DLK (DLK1) (NM_003836) Human Untagged Clone

CAT#: SC127962

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DLK1 (untagged)-Human delta-like 1 homolog (Drosophila) (DLK1)



  "NM_003836" in other vectors (7)

Reconstitution Protocol

USD 686.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DLK1
Synonyms Delta1; DLK; DLK-1; FA1; pG2; Pref-1; PREF1; ZOG
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_003836, the custom clone sequence may differ by one or more nucleotides


ATGACCGCGACCGAAGCCCTCCTGCGCGTCCTCTTGCTCCTGCTGGCTTTCGGCCACAGCACCTATGGGG
CTGAATGCTTCCCGGCCTGCAACCCCCAAAATGGATTCTGCGAGGATGACAATGTTTGCAGGTGCCAGCC
TGGCTGGCAGGGTCCCCTTTGTGACCAGTGCGTGACCTCTCCCGGCTGCCTTCACGGACTCTGTGGAGAA
CCCGGGCAGTGCATTTGCACCGACGGCTGGGACGGGGAGCTCTGTGATAGAGATGTTCGGGCCTGCTCCT
CGGCCCCCTGTGCCAACAACGGGACCTGCGTGAGCCTGGACGATGGCCTCTATGAATGCTCCTGTGCCCC
CGGGTACTCGGGAAAGGACTGCCAGAAAAAGGACGGGCCCTGTGTGATCAACGGCTCCCCCTGCCAGCAC
GGAGGCACCTGCGTGGATGATGAGGGCCGGGCCTCCCATGCCTCCTGCCTGTGCCCCCCTGGCTTCTCAG
GCAATTTCTGCGAGATCGTGGCCAACAGCTGCACCCCCAACCCATGCGAGAACGACGGCGTCTGCACTGA
CATTGGGGGCGACTTCCGCTGCCGGTGCCCAGCCGGCTTCATCGACAAGACCTGCAGCCGCCCGGTGACC
AACTGCGCCAGCAGCCCGTGCCAGAACGGGGGCACCTGCCTGCAGCACACCCAGGTGAGCTACGAGTGTC
TGTGCAAGCCCGAGTTCACAGGTCTCACCTGTGTCAAGAAGCGCGCGCTGAGCCCCCAGCAGGTCACCCG
TCTGCCCAGCGGCTATGGGCTGGCCTACCGCCTGACCCCTGGGGTGCACGAGCTGCCGGTGCAGCAGCCG
GAGCACCGCATCCTGAAGGTGTCCATGAAAGAGCTCAACAAGAAAACCCCTCTCCTCACCGAGGGCCAGG
CCATCTGCTTCACCATCCTGGGCGTGCTCACCAGCCTGGTGGTGCTGGGCACTGTGGGTATCGTCTTCCT
CAACAAGTGCGAGACCTGGGTGTCCAACCTGCGCTACAACCACATGCTGCGGAAGAAGAAGAACCTGCTG
CTTCAGTACAACAGCGGGGAGGACCTGGCCGTCAACATCATCTTCCCCGAGAAGATCGACATGACCACCT
TCAGCAAGGAGGCCGGCGACGAGGAGATCTAA


