Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (NM_000744) Human Untagged Clone

SKU SC300129

CHRNA4 (untagged)-Human cholinergic receptor, nicotinic, alpha 4 (CHRNA4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Nicotinic Acetylcholine Receptor alpha 4
• Synonyms: BFNC; EBN; EBN1; NACHR; NACHRA4; NACRA4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000744 edited; ATCTAGAGCCCGCGAGGTGCGTGCGCCATGGAGCTAGGGGGCCCCGGAGCGCCGCGGCTG; CTGCCGCCGCTGCTGCTGCTTCTGGGGACCGGCCTCCTGCGCGCCAGCAGCCATGTGGAG; ACCCGGGCCCACGCCGAGGAGCGGCTCCTGAAGAAACTCTTCTCCGGTTACAACAAGTGG; TCCCGACCCGTGGCCAACATCTCGGACGTGGTCCTCGTCCGCTTCGGCCTGTCCATCGCT; CAGCTCATTGACGTGGATGAGAAGAACCAGATGATGACCACGAACGTATGGGTGAAGCAG; GAGTGGCACGACTACAAGCTGCGCTGGGACCCAGCTGACTATGAGAATGTCACCTCCATC; CGCATCCCCTCCGAGCTCATCTGGCGGCCGGACATCGTCCTCTACAACAATGCTGACGGG; GACTTCGCGGTCACCCACCTGACCAAGGCCCACCTGTTCCATGACGGGCGGGTGCAGTGG; ACTCCCCCGGCCATTTACAAGAGCTCCTGCAGCATCGACGTCACCTTCTTCCCCTTCGAC; CAGCAGAACTGCACCATGAAATTCGGCTCCTGGACCTACGACAAGGCCAAGATCGACCTG; GTGAACATGCACAGCCGCGTGGACCAGCTGGACTTCTGGGAGAGTGGCGAGTGGGTCATC; GTGGACGCCGTGGGCACCTACAACACCAGGAAGTACGAGTGCTGCGCCGAGATCTACCCG; GACATCACCTATGCCTTCGTCATCCGGCGGCTGCCGCTCTTCTACACCATCAACCTCATC; ATCCCCTGCCTGCTCATCTCCTGCCTCACCGTGCTGGTCTTCTACCTGCCCTCCGAGTGT; GGCGAGAAGATCACGCTGTGCATCTCCGTGCTGCTGTCGCTCACCGTCTTCCTGCTGCTC; ATCACCGAGATCATCCCGTCCACCTCACTGGTCATCCCACTCATCGGCGAGTACCTGCTG; TTCACCATGATCTTCGTCACCCTGTCCATCGTCATCACGGTCTTCGTGCTCAACGTGCAC; CACCGCTCGCCACGCACGCACACCATGCCCACCTGGGTACGCAGGGTCTTCCTGGACATC; GTGCCACGCCTGCTCCTCATGAAGCGGCCGTCCGTGGTCAAGGACAATTGCCGGCGGCTC; ATCGAGTCCATGCATAAGATGGCCAGTGCCCCGCGCTTCTGGCCCGAGCCAGAAGGGGAG; CCCCCTGCCACGAGCGGCACCCAGAGCCTGCACCCTCCCTCACCGTCCTTCTGCGTCCCC; CTGGATGTGCCGGCTGAGCCTGGGCCTTCCTGCAAGTCACCCTCCGACCAGCTCCCTCCT; CAGCAGCCCCTGGAAGCTGAGAAAGCCAGCCCCCACCCCTCGCCTGGACCCTGCCGCCCG; CCCCACGGCACCCAGGCACCAGGGCTGGCCAAAGCCAGGTCCCTCAGCGTCCAGCACATG; TCCAGCCCTGGCGAAGCGGTGGAAGGCGGCGTCCGGTGCCGGTCTCGGAGCATCCAGTAC; TGTGTTCCCCGAGACGATGCCGCCCCCGAGGCAGATGGCCAGGCTGCCGGCGCCCTGGCC; TCTCGCAACACCCACTCGGCTGAGCTCCCACCCCCAGACCAGCCCTCTCCGTGCAAATGC; ACATGCAAGAAGGAGCCCTCTTCGGTGTCCCCGAGCGCCACGGTCAAGACCCGCAGCACC; AAAGCGCCGCCCCCGCACCTGCCCCTGTCGCCGGCCCTGACCCGGGCGGTGGAGGGCGTC; CAGTACATTGCAGACCACCTGAAGGCCGAAGACACAGACTTCTCGGTGAAGGAGGACTGG; AAGTACGTGGCCATGGTCATCGACCGCATCTTCCTCTGGATGTTCATCATCGTCTGCCTG; CTGGGGACGGTGGGCCTCTTCCTGCCGCCCTGGCTGGCTGGCATGATCTAGGAAGGGACC; GGGAGCCTGCGTGGCCTGGGGCTGCCGTGCACGGGGCCAGCATCCATGCGGCCGGCCTGG; GGCCGGGCTGGCTTCTCCCTGGACTCTGTGGGGCCACACGTTTGCCAAATTTTCCTTCCT; GTTCTGTGTCTGCTGTAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000744 