LRRC8A (NM_019594) Human Untagged Clone

SKU SC127734

LRRC8A (untagged)-Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: LRRC8A
• Synonyms: AGM5; HsLRRC8A; LRRC8; SWELL1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127734 sequence for NM_019594 edited (data generated by NextGen Sequencing); ATGATTCCGGTGACAGAGCTCCGCTACTTTGCGGACACGCAGCCAGCATACCGGATCCTG; AAGCCGTGGTGGGATGTGTTCACAGACTACATCTCTATCGTCATGCTGATGATTGCCGTC; TTCGGGGGGACGCTGCAGGTCACCCAAGACAAGATGATCTGCCTGCCTTGTAAGTGGGTC; ACCAAGGACTCCTGCAATGATTCGTTCCGGGGCTGGGCAGCCCCTGGCCCGGAGCCCACC; TACCCCAACTCCACCATTCTGCCGACCCCTGACACGGGCCCCACAGGCATCAAGTATGAC; CTGGACCGGCACCAGTACAACTACGTGGACGCTGTGTGCTATGAGAACCGACTGCACTGG; TTTGCCAAGTACTTCCCCTACCTGGTGCTTCTGCACACGCTCATCTTCCTGGCCTGCAGC; AACTTCTGGTTCAAATTCCCGCGCACCAGCTCGAAGCTGGAGCACTTTGTGTCTATCCTG; CTGAAGTGCTTCGACTCGCCCTGGACCACGAGGGCCCTGTCGGAGACAGTGGTGGAGGAG; AGCGACCCCAAGCCGGCCTTCAGCAAGATGAATGGGTCCATGGACAAAAAGTCATCGACC; GTCAGTGAGGACGTGGAGGCCACCGTGCCCATGCTGCAGCGGACCAAGTCACGGATCGAG; CAGGGTATCGTGGACCGCTCAGAGACGGGCGTGCTGGACAAGAAGGAGGGGGAGCAAGCC; AAGGCGCTGTTTGAGAAGGTGAAGAAGTTCCGGACCCATGTGGAGGAGGGGGACATTGTG; TACCGCCTCTACATGCGGCAGACCATCATCAAGGTGATCAAGTTCATCCTCATCATCTGC; TACACCGTCTACTACGTGCACAACATCAAGTTCGACGTGGACTGCACCGTGGACATTGAG; AGCCTGACGGGCTACCGCACCTACCGCTGTGCCCACCCCCTGGCCACACTCTTCAAGATC; CTGGCGTCCTTCTACATCAGCCTAGTCATCTTCTACGGCCTCATCTGCATGTACACACTG; TGGTGGATGCTACGGCGCTCCCTCAAGAAGTACTCGTTTGAGTCGATCCGTGAGGAGAGC; AGCTACAGCGACATCCCCGACGTCAAGAACGACTTCGCCTTCATGCTGCACCTCATTGAC; CAATACGACCCGCTCTACTCCAAGCGCTTCGCCGTCTTCCTGTCGGAGGTGAGTGAGAAC; AAGCTGCGGCAGCTGAACCTCAACAACGAGTGGACGCTGGACAAGCTCCGGCAGCGGCTC; ACCAAGAACGCGCAGGACAAGCTGGAGCTGCACCTGTTCATGCTCAGTGGCATCCCTGAC; ACTGTGTTTGACCTGGTGGAGCTGGAGGTCCTCAAGCTGGAGCTGATCCCCGACGTGACC; ATCCCGCCCAGCATTGCCCAGCTCACGGGCCTCAAGGAGCTGTGGCTCTACCACACAGCG; GCCAAGATTGAAGCGCCCGCGCTGGCCTTCCTGCGCGAGAACCTGCGGGCGCTGCACATC; AAGTTCACTGACATCAAGGAGATCCCGCTGTGGATCTATAGCCTGAAGACACTGGAGGAG; CTGCACCTGACGGGCAACCTGAGCGCGGAGAACAACCGCTACATCGTCATCGACGGGCTG; CGGGAGCTCAAACGCCTCAAGGTGCTGCGGCTCAAGAGCAACCTAAGCAAGCTGCCACAG; GTGGTCACAGATGTGGGCGTGCACCTGCAGAAGCTGTCCATCAACAATGAGGGCACCAAG; CTCATCGTCCTCAACAGCCTCAAGAAGATGGCGAACCTGACTGAGCTGGAGCTGATCCGC; TGTGACCTGGAGCGCATCCCCCACTCCATCTTCAGCCTCCACAACCTGCAGGAGATTGAC; CTCAAGGACAACAACCTCAAGACCATCGAGGAGATCATCAGCTTCCAGCACCTGCACCGC; CTCACCTGCCTTAAGCTGTGGTACAACCACATCGCCTACATCCCCATCCAGATCGGCAAC; CTCACCAACCTGGAGCGCCTCTACCTGAACCGCAACAAGATCGAGAAGATCCCCACCCAG; CTCTTCTACTGCCGCAAGCTGCGCTACCTGGACCTCAGCCACAACAACCTGACCTTCCTC; CCTGCCGACATCGGCCTCCTGCAGAACCTCCAGAACCTAGCCATCACGGCCAACCGGATC; GAGACGCTCCCTCCGGAGCTCTTCCAGTGCCGGAAGCTGCGGGCCCTGCACCTGGGCAAC; AACGTGCTGCAGTCACTGCCCTCCAGGGTGGGCGAGCTGACCAACCTGACGCAGATCGAG; CTGCGGGGCAACCGGCTGGAGTGCCTGCCTGTGGAGCTGGGCGAGTGCCCACTGCTCAAG; CGCAGCGGCTTGGTGGTGGAGGAGGACCTGTTCAACACACTGCCACCCGAGGTGAAGGAG; CGGCTGTGGAGGGCTGACAAGGAGCAGGCCTGA; ; Clone