AADACL1 (NCEH1) (NM_020792) Human Untagged Clone

SKU SC128225

NCEH1 (untagged)-Human neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: AADACL1
• Synonyms: AADACL1; NCEH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020792, the custom clone sequence may differ by one or more nucleotides; ; ; AGCGGAGAGCAGCGACAGAGCCTTCCTCAAACCTGTAGTGACTGCCACACTTTGCAAGGACACCGTAGAG; GGGGCATGTCCGCGCTCCAACTTCCTCCCGACGCAGCCTCTGATTGGCTCCTGGGCTTATAAGAAACGCG; TGAATGAGCAGCTGCCGCGGGCAGAAAGTTGCCGGAGGTCTCCGGGTGGTATCGCCCTTTCCTCTTTGCC; AGCCCGCTGGCGAGCCGAGCCGGGGCAAGATGAGGTCGTCCTGTGTCCTGCTCACCGCCCTGGTGGCGCT; GGCCGCCTATTACGTCTACATCCCGCTGCCTGGCTCCGTGTCCGACCCCTGGAAGCTGATGCTGCTGGAC; GCCACTTTCCGGGGTGCACAGCAAGTGAGTAACCTGATCCACTACCTGGGACTGAGCCATCACCTGCTGG; CACTGAATTTTATCATTGTTTCTTTTGGCAAAAAAAGCGCGTGGTCTTCTGCCCAAGTGAAGGTGACCGA; CACAGACTTTGATGGTGTGGAAGTCAGAGTGTTTGAAGGCCCTCCGAAGCCCGAAGAGCCACTGAAACGC; AGCGTCGTTTATATCCACGGAGGAGGCTGGGCCTTGGCAAGTGCAAAAATCAGGTATTATGATGAGCTGT; GTACAGCAATGGCTGAGGAATTGAATGCTGTCATTGTTTCCATTGAATACAGGCTAGTTCCAAAGGTTTA; TTTTCCTGAGCAAATTCATGATGTTGTACGGGCCACAAAGTATTTCCTGAAGCCAGAAGTCTTACAGAAG; TATATGGTTGATCCAGGCAGAATTTGCATTTCTGGTGACAGTGCTGGTGGAAATCTGGCTGCTGCCCTTG; GACAACAGTTTACTCAAGATGCCAGCCTAAAAAATAAGCTCAAACTACAAGCTTTAATTTATCCAGTTCT; TCAAGCTTTAGATTTTAACACACCATCTTATCAGCAAAATGTGAACACCCCAATCCTGCCCCGCTATGTC; ATGGTGAAGTATTGGGTGGACTACTTCAAAGGCAACTATGACTTTGTGCAGGCAATGATCGTTAACAATC; ACACTTCACTTGATGTGGAAGAGGCTGCTGCTGTCAGGGCCCGTCTAAACTGGACATCCCTCTTGCCTGC; ATCCTTCACAAAGAACTACAAGCCTGTTGTACAGACCACAGGCAATGCCAGGATTGTCCAGGAGCTTCCT; CAGTTGCTGGATGCCCGCTCCGCCCCACTCATTGCAGACCAGGCAGTGCTGCAGCTCCTCCCAAAGACCT; ACATTCTGACGTGTGAGCATGATGTCCTCAGAGACGATGGCATCATGTATGCCAAGCGTTTGGAGAGTGC; CGGTGTGGAGGTGACCCTGGATCACTTTGAGGATGGCTTTCACGGATGTATGATTTTCACTAGCTGGCCC; ACCAACTTCTCAGTGGGAATCCGGACTAGGAATAGTTACATCAAGTGGCTAGATCAAAACCTGTAAAGGA; GCAAAACTTCCAGAAGCCTCGAGCCCCTCTTGACCTCCTACACCTGCTTTGGAAAGACATGCACTTTTTA; GTTGACTAATTCTTCCTCCCATTCCCCTCTACTTGCGAGTTATGGAATTTCTATTCCATAACTGAAGTCT; TTATGATAACCTAATTTTTAAAAATGAATTTGACTAACTTAAGTGCAAAACATGTAAATTTGGTTCCCAG; AGTGGGCCAATCTCTCTGTTCTTGTTATCTTAGCCAACTATACTGATACCTACAGCTACAGAAAGCAGGA; CTAGGAACTGGAAATAACTTTGGGTCCTGCCTTCATTAGGACGTTCTTTTTAGAAGCAGTTCTTCCAGCT; CTGGATCATAGAGTGACCTTTAATAAGTTAAAAAAACGAGGACTCCTTAATTCTGCTAGAGTTAACCTTG; AGTTCAGAGCAGTATTAAATGCGTGCACTTTCAGGTCAGTACTGGGGACCAAGTACCCTCTGGTCTTTTG; TGAATGGATGGTTTTGTTTCCTATGGGAATTTTGGCAAAGGTTTTCTGGAAAGAACAAGTTTCTCAAAGG; ACTTTCTTCCTCTAGAATGTTCATTTTATGAGATCGCTATCTGTAAGTCCAGTTGGATTACAGGAATACT; TGAAAGTTACTTTCTACCACTATTAGAAAATATGAAGTCGCATGCACTGGATATCTATATATCATTAGGT; TTTTGTTGTGTTTTTGGTTATGCTGTCCCCCTTCTCCTTGGGGAGATATTTGGGAGCAAACTTATTTAGA; TTTAGAGTAAACTTTTCATTATAGAGCAAGTAAAAACAGACAAATGAAACAACCTAGTGTTTCACATAAA; AATACTTCTGACATAAAGTACCAAGAGCAGTGTGAATATACTTGGCATAGTCAAAAAAGAAAATACATTT; AATATTAGTTCAAAATTGTTAAAAATACCTTTAGAAGGTCTAGTCTATTATTGAAAACTCAATTTTTTCA; CTTATATGGCTTTAAAATGGAGCTATTTTGCTACAATATAATGTATTGTTTATTTTTTTAAGTTATTTAA; TGTTAATATACATAGCTAGACTTAAGGTTTTTCAGAAAGATGTCCATAATAAATATTAAAAACAATGGTA; TTTTTTAAAAAACTGCCTTAGGGTTTTAAAACCTTCCCTACAGTTATAACCACGTGTAATTTTGTGGAAA; TGATATAACAGCTATTAATACTACTATAACATATGCATAAATATTTTCGTGTTTATATGCATATACAAGT; TAAAATAATTAGAAACTATGACTGCGCCTAGTAAAGTCATCTAGGTTTATAGTTCAGTAGCTTAGGCAAG; GCACACACTGCTCATCTCCGCTTTTTAGGGTCAGAGGAACACAAGCTCATGTTCTGAGTGAAGGGCGTAC; ACTGGCACCTGGTGTTGCCTAGATCCCCCATCTCCTCCTTCCAGCCAGGTCTGGAAGTTTCAACAGCCCA; AGCTTAACTTCATGTAAAGTCTTCACTGCCAGTGGGAACATCTTTGACACAACAAGACACTCCAATTGTG; ATTTGAGTTGAGGATCTCTGCCTGCCTTCCTGCCGTCCTTCCTTCTTCCGCGATCCATGCTACTTTTAGG; GGCTGCGGAGAGCAGCAGCAGAGCTGAGTAATGATACAGGGCACCACGGAGAGAAAGTAGAACCATTTCA; CTCCTGGGAAGATGGGGTATTTCCCACTTCCAGCAACGAAATAACAAATGAAAAGTTGCATACTTATTGA; TGTATTGTATGAGCCAGTAGCATTTTATGTACAAAACAGAAGTCAATGCAACAGTATGTATGTGTGCCTG; TGTGTGTATAAAAATAACCATTGAAGCTAACTTGCTAATGTACTTAGGCAAGCCACTTCCCATCTCTGGG; CCTCGTCTTTCCTCCCTCTAAAATCAAAGAGCTGAATTATGTGATCCTTGAGGTCTCTTCCACTTATAAT; ACCAACTGTCTTGTCAGACTGGCAAATTATATTGGCCTCTCCTTATGTGGTGGTTTTTTTGGTAGGTCAT; AGTTCCTTATACACAGACACCTGCTCATCGAAGGTCTTTTTTTTCCTAAAAAAAAAAAATGGGATTTTAG; TTCTTATTCTGTGATAACTATCCTCCTCATATAATACTATTCTTTTTGACACCATTTGAAGGAACCAATA; TTTGGACCTTATTTTGAGGTTGTCTGTCTCGAAGAAAAAGAAAATAAAATGTATAGGCAGGGTTCCTTCA; ATTGGCATTTTCCCCAGAATTGTGAGCCAAAGCCTATAGTAATTGCAGACAGCAAATGATTCCGGATCTC; TAAAAGGCTCTCTCAGATGAAAAGGGAGTAAAGGAAAAAAGAGGTCAACCACTGTTTCTGATAATGTACT; TGAGTTTCATTGTTCTTTTAGTTTGTATTCTTATAAAAAATGTTTACACTCTGCAGATTGATTTTTTTTT; TTTTAGTACTGTGGCTTTCTTTTCCTATTTTATGAAAAAAATGATAATCTTTTTGTAAAATTGTCTGTGA; AATATAAACATTAATATATAAAGAAAAACCTTGAAGTGCTGTATAGTGAAGTATAAATTAATGTTTTATT; GATTTGTGAAGAATTTAAGACTATTATATAATTATCTTGGTGGATCTATTTTATGCATGACCTTTTAACC; TTTGACTTTGCTTATTTCCCACTACGAAGGGGAAGGTAGATTTTATGAATGATTTTAATAGCAAATATAT; TTTATAAAGTGAAAATCCAGTGTGGAGGTAGCAAAGCATCTATCTATTCTGAATCATGTTTGGAAATAAA; ATTGCTCCATCTGGGAATGTGCTTTCAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020792 unedited; NNTTTTGTCCAGGTGCATAATTTGTNATACCACTCACTATAGGCGGCCGCATAACTTCGT; ATAGCATACATTATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGA; GAGCGGAGAGCAGCGACAGAGCCTTCCTCAAACCTGTAGTGACTGCCACACTTTGCAAGG; ACACCGTAGAGGGGGCATGTCCGCGCTCCAACTTCCTCCCGACGCAGCCTCTGATTGGCT; CCTGGGCTTATAAGAAACGCGTGAATGAGCAGCTGCCGCGGGCAGAAAGTTGCCGGAGGT; CTCCGGGTGGTATCGCCCTTTCCTCTTTGCCAGCCCGCTGGCGAGCCGAGCCGGGGCAAG; ATGAGGTCGTCCTGTGTCCTGCTCACCGCCCTGGTGGCGCTGGCCGCCTATTACGTCTAC; ATCCCGCTGCCTGGCTCCGTGTCCGACCCCTGGAAGCTGATGCTGCTGGACGCCACTTTC; CGGGGTGCACAGCAAGTGAGTAACCTGATCCACTACCTGGGACTGAGCCATCACCTGCTG; GCACTGAATTTTATCATTGTTTCTTTTGGCAAANAAAGCGCGTGGTCTTCTGCCCAAGTG; AAGGTGACCGACACAGACTTTGATGGTGTGGAAGTCAGAGTGTTTGAAGGCCCCTCGAAG; CCCGAGAGCCACTGAAACGCAGCGTCGTTTATATCCCCACGAGAGGCTGGGCCTTGGNCA; GTGCAAAAATCANGTATTATGATGAGCTGTGTACAGCAATGGCTGAGGAAATTGATGCTG; TCATTGTTTCCATTTGATACAGGCTAGTTCCAAAGGTTTATTTTCCTGAGCAAATCATGA; TGTTGTACGGNCCACAAATATTTTCTNGAACC;
• Restriction Sites: Please inquire
• ACCN: NM_020792
• Insert Size: 4300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020792.3, NP_065843.3
• RefSeq Size: 4289 bp
• RefSeq ORF: 1323 bp
• Locus ID: 57552
• UniProt ID: Q6PIU2
• Cytogenetics: 3q26.31
• Protein Families: Transmembrane
• Summary: Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform b) compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by available conservation data.