NVL (NM_002533) Human Untagged Clone

SKU SC127076

NVL (untagged)-Human nuclear VCP-like (NVL), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NVL
• Synonyms: NVL2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127076 sequence for NM_002533 edited (data generated by NextGen Sequencing); ATGAAGCCCAGACCTGCAGGGTTCGTGGATAATAAACTCAAGCAGCGAGTCATCCAGTAC; CTTACCAGTAACAAATGTGGCAAATATGTGGACATTGGAGTCTTAGCGTCTGATTTACAA; AGAGTGTACAGTATAGACTATGGTCGAAGAAAAAGAAATGCTTTTAGGATTCAGGTAGAA; AAAGTATTTAGCATAATTAGTAGTGAGAAGGAACTTAAGAATTTAACAGAATTAGAAGAT; GAACATTTGGCAAAAAGGGCAAGACAAGGTGAAGAGGATAATGAGTATACTGAAAGCTAT; TCTGATGATGATTCAAGTATGGAAGACTACCCAGATCCACAGTCAGCAAATCACATGAAC; AGTTCCCTGCTGTCTTTATATCGGAAAGGAAATCCTGATTCTGTTTCAAATACTCCTGAG; ATGGAGCAAAGAGAAACCACCTCTTCAACACCACGAATAAGTTCCAAAACAGGCTCCATT; CCCTTGAAGACCCCTGCCAAAGATTCTGAAGGAGGATGGTTTATTGACAAAACCCCAAGT; GTAAAGAAAGACAGTTTTTTCTTGGACCTGTCATGTGAGAAAAGTAATCCTAAGAAGCCA; ATAACTGAGATACAGGATTCAAAAGATTCTTCTCTTTTGGAGAGTGATATGAAACGGAAA; GGCAAGCTAAAGAATAAAGGAAGCAAAAGGAAGAAAGAAGATCTTCAGGAAGTAGATGGA; GAAATTGAAGCTGTCCTACAAAAGAAAGCTAAAGCCAGGGGGTTAGAATTCCAGATCTCC; AACGTGAAGTTTGAAGATGTGGGAGGCAATGATATGACATTAAAAGAGGTCTGCAAGATG; CTCATACACATGCGTCACCCGGAGGTGTACCACCACCTGGGCGTCGTGCCCCCTCGTGGA; GTTCTCCTTCATGGACCACCAGGCTGTGGGAAGACATTACTTGCACATGCAATTGCTGGG; GAACTTGACCTGCCAATTTTGAAAGTGGCTGCTCCAGAGATTGTGTCTGGAGTATCCGGA; GAGTCTGAGCAGAAGCTGAGAGAACTATTTGAGCAAGCTGTGTCAAATGCACCATGTATC; ATTTTCATTGATGAAATTGATGCTATTACCCCCAAAAGAGAAGTGGCTTCAAAAGATATG; GAACGAAGAATTGTAGCCCAACTCCTAACCTGCATGGATGATCTGAATAATGTGGCTGCT; ACAGCCCGGGTCCTAGTTATTGGAGCTACTAATCGACCAGACTCGTTAGACCCTGCTTTG; AGACGTGCGGGAAGGTTCGACCGAGAAATATGCCTAGGTATCCCAGATGAAGCATCCAGG; GAAAGAATACTTCAAACATTGTGCAGAAAACTGAGGCTTCCTCAAGCTTTTGATTTCTGT; CACTTAGCACACCTAACTCCAGGCTTTGTTGGTGCTGATCTCATGGCACTGTGCCGAGAG; GCAGCAATGTGTGCAGTCAATAGAGTCTTAATGAAGCTACAGGAACAGCAGAAGAAAAAT; CCTGAAATGGAAGATTTGCCATCTAAAGGAGTCCAGGAGGAAAGGCTGGGAACTGAGCCC; ACTTCTGAAACACAGGATGAATTACAAAGGCTGCTGGGGTTGCTAAGAGACCAAGATCCC; CTCTCAGAGGAGCAGATGCAAGGACTGTGCATTGAACTGAATGATTTCATTGTTGCTCTA; TCCTCAGTCCAACCCTCTGCCAAAAGGGAAGGCTTTGTCACTGTCCCTAATGTGACATGG; GCAGATATTGGTGCCCTGGAAGACATTAGAGAGGAGCTCACCATGGCAATATTGGCACCA; GTACGCAACCCAGACCAGTTCAAAGCTCTTGGATTGGTGACTCCAGCTGGGGTCCTCCTT; GCTGGTCCTCCTGGCTGTGGGAAGACTCTGCTGGCGAAGGCTGTTGCAAATGAGTCCGGA; CTAAATTTTATATCTGTCAAGGGCCCCGAATTACTAAACATGTATGTTGGTGAGAGTGAA; CGTGCTGTGCGACAAGTTTTTCAACGAGCCAAGAACTCAGCACCCTGTGTGATATTCTTT; GATGAAGTGGATGCTTTATGTCCTCGAAGATCAGACCGAGAGACAGGGGCAAGTGTCCGA; GTGGTGAATCAGCTACTTACAGAGATGGATGGTCTGGAAGCACGCCAGCAGGTTTTTATT; ATGGCAGCCACTAACAGGCCAGATATAATTGACCCTGCAATCCTGCGCCCGGGCCGCCTG; GACAAAACACTGTTTGTGGGTTTACCGCCCCCTGCAGATCGCCTTGCCATCTTAAAAACT; ATCACAAAAAATGGTACCAAACCACCACTGGATGCAGATGTAAATTTGGAAGCAATTGCT; GGTGACCTTCGCTGTGATTGCTATACGGGCGCAGATCTCTCTGCTTTGGTACGAGAAGCT; TCTATCTGTGCCCTGAGACAGGAAATGGCAAGACAGAAGAGTGGAAATGAAAAAGGTGAA; CTCAAGGTTAGTCATAAGCATTTTGAAGAAGCTTTCAAGAAAGTAAGATCATCTATATCA; AAAAAGGATCAAATCATGTATGAACGTTTGCAGGAGTCCCTCAGCCGGTGA; ; Clone variation with respect to NM_002533.3; 456 g=>a;738 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002533 