MCK10 (DDR1) (NM_013994) Human Untagged Clone

SKU SC128202

DDR1 (untagged)-Human discoidin domain receptor tyrosine kinase 1 (DDR1), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: MCK10
• Synonyms: CAK; CD167; DDR; EDDR1; HGK2; MCK10; NEP; NTRK4; PTK3; PTK3A; RTK6; TRKE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013994, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGGACCAGAGGCCCTGTCATCTTTACTGCTGCTGCTCTTGGTGGCAAGTGGAGATGCTGACATGAAGG; GACATTTTGATCCTGCCAAGTGCCGCTATGCCCTGGGCATGCAGGACCGGACCATCCCAGACAGTGACAT; CTCTGCTTCCAGCTCCTGGTCAGATTCCACTGCCGCCCGCCACAGCAGGTTGGAGAGCAGTGACGGGGAT; GGGGCCTGGTGCCCCGCAGGGTCGGTGTTTCCCAAGGAGGAGGAGTACTTGCAGGTGGATCTACAACGAC; TGCACCTGGTGGCTCTGGTGGGCACCCAGGGACGGCATGCCGGGGGCCTGGGCAAGGAGTTCTCCCGGAG; CTACCGGCTGCGTTACTCCCGGGATGGTCGCCGCTGGATGGGCTGGAAGGACCGCTGGGGTCAGGAGGTG; ATCTCAGGCAATGAGGACCCTGAGGGAGTGGTGCTGAAGGACCTTGGGCCCCCCATGGTTGCCCGACTGG; TTCGCTTCTACCCCCGGGCTGACCGGGTCATGAGCGTCTGTCTGCGGGTAGAGCTCTATGGCTGCCTCTG; GAGGGATGGACTCCTGTCTTACACCGCCCCTGTGGGGCAGACAATGTATTTATCTGAGGCCGTGTACCTC; AACGACTCCACCTATGACGGACATACCGTGGGCGGACTGCAGTATGGGGGTCTGGGCCAGCTGGCAGATG; GTGTGGTGGGGCTGGATGACTTTAGGAAGAGTCAGGAGCTGCGGGTCTGGCCAGGCTATGACTATGTGGG; ATGGAGCAACCACAGCTTCTCCAGTGGCTATGTGGAGATGGAGTTTGAGTTTGACCGGCTGAGGGCCTTC; CAGGCTATGCAGGTCCACTGTAACAACATGCACACGCTGGGAGCCCGTCTGCCTGGCGGGGTGGAATGTC; GCTTCCGGCGTGGCCCTGCCATGGCCTGGGAGGGGGAGCCCATGCGCCACAACCTAGGGGGCAACCTGGG; GGACCCCAGAGCCCGGGCTGTCTCAGTGCCCCTTGGCGGCCGTGTGGCTCGCTTTCTGCAGTGCCGCTTC; CTCTTTGCGGGGCCCTGGTTACTCTTCAGCGAAATCTCCTTCATCTCTGATGTGGTGAACAATTCCTCTC; CGGCACTGGGAGGCACCTTCCCGCCAGCCCCCTGGTGGCCGCCTGGCCCACCTCCCACCAACTTCAGCAG; CTTGGAGCTGGAGCCCAGAGGCCAGCAGCCCGTGGCCAAGGCCGAGGGGAGCCCGACCGCCATCCTCATC; GGCTGCCTGGTGGCCATCATCCTGCTCCTGCTGCTCATCATTGCCCTCATGCTCTGGCGGCTGCACTGGC; GCAGGCTCCTCAGCAAGGCTGAACGGAGGGTGTTGGAAGAGGAGCTGACGGTTCACCTCTCTGTCCCTGG; GGACACTATCCTCATCAACAACCGCCCAGGTCCTAGAGAGCCACCCCCGTACCAGGAGCCCCGGCCTCGT; GGGAATCCGCCCCACTCCGCTCCCTGTGTCCCCAATGGCTCTGCGTTGCTGCTCTCCAATCCAGCCTACC; GCCTCCTTCTGGCCACTTACGCCCGTCCCCCTCGAGGCCCGGGCCCCCCCACACCCGCCTGGGCCAAACC; CACCAACACCCAGGCCTACAGTGGGGACTATATGGAGCCTGAGAAGCCAGGCGCCCCGCTTCTGCCCCCA; CCTCCCCAGAACAGCGTCCCCCATTATGCCGAGGCTGACATTGTTACCCTGCAGGGCGTCACCGGGGGCA; ACACCTATGCTGTGCCTGCACTGCCCCCAGGGGCAGTCGGGGATGGGCCCCCCAGAGTGGATTTCCCTCG; ATCTCGACTCCGCTTCAAGGAGAAGCTTGGCGAGGGCCAGTTTGGGGAGGTGCACCTGTGTGAGGTCGAC; AGCCCTCAAGATCTGGTTAGTCTTGATTTCCCCCTTAATGTGCGTAAGGGACACCCTTTGCTGGTAGCTG; TCAAGATCTTACGGCCAGATGCCACCAAGAATGCCAGCTTCTCCTTGTTCTCCAGGAATGATTTCCTGAA; AGAGGTGAAGATCATGTCGAGGCTCAAGGACCCAAACATCATTCGGCTGCTGGGCGTGTGTGTGCAGGAC; GACCCCCTCTGCATGATTACTGACTACATGGAGAACGGCGACCTCAACCAGTTCCTCAGTGCCCACCAGC; TGGAGGACAAGGCAGCCGAGGGGGCCCCTGGGGACGGGCAGGCTGCGCAGGGGCCCACCATCAGCTACCC; AATGCTGCTGCATGTGGCAGCCCAGATCGCCTCCGGCATGCGCTATCTGGCCACACTCAACTTTGTACAT; CGGGACCTGGCCACGCGGAACTGCCTAGTTGGGGAAAATTTCACCATCAAAATCGCAGACTTTGGCATGA; GCCGGAACCTCTATGCTGGGGACTATTACCGTGTGCAGGGCCGGGCAGTGCTGCCCATCCGCTGGATGGC; CTGGGAGTGCATCCTCATGGGGAAGTTCACGACTGCGAGTGACGTGTGGGCCTTTGGTGTGACCCTGTGG; GAGGTGCTGATGCTCTGTAGGGCCCAGCCCTTTGGGCAGCTCACCGACGAGCAGGTCATCGAGAACGCGG; GGGAGTTCTTCCGGGACCAGGGCCGGCAGGTGTACCTGTCCCGGCCGCCTGCCTGCCCGCAGGGCCTATA; TGAGCTGATGCTTCGGTGCTGGAGCCGGGAGTCTGAGCAGCGACCACCCTTTTCCCAGCTGCATCGGTTC; CTGGCAGAGGATGCACTCAACACGGTGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013994 