Caldesmon (CALD1) (NM_033157) Human Untagged Clone

SKU SC127484

CALD1 (untagged)-Human caldesmon 1 (CALD1), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: Caldesmon
• Synonyms: CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033157, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGATGATTTTGAGCGTCGCAGAGAACTTAGAAGGCAAAAGAGGGAGGAGATGCGACTCGAAGCAGAAA; GAATCGCCTACCAGAGGAATGACGATGATGAAGAGGAGGCAGCCCGGGAACGGCGCCGCCGAGCCCGACA; GGAACGGCTGCGGCAGAAGCAGGAGGAAGAATCCTTGGGACAGGTGACCGACCAGGTGGAGGTGAATGCC; CAGAACAGTGTGCCTGACGAGGAGGCCAAGACAACCACCACAAACACTCAAGTGGAAGGGGATGATGAGG; CCGCATTCCTGGAGCGCCTGGCTCGGCGTGAGGAAAGACGCCAAAAACGCCTTCAGGAGGCTCTGGAGCG; GCAGAAGGAGTTCGACCCAACAATAACAGATGCAAGTCTGTCGCTCCCAAGCAGAAGAATGCAAAATGAC; ACAGCAGAAAATGAAACTACCGAGAAGGAAGAAAAAAGTGAAAGTCGCCAAGAAAGATACGAGATAGAGG; AAACAGAAACAGTCACCAAGTCCTACCAGAAGAATGATTGGAGGGATGCTGAAGAAAACAAGAAAGAAGA; CAAGGAAAAGGAGGAGGAGGAAGAGGAGAAGCCAAAGCGAGGGAGCATTGGAGAAAATCAGGGAGAAGAG; AAGGGAACTAAAGTGCAAGCTAAAAGAGAAAAGCTCCAAGAAGACAAGCCTACCTTCAAAAAAGAAGAGA; TCAAAGATGAAAAGATTAAAAAGGACAAAGAACCCAAAGAAGAAGTTAAGAGCTTCATGGATCGAAAGAA; GGGATTTACAGAAGTTAAGTCGCAGAATGGAGAATTCATGACCCACAAACTTAAACATACTGAGAATACT; TTCAGCCGCCCTGGAGGGAGGGCCAGCGTGGACACCAAGGAGGCTGAGGGCGCCCCCCAGGTGGAAGCCG; GCAAAAGGCTGGAGGAGCTTCGTCGTCGTCGCGGGGAGACCGAGAGCGAAGAGTTCGAGAAGCTCAAACA; GAAGCAGCAGGAGGCGGCTTTGGAGCTGGAGGAACTCAAGAAAAAGAGGGAGGAGAGAAGGAAGGTCCTG; GAGGAGGAAGAGCAGAGGAGGAAGCAGGAGGAAGCCGATCGAAAACTCAGAGAGGAGGAAGAGAAGAGGA; GGCTAAAGGAAGAGATTGAAAGGCGAAGAGCAGAAGCTGCTGAGAAACGCCAGAAGATGCCAGAAGATGG; CTTGTCAGATGACAAGAAACCATTCAAGTGTTTCACTCCTAAAGGTTCATCTCTCAAGATAGAAGAGCGA; GCAGAATTTTTGAATAAGTCTGTGCAGAAAAGTGGTGTCAAATCGACCCATCAAGCAGCAATAGTCTCCA; AGATTGACAGCAGACTGGAGCAGTATACCAGTGCAATTGAGGGAACAAAAAGCGCAAAACCTACAAAGCC; GGCAGCCTCGGATCTTCCTGTTCCTGCTGAAGGTGTACGCAACATCAAGAGTATGTGGGAGAAAGGGAAT; GTGTTTTCATCCCCCACTGCAGCAGGCACACCAAATAAGGAAACTGCTGGCTTGAAGGTAGGGGTTTCTA; GCCGCATCAATGAATGGCTAACTAAAACCCCAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTT; GAGACCAGGAGACGTATCCAGCAAGCGGAACCTCTGGGAAAAGCAATCTGTGGATAAGGTCACTTCCCCC; ACTAAGGTTTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033157 