Reticulocalbin 3 (RCN3) (NM_020650) Human Untagged Clone

SKU SC128149

RCN3 (untagged)-Human reticulocalbin 3, EF-hand calcium binding domain (RCN3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Reticulocalbin 3
• Synonyms: RLP49
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020650, the custom clone sequence may differ by one or more nucleotides; ; ; ATGATGTGGCGACCATCAGTTCTGCTGCTTCTGTTGCTACTGAGGCACGGGGCCCAGGGGAAGCCATCCC; CAGACGCAGGCCCTCATGGCCAGGGGAGGGTGCACCAGGCGGCCCCCCTGAGCGACGCTCCCCATGATGA; CGCCCACGGGAACTTCCAGTACGACCATGAGGCTTTCCTGGGACGGGAAGTGGCCAAGGAATTCGACCAA; CTCACCCCAGAGGAAAGCCAGGCCCGTCTGGGGCGGATCGTGGACCGCATGGACCGCGCGGGGGACGGCG; ACGGCTGGGTGTCGCTGGCCGAGCTTCGCGCGTGGATCGCGCACACGCAGCAGCGGCACATACGGGACTC; GGTGAGCGCGGCCTGGGACACGTACGACACGGACCGCGACGGGCGTGTGGGTTGGGAGGAGCTGCGCAAC; GCCACCTATGGCCACTACGCGCCCGGTGAAGAATTTCATGACGTGGAGGATGCAGAGACCTACAAAAAGA; TGCTGGCTCGGGACGAGCGGCGTTTCCGGGTGGCCGACCAGGATGGGGACTCGATGGCCACTCGAGAGGA; GCTGACAGCCTTCCTGCACCCCGAGGAGTTCCCTCACATGCGGGACATCGTGATTGCTGAAACCCTGGAG; GACCTGGACAGAAACAAAGATGGCTATGTCCAGGTGGAGGAGTACATCGCGGATCTGTACTCAGCCGAGC; CTGGGGAGGAGGAGCCGGCGTGGGTGCAGACGGAGAGGCAGCAGTTCCGGGACTTCCGGGATCTGAACAA; GGATGGGCACCTGGATGGGAGTGAGGTGGGCCACTGGGTGCTGCCCCCTGCCCAGGACCAGCCCCTGGTG; GAAGCCAACCACCTGCTGCACGAGAGCGACACGGACAAGGATGGGCGGCTGAGCAAAGCGGAAATCCTGG; GTAATTGGAACATGTTTGTGGGCAGTCAGGCCACCAACTATGGCGAGGACCTGACCCGGCACCACGATGA; GCTGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020650 unedited; CCCCGTTCAAAATTTGTATACGACTCATATAGGCGGCCTGCGAATCGGCACCAGGNAACG; CAGAGCGGAGCGTGGAGAGCGGAGCGAAGCTGGATAACAGGGGACCGATGATGTGGCGAC; CATCAGTTCTGCTGCTTCTGTTGCTACTGAGGCACGGGGCCCAGGGGAAGCCATCCCCAG; ACGCAGGCCCTCATGGCCAGGGGAGGGTGCACCAGGCGGCCCCCCTGAGCGACGCTCCCC; ATGATGACGCCCACGGGAACTTCCAGTACGACCATGAGGCTTTCCTGGGACGGGAAGTGG; CCAAGGAATTCGACCAACTCACCCCAGAGGAAAGCCAGGCCCGTCTGGGGCGGATCGTGG; ACCGCATGGACCGCGCGGGGGACGGCGACGGCTGGGTGTCGCTGGCCGAGCTTCGCGCGT; GGATCGCGCACACGCAGCAGCGGCACATACGGGACTCGGTGAGCGCGGCCTGGGACACGT; ACGACACGGACCGCGACGGGCGTGTGGGTTGGGAGGAGCTGCGCAACGCCACCTATGGCC; ACTACGCGCCCGGTGAAGAATTTCATGACGTGGAGGATGCAGAGACCTACAAAAAGATGC; TGGCTCGGGACGAGCGGCGTTTCCGGGTGGCCGCCAGGATGGGGACTCGATGGCCACTCG; AGAGGAGCTGACAGCCTTCCTGCCCCCGNAGGAGTTCCCTCACATGCGGNGACATCGTGA; TTGCTGACACCCTGNAGGACCTGGACAGAACCAAGAGGGCTTGTCCAGGTGGGAGGAGTA; CATCGCGGACTGCGCCCCCACCCCCCCTGGGGGAGGAGAGCCGGCGTGGGTGCGACGGAG; GGCCACCAATTCTGGCTTTTCCGGATCTGACCACGATGGCACTGATTGNGAAAGTNAGGA; GCCCTCTTGGGTGCTGCCCCTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020650 