>OriGene 5' read for NM_003836 unedited
NGGTTGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCGGTGGAGA
GCGCAGCGCGCAGCCCGGTGCAGCCCTGGCTTTCCCCTCGCTGCGCGCCCGCGCCCCCTT
TCGCGTCCGCAACCAGAAGCCCAGTGCGGCGCCAGGAGCCGGACCCGCGCCCGCACCGCT
CCCGGGACCGCGACCCCGGCCGCCCAGAGATGACCGCGACCGAAGCCCTCCTGCGCGTCC
TCTTGCTCCTGCTGGCTTTCGGCCACAGCACCTATGGGGCTGAATGCTTCCCGGCCTGCA
ACCCCCAAAATGGATTCTGCGAGGATGACAATGTTTGCAGGTGCCAGCCTGGCTGGCAGG
GTCCCCTTTGTGACCAGTGCGTGACCTCTCCCGGCTGCCTTCACGGACTCTGTGGAGAAC
CCGGGCAGTGCATTTGCACCGACGGCTGGGACGGGGAGCTCTGTGATAGAGATGTTCGGG
CCTGCTCCTCGGCCCCCTGTGCCAACAACGGGACCTGCGTGAGCCTGGACGATGGCCTCT
ATGAATGCTCCTGTGCCCCCGGGTACTCGGGAAAGGACTGCCAGAAAAAGGACGGGCCCT
GTGTGATCAACGGCTCCCCCTGCCAGCACGGAGGCACCTGCGTGGATGATGAGGGCCGGG
CCTCCCATGCCTCCTGCCTGTGCCCCCCTGGCTTCTCAGGCAATTTCTGCGAGATCGTGG
CCAACAGCTGCACCCCCAACCCATGCGAGAACGACNGGCGTCTGCACTGACATCGNGGGC
GACTTCCGCTGCCGGTGCCCAGCCGGCTTTCATCGACAGACCTGCAGNNCGNCCGGTGAC
CAACTGCGCCAGCAGCCCGTGCCAGAACGGGGGCACCTGGCTGCAGCACACCAGTGAGCT
ACGAGTGTCTGTGCAAGCCCGATTNACAGGTCTNACCTGTGTCANGAGCGCGCGCTGAGC
CCCAGCAG
>OriGene 3' genomic read for NM_003836 unedited
NGGGCCTTTTCGATGGAATTCTTCATATTATTCTGTATCATGCATTAGNGNANAGAAGNG
GATTGTTTTTGCATTGCGTTTGTCACACAGCAGCACAAAGACAATATATGTAAGCGTAGC
GTTCACCAGATTTGACACAAGAGATAGCGAACACCACAAAGATTAGGACAGACCGCGTGT
AGTAAGCTCTGCGGAACTCCAAGAATCTAGAGGGGGCTGTGGGAACGCTGCTTAGATCTC
CTCGTCGCCGGCCTCCTTGCTGAAGGTGGTCATGTCGATCTTCTCGGGGAAGATGATGTT
GACGGCCAGGTCCTCCCCGCTGTTGTACTGAAGCAGCAGGTTCTTCTTCTTCCGCAGCAT
GTGGTTGTAGCGCAGGTTGGACACCCAGGTCTCGCACTTGTTGAGGAAGACGATACCCAC
AGTGCCCAGCACCACCAGGCTGGTGAGCACGCCCAGGATGGTGAAGCAGATGGCCTGGCC
CTCGGTGAGGAGAGGGGTTTTCTTGTTGAGCTCTTTCATGGACACCTTCAGGATGCGGTG
CTCCGGCTGCTGCACCGGCAGCTCGTGCACCCCAGGGGTCAGGCGGTAGGCCAGCCCATA
GCCGCTGGGCAGACGGGTGACCTGCTGGGGGCTCAGCGCGCGCTTCTTGACACAGGTGAG
ACCTGTGAACTCGGGCTTGCACAGACACTCGTAGCTCACCTGGGTGTGCTGCAGGCAGGT
GCCCCCGTTCTGGCACGGGCTGCTGGCGCAGTTGGTCACCGGGCGGCTGCAGGTCTTGTC
GATGAAGCCGGCTGGGCACCGGGCAGCGGAAGTCGCCCCCGATGTCAGTGCAGACGCCGT
CGTTCTCGCATGGGTTGGGGGTGCAGCTGGTTGGCCCGATCTCA
Restriction Sites NotI-NotI     
ACCN NM_003836
Insert Size 1700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003836.4, NP_003827.3
RefSeq Size 1532 bp
RefSeq ORF 1152 bp
Locus ID 8788
UniProt ID P80370
Cytogenetics 14q32.2
Domains EGF_CA, EGF, EGF
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Gene Summary This gene encodes a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal. [provided by RefSeq, Nov 2015]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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