unedited; ACTATAGGGGCGGGCCGCGCAACTCATCTANAGCCCGCGAGGCGCGCGCGCCATGGAGCT; AGGGGGCCCCGGAGCGCCGCGGCTGCTGCCGCCGCTGCTGCTGCTTCTGGGGACCGGCCT; CCTGCGCGCCAGCAGCCATGTGGAGACCCGGGCCCACGCCGAGGAGCGGCTCCTGAAGAA; ACTCTTCTCCGGTTACAACAAGTGGTCCCGACCCGTGGCCAGCATCTCGGACGTGGTCCT; CGTCCGCTTCGGCCTGTCCATCGCTCAGCTCATTGACGTGGATGAGAAGAACCAGATGAT; GACCACGAACGTATGGGTGAAGCAGGAGTGGCACGACTACAAGCTGCGCTGGGACCCAGC; TGACTATGAGAATGTCACCTCCATCCGCATCCCCTCCGAGCTCATCTGGCGGCCGGACAT; CGTCCTCTACAACAATGCTGACGGGGACTTCGCGGTCACCCACCTGACCAAGGCCCACCT; GTTCCATGACGGGCGGGTGCAGTGGACTCCCCCGGCCATTTACAAGAGCTCCTGCAGCAT; CGACGTCACCTTCTTCCCCTTCGACCAGCAGAACTGCACCATGAAATTCGGCTCCTGGAC; CTACGACAAGGCCAAGATCGACCTGGTGAACATGCACAGCCGCGTGGACCAGCTGGACTT; CTGGGAGAGTGGCGAGTGGGTCATCGTGGACGCCGTGGGCACCTACCACACCAGGGAGTA; CGAGTGCTGCGCCGAGATCTACCCNGACATCACCTATGCCTTCGTCATCCGGCGGCTGCC; GCTCTTCTACCCATCAACCTCATCATCCCCTGCCTGCTCATCTCCTGCCTCACCGTGCTG; GTCTTCTACCTGCCCTCCCGATT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000744 unedited; AACACGGCAGAAGGGCCAAACTTCACAAGGCCAAGGGAGAGAGCACCTGGGGTAGGGGCT; CACAGGGTATGCCACCCGGGCTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTA; GAGTCGAGTTACAGCAGACACAGAACAGGAAGGAAAATTTGGCAAACGTGTGGCCCCACA; GAGTCCAGGGAGAAGCCAGCCCGGCCCCAGGCCGGCCGCATGGATGCTGGCCCCGTGCAC; GGCAGCCCCAGGCCACGCAGGCTCCCGGTCCCTTCCTAGATCATGCCAGCCAGCCAGGGC; GGCAGGAAGAGGCCCACCGTCCCCAGCAGGCAGACGATGATGAACATCCAGAGGAAGATG; CGGTCGATGACCATGGCCACGTACTTCCAGTCCTCCTTCACCGAGAAGTCTGTGTCTTCG; GCCTTCAGGTGGTCTGCAATGTACTGGACGCCCTCCACCGCCCGGGTCAGGGCCGGCGAC; AGGGGCAGGTGCGGGGGCGGCGCTTTGGTGCTGCGGGTCTTGACCGTGGCGCTCGGGGAC; ACCGAAGAGGGCTCCTTCTTGCATGTGCATTTGCACGGAGAGGGCTGGTCTGGGGGTGGG; AGCTCAGCCGAGTGGGTGTTGCGAGAGGCCAGGGCGCCGGCAGCCTGGCCATCTGCCTCG; GGGGCGGCATCGTCTCGGGGAACACAGTACTGGATGCTCCGAGACCGGCACCGGACGCCG; NCTTCCACCGCTTCGCCAGGGCTGGACATGTGCTGGACGCTGAGGGACCTGGCTTTGGCC; AGCCCTGGTGCCCTGGTGCCNTGGGGCGGGCGGCAAGGTCCCAGCCAGGGGTGGGGGCTG; GCTTTCTTAGCTTCCAGGGGCTGCTTGAGAGGGGACTGGTCGGAGGGTGACTTGCAGGAA; AGCCAAGGCTCAGCCCGGCCATC;
• Restriction Sites: Please inquire
• ACCN: NM_000744
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This RT-PCR clone has 4 nucleotide differences from the NCBI reference ORF. These result in the substitution of no amino acids. While there is a new version, NM_000744.3, the ORF has not changed between versions.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000744.2, NP_000735.1
• RefSeq Size: 3773 bp
• RefSeq ORF: 1884 bp
• Locus ID: 1137
• UniProt ID: P43681
• Cytogenetics: 20q13.33
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Summary: This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]; Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.