OSTM1 (NM_014028) Human Untagged Clone

SKU SC128280

OSTM1 (untagged)-Human osteopetrosis associated transmembrane protein 1 (OSTM1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: OSTM1
• Synonyms: GIPN; GL; HSPC019; OPTB5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128280 sequence for NM_014028 edited (data generated by NextGen Sequencing); ATGGAGCCGGGCCCGACAGCCGCGCAGCGGAGGTGTTCGTTGCCGCCGTGGCTGCCGCTG; GGGCTGCTGCTGTGGTCGGGGCTGGCCCTGGGCGCGCTCCCCTTCGGCAGCAGTCCGCAC; AGGGTCTTCCACGACCTCCTGTCGGAGCAGCAGTTGCTGGAGGTGGAGGACTTGTCCCTG; TCCCTCCTGCAGGGTGGAGGGCTGGGGCCTCTGTCGCTGCCCCCGGACCTGCCGGATCTG; GATCCTGAGTGCCGGGAGCTCCTGCTGGACTTCGCCAACAGCAGCGCAGAGCTGACAGGG; TGTCTGGTGCGCAGCGCCCGGCCCGTGCGCCTCTGTCAGACCTGCTACCCCCTCTTCCAA; CAGGTCGTCAGCAAGATGGACAACATCAGCCGAGCCGCGGGGAATACTTCAGAGAGTCAG; AGTTGTGCCAGAAGTCTCTTAATGGCAGATAGAATGCAAATAGTTGTGATTCTCTCAGAA; TTTTTTAATACCACATGGCAGGAGGCAAATTGTGCAAATTGTTTAACAAACAACAGTGAA; GAATTATCAAACAGCACAGTATATTTCCTTAATCTATTTAATCACACCCTGACCTGCTTT; GAACATAACCTTCAGGGGAATGCACATAGTCTTTTACAGACAAAAAATTATTCAGAAGTA; TGCAAAAACTGCCGTGAAGCATACAAAACTCTGAGTAGTCTGTACAGTGAAATGCAAAAA; ATGAATGAACTTGAGAATAAGGCTGAACCTGGAACACATTTATGCATTGATGTGGAAGAT; GCAATGAACATCACTCGAAAACTATGGAGTCGAACTTTCAACTGTTCAGTCCCTTGCAGT; GACACAGTGCCTGTAATTGCTGTTTCTGTGTTCATTCTCTTTCTACCTGTTGTCTTCTAC; CTTAGTAGCTTTCTTCACTCAGAGCAAAAGAAACGCAAACTCATTCTGCCCAAACGTCTC; AAGTCCAGTACCAGTTTTGCAAATATTCAGGAAAATTCAAACTGA; ; Clone variation with respect to NM_014028.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014028 unedited; TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGTGCCGGCTGGGGGCGG; AGAGGCGGCGGTGGGCTCCCTGGGGTGTGTGAGCCCGGTGATGGAGCCGGGCCCGACAGC; CGCGCAGCGGAGGTGTTCGTTGCCGCCGTGGCTGCCGCTGGGGCTGCTGCTGTGGTCGGG; GCTGGCCCTGGGCGCGCTCCCCTTCGGCAGCAGTCCGCACAGGGTCTTCCACGACCTCCT; GTCGGAGCAGCAGTTGCTGGAGGTGGAGGACTTGTCCCTGTCCCTCCTGCAGGGTGGAGG; GCTGGGGCCTCTGTCGCTGCCCCCGGACCTGCCGGATCTGGATCCTGAGTGCCGGGAGCT; CCTGCTGGACTTCGCCAACAGCAGCGCAGAGCTGACAGGGTGTCTGGTGCGCAGCGCCCG; GCCCGTGCGCCTCTGTCAGACCTGCTACCCCCTCTTCCAACAGGTCGTCAGCAAGATGGA; CAACATCAGCCGAGCCGCGGGGAATACTTCAGAGAGTCAGAGTTGTGCCAGAAGTCTCTT; AATGGCAGATAGAATGCAAATAGTTGTGATTCTCTCAGAATTTTTTAATACCACATGGCA; GGAGGCAAATTGTGCAAATTGTTTAACAAACAACAGTGAAGAATTATCAAACAGCACAGT; ATATTTCCTTAATCTATTTAATCACACCCTGACCTGCTTTGAACATAACCTTCAGGGGAA; TGCACATAGTCTTTTACAGACAAAAATTATTCAGAAGTATGCANAAACTGCCGTGAAGCA; TACAAACTCTGAGTAGTCTGTACAGTGAAATGCANAAATGAATGAAACTGAGATAAGGCT; GACCTGAACACATTATGCATGTTGGGGAAGAGCATGACATACTCGAAACTATGAGTCGAC; TTCAACTGTCATNCCTGANNGACANGCCGNNATGCCNGNNNCTGGGTCATCCTTTCCACT; GTGGCTCTACTT;
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_014028 unedited; AGGGCGCGGACCCTCCGGGCCGGAAGGCACCGGGGAGGGGTACAGGGACGCCACCCGGGA; TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTT; TTTTACAATCTCCTTATTTCCTTTATTTTAATGTGTCAAAAAAACACCTAAAGATATTCT; TGTAAATACATATAAGCTGTGTGTCAACATTCAGTACTATGCAAATCATTTTTCAATATG; ACAAAATGAAAAACTCACACACTTTAGGGTAGCGCTTAATACTTATCTTTGAAATCTATT; GCTGATGCTAGGTCTAAAGAGCAATGACTCAACCAGAAAAAATAGTAAAGGCTGCCTTTT; CCTTTTTAAAGTGCTTATTAGCTTTATATCCAAAAACAATGGTTTTTACAAATACATAAT; ACTGAAAGGTGCTCAAAAAGTCACCACTTACAGAATTGAACATGTCATTTTCTAACTCTG; CACATGTAAACTTGTTTTATCTGCATTAATGAAGATTGCTTCAAATGGCTCTCAATCATA; TGCTTCAAATCAAGACAGTGCTAAGTTCCAGCAGCATAAACAGTGACAGCAGAACAAACC; CCAGCACATTTTCAGTGGATCACAGCAGATCAGAGAAAGTGTAACAGCTTTTGAGTATGT; TATACATCCAAGAGCCTTATATTTCACTCCACATAAACAGTAAACATAAAAGAAAAAAGA; AACACTTTGTCTCCCTATAGAGCATTTTATAGTATAGGAAGGGATTCAAATTCTCTTTTT; AACAAAATTCTTGTTTCTATATAGAGGAGATTTTATATTCTTAATGTTTACACAAGCCTA; TTTCCACCAAAAAGCAGAGAAATAATAAATGAATCCTGACTCTAAAACCTAAGAAATAAA; ACTGTGAACAATTCTGAACGGGAGTGTTTTTTTCTAAGGCTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_014028
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014028.3, NP_054747.2
• RefSeq Size: 4467 bp
• RefSeq ORF: 1005 bp
• Locus ID: 28962
• UniProt ID: Q86WC4
• Cytogenetics: 6q21
• Protein Families: Transmembrane
• Summary: This gene encodes a protein that may be involved in the degradation of G proteins via the ubiquitin-dependent proteasome pathway. The encoded protein binds to members of subfamily A of the regulator of the G-protein signaling (RGS) family through an N-terminal leucine-rich region. This protein also has a central RING finger-like domain and E3 ubiquitin ligase activity. This protein is highly conserved from flies to humans. Defects in this gene may cause the autosomal recessive, infantile malignant form of osteopetrosis. [provided by RefSeq, Jul 2008]