTTG
  ATTTGTGAATAAGAACCAGAGCTTGTAGAAACCACTTTAATCATATCCAGGAGTTTGCAA
  GAAACAGGTGCTTAACACTAATTCACCTCCTGAACAAGAAAAATGGGCTGTGACCGGAAC
  TGTGGGCTCATCGCTGGGGCTGTCATTGGTGCTGTCCTGGCTGTGTTTGGAGGTATTCTA
  ATGCCAGTTGGAGACCTGCTTATCCAGAAGACAATTAAAAAGCAAGTTGTCCTCGAAGAA
  GGTACAATTGCTTTTAAAAATTGGGTTAAAACAGGCACAGAAGTTTACAGACAGTTTTGG
  ATCTTTGATGTGCAAAATCCACAGGAAGTGATGATGAACAGCAGCAACATTCAAGTTAAG
  CAAAGAGGTCCTTATACGTACAGAGTTCGTTTTCTAGCCAAGGAAAATGTAACCCAGGAC
  GCTGAGGACAACACAGTCTCTTTCCTGCAGCCCAATGGTGCCATCTTCGAACCTTCACTA
  TCAGTTGGAACAGAGGCTGACAACTTCACAGTTCTCAATCTGGCTGTGGCAGCTGCATCC
  CATATCTATCAAAATCAATTTGTTCAAATGATCCTCAATTCACTTATTAACAAGTCAAAA
  TCTTCTATGTTCCAAGTCAGAACTTTGAGAGAACTGTTATGGGGCTATAGGGATCCATTT
  TTGAGTTTGGTTCCGTACCCTGTTACTACCACAGTTGGTCTGTTTTATCCTTACAACAAT
  ACTGCAGATGGAGTTTATAAAGTTTTCAATGGAAAAGATAACATAAGTAAAGTTGCCATA
  ATCGACACATATAAAGGTAAAAGGAATCTGTCCTATTGGGAAAGTCACTGCGACATGATT
  AATGGTACAGATGCAGCCTCATTTCCACCTTTTGTTGAGAAAAGCCAGGTATTGCAGTTC
  TTTTCTTCTGATATTTGCAGGTCAATCTATGCTGTATTTGAATCCGACGTTAATCTGAAA
  GGAATCCCTGTGTATAGATTTGTTCTTCCATCCAAGGCCTTTGCCTCTCCAGTTGAAAAC
  CCAGACAACTATTGTTTCTGCACAGAAAAAATTATCTCAAAAAATTGTACATCATATGGT
  GTGCTAGACATCAGCAAATGCAAAGAAGGGAGACCTGTGTACATTTCACTTCCTCATTTT
  CTGTATGCAAGTCCTGATGTTTCAGAACCTATTGATGGATTAAACCCAAATGAAGAAGAA
  CATAGGACATACTTGGATATTGAACCTATAACTGGATTCACTTTACAATTTGCAAAACGG
  CTGCAGGTCAACCTATTGGTCAAGCCATCAGAAAAAATTCAAGTATTAAAGAATCTGAAG
  AGGAACTATATTGTGCCTATTCTTTGGCTTAATGAGACTGGGACCATTGGTGATGAGAAG
  GCAAACATGTTCAGAAGTCAAGTAACTGGAAAAATAAACCTCCTTGGCCTGATAGAAATG
  ATCTTACTCAGTGTTGGTGTGGTGATGTTTGTTGCTTTTATGATTTCATATTGTGCATGC
  AGATCGAAAACAATAAAATAAACCTGGCTCAAGCACAAACCAATTTGTGTTGTTCTGATT
  CAATAATTGGTTTCTGGGTGGCCAATTCAGAAGAAGAGTGTACATGCTCAACAAATCCTA
  GGCCCTGCATTCCTGTCATCCTCATCCGGGGGAAACACCATCATCCCAGTAGCTGCCCTA
  TTCAACTGCAACAGTCTCCAGGACCATCAGTATACTGCATTTCATGTGCACCAAATATTT
  TGAAAGACATTTATAAATAATTGGCTTATGACTCATATTTCTCTATGAATACCTTCATAC
  AGCAGGTATAACTCTTTTCTTTATGGGCTTAAATATTTTGTCACTGATCCTGCAAATGGA
  CATCATTTTAGCACACTAGCGGTTTATATTTTAAGGACCTTCATTCTCTGTTCTGCACCT
  CTTCTGGAAATTGAGTAAATTTTGCTTTTTTTTTTTTACTCAGTTGCAACTTACGCTTGG
  CATCTTCAGAATGCTTTTCTAGCATTAAGAGATGTAAATGATAAAGGAATTATTGTATGA
  AATATTACAAAGCGTAGACTATGCATTGTTATTCATTATAATATTTTTTCTGTCATAATC
  GCCTCATAAAGACAGGTTTCAACCATTAAAATATGTTCTTCCTTAAAAAAAAAAAAAAAA
  A
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001001548 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGACTTTCCTGCAGAAT
  ACCATTTGATCCTATTAAGAATTGTCCAAATGTTGGAGCATTTGATTGAAAAATCCTTCT