unedited; GGGAAGTTCACATTTGTAAACGACTCATATAGGCGGCCGCGCAATTCGCACGAGGCCCGA; GGGATCAGGAGCTATGGGACCAGAGGCCCTGTCATCTTTACTGCTGCTGCTCTTGGTGGC; AAGTGGAGATGCTGACATGAAGGGACATTTTGATCCTGCCAAGTGCCGCTATGCCCTGGG; CATGCAGGACCGGACCATCCCAGACAGTGACATCTCTGCTTCCAGCTCCTGGTCAGATTC; CACTGCCGCCCGCCACAGCAGGTTGGAGAGCAGTGACGGGGATGGGGCCTGGTGCCCCGC; AGGGTCGGTGTTTCCCAAGGAGGAGGAGTACTTGCAGGTGGATCTACAACGACTCCACCT; GGTGGCTCTGGTGGGCACCCAGGGACGGCATGCCGGGGGCCTGGGCAAGGAGTTCTCCCG; GAGCTACCGGCTGCGTTACTCCCGGGATGGTCGCCGCTGGATGGGCTGGAAGGACCGCTG; GGGTCAGGAGGTGATCTCAGGCAATGAGGACCCTGAGGGAGTGGTGCTGAAGGACCTTGG; GCCCCCCATGGTTGCCCGACTGGTTCGCTTCTACCCCCGGGCTGACCGGGTCATGAGTGT; CTGTCTGCGGGTAGAGCTCTATGGCTGCCTCTGGAGGGATGGACTCCTGTCTTACACCGC; CCCTGTGGGGCAGACAATGTATTTATCTGAGGCCGTGTACCTCAACGACTCCACCTATGA; CGGACATACCGTGGGCGGACTGCAGTATGGGGGTCTGGGCCAGCTGGCANATGGTGTGGT; GGGGCTGGATGACTTTAGGAAAGAGTCAGAGCTGCGGGTCTGGGCAGGCTATGACTATGT; GGGAATGGAGCAACACAGCTTTCTCAGTGGCTATGTGGGAGAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013994 unedited; CGCCGACTATATGGNGGGATGGTCGAACTCTTCCGTGGNCNCAGGTNATGAGTCACTGGT; GGNAGGGTGTCACAGGGAATGCCACCCGGGATCTGTTCAGGAAAAGCTATGACCGCGGCC; GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTGTGGAAAACTCAACCTTTATTATTA; CCTGCCTATTGCATGGGATTAAAATTGCCTCAAGCTAGGTCCATATATTAGTGTAAAAAT; CTGTGTCTCTCCCCCCAAAATGTACAAACCCCAAGTGATTATATAAAAATCAATGTGGCA; GCTACACTAGAGATGTTCAACCCCAAGGCTATGGGCCGTTGCTCCCTCTTTCCCCCCAAT; CCCTATATTTACTTCACATAAACGTATACGCTTAGAGAATTTCTATCTACAAAACTGCAG; GTGGGAAGTGGGGGTGACTGAGGGCTGGGGCGGGGCTACCCCCAGGTCCACTGTTTCATG; TGATGGACGAGGAATAAGCCCAAGCTGAGGACAAGTACAGGAGCACGCACTAGGAAACAT; TTTCCTCTCCTTCTTGTCCTCCTCGCCCCCAGATTCTCATCCACTGGGGTCCAGTGTGTG; ACACGGAGAGAGAAGCTGGACGCGCATCCACCTCTCCAGGAATCAGTGTTGTCCAGTGGG; GCCCACGTGCTNNCATGGGCCATGTGCAGTGTCTATCCTATATTTCTCCCCACCCTTCCC; CACGGATCGCTAGAGGCAGGTGGAGATGATCCNTCCACAGAACCAGCTTGGTGGGCTACA; GGGGCCTTCGTGTTGAGTGCATACTCTGCCCAGAAACTATGCAACTGGGAAAAGGTGGTT; GCTGGTAANACTCCCCGGTTCAGCACCCAAACTTTGGTGATATAGGCCCTGCGGGCAGGC; AGCTGCCCGGACC;
• Restriction Sites: Please inquire
• ACCN: NM_013994
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013994.2, NP_054700.2
• RefSeq Size: 3678 bp
• RefSeq ORF: 2760 bp
• Locus ID: 780
• UniProt ID: Q08345
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Summary: Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]; Transcript Variant: This variant (3) contains an additional in-frame coding exon, and uses an alternate in-frame acceptor splice site at one of the coding exons compared to variant 1. This results in the longest isoform (3, also known as DDR1c) with additional protein segments not found in isoform 1.