unedited; GGCACGAGCGCCTGCTTGCTCTCTGGCTGTGCTCCTGCTTAAAGAAATCAGTCCTTCCTT; TCCGACTTAGTCCTCGGGAAGAAGTTTCAGACTACAAGGTATCATTGGAACATTTCAAGA; TCATCAAATCAAATTCCACAGGGATTGGTGACCAACCAGAAGGCTCAGACATCTGATTGC; TGACCTGTCCAGACATCATCTGGTCTCCCTGAACCTGAAATCACACCATGGATGATTTTG; AGCGTCGCAGAGAACTTAGAAGGCAAAAGAGGGAGGAGATGCGACTCGAAGCAGAAAGAA; TCGCCTACCAGAGGAATGACGATGATGAAGAGGAGGCAGCCCGGGAACGGCGCCGCCGAG; CCCGACAGGAACGGCTGCGGCAGAAGCAGGAGGAAGAATCCTTGGGACAGGTGACCGACC; AGGTGGAGGTGAATGCCCAGAACAGTGTGCCTGACGAGGAGGCCAAGACAACCACCACAA; ACACTCAAGTGGAAGGGGATGATGAGGCCGCATTCCTGGAGCGCCTGGCTCGGCGTGAGG; AAAGACGCCAAAAACGCCTTCAGGAGGCTCTGGAGCGGCAGAAGGAGTTCGACCCAACAA; TAACAGATGCCAGTCTGTCGCTCCCAAGCAGAAGAATGCAAAATGACACAGCAGAAAATG; AAACTACCGAGAACGAAGAAAAAAGTGACAGTCGCCCAGGAAAGATCCGAGATAGAGGAA; ACAGAAACAGTCACCAGGTCCTACANAANAATGATTGGAGGGATGCTTGAGAAACCGGAA; GAGGACAGGGAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033157 unedited; TTAGCTCTGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCAGGA; AGGAATTCCATTTATTGTGGATGCATTTTCACAATATATGTTTATTGGAGCGATCCATTA; TCAGTGAAAAGTATCAAGTGTTTATAAAATTTTTAGGAATGGCAGATTCACAGAACATGC; TAGTCAGCTTGCAGTTTTACCTCGTAAAGATAACAGAGAATTATAGTCAAACCAGTAAAC; AAGGAATTTACTTTTCAAAAGATTAAATCCAAACTGAACAAAATTCTACCCTAAAACTTA; CTCCATCCAAATATTGGAATAAAAGTCAGCAGTGACACATTCTCTTGTGAACTTTAGATT; TTTTAGAAAAATATGTAATAGTGATCAGGAGGCGCTCTTGTTCAAAAGTACAACAAAGCA; ATGTTACCTTACCATAGGCCTTAATTCAAACTTTGATCCATTCCACTCCAATGACGGGAG; CCAATGCTACCTGGGACACTTGTATTTGCAAATTCTGATTCACCTTATTGTACACTTGTG; CCTACTTTGCCACGAGGGCCCGCCTTCTGCACCCTTTTTGGCTTTCTTTCTTTTGCCTTC; AGTCTGCCAAAGCCCTAACACTCCCCGCTCTTCTTACAACTCATCAGGCCGCTTTATTTT; GTTCCGCATATGTTCCTCCCCATGACCTTCCTGCGCTGTTTCTTTTCCTTTGCCCCCCCC; TTCGCTTTCCACACCCCCTTTTTCGCCGGTCTCCCCTTTTGCTCCCCCCTCTTTCTACCC; ACCCCCTTCCTTTCTTTCTTCCTCTCGCCCTCTCTTTTCCCCTCCCCCCGGTTTTCCTCC; TCCTCTCCCCCCACTCTCTTTTCTCCTCCCTTTCGCCCGCACCCCCTCCCCCCCCGTCTC; ACCTACTCTCGCCCTCCCTCTCCCCACGTTGTCTCGTTCCTCTCCCCCTATCCCCTCCAC; CCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_033157
• Insert Size: 4470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_033157.2, NP_149347.1
• RefSeq Size: 4546 bp
• RefSeq ORF: 1695 bp
• Locus ID: 800
• UniProt ID: Q05682
• Cytogenetics: 7q33
• Domains: Caldesmon
• Protein Pathways: Vascular smooth muscle contraction
• Summary: This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the central coding region, compared to variant 1. It is mainly expressed in non-muscle tissues or cells. The resulting isoform (3, also known as WI-38 l-CaD I) is shorter than isoform 1.