MED13 (NM_005121) Human Untagged Clone

SKU SC126930

MED13 (untagged)-Human mediator complex subunit 13 (MED13)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MED13
• Synonyms: ARC250; DRIP250; HSPC221; MRD61; THRAP1; TRAP240
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126930 sequence for NM_005121 edited (data generated by NextGen Sequencing); ATGAGTGCCTCCTTCGTGCCGAACGGGGCCAGCCTGGAAGATTGTCACTGTAACCTCTTC; TGCCTGGCTGACTTGACAGGAATTAAGTGGAAAAAATATGTATGGCAAGGCCCAACTTCT; GCCCCTATTCTGTTTCCTGTGACAGAAGAAGACCCCATTTTGAGCAGTTTTAGTCGCTGC; CTTAAGGCAGATGTACTTGGTGTTTGGCGGCGAGATCAAAGACCTGGAAGAAGAGAATTG; TGGATATTTTGGTGGGGTGAAGACCCCAGTTTTGCTGACCTTATTCACCATGACTTATCA; GAAGAAGAAGATGGAGTGTGGGAGAATGGACTTTCCTATGAATGCCGTACTCTGCTTTTC; AAAGCAGTTCACAATCTATTGGAACGGTGTTTAATGAACAGGAATTTTGTACGTATTGGC; AAGTGGTTTGTAAAGCCTTATGAAAAAGATGAAAAACCTATAAATAAAAGTGAACACTTG; TCCTGCTCCTTCACCTTTTTCTTGCATGGAGACAGCAATGTTTGTACCAGTGTGGAAATT; AACCAACATCAACCTGTATACCTTCTCAGTGAAGAGCATATCACCCTTGCTCAACAGTCT; AATAGCCCATTTCAAGTTATCTTATGCCCATTTGGACTAAATGGCACTCTCACAGGACAG; GCATTCAAGATGTCTGATTCAGCTACAAAAAAATTAATTGGTGAATGGAAACAGTTCTAT; CCTATCTCATGTTGCTTGAAGGAGATGTCTGAAGAAAAACAGGAAGATATGGATTGGGAA; GATGATTCTTTAGCTGCAGTAGAAGTTCTTGTTGCTGGTGTCCGAATGATCTACCCAGCA; TGCTTTGTTCTAGTCCCTCAGTCAGACATTCCTACTCCTAGCCCTGTGGGATCCACTCAC; TGTTCATCTTCTTGCTTGGGTGTCCACCAAGTGCCTGCTTCCACAAGAGATCCTGCTATG; TCTTCGGTTACGCTTACACCACCTACGTCTCCTGAGGAAGTCCAAACAGTTGATCCTCAG; TCTGTCCAGAAGTGGGTCAAATTTTCTTCAGTATCTGATGGCTTCAACTCCGATAGTACT; AGCCACCATGGTGGGAAAATACCCAGAAAATTAGCAAATCATGTGGTGGATAGAGTTTGG; CAAGAATGCAATATGAACAGAGCACAGAACAAGAGGAAGTATTCTGCTTCATCAGGTGGT; CTATGCGAAGAAGCGACAGCTGCTAAAGTGGCATCCTGGGATTTTGTTGAAGCCACACAA; AGAACAAATTGCAGTTGTTTGAGGCACAAAAATCTCAAGTCAAGAAATGCTGGACAACAA; GGACAGGCACCATCTTTAGGTCAGCAACAACAAATACTTCCTAAGCACAAGACCAATGAG; AAGCAAGAAAAGAGTGAAAAGCCACAGAAACGCCCCTTGACTCCTTTTCACCATCGTGTG; TCTGTTAGTGATGATGTTGGCATGGACGCAGATTCAGCCAGCCAAAGACTTGTGATCTCT; GCTCCAGACAGTCAAGTGAGATTTTCAAATATCCGAACTAATGATGTAGCAAAGACTCCT; CAGATGCATGGCACCGAAATGGCAAATTCACCTCAACCACCCCCACTTAGTCCTCACCCT; TGTGATGTGGTTGATGAAGGAGTGACTAAAACACCTTCAACTCCTCAGAGTCAACATTTT; TATCAAATGCCAACACCAGATCCCTTGGTTCCTTCTAAACCAATGGAAGATAGGATAGAC; AGTTTGTCCCAGTCTTTCCCACCTCAATATCAGGAAGCTGTAGAACCTACAGTATATGTT; GGTACAGCAGTAAACTTGGAAGAAGATGAAGCCAATATAGCCTGGAAGTATTACAAGTTC; CCAAAGAAAAAAGATGTAGAGTTTTTACCACCTCAACTTCCAAGTGATAAATTCAAGGAT; GATCCAGTTGGACCTTTTGGACAGGAAAGTGTAACATCAGTTACAGAGTTAATGGTGCAA; TGTAAGAAACCTTTAAAAGTTTCTGATGAATTAGTGCAGCAATATCAAATTAAAAACCAG; TGTCTTTCAGCAATAGCATCTGATGCAGAACAAGAACCTAAAATTGATCCATATGCATTT; GTTGAAGGAGATGAGGAATTCCTTTTTCCTGATAAAAAAGATAGACAAAATAGTGAGAGA; GAAGCTGAAAAAAAACACAAGGTAGAAGATGGGACATCTAGTGTAACAGTGTTATCACAT; GAAGAAGATGCTATGTCATTATTTAGTCCCTCTATCAAGCAAGATGCTCCACGCCCTACT; AGTCATGCCCGTCCTCCATCAACAAGTTTGATTTATGACTCAGACCTGGCTGTCTCTTAT; ACTGACCTTGATAATCTCTTCAATTCTGATGAAGATGAACTAACACCTGGATCTAAAAAA; TCAGCAAATGGATCAGATGATAAAGCCAGCTGCAAGGAATCAAAGACAGGAAATCTGGAC; CCGTTATCTTGCATAAGCACTGCAGATCTTCATAAAATGTATCCTACACCACCATCATTG; GAACAACATATTATGGGATTTTCCCCAATGAATATGAATAATAAAGAATATGGTAGTATG; GATACAACACCTGGAGGAACTGTTCTAGAAGGAAATAGTTCTAGTATAGGAGCGCAGTTC; AAAATTGAGGTTGATGAGGGATTCTGTAGCCCCAAACCTTCTGAAATTAAAGATTTTTCT; TATGTCTATAAGCCTGAAAATTGTCAAATTCTAGTGGGATGTTCCATGTTTGCACCTCTA; AAAACTCTACCAAGCCAATATCTGCCCCCTATCAAATTGCCAGAAGAGTGTATTTACCGT; CAGAGTTGGACTGTTGGAAAATTGGAATTGCTTTCTTCAGGGCCTTCAATGCCATTCATC; AAAGAGGGTGATGGAAGTAATATGGATCAAGAATATGGCACTGCTTATACACCTCAAACT; CATACTTCTTTTGGGATGCCTCCTAGCAGTGCACCTCCTAGTAACAGCGGAGCAGGAATT; CTTCCTTCTCCATCCACCCCTCGGTTTCCAACTCCAAGGACTCCAAGGACTCCTCGGACT; CCTCGTGGAGCTGGTGGACCTGCTAGTGCTCAAGGTTCAGTCAAATATGAAAATTCAGAC; TTGTATTCACCAGCTTCTACCCCATCTACATGCAGACCCCTTAATTCTGTTGAACCTGCA; ACTGTCCCTTCCATCCCTGAAGCACACAGTCTTTATGTAAACCTCATCCTTTCAGAATCA; GTTATGAATTTGTTTAAAGACTGTAACTTTGATAGTTGTTGCATCTGTGTTTGCAACATG; AACATCAAGGGTGCCGATGTTGGAGTTTACATTCCAGATCCAACGCAGGAAGCACAATAT; AGGTGTACCTGTGGCTTCAGTGCTGTCATGAACAGAAAATTTGGAAACAATTCAGGATTA; TTTCTTGAAGATGAACTAGATATCATAGGACGCAATACAGACTGTGGCAAAGAAGCAGAA; AAACGTTTTGAAGCTCTCAGGGCTACCTCTGCTGAACATGTTAATGGAGGACTAAAGGAA; TCTGAAAAATTATCTGATGATTTGATATTATTGCTACAAGATCAGTGCACTAATTTATTT; TCACCCTTTGGAGCAGCAGACCAAGATCCTTTTCCTAAAAGTGGTGTAATTAGCAATTGG; GTACGTGTTGAAGAGCGTGACTGTTGCAATGACTGCTACCTTGCATTAGAACATGGGCGT; CAGTTCATGGATAACATGTCAGGAGGAAAAGTTGATGAAGCACTTGTGAAAAGTTCATGC; TTACACCCCTGGTCCAAAAGAAACGATGTGAGTATGCAGTGCTCACAGGATATACTTCGA; ATGCTCCTCTCTCTTCAGCCAGTTCTTCAGGATGCCATTCAGAAAAAAAGAACAGTAAGA; CCTTGGGGTGTTCAGGGTCCTCTCACTTGGCAACAATTTCATAAAATGGCTGGCCGAGGC; TCTTATGGAACTGATGAATCCCCAGAACCACTGCCAATCCCCACATTTTTGTTGGGTTAT; GATTATGATTATCTGGTGCTTTCTCCATTTGCTCTTCCTTATTGGGAGAGACTTATGCTG; GAACCCTATGGATCTCAAAGAGATATAGCCTATGTTGTACTGTGTCCAGAAAATGAAGCC; TTGTTAAATGGAGCAAAAAGCTTTTTTAGAGATCTTACTGCAATATATGAGTCCTGTCGA; TTAGGTCAACATAGACCTGTTTCTCGACTGTTAACAGATGGGATCATGAGAGTTGGATCT; ACTGCATCAAAGAAACTATCAGAAAAGTTGGTAGCAGAATGGTTTTCTCAGGCAGCTGAT; GGTAACAATGAAGCATTTTCTAAACTCAAGCTTTATGCACAAGTCTGCAGATATGACCTA; GGTCCTTATCTTGCTTCCCTGCCATTGGACAGCTCTCTACTTTCCCAGCCAAATTTAGTT; GCCCCTACAAGTCAGTCTTTGATTACTCCACCTCAGATGACAAATACTGGAAATGCTAAT; ACTCCATCTGCCACCTTAGCATCTGCAGCGAGCAGCACTATGACAGTGACTTCAGGTGTT; GCCATATCTACTTCAGTTGCCACAGCTAATTCAACTTTGACCACAGCTTCAACTTCATCT; TCATCATCCTCCAACTTGAATAGTGGAGTATCATCAAATAAACTACCTTCGTTTCCACCC; TTTGGCAGTATGAACAGTAATGCTGCAGGATCCATGTCTACACAAGCAAATACAGTTCAG; AGTGGTCAGCTAGGAGGGCAACAGACATCAGCTCTACAGACAGCTGGGATTTCTGGAGAA; TCATCTTCACTTCCCACTCAGCCGCATCCTGATGTGTCTGAAAGCACGATGGATCGGGAT; AAAGTGGGAATCCCCACAGATGGTGATTCACATGCAGTCACGTATCCACCTGCAATTGTT; GTTTATATAATTGATCCTTTTACATACGAAAATACAGACGAGAGCACTAACTCTTCTAGT; GTGTGGACATTGGGGCTACTTCGATGCTTTCTAGAAATGGTCCAGACTCTTCCTCCTCAT; ATCAAGAGTACTGTTTCTGTACAGATTATTCCTTGTCAGTACCTGTTGCAACCTGTGAAG; CATGAAGATAGAGAAATCTATCCCCAGCATTTAAAATCCCTGGCTTTTTCGGCCTTTACC; CAGTGTCGGAGGCCACTTCCAACATCAACCAATGTGAAAACATTGACTGGCTTTGGTCCA; GGTTTAGCCATGGAAACTGCCCTTAGAAGTCCTGATAGACCAGAGTGTATTCGACTTTAT; GCACCTCCTTTTATTCTGGCTCCAGTGAAGGACAAACAGACAGAGCTAGGAGAAACATTT; GGAGAAGCTGGACAGAAATATAATGTTCTTTTTGTGGGATACTGTTTATCACATGATCAA; AGGTGGATTCTTGCATCTTGCACAGATCTATATGGAGAACTTTTAGAAACTTGTATCATT; AACATCGATGTTCCAAATAGGGCTCGTCGGAAAAAAAGTTCTGCTAGAAAATTTGGTCTA; CAGAAACTTTGGGAGTGGTGCTTAGGACTTGTACAAATGAGTTCATTGCCATGGAGAGTT; GTAATTGGTCGTCTAGGAAGGATTGGTCATGGAGAATTGAAAGATTGGAGCTGTTTGCTG; AGTCGTCGAAACTTGCAGTCTCTAAGTAAAAGGCTCAAAGACATGTGTAGAATGTGTGGT; ATATCTGCTGCAGACTCCCCTAGCATTCTCAGTGCTTGCTTGGTGGCAATGGAGCCGCAA; GGCTCTTTTGTTATTATGCCAGATTCTGTGTCAACTGGTTCTGTATTTGGAAGAAGCACG; ACTCTAAATATGCAGACATCTCAGCTAAATACCCCACAGGATACATCATGTACTCATATA; CTTGTGTTTCCTACTTCTGCTTCTGTGCAAGTAGCTTCAGCTACTTATACCACTGAAAAT; TTGGATTTAGCTTTCAATCCCAACAATGATGGAGCAGATGGAATGGGTATCTTTGATTTG; TTAGACACAGGAGATGATCTTGACCCTGATATCATTAATATCCTTCCTGCTTCTCCAACT; GGTTCTCCTGTACATTCTCCAGGATCTCATTACCCCCATGGAGGTGATGCGGGCAAGGGT; CAGAGTACTGATCGGCTACTATCAACAGAACCTCATGAGGAAGTACCTAATATTCTTCAG; CAACCATTGGCCCTTGGTTACTTTGTATCAACTGCCAAAGCAGGTCCATTACCTGACTGG; TTCTGGTCAGCATGTCCTCAAGCACAATATCAGTGTCCCCTTTTTCTTAAGGCCTCTTTG; CACCTCCACGTGCCTTCAGTGCAATCTGACGAGCTGCTTCACAGTAAACACTCCCACCCA; CTTGACTCAAATCAGACTTCAGATGTCCTCAGGTTTGTTTTGGAACAGTACAATGCACTC; TCCTGGCTAACCTGTGACCCTGCAACCCAGGACAGACGCTCATGTCTCCCAATTCATTTT; GTGGTGCTGAATCAGTTATATAACTTTATTATGAATATGCTGTGA; ; Clone variation with respect to NM_005121.2; 2168 g=>a;4320 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005121 unedited; GTTTAGATTTTGTAATACGACTTATTATAGGGCGGCCGCGAAATTCGCACGAGGCCCGGT; GTCTGTGTTTCTCTCTCTGGTCGGAGGCGGCGGTAATGGCGGATGGTGGGTTGTGGCGCC; GGCGGCGGCTGCTGTGAGGGACGATGAGTGCCTCCTTCGTGCCGAACGGGGCCAGCCTGG; AAGATTGTCACTGTAACCTCTTCTGCCTGGCTGACTTGACAGGAATTAAGTGGAAAAAAT; ATGTATGGCAAGGCCCAACTTCTGCCCCTATTCTGTTTCCTGTGACAGAAGAAGACCCCA; TTTTGAGCAGTTTTAGTCGCTGCCTTAAGGCAGATGTACTTGGTGTTTGGCGGCGAGATC; AAAGACCTGGAAGAAGAGAATTGTGGATATTTTGGTGGGGTGAAGACCCCAGTTTTGCTG; ACCTTATTCACCATGACTTATCAGAAGAAGAAGATGGAGTGTGGGAGAATGGACTTTCCT; ATGAATGCCGTACTCTGCTTTTCAAAGCAGTTCACAATCTATTGGAACGGTGTTTAATGA; ACAGGAATTTTGTACGTATTGGCAAGTGGTTTGTAAAGCCTTATGAAAAAGATGAAAAAC; CTATAAATAAAAGTGAACACTTGTCCTGCTCCTTCACCTTTTTCTTGCATGGAGACCAGC; ATGTTTGTACCAGTGTGGAAATTAACCAACATCAACCTGTATACCTTCTCAGTGAAGAGC; ATATCACCCTTGCTCAACAGTCTAATAGCCCATTTCAAGTTATCTTATGCCCATTTGGAC; TAAATGGCACTCTCACCNAGACAGCATTCAAGATGTCTGATTCAGCTACAAANAAATTAA; TGGTGAATGGAAACAGTTCTATCCTATCTCATGNNTGCNGAANGNANNATGTCTGAGAAA; AACAGNAGATAATGATTGGGAGATGAATCTTAGCTGCGTAGAAGTN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005121 unedited; TATTTATGGACCGCGGGCCGCAATCTANGGATCGGTTTTTTTTTTTTTTTTTTAAAGCAG; AAAGGCAGTATAAACCTTCTAGGAAAATTTTTATAAAAGAGGTTAAGAAATATAAGACAA; TTTATACATTTAAAAACTTACAATAAATAAGAGAAGAGATGCAGGCATTTTTGAATACTA; GATACTATAATCCATACAAGAACATCTTCATGTTCTGAAGCCATATGTTGAAATTCATTG; TTCCTCATGTTTCCTCTCCATGCTTTTAATATTCATTTAACATGACTGGGTACTATGGCT; CATTACTTTTCACAAATACTGTGACTGGAAAAAAAAAAGGTTAATTTTGCTACGAAAATG; TTATAATACGTTTTGTATGATCAGTCATCATCCTAAAGAGTTCAATAGAGTCAATGAAAA; ATAGCAGGGTTATAGCCCCTCCCCCCAAGTCAAAACAATATTTGTTGAAGTAATAAGAAT; TAGACCCCATCACAAATGACCTTCACTGAGAAGATAATTGTAACTTAATCCTGGAGGGAA; ACATCACATTTTTAGCTTGATCTTTTCTTGGCCAGCGCCATCAAATGAAGACCGCAGGGT; ATTCCTGACAAAGTTATTTAACCTGATCGCCCTCCCCGAAATGAATCGCGAGACATGACC; GACTGAACCGGGATGTAGGATTCCCCGTCACCCCCGAGAGGGCATTGAGCTGACCGCAAA; CAACCCTGGAGACCCCGGAACACTGACTAGACCTTAGAGGTGGGGAGGGGCTATTGAGCA; ACGGAGCGGGTAGATTGCCCTGGAGGCGCCGGCGGGCGAAGCCAGGCAATAAGAAACGGG; GGCCCTGCAATTGTCAGTGGGGACTGTTTGTACCCACCGATTTAGTCATGGGGGCCGCCT; CGGCCCGACACACCCGCCCAACTNGGCTGATCGGCGTCGCCGAGAACAACGGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_005121
• Insert Size: 7180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005121.1, NP_005112.1
• RefSeq Size: 7389 bp
• RefSeq ORF: 6525 bp
• Locus ID: 9969
• UniProt ID: Q9UHV7
• Cytogenetics: 17q23.2
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator. [provided by RefSeq, Jul 2008]