PGC1 alpha (PPARGC1A) (NM_013261) Human Untagged Clone

SKU SC128185

PPARGC1A (untagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PGC1 alpha
• Synonyms: LEM6; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1A; PPARGC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013261, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGTGGGACATGTGCAACCAGGACTCTGAGTCTGTATGGAGTGACATCGAGTGTGCTGCTCTGGTTG; GTGAAGACCAGCCTCTTTGCCCAGATCTTCCTGAACTTGATCTTTCTGAACTAGATGTGAACGACTTGGA; TACAGACAGCTTTCTGGGTGGACTCAAGTGGTGCAGTGACCAATCAGAAATAATATCCAATCAGTACAAC; AATGAGCCTTCAAACATATTTGAGAAGATAGATGAAGAGAATGAGGCAAACTTGCTAGCAGTCCTCACAG; AGACACTAGACAGTCTCCCTGTGGATGAAGACGGATTGCCCTCATTTGATGCGCTGACAGATGGAGACGT; GACCACTGACAATGAGGCTAGTCCTTCCTCCATGCCTGACGGCACCCCTCCACCCCAGGAGGCAGAAGAG; CCGTCTCTACTTAAGAAGCTCTTACTGGCACCAGCCAACACTCAGCTAAGTTATAATGAATGCAGTGGTC; TCAGTACCCAGAACCATGCAAATCACAATCACAGGATCAGAACAAACCCTGCAATTGTTAAGACTGAGAA; TTCATGGAGCAATAAAGCGAAGAGTATTTGTCAACAGCAAAAGCCACAAAGACGTCCCTGCTCGGAGCTT; CTCAAATATCTGACCACAAACGATGACCCTCCTCACACCAAACCCACAGAGAACAGAAACAGCAGCAGAG; ACAAATGCACCTCCAAAAAGAAGTCCCACACACAGTCGCAGTCACAACACTTACAAGCCAAACCAACAAC; TTTATCTCTTCCTCTGACCCCAGAGTCACCAAATGACCCCAAGGGTTCCCCATTTGAGAACAAGACTATT; GAACGCACCTTAAGTGTGGAACTCTCTGGAACTGCAGGCCTAACTCCACCCACCACTCCTCCTCATAAAG; CCAACCAAGATAACCCTTTTAGGGCTTCTCCAAAGCTGAAGTCCTCTTGCAAGACTGTGGTGCCACCACC; ATCAAAGAAGCCCAGGTACAGTGAGTCTTCTGGTACACAAGGCAATAACTCCACCAAGAAAGGGCCGGAG; CAATCCGAGTTGTATGCACAACTCAGCAAGTCCTCAGTCCTCACTGGTGGACACGAGGAAAGGAAGACCA; AGCGGCCCAGTCTGCGGCTGTTTGGTGACCATGACTATTGCCAGTCAATTAATTCCAAAACAGAAATACT; CATTAATATATCACAGGAGCTCCAAGACTCTAGACAACTAGAAAATAAAGATGTCTCCTCTGATTGGCAG; GGGCAGATTTGTTCTTCCACAGATTCAGACCAGTGCTACCTGAGAGAGACTTTGGAGGCAAGCAAGCAGG; TCTCTCCTTGCAGCACAAGAAAACAGCTCCAAGACCAGGAAATCCGAGCCGAGCTGAACAAGCACTTCGG; TCATCCCAGTCAAGCTGTTTTTGACGACGAAGCAGACAAGACCGGTGAACTGAGGGACAGTGATTTCAGT; AATGAACAATTCTCCAAACTACCTATGTTTATAAATTCAGGACTAGCCATGGATGGCCTGTTTGATGACA; GCGAAGATGAAAGTGATAAACTGAGCTACCCTTGGGATGGCACGCAATCCTATTCATTGTTCAATGTGTC; TCCTTCTTGTTCTTCTTTTAACTCTCCATGTAGAGATTCTGTGTCACCACCCAAATCCTTATTTTCTCAA; AGACCCCAAAGGATGCGCTCTCGTTCAAGGTCCTTTTCTCGACACAGGTCGTGTTCCCGATCACCATATT; CCAGGTCAAGATCAAGGTCTCCAGGCAGTAGATCCTCTTCAAGATCCTGCTATTACTATGAGTCAAGCCA; CTACAGACACCGCACGCACCGAAATTCTCCCTTGTATGTGAGATCACGTTCAAGATCGCCCTACAGCCGT; CGGCCCAGGTATGACAGCTACGAGGAATATCAGCACGAGAGGCTGAAGAGGGAAGAATATCGCAGAGAGT; ATGAGAAGCGAGAGTCTGAGAGGGCCAAGCAAAGGGAGAGGCAGAGGCAGAAGGCAATTGAAGAGCGCCG; TGTGATTTATGTCGGTAAAATCAGACCTGACACAACACGGACAGAACTGAGGGACCGTTTTGAAGTTTTT; GGTGAAATTGAGGAGTGCACAGTAAATCTGCGGGATGATGGAGACAGCTATGGTTTCATTACCTACCGTT; ATACCTGTGATGCTTTTGCTGCTCTTGAAAATGGATACACTTTGCGCAGGTCAAACGAAACTGACTTTGA; GCTGTACTTTTGTGGACGCAAGCAATTTTTCAAGTCTAACTATGCAGACCTAGATTCAAACTCAGATGAC; TTTGACCCTGCTTCCACCAAGAGCAAGTATGACTCTCTGGATTTTGATAGTTTACTGAAAGAAGCTCAGA; GAAGCTTGCGCAGGTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013261 unedited; GGGGGGTTGAAGCTCTTTTCCCCGCCCGTTGCCNCAATGGGCGGTAGGCGTGTACGGTGG; GNAGTCTATATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTT; TTTGCAGCGGCCGCGAATTCGGCACGAGGGTTGCCTGCATGAGTGTGTGCTCTGTGTCAC; TGTGGATTGGAGTTGAAAAAGCTTGACTGGCGTCATTCAGGAGCTGGATGGCGTGGGACA; TGTGCAACCAGGACTCTGAGTCTGTATGGAGTGACATCGAGTGTGCTGCTCTGGTTGGTG; AAGACCAGCCTCTTTGCCCAGATCTTCCTGAACTTGATCTTTCTGAACTAGATGTGAACG; ACTTGGATACAGACAGCTTTCTGGGTGGACTCAAGTGGTGCAGTGACCAATCAGAAATAA; TATCCAATCAGTACAACAATGAGCCTTCAAACATATTTGAGAAGATAGATGAAGAGAATG; AGGCAAACTTGCTAGCAGTCCTCACAGAGACACTAGACAGTCTCCCTGTGGATGAAGACG; GATTGCCCTCATTTGATGCGCTGACAGATGGAGACGTGACCACTGACAATGAGGCTAGTC; CTTCCTCCATGCCTGACGGCACCCCTCCACCCCAGGAGGCAGAAGAGCCGTCTCTACTTA; AGAAGCTCTTACTGGCACCAGCCAACACTCAGCTAAGTTATAATGAATGCAGTGGTCTCA; GTACCCAGAACCATGCAAATCACAATCACAGGATCAGAACANACCCTGCAATTGTTAAGA; CTGAGAATTCATGGAGCAATAAAGCGAAGAGTATTTGTCAACAGCAAAAGCCACAAAGAC; GTCCCTGCTCGGAGCTTCTCAAATAT;
• Restriction Sites: Please inquire
• ACCN: NM_013261
• Insert Size: 2397 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013261.2, NP_037393.1
• RefSeq Size: 6317 bp
• RefSeq ORF: 2397 bp
• Locus ID: 10891
• UniProt ID: Q9UBK2
• Cytogenetics: 4p15.2
• Domains: RRM
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, Huntington's disease, Insulin signaling pathway
• Summary: The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.