ZNF521 (NM_015461) Human Untagged Clone

SKU SC127365

ZNF521 (untagged)-Human zinc finger protein 521 (ZNF521)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ZNF521
• Synonyms: EHZF; Evi3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127365 sequence for NM_015461 edited (data generated by NextGen Sequencing); ATGTCTCGCCGCAAGCAAGCGAAACCGAGATCCCTCAAAGACCCCAACTGTAAACTTGAA; GACAAGACTGAAGATGGAGAGGCACTAGATTGTAAGAAGAGGCCGGAAGACGGGGAGGAG; TTGGAAGACGAAGCTGTGCACAGCTGTGACAGCTGCCTCCAGGTGTTTGAATCGCTGAGC; GATATCACAGAACACAAGATTAATCAATGTCAACTGACAGATGGAGTGGATGTTGAAGAT; GATCCGACTTGCTCTTGGCCAGCTTCCTCACCTTCTAGCAAGGATCAGACTTCCCCTAGC; CATGGAGAAGGTTGCGATTTTGGAGAGGAAGAAGGTGGCCCTGGGCTTCCATACCCGTGT; CAATTCTGTGACAAGTCGTTTAGCCGCCTCAGCTACCTAAAGCACCATGAGCAGAGTCAC; AGTGACAAACTGCCTTTCAAATGCACCTACTGCAGTAGGCTGTTCAAACACAAGCGCAGC; CGAGATCGCCACATAAAACTCCACACCGGGGACAAGAAGTACCACTGCAGTGAATGTGAT; GCTGCGTTTTCCAGAAGTGATCACTTGAAGATCCACTTAAAGACTCACACGTCCAACAAG; CCATATAAATGTGCCATTTGTCGCCGTGGGTTTCTGTCCTCTAGTTCCTTACACGGACAC; ATGCAGGTTCATGAGAGGAACAAGGACGGCTCTCAGTCCGGTTCCAGGATGGAGGACTGG; AAGATGAAGGACACTCAGAAGTGCAGTCAGTGTGAGGAAGGCTTTGACTTCCCGGAAGAC; CTCCAAAAACACATTGCAGAGTGCCACCCCGAATGCTCCCCAAATGAGGACCGAGCGGCC; CTCCAGTGTGTCTACTGCCACGAGCTCTTCGTAGAGGAGACCTCCCTCATGAACCACATG; GAGCAGGTGCATAGCGGGGAGAAGAAGAACTCATGCAGCATTTGTTCTGAGAGTTTCCAC; ACAGTTGAGGAACTGTACAGCCACATGGACAGTCACCAGCAACCGGAGTCATGCAATCAC; AGCAACAGCCCTTCCCTGGTCACGGTGGGCTATACCTCCGTGTCCAGTACGACTCCAGAT; TCCAACCTCTCAGTGGACAGCTCAACCATGGTGGAAGCTGCCCCGCCAATCCCAAAGAGT; CGAGGGAGGAAGAGGGCCGCTCAACAAACCCCTGACATGACTGGTCCCTCGAGTAAACAA; GCAAAAGTTACCTACAGCTGTATTTACTGCAACAAACAATTATTTTCAAGTCTTGCAGTT; CTGCAGATTCACCTGAAAACTATGCACTTAGATAAGCCAGAACAGGCCCATATTTGTCAG; TATTGCTTGGAGGTCCTGCCCTCACTCTATAACCTAAATGAACATCTTAAGCAAGTGCAT; GAAGCTCAGGACCCAGGTCTGATTGTTTCTGCCATGCCTGCCATTGTCTACCAGTGTAAC; TTCTGTTCCGAAGTTGTCAACGACCTCAACACTCTTCAGGAACACATCCGATGTTCTCAT; GGATTTGCAAACCCTGCAGCTAAAGATAGTAATGCATTCTTTTGTCCCCATTGCTATATG; GGGTTTCTCACTGACTCTTCCCTGGAAGAGCATATTAGACAGGTTCATTGTGACCTCAGT; GGCTCCCGATTTGGGTCTCCAGTGCTTGGGACTCCCAAAGAACCAGTAGTAGAAGTCTAT; TCTTGTTCCTATTGTACAAATTCGCCAATATTCAACAGCGTTCTTAAACTGAACAAGCAT; ATCAAAGAGAATCATAAAAACATTCCCTTGGCCCTGAATTATATCCACAATGGGAAGAAA; TCCAGGGCCTTAAGCCCCCTATCTCCTGTGGCCATAGAGCAGACATCTCTTAAGATGATG; CAGGCAGTAGGAGGTGCACCTGCACGTCCCACTGGAGAATATATCTGTAATCAATGTGGT; GCTAAGTACACATCCCTAGACAGCTTTCAGACTCACCTAAAAACTCATCTCGACACTGTG; CTTCCAAAATTGACCTGTCCTCAGTGCAACAAGGAATTCCCCAACCAAGAATCCTTGCTG; AAGCATGTTACCATTCACTTTATGATCACTTCAACGTATTACATCTGTGAGAGTTGTGAC; AAGCAATTCACATCAGTGGATGACCTTCAGAAACACCTGCTGGACATGCACACCTTTGTC; TTCTTTCGCTGCACCCTCTGCCAGGAAGTTTTTGACTCAAAAGTCTCCATTCAGCTCCAC; TTGGCTGTGAAGCACAGTAACGAAAAGAAAGTCTATAGGTGCACATCTTGCAACTGGGAC; TTCCGCAACGAAACTGACTTGCAGCTCCATGTGAAACACAACCACCTGGAAAACCAAGGG; AAAGTGCATAAGTGCATTTTCTGCGGTGAGTCCTTTGGCACCGAGGTGGAGCTGCAATGC; CACATCACCACTCACAGTAAGAAGTACAACTGCAAGTTCTGTAGCAAAGCCTTCCATGCG; ATCATTTTGTTAGAAAAACACTTGCGAGAAAAACACTGTGTATTCGAAACCAAGACACCC; AACTGTGGAACAAATGGAGCTTCCGAGCAAGTGCAGAAAGAGGAAGTGGAGCTGCAGACT; TTGCTGACCAACAGCCAGGAGTCCCACAACAGTCACGATGGGAGCGAAGAAGACGTTGAC; ACCTCTGAGCCTATGTACGGCTGCGACATTTGTGGGGCAGCCTACACTATGGAAACTTTG; CTGCAGAATCACCAGCTCCGAGACCACAACATCAGACCTGGAGAAAGTGCCATCGTGAAA; AAGAAAGCTGAGCTCATTAAAGGGAATTACAAGTGCAACGTGTGCTCTCGAACCTTCTTC; TCCGAAAATGGCCTCCGGGAACATATGCAGACCCACCTAGGCCCTGTCAAACACTACATG; TGCCCTATTTGCGGAGAGCGGTTTCCCTCCCTTTTAACTCTTACTGAACACAAAGTCACG; CATAGTAAGAGTCTTGATACTGGAAACTGCCGGATTTGCAAGATGCCTCTCCAGAGTGAA; GAGGAGTTTTTAGAGCATTGCCAAATGCACCCTGACTTGAGGAATTCCCTGACAGGCTTT; CGCTGCGTGGTGTGCATGCAGACAGTGACCTCCACCTTGGAACTCAAAATCCATGGGACG; TTCCACATGCAAAAGACAGGGAATGGGTCTGCAGTTCAGACCACAGGGCGGGGCCAGCAC; GTCCAAAAACTGTATAAGTGCGCATCTTGCCTCAAAGAATTCCGTTCCAAGCAAGATCTG; GTGAAACTTGATATCAATGGCCTGCCATATGGTCTGTGTGCCGGCTGCGTGAATCTCAGT; AAGAGCGCCAGCCCAGGCATTAACGTCCCTCCCGGCACGAATAGACCAGGCTTGGGCCAG; AATGAGAATCTGAGTGCCATTGAGGGGAAAGGCAAGGTGGGGGGACTGAAGACACGCTGC; TCTAGCTGCAACGTTAAGTTTGAGTCTGAAAGTGAACTCCAGAACCACATCCAAACCATC; CACCGAGAGCTCGTGCCAGACAGCAACAGCACACAGTTGAAAACGCCCCAAGTATCACCA; ATGCCCAGAATCAGTCCCTCCCAGTCGGATGAGAAGAAGACCTATCAATGCATCAAGTGT; CAGATGGTTTTCTACAATGAATGGGATATTCAGGTTCATGTTGCAAATCACATGATTGAT; GAAGGACTGAACCATGAATGCAAACTCTGCAGCCAGACCTTTGACTCTCCTGCCAAACTC; CAGTGCCACCTGATAGAGCACAGCTTCGAAGGGATGGGAGGCACCTTCAAGTGTCCAGTC; TGCTTTACAGTATTTGTTCAAGCAAACAAGTTGCAGCAGCATATTTTCTCTGCCCATGGA; CAAGAAGACAAGATCTATGACTGTACACAATGTCCACAGAAGTTTTTCTTCCAAACAGAG; CTGCAGAATCATACAATGACCCAACACAGCAGTTAG; ; Clone variation with respect to NM_015461.