  TAGCCATTTTAAAGATAGCTTTCCAATGATTAGACGAATTGATTCTTTCTGTGACTCATC
  AGTTCCTTTCCTGTAAAATTCATGTCTTGCTGTTGATTTGTGAATAAGAACCAGAGCTTG
  TAGAAACCACTTTAATCATATCCAGGAGTTTGCAAGAAACAGGTGCTTAACACTAATTCA
  CCTCCTGAACAAGAAAAATGGGCTGTGACCGGAACTGTGGGCTCATCGCTGGGGCTGTCA
  TTGGTGCTGTCCTGGCTGTGTTTGGAGGTATTCTAATGCCAGTTGGAGACCTGCTTATCC
  AGAAGACAATTAAAAAGCAAGTTGTCCTCGAAGAAGGTACAATTGCTTTTAAAAATTGGG
  TTAAAACAGGCACAGAAGTTTACAGACAGTTTTGGATCTTTGATGTGCAAAATCCACAGG
  AAGTGATGATGAACAGCAGCAACATTCAAGTTAAGCAAAGAGGTCCTTATACGTACAGAG
  TTCGTTTTCTAGCCAAGGAAAATGTAACCCAGGACGCTGAGGACAACACAGTCTCTTTCC
  TGCAGCCCAATGGTGCCATCTTCGAACCTTCACTATCAGTTGGAACAGAGGCTGACAACT
  TCACAGTTCTCAATCTGGCTGTGGCAGCTGCATCCCATATCTATCAAAATCAATTTGTTC
  AAATGATCCCTCATTCACTTATTAACAAGTCANAATCTTCTATGTTNCCAAGTCAGAACT
  TTGAGAGAAACTGTATGGGGCTATAGGGATCCATTTTTGAGTTTGGTTTCGTACCCTGNT
  ACTACCACAGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001001548 unedited
  TGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAGGAAGAACATA
  TTTTAATGGTTGAAACCTGTCTTTATGAGGCGATTATGACAGCAAAAATATTATAATGAA
  TAACAATGCATAGTCTACGCTTTGTAATATTTCATACAATAATTCCTTTATCATTTACAT
  CTCTTAATGCTAGAAAAGCATTCTGAAGATGCCAAGCGTAAGTTGCAACTGAGTAAAAAA
  AAAAAAGCAAAATTTACTCAATTTCCAGAAGAGGTGCAGAACAGAGAATGAAGGTCCTTA
  AAATATAAACCGCTAGTGTGCTAAAATGATGTCCATTTGCAGGATCAGTGACAAAATATT
  TAAGCCCATAAAGAAAAGAGTTATACCTGCTGTATGAAGGTATTCATAGAGAAATATGAG
  TCATAAGCCAATTATTTATAAATGTCTTTCAAAATATTTGGTGCACATGAAATGCAGTAT
  ACTGATGGTCCTGGAGACTGTTGCAGTTGAATAGGGCAGCTACTGGGATGATGGTGTTTC
  CCCCGGATGAGGATGACAGGAATGCAGGGCCTAGGATTTGTTGAGCATGTACACTCTTCT
  TCTGAATTGGCCACCCAGACACCAATTATTGAATCAGAACACACANATTGGTTTGTGCTT
  GAGCCAGGNTTATTNTATTGTTTTCGATCTGCATGCACCATATGAAATCATAANAAGCAC
  AAACATCACCACACCAACACTGAGTAAGATCATTTCTATCAGGCCAAGGAGGGTTATTTT
  TCCCAGTACTTGACTTCTGAACATGTTTGCCTTCTCATCACCATGGTCCCAGTCTCATTA
  AGCCAAGAATAGCCACATATAGNTCCTCTTCAGATCTTTATACTTGAATTTTTTCTGATG
  GGCTGACCATAGGTGACCTGCAGCCGTTTGCAAATGTGAAGTGATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001001548
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001548.1, NP_001001548.1
• RefSeq Size: 2338 bp
• RefSeq ORF: 1419 bp
• Locus ID: 948
• UniProt ID: P16671
• Cytogenetics: 7q21.11
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, ECM-receptor interaction, Hematopoietic cell lineage, PPAR signaling pathway
• Gene Summary: The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) has an alternate 3' UTR, compared to variant 3. Variants 1 through 5 all encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.