variation with respect to NM_019594.3; 1014 t=>c;1509 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019594 unedited; ACTCACTATAGGGGCGGCCGCGAATCGGTACGAGGCTGCGGCCGGCGGCGGGACGGAGCG; GCCGGGGCCTGGGGCTGCCTGCCGGGCGGCCGGGCGCGGCGAGCCCAGGGAGGCAGCGTC; CATGGAGCAAAAGGAATGCCAGGATCCTGCACAGGCAGACGCGGGCCAGCCTCAGCACCG; ACAGCCGACGCGCAGATAGCAGAGCCATCCTTGGGGTTGAACCATGATTCCGGTGACAGA; GCTCCGCTACTTTGCGGACACGCAGCCAGCATACCGGATCCTGAAGCCGTGGTGGGATGT; GTTCACAGACTACATCTCTATCGTCATGCTGATGATTGCCGTCTTCGGGGGGACGCTGCA; GGTCACCCAAGACAAGATGATCTGCCTGCCTTGTAAGTGGGTCACCAAGGACTCCTGCAA; TGATTCGTTCCGGGGCTGGGCAGCCCCTGGCCCGGAGCCCACCTACCCCAACTCCACCAT; TCTGCCGACCCCTGACACGGGCCCCACAGGCATCAAGTATGACCTGGACCGGCACCAGTA; CAACTACGTGGACGCTGTGTGCTATGAGAACCGACTGCACTGGTTTGCCAAGTACTTCCC; CTACCTGGTGCTTCTGCACACGCTCATCTTCCTGGCCTGCAGCAACTTCTGGTTCAAATT; CCCGCGCACCAGCTCGAAGCTGGAGCACTTTGTGTCTATCCTGCTGAAGTGCTTCGACTC; GCCCTGGGACCACGAGGCCCTGTCGGAGACAGTGGTGGNAGAGAGCGACCCCAAGCCGGC; CTTCAGCAGATGAATGGGTCCATGGGACAAAGTCATCGACCGGTCAGTGAGACGTGGAGG; CACCGTNGCCATGCTGCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019594 unedited; TCTATGGACGCGGCCGCTTTCTANAGTCGAGTTTTTTTTTTTTTTTTTTAATACCCAAAC; CATCCATTTTCAAAGCTTTTTTTTTAAAAAAATGTTTTTTGATTAAAAAAAAAAAAACAA; AAAACTAAAACTGCCACAAATACTGAAGAGGCAAGCTCCAGGCCTGGCCTTTCCAGCTGG; AGGCCTGCTCTCCCGTGGACCGCACCGCAGAGCCCAGCCCGGCACTGCTGGGTGACCGTC; CCATGGCTGGTTCGCCCCGGACCACTCTGCTGAAGTCCAGGTGGGTGTTAAATGCCCGGC; GGCATCGCTAAATAAGGACAAGGGGAAAAGGCAGCCCACCTTTCCCTGAACACCTTCCAT; CTGGGCTGAACTTTCTTGGGAATGTATAAGTTATCTGGATGAAGGAGGCGGGAGATGGAG; AAAAATAAATACCAAGAACTAAAGTGAGGGTCCCAGGACCTCTGGTGGCAGCTCAGCCCC; TGGGGCAGAGCTGGCCTCCAGGGAGGAGACCCTGATTATCCAAATACTGCGCTCTTGAGA; CTTGCTCTCCTCCACAAGCACTTGCCCTGGGGGACGTGAGTCTCAGAGGGAGAAAAGGGG; CCAGCCCCACAGATACTGTCCTCTCGCTCTGGCCTGACTCACAAGCGGCCCCAGGCTCCT; GCCCAGCCACGAGCTGTCCTGCCTGTCCGGGAGTTCTGGGAGAACCTAGGCCTGCCCCTC; CGGCCCTGAGGACCTGGCAGCGGTCCTGCTGCTTGCTGTGCTGGGCCCGCCTCGCTCAAC; CCTGCTCCTTTGTAGCCCCTCCAAGCCGGTCTCTCACCTNGGGTGGAAGGGTGTGAACAG; TCCCTCTACACCACAAACCGNTGNGCTTGACCAGGGGNACTCCCCAGCTTCAAGGCAGAA; CTCCACCCGNTGCCCCCACTTAACTGGTCAGGTGCACACC;
• Restriction Sites: NotI-NotI
• ACCN: NM_019594
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_019594.2, NP_062540.2
• RefSeq Size: 4296 bp
• RefSeq ORF: 2433 bp
• Locus ID: 56262
• UniProt ID: Q8IWT6
• Cytogenetics: 9q34.11
• Protein Families: Transmembrane
• Summary: This gene encodes a protein belonging to the leucine-rich repeat family of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions. This family member is a putative four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All variants (1-3) encode the same protein.