CD44 (NM_001001391) Human Untagged Clone

SKU SC128160

CD44 (untagged)-Human CD44 molecule (Indian blood group) (CD44), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: CD44
• Synonyms: CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128160 sequence for NM_001001391 edited (data generated by NextGen Sequencing); ATGGACAAGTTTTGGTGGCACGCAGCCTGGGGACTCTGCCTCGTGCCGCTGAGCCTGGCG; CAGATCGATTTGAATATAACCTGCCGCTTTGCAGGTGTATTCCACGTGGAGAAAAATGGT; CGCTACAGCATCTCTCGGACGGAGGCCGCTGACCTCTGCAAGGCTTTCAATAGCACCTTG; CCCACAATGGCCCAGATGGAGAAAGCTCTGAGCATCGGATTTGAGACCTGCAGGTATGGG; TTCATAGAAGGGCACGTGGTGATTCCCCGGATCCACCCCAACTCCATCTGTGCAGCAAAC; AACACAGGGGTGTACATCCTCACATCCAACACCTCCCAGTATGACACATATTGCTTCAAT; GCTTCAGCTCCACCTGAAGAAGATTGTACATCAGTCACAGACCTGCCCAATGCCTTTGAT; GGACCAATTACCATAACTATTGTTAACCGTGATGGCACCCGCTATGTCCAGAAAGGAGAA; TACAGAACGAATCCTGAAGACATCTACCCCAGCAACCCTACTGATGATGACGTGAGCAGC; GGCTCCTCCAGTGAAAGGAGCAGCACTTCAGGAGGTTACATCTTTTACACCTTTTCTACT; GTACACCCCATCCCAGACGAAGACAGTCCCTGGATCACCGACAGCACAGACAGAATCCCT; GCTACCAGAGACCAAGACACATTCCACCCCAGTGGGGGGTCCCATACCACTCATGGATCT; GAATCAGATGGACACTCACATGGGAGTCAAGAAGGTGGAGCAAACACAACCTCTGGTCCT; ATAAGGACACCCCAAATTCCAGAATGGCTGATCATCTTGGCATCCCTCTTGGCCTTGGCT; TTGATTCTTGCAGTTTGCATTGCAGTCAACAGTCGAAGAAGGTGTGGGCAGAAGAAAAAG; CTAGTGATCAACAGTGGCAATGGAGCTGTGGAGGACAGAAAGCCAAGTGGACTCAACGGA; GAGGCCAGCAAGTCTCAGGAAATGGTGCATTTGGTGAACAAGGAGTCGTCAGAAACTCCA; GACCAGTTTATGACAGCTGATGAGACAAGGAACCTGCAGAATGTGGACATGAAGATTGGG; GTGTAA; ; Clone variation with respect to NM_001001391.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001001391 unedited; TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCCAGCCTCTGCCGGTT; CGGTCCGCCATCCTCGTCCCGTCCTCCGCCGGCCCCTGCCCCGCGCCCAGGGATCCTCCA; GCTCCTTTCGCCCGCGCCCTCCGTTCGCTCCGGACACCATGGACAAGTTTTGGTGGCACG; CAGCCTGGGGACTCTGCCTCGTGCCGCTGAGCCTGGCGCAGATCGATTTGAATATAACCT; GCCGCTTTGCAGGTGTATTCCACGTGGAGAAAAATGGTCGCTACAGCATCTCTCGGACGG; AGGCCGCTGACCTCTGCAAGGCTTTCAATAGCACCTTGCCCACAATGGCCCAGATGGAGA; AAGCTCTGAGCATCGGATTTGAGACCTGCAGGTATGGGTTCATAGAAGGGCACGTGGTGA; TTCCCCGGATCCACCCCAACTCCATCTGTGCAGCAAACAACACAGGGGTGTACATCCTCA; CATCCAACACCTCCCAGTATGACACATATTGCTTCAATGCTTCAGCTCCACCTGAAGAAG; ATTGTACATCAGTCACAGACCTGCCCAATGCCTTTGATGGACCAATTACCATAACTATTG; TTAACCGTGATGGCACCCGCTATGTCCAGAAAGGAGAATACAGAACGAATCCTGAAGACA; TCTACCCCAGCAACCCTACTGATGATGACGTGAGCAGCGGCTCCTCCAGTGAAAGGAGCA; GCACTTCAGGAGGTTACATCTTTTACACCTTTTCTACTGTACACCCCATCCCAGACGAAG; ACAGTCCCTGGGATCACCGACAGCACAGACAGAATCCCTGCTACCAGAGACCAAGAACAT; TNCACCCCAGGGGGGGGTCCCATACACTCATGGATCTGATCAGATGACACTCACATGGAG; TCAAGAAGTGGACANACCACCTCTGGTCTATAGGACACCCAATNCAAATGCTGATATCTN; GCATCCTCTTGGCTGN;
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_001001391 unedited; CGCAATCTATAGTCGAGTNCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTGAAAATCGAAAAAATAAAAATTTTTTTTTTGAATCAATTTAAACC; CTTTTGGGCCTTTGACAAAGTAACTTTAAAAAATTTTTCTGGTCAAAGGGCTGGTCCAGG; GGTTAATTTTTCAAAAACCCCAATAAATTGTTTACTACAAATAAATAACTTCACCCTTTG; GGGGGCTCCCAAAAACATTTGAAAAATTTCTAAAGGGGGGCTGTTGAAAATTTGTAACTA; GTACACCCCAACCCCCAACCTTATTGGAAAACAATGCCCAAGGGTTAGGCTTTGGAAGGG; CAAAATGGGCCCCTTTAAATTTCCTCCCAAGGACCAAGCCCTTTTAACCCTGGGAAATGT; CCCTAACTGGGGGGGGAAAGGGTGGCGATCAAGAATACATATGGGGAGTTTTTGGTAAAA; CCCCTCCCTTACACACCCCAGGGATGAAAGGCCCCCAAGTGGGAACCGGAACCATCAAAT; TCTTGCTGATATTAATTGGGCCCTAATTTCAAAAACAATGCTGTTTTACAAATTGGACCT; GACTTTAAGAACCAACCCAAAACCAAAGGAGTGAAATTTTTTGCAAAAAAAATTCCAATG; AAAAATCAGTACCCCTTGCCTTCGTTAAGTGCCCACCTCCCCTGGAATGATTTGTTTCCA; ACGGTGGTTCTCTCCAAATAATGGGGGGGGGTAACCCCCATTTTATGTCCACATTTGAGG; GTCCTTGCTATAACCGGGATAAACGGCTGGAGTTTGACACTCCTTGTACAAATCCCATTT; CTAAATTGGTGGCTCTCCGGAACACTTGTTTTTTCCCAAATTCTTGGCCGGGGACCTATT; TTTTTGCCCACCTTTTCTGTGCGGTGGAACGAAAAAAACCAGCCCAGGTTCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001001391
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001001391.1, NP_001001391.1
• RefSeq Size: 4605 bp
• RefSeq ORF: 1086 bp
• Locus ID: 960
• UniProt ID: P16070
• Cytogenetics: 11p13
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
• Protein Pathways: ECM-receptor interaction, Hematopoietic cell lineage
• Summary: The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (4) lacks multiple coding-exons compared to variant 1. The translation remains in-frame. The resulting isoform (4) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record represents the CD44*001.1.1 allele.