unedited; NTTTCGTGCGGCTTTTATGCCTCTGGCANGAGGNAGTGTCCCCGGCCAGGCCAGGCAGGA; GAGGGCTGGGGCCAGGGGAGTTTCAGTCTATGTGGTGGAGGCTCAGATTTGGAGCTGGAG; GGGTTGGGGCGGTTCTTAGAGCTGAGCTTGGGGCCAAGGGTCCCTGGGTCTGGGCTGGGA; GACTCAGTCAGAAATAGGTATTGCTTATCCTCTGGGACTATGACAGCCCCTCCCTGGGGT; TGGGGGTGTGACAGGGACAGAAGCCGACCCAGGGGGTCCCTGACAGCCCAGGGGCAGGAG; GATGCCTGGGACTGCATCTGCCTCCTGCGGGGCCTGGACAGGGAGGGGGCCAGACCACAG; CGGCCCCTCCTCAGGTCATTGTGCGGCCCTCTGAGGCTGTGGCACGTGCGCGGTGCTCAC; AGCTCATCGCGCTGCCGGGTCAGGTCCTCGCCATAGTTGGTGGCCTGACTGCCCACAAAC; GTGGTCCAGTTACGCCAGATTTCCGCTTTGCTTAGCCGCCAATCCTTGTCCGTGTCCGCT; CCGTGCACCGGGTGGGTGGCTCCCACGAAGGGCTGGGCTTGGTCAGGGGGCAGGACTCAG; TGGTTCACCTTACTCCCATCCAGGTGCCTATCTTTGTTCCAATCCCGGGAATCTCGTAGT; TGCTGTCCCGTCGGTTGCGGGCATGCTGGCTCTTCGTCCGCAGGCTCGCTTGATTCTACT; TCCGAGGTGGTGCTCGTCAACCGGTCCTTAACGTTCTTCTCTCTCGGGCACGGACCCTAG; TGTTTTATCGACCACTACCTTCAGGTTTGTAGCGCGAACCCTTCCGCCGTGAGGGAAGGC; ATCCCCCTCCTCTCCAGAGAGGTTTATAATCCCCTTCCGGGGGGCCCTACCGGACAACCC; GTGTTGCCCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_020650
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020650.2, NP_065701.2
• RefSeq Size: 1882 bp
• RefSeq ORF: 987 bp
• Locus ID: 57333
• UniProt ID: Q96D15
• Cytogenetics: 19q13.33
• Domains: EFh
• Summary: Probable molecular chaperone assisting protein biosynthesis and transport in the endoplasmic reticulum (PubMed:16433634, PubMed:28939891). Required for the proper biosynthesis and transport of pulmonary surfactant-associated protein A/SP-A, pulmonary surfactant-associated protein D/SP-D and the lipid transporter ABCA3 (By similarity). By regulating both the proper expression and the degradation through the endoplasmic reticulum-associated protein degradation pathway of these proteins plays a crucial role in pulmonary surfactant homeostasis (By similarity). Has an anti-fibrotic activity by negatively regulating the secretion of type I and type III collagens (PubMed:28939891). This calcium-binding protein also transiently associates with immature PCSK6 and regulates its secretion (PubMed:16433634).[UniProtKB/Swiss-Prot Function]