PJA2 (NM_014819) Human Untagged Clone

SKU SC126706

PJA2 (untagged)-Human praja ring finger 2 (PJA2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PJA2
• Synonyms: Neurodap1; RNF131
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126706 sequence for NM_014819 edited (data generated by NextGen Sequencing); ATGTCACAGTACACTGAAAAGGAGCCAGCAGCAATGGACCAAGAATCTGGTAAGGCTGTC; TGGCCCAAACCAGCAGGAGGGTATCAGACAATTACAGGCAGGAGATATGGAAGAAGACAT; GCTTATGTCAGTTTTAAACCATGTATGACCAGACATGAAAGAAGCTTAGGTCGGGCTGGT; GATGACTATGAAGTGTTGGAACTAGATGATGTTCCAAAGGAAAATTCCTCAGGTTCCAGT; CCTTTGGATCAAGTTGATTCTTCTTTACCCAGTGAACCTATATTTGAAAAAAGTGAAACA; GAAATTCCCACTTGTGGTTCAGCATTGAATCAAACCACTGAGAGCAGTCAATCCTTTGTT; GCAGTACATCACAGTGAGGAAGGCAGGGATACCTTAGGAAGCAGTACAAATCTTCATAAT; CACTCTGAGGGAGAGTATATTCCAGGAGCTTGTAGTGCTTCAAGTGTCCAAAATGGAATT; GCATTGGTTCATACAGACTCTTATGATCCAGATGGCAAACATGGAGAAGATAATGACCAT; CTTCAACTTTCTGCAGAAGTCGTGGAAGGTAGTAGATACCAGGAATCATTAGGCAATACA; GTATTTGAGTTGGAAAACAGAGAGGCAGAGGCATACACTGGTCTTTCACCACCAGTTCCC; TCATTTAACTGTGAAGTAAGAGATGAGTTTGAAGAGTTAGATTCTGTACCATTAGTGAAA; AGTTCTGCTGGTGATACTGAGTTTGTCCATCAGAATAGCCAGGAAATTCAGAGGTCTTCT; CAAGATGAAATGGTTAGTACGAAACAACAAAATAATACTAGCCAGGAAAGACAGACAGAA; CATTCACCTGAAGATGCAGCCTGTGGTCCAGGGCATATTTGTAGTGAACAAAATACCAAT; GATAGGGAAAAGAACCATGGAAGTTCTCCTGAACAGGTAGTGAGGCCAAAAGTTAGAAAA; CTGATAAGTTCAAGCCAGGTGGACCAAGAAACAGGTTTTAATAGGCATGAGGCGAAACAA; AGAAGTGTTCAAAGATGGAGAGAGGCTTTGGAAGTTGAGGAAAGTGGCTCAGATGACCTC; TTAATAAAATGTGAAGAATATGATGGAGAGCATGACTGTATGTTCTTGGATCCACCATAC; TCAAGAGTTATTACACAAAGGGAAACAGAAAATAACCAAATGACATCAGAAAGTGGAGCC; ACAGCAGGAAGGCAAGAAGTGGATAACACCTTTTGGAATGGCTGTGGAGATTATTACCAA; CTCTATGACAAAGATGAAGATAGTTCTGAATGCAGTGATGGGGAATGGTCTGCTTCTTTG; CCTCATCGATTTTCTGGTACAGAAAAAGATCAATCCTCAAGTGATGAAAGCTGGGAGACT; CTGCCAGGAAAAGATGAGAATGAACCTGAGCTACAAAGTGATAGCAGTGGCCCTGAAGAA; GAAAACCAAGAATTATCTCTTCAGGAAGGGGAACAGACATCCTTGGAAGