M-CSF (CSF1) (NM_000757) Human Untagged Clone

CAT#: SC128208

CSF1 (untagged)-Human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: M-CSF
• Synonyms: CSF-1; MCSF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128208 sequence for NM_000757 edited (data generated by NextGen Sequencing)
  ATGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGCTCCCTGCTG
  TTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAGTACTGTAGC
  CACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGTCAGATGGAG
  ACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGATCCAGTGTGC
  TACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACACCATGCGCTTCAGA
  GATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTGAGGCTGAAG
  AGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGCCTGCGTCCGAACTTTCTATGAG
  ACACCTCTCCAGTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAACAAAGAATCTCCTT
  GACAAGGACTGGAATATTTTCAGCAAGAACTGCAACAACAGCTTTGCTGAATGCTCCAGC
  CAAGATGTGGTGACCAAGCCTGATTGCAACTGCCTGTACCCCAAAGCCATCCCTAGCAGT
  GACCCGGCCTCTGTCTCCCCTCATCAGCCCCTCGCCCCCTCCATGGCCCCTGTGGCTGGC
  TTGACCTGGGAGGACTCTGAGGGAACTGAGGGCAGCTCCCTCTTGCCTGGTGAGCAGCCC
  CTGCACACAGTGGATCCAGGCAGTGCCAAGCAGCGGCCACCCAGGAGCACCTGCCAGAGC
  TTTGAGCCGCCAGAGACCCCAGTTGTCAAGGACAGCACCATCGGTGGCTCACCACAGCCT
  CGCCCCTCTGTCGGGGCCTTCAACCCCGGGATGGAGGATATTCTTGACTCTGCAATGGGC
  ACTAATTGGGTCCCAGAAGAAGCCTCTGGAGAGGCCAGTGAGATTCCCGTACCCCAAGGG
  ACAGAGCTTTCCCCCTCCAGGCCAGGAGGGGGCAGCATGCAGACAGAGCCCGCCAGACCC
  AGCAACTTCCTCTCAGCATCTTCTCCACTCCCTGCATCAGCAAAGGGCCAACAGCCGGCA
  GATGTAACTGGTACAGCCTTGCCCAGGGTGGGCCCCGTGAGGCCCACTGGCCAGGACTGG
  AATCACACCCCCCAGAAGACAGACCATCCATCTGCCCTGCTCAGAGACCCCCCGGAGCCA
  GGCTCTCCCAGGATCTCATCACTGCGCCCCCAGGGCCTCAGCAACCCCTCCACCCTCTCT
  GCTCAGCCACAGCTTTCCAGAAGCCACTCCTCGGGCAGCGTGCTGCCCCTTGGGGAGCTG
  GAGGGCAGGAGGAGCACCAGGGATCGGAGGAGCCCCGCAGAGCCAGAAGGAGGACCAGNN
  NNNNNNNNNGCAGCCAGGCCCCTGCCCCGTTTTAACTCCGTTCCTTTGACTGACACAGGC
  CATGAGAGGCAGTCCGAGGGATCCTCCAGCCCGCAGCTCCAGGAGTCTGTCTTCCACCTG
  CTGGTGCCCAGTGTCATCCTGGTCTTGCTGGCCGTCGGAGGCCTCTTGTTCTACAGGTGG
  AGGCGGCGGAGCCATCAAGAGCCTCAGAGAGCGGATTCTCCCTTGGAGCAACCAGAGGGC
  AGCCCCCTGACTCAGGATGACAGACAGGTGGAACTGCCAGTGTAG
  
  Clone variation with respect to NM_000757.5
  1095 c=>a;1379 c=>n;1380 a=>n;1381 a=>n;1382 g=>n;1383 t=>n;1384 g=>n;1385 a=>n;1386 a=>n;1387 g=>n;1388 g=>n;1389 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000757 unedited
  GTTCGGATTTGTATACGACTTATATAGGGCGGCCGCGAATTCGCACGAGGGAGGGCTGGC
  CAGTGAGGCTCGGCCCGGGGAAAGTGAAAGTTTGCCTGGGTCCTCTCGGCGCCAGAGCCG
  CTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTCCGCAGCAGCC
  AGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCAGCTGCCCGTA
  TGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGCTCCCTGCTGT
  TGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAGTACTGTAGCC
  ACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGTCAGATGGAGA
  CCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGATCCAGTGTGCT
  ACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGGACACCATGCGCTTCAGA
  GATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTGAGGCTGGAA
  GAGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGCCTGCGTCCGAACTTCCTATGG
  AGACACCTCTCCATTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAACAAAGAATCTCC
  TTGACAGGGACTGGGAATATTTTCAGCAAGAACTGCAACAACAGCCTTGCTGAGTGCTCC
  ACCCAGAAGTGGTGACCAAGCCTGATGGCAATGCCTGTGCCCCAAAACCATCCCTAGCAG
  TGACCCGGCCTCTGGGTTCCCTAATAAGCCCCTTGCCCCCTCCTGGGGCCTGGGGGCTGG
  GCTGACCGGGGAGGACTCTGAGGGAACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000757 unedited
  AGAGCTGGNATAGTGGAGACGGTCGGTTGCTGGCTCATGGATGGACCGGGGCAGGCTGTT
  CAGCAAGCAGGTACAGTGTTCACAGTGCAATGGCTTCCTTCATGAGGGGAGCCTCTGCTT
  GGCCTCCTGGAGAAGCTCTGGCAGGTGCTGGAAGGAGGAGGGGCAGAGGCCTGTGCTTGA
  TCCCCCTCTTGGCATCACTGGCAGGTCAGGCAGGTCCTGAGGGCAGGGCTGGCCTCCCCT
  CTGCGCCTGGCAACCAGCACAGGGCACTTATGTGAGCCGAGGGTGTAGACCCTTCAGGCT
  GTCAGTGCAGACCAGCAGGTCCAGAGGCCTCCTCTTCCCGTCTCTCAGGCTGCACACCTT
  TACGGGAAATCAGGCCGACGCTGTCCCGCCCCACGAACTTCAGGCTCCTCTCTCTGGAGA
  CAGGTCCATGCCTGTCCAGGCTGGGATACCTTGGCACCAGGCAGGCCCTCTGGGTGCAGG
  GTTCTTTCACTCCTCAGCGTCCACCCTGCTTTCCCACAAACCACAAGCCGGTGGGTCCCT
  GGGCCTGGGTTCCGGCATGGAAGGGAGTGAGAGGAGGGTGCAGCCCCTGAGGGAAGAGGG
  TCCTGAGCCGGGGTCACCAACCTTTCTCAAGCACATGGGGAGCCTCCCAGGGCTCACAAT
  AAATAGTGGCACCCCTGGGGAGCATCCCTCTGGGCTGTTTTCTTTCATTCAAAGGGTCTG
  CCGGGTTGGGACAAAAGGGCCCCCAGCCCGGCCTGCCCTGGTCCCAATCCCTGGAAGCCA
  GTACCTCTGGGTGAGGGGCAAACCACATTCTCAGAGA
  
• Restriction Sites: Please inquire
• ACCN: NM_000757
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000757.3, NP_000748.3
• RefSeq Size: 2749 bp
• RefSeq ORF: 1665 bp
• Locus ID: 1435
• UniProt ID: P09603
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage
• Gene Summary: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses a downstream in-frame AUG start codon, which is supported by homology and publication data. The use of the 5'-most in-frame AUG in the transcript would result in a 74 aa N-terminal extension, but this N-terminal extension is not conserved in other species, and it would eliminate an N-terminal signal peptide, which is known to be present based on experimental evidence (PMIDs 8262907, 3498652).