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015461 unedited; TGTGCAAATTTTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGCCTCCCTC; CCGCTCCCCGCGCTCTCGCCTTTTAATCATGCCCCTCTGTCTGTGTGTGAGTGCAGGCAG; GCTGACAATGATTTCCTCAGTGATTACGTACAGAGCGAGTCCCTGCGGGTTAGGGGCCCC; CTCTGGAGCCATCCTGATGGCTTTGGGGGCCTTGCTTCCATTTTCCATTATTATGTGGAC; TACCGGAGCGACAGCGCAGTCCAAGACCTTGCAGGATGTCTCGCCGCAAGCAAGCGAAAC; CGAGATCCCTCAAAGACCCCAACTGTAAACTTGAAGACAAGACTGAAGATGGAGAGGCAC; TAGATTGTAAGAAGAGGCCGGAAGACGGGGAGGAGTTGGAAGACGAAGCTGTGCACAGCT; GTGACAGCTGCCTCCAGGTGTTTGAATCGCTGAGCGATATCACAGAACACAAGATTAATC; AATGTCAACTGACAGATGGAGTGGATGTTGAAGATGATCCGACTTGCTCTTGGCCAGCTT; CCTCACCTTCTAGCAAGGATCAGACTTCCCCTAGCCATGGAGAAGGTTGCGATTTTGGAG; AGGAAGAAGGTGGCCCTGGGCTTCCATACCCGTGTCAATTCTGTGACAAGTCGTTTAGCC; GCCTCAGCTACCTAAAGCACCATGAGCAGAGTCACAGTGACAAACTGCCTTTCAAATGCA; CCTACTGCAGTANGCTGTTCAAACACAAGCGCAGCCGAGATCGCCACATAAAACTNCACA; CNNCGGGACAAGAAGTACCACTGCAGTGAATGTGATGCTGCGTTTTCCAGAAGTGATCAC; TTGAAGATCACTTAAGACTACACGTNCACAAGCATATAATGTGCCATTGTCGNCGTGGTT; TCTGTCTCTAGTTCTTACACGACCATGCAGNTCATGAGAAGAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015461 unedited; GGTTAACTATGNACCGCGCCGCATTCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTTGCC; TGAATAGGCCCAAGTCCACTTGTCTTTATAAGACCATTTTAGTATCAGACGACATAATAC; ATGTGCAACACTTTATATACAAGGGGTCTATCCGGTGCGCAAAAGTTCAAACACATGAAC; ATCCAACAGTTTGATAATACAAGTTTTATGGTACAATACAATGTTTCTGAATAATATACA; TTAACAATGAAAGTTTCGTGCAAAGAGTAAAACATGTTCCCTTTTTGTGCCACAAAATCA; ATTCTCCTTGAGAGACTGTACTTGCACTAACTGCTGTGTTGGGTCATTGTATGATTCTGC; AGCTCTGTTTGGAAGAAAAACTTCTGTGGACATTGTGTACAGTCATAGATCTTGTCTTCT; TGTCCATGGGCAGAGAAAATATGCTGCTGCAACTTGTTTGCTTGAACAAATACTGTAAAG; CAGACTGGACACTTGAAGGTGCCTCCCATCCCTTCGAAGCTGTGCTCTATCAGGTGGCAC; TGGAGTTTGGCAGGAGAGTCAAAGGTCTGGCTGCAGAGTTTGCATTCATGGTTCAGTCCT; TCATCAATCATGTGATTTGCAACATGAACCTGAATATCCCATTCATTGTAGAAAACCATC; TGACACTTGATGCATTGATAGGTCTTCTCATCCGACTGGGAGGGACTGATTCTGGGCATT; GGTGATACTTGGGGCGTTTTCAACTGTGTGCTGTTGCTGTCTGGCACGAGCTCTCGGTGG; ATGGTTTGGATGTGGTTCTTGGAGTCACTTTCAGACTCAACTTAACGTTGCAGCTAGAGC; AGCGTGTCTTCAGTCCCCCACCTTGCCTTTCCCCTCATGGCACTCAGATCTCATTTGGNC; CCAGGCTGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_015461
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015461.1, NP_056276.1
• RefSeq Size: 4869 bp
• RefSeq ORF: 3936 bp
• Locus ID: 25925
• UniProt ID: Q96K83
• Cytogenetics: 18q11.2
• Domains: zf-C2H2
• Summary: Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).