unedited; TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGGCTAGGCCCCAGGA; GGGGCGCATTTCAGGTGCAACTGGCCCGGCGAACGCGGCGGGCAACCCCTACGAGGCCCC; CGGTTGACTAGGAGCTGGCGGTCCGAGCTGTGGCTTGAAAGACCGACGCGATGAAGCCCA; GACCTGCAGGGTTCGTGGATAATAAACTCAAGCAGCGAGTCATCCAGTACCTTACCAGTA; ACAAATGTGGCAAATATGTGGACATTGGAGTCTTAGCGTCTGATTTACAAAGAGTGTACA; GTATAGACTATGGTCGAAGAAAAAGAAATGCTTTTAGGATTCAGGTAGAAAAAGTATTTA; GCATAATTAGTAGTGAGAAGGAACTTAAGAATTTAACAGAATTAGAAGATGAACATTTGG; CAAAAAGGGCAAGACAAGGTGAAGAGGATAATGAGTATACTGAAAGCTATTCTGATGATG; ATTCAAGTATGGAAGACTACCCAGATCCACAGTCAGCAAATCACATGAACAGTTCCCTGC; TGTCTTTATATCGGAAAGGAAATCCTGATTCTGTTTCAAATACTCCTGAGATGGAGCAAA; GAGAAACCACCTCTTCAACACCACGAATAAGTTCCAAAACAGGCTCCATTCCCTTGAAGA; CCCCTGCCAAAGATTCTGAAGGAGGATGGTTTATTGACAAAACCCCCAAGTGTAAGAAAG; ACAGTTTTTTCTTGGACCTGTCATGTGAGAAAGTAATCCTAAGAAGCCATAACTGAGATA; CAGATTCAAAGATNCTTCTCTTTGGAGAGTGATATGAACGGGAAGGCAGCTAAGATAAAG; GAGCANAGGAGAAAGAGATCTTCAGAAGTAATGGAGAAATGAGCTGTCTCAAAGAAGCTA; GCCAGGGGTAGAATCCGATCTCACGTGAGTTGAGAGTGGAGCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002533 unedited; GTACCGCGGCACGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTATTTGAAAAAGTA; ACAGTCATTTTATTTCAGCAGTTTAAAAATGTCCTTTTTCTTAAAGAGGAAGAAGGCATT; TTCACACAAGGCTGGCCAGATGGGAAGAATCTTCAGCTTCAGCTTCAGCTTGGCCTCATT; CATTTGAAAATAAAATGTTTACATGAGGCCGCGCCTGTGTCCAGCTGAAAGTGGGTCCTT; CAGAGCGTGTGGGGGATTCTCTGCCGGCTTGATGGGCTAGCTCCTCTAAGCCGGCTGCTG; GAGACATCACCGGCTGAGGGACTCCTGCAAACGTTCATACATGATTTGATCCTTTTTTGA; TATAGATGATCTTACTTTCTTGAAAGCTTCTTCAAAATGCTTATGACTAACCTTGAGTTC; ACCTTTTTCATTTCCACTCTTCTGTCTTGCCATTTCCTGTCTCAGGGCACAGATAGAAGC; TTCTCGTACCAAAGCAGAGAGATCTGCGCCCGTATAGCAATCACAGCGAAGGTCACCAGC; AATTGCTTCCAAATTTACATCTGCATCCAGTGGTGGTTTGGTACCATTTTTTGTGATAGT; TTTTAAGATGGCAAGGCGATCTGCANGGGGCGGTAAACCCACAAACAGTGTTTTGTCCAG; GCGGCCCGGGCGCAGGATTGCAGGGTCAATTATATCTGGCCTGTTAGTGGCTGCCATAAT; AAAAACCTGCTGGCGGGCTTCCAGACCCTCCATCTCTGTAAGTAGCTGATTCACCACCTC; GAACCCTTGCCCCCTTGTCTCTCGGCCTGAATCTTCGAGGACCTTAAAGCTTCCCCTTCA; TCAAAGAATATTACCACAGGGGCCTGAAGTTCTTGGCTCCGTTGAAAAAACCTTGGGAAA; GGNACGTGCANTCTTAACAACATACCTGGTTTAGAATATCCGGGCCCCCTGCCACAAATA; AAATTAATCCCGGACTCTTTGGAACACACCTGCCCCAGCAAGTTTTTCCCCCGCCCGGAG; GGACACACAGGCGACCCCCNCCTGGGTTCCCTCTCCTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002533
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002533.2, NP_002524.2
• RefSeq Size: 2921 bp
• RefSeq ORF: 2571 bp
• Locus ID: 4931
• UniProt ID: O15381
• Cytogenetics: 1q42.11
• Domains: AAA
• Summary: This gene encodes a member of the AAA (ATPases associated with diverse cellular activities) superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. Two encoded proteins, described as major and minor isoforms, have been localized to distinct regions of the nucleus. The largest encoded protein (major isoform) has been localized to the nucleolus and shown to participate in ribosome biosynthesis (PMID: 15469983, 16782053), while the minor isoform has been localized to the nucleoplasmin. [provided by RefSeq, Aug 2011]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1, also known as NVL.2 or the major isoform).