DLK (DLK1) (NM_003836) Human Untagged Clone

CAT#: SC127962

DLK1 (untagged)-Human delta-like 1 homolog (Drosophila) (DLK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DLK
• Synonyms: Delta1; DLK; DLK-1; FA1; pG2; Pref-1; PREF1; ZOG
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003836, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCGCGACCGAAGCCCTCCTGCGCGTCCTCTTGCTCCTGCTGGCTTTCGGCCACAGCACCTATGGGG
  CTGAATGCTTCCCGGCCTGCAACCCCCAAAATGGATTCTGCGAGGATGACAATGTTTGCAGGTGCCAGCC
  TGGCTGGCAGGGTCCCCTTTGTGACCAGTGCGTGACCTCTCCCGGCTGCCTTCACGGACTCTGTGGAGAA
  CCCGGGCAGTGCATTTGCACCGACGGCTGGGACGGGGAGCTCTGTGATAGAGATGTTCGGGCCTGCTCCT
  CGGCCCCCTGTGCCAACAACGGGACCTGCGTGAGCCTGGACGATGGCCTCTATGAATGCTCCTGTGCCCC
  CGGGTACTCGGGAAAGGACTGCCAGAAAAAGGACGGGCCCTGTGTGATCAACGGCTCCCCCTGCCAGCAC
  GGAGGCACCTGCGTGGATGATGAGGGCCGGGCCTCCCATGCCTCCTGCCTGTGCCCCCCTGGCTTCTCAG
  GCAATTTCTGCGAGATCGTGGCCAACAGCTGCACCCCCAACCCATGCGAGAACGACGGCGTCTGCACTGA
  CATTGGGGGCGACTTCCGCTGCCGGTGCCCAGCCGGCTTCATCGACAAGACCTGCAGCCGCCCGGTGACC
  AACTGCGCCAGCAGCCCGTGCCAGAACGGGGGCACCTGCCTGCAGCACACCCAGGTGAGCTACGAGTGTC
  TGTGCAAGCCCGAGTTCACAGGTCTCACCTGTGTCAAGAAGCGCGCGCTGAGCCCCCAGCAGGTCACCCG
  TCTGCCCAGCGGCTATGGGCTGGCCTACCGCCTGACCCCTGGGGTGCACGAGCTGCCGGTGCAGCAGCCG
  GAGCACCGCATCCTGAAGGTGTCCATGAAAGAGCTCAACAAGAAAACCCCTCTCCTCACCGAGGGCCAGG
  CCATCTGCTTCACCATCCTGGGCGTGCTCACCAGCCTGGTGGTGCTGGGCACTGTGGGTATCGTCTTCCT
  CAACAAGTGCGAGACCTGGGTGTCCAACCTGCGCTACAACCACATGCTGCGGAAGAAGAAGAACCTGCTG
  CTTCAGTACAACAGCGGGGAGGACCTGGCCGTCAACATCATCTTCCCCGAGAAGATCGACATGACCACCT
  TCAGCAAGGAGGCCGGCGACGAGGAGATCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003836 unedited
  NGGTTGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCGGTGGAGA
  GCGCAGCGCGCAGCCCGGTGCAGCCCTGGCTTTCCCCTCGCTGCGCGCCCGCGCCCCCTT
  TCGCGTCCGCAACCAGAAGCCCAGTGCGGCGCCAGGAGCCGGACCCGCGCCCGCACCGCT
  CCCGGGACCGCGACCCCGGCCGCCCAGAGATGACCGCGACCGAAGCCCTCCTGCGCGTCC
  TCTTGCTCCTGCTGGCTTTCGGCCACAGCACCTATGGGGCTGAATGCTTCCCGGCCTGCA
  ACCCCCAAAATGGATTCTGCGAGGATGACAATGTTTGCAGGTGCCAGCCTGGCTGGCAGG
  GTCCCCTTTGTGACCAGTGCGTGACCTCTCCCGGCTGCCTTCACGGACTCTGTGGAGAAC
  CCGGGCAGTGCATTTGCACCGACGGCTGGGACGGGGAGCTCTGTGATAGAGATGTTCGGG
  CCTGCTCCTCGGCCCCCTGTGCCAACAACGGGACCTGCGTGAGCCTGGACGATGGCCTCT
  ATGAATGCTCCTGTGCCCCCGGGTACTCGGGAAAGGACTGCCAGAAAAAGGACGGGCCCT
  GTGTGATCAACGGCTCCCCCTGCCAGCACGGAGGCACCTGCGTGGATGATGAGGGCCGGG
  CCTCCCATGCCTCCTGCCTGTGCCCCCCTGGCTTCTCAGGCAATTTCTGCGAGATCGTGG
  CCAACAGCTGCACCCCCAACCCATGCGAGAACGACNGGCGTCTGCACTGACATCGNGGGC
  GACTTCCGCTGCCGGTGCCCAGCCGGCTTTCATCGACAGACCTGCAGNNCGNCCGGTGAC
  CAACTGCGCCAGCAGCCCGTGCCAGAACGGGGGCACCTGGCTGCAGCACACCAGTGAGCT
  ACGAGTGTCTGTGCAAGCCCGATTNACAGGTCTNACCTGTGTCANGAGCGCGCGCTGAGC
  CCCAGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_003836 unedited
  NGGGCCTTTTCGATGGAATTCTTCATATTATTCTGTATCATGCATTAGNGNANAGAAGNG
  GATTGTTTTTGCATTGCGTTTGTCACACAGCAGCACAAAGACAATATATGTAAGCGTAGC
  GTTCACCAGATTTGACACAAGAGATAGCGAACACCACAAAGATTAGGACAGACCGCGTGT
  AGTAAGCTCTGCGGAACTCCAAGAATCTAGAGGGGGCTGTGGGAACGCTGCTTAGATCTC
  CTCGTCGCCGGCCTCCTTGCTGAAGGTGGTCATGTCGATCTTCTCGGGGAAGATGATGTT
  GACGGCCAGGTCCTCCCCGCTGTTGTACTGAAGCAGCAGGTTCTTCTTCTTCCGCAGCAT
  GTGGTTGTAGCGCAGGTTGGACACCCAGGTCTCGCACTTGTTGAGGAAGACGATACCCAC
  AGTGCCCAGCACCACCAGGCTGGTGAGCACGCCCAGGATGGTGAAGCAGATGGCCTGGCC
  CTCGGTGAGGAGAGGGGTTTTCTTGTTGAGCTCTTTCATGGACACCTTCAGGATGCGGTG
  CTCCGGCTGCTGCACCGGCAGCTCGTGCACCCCAGGGGTCAGGCGGTAGGCCAGCCCATA
  GCCGCTGGGCAGACGGGTGACCTGCTGGGGGCTCAGCGCGCGCTTCTTGACACAGGTGAG
  ACCTGTGAACTCGGGCTTGCACAGACACTCGTAGCTCACCTGGGTGTGCTGCAGGCAGGT
  GCCCCCGTTCTGGCACGGGCTGCTGGCGCAGTTGGTCACCGGGCGGCTGCAGGTCTTGTC
  GATGAAGCCGGCTGGGCACCGGGCAGCGGAAGTCGCCCCCGATGTCAGTGCAGACGCCGT
  CGTTCTCGCATGGGTTGGGGGTGCAGCTGGTTGGCCCGATCTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003836
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003836.4, NP_003827.3
• RefSeq Size: 1532 bp
• RefSeq ORF: 1152 bp
• Locus ID: 8788
• UniProt ID: P80370
• Cytogenetics: 14q32.2
• Domains: EGF_CA, EGF, EGF
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Gene Summary: This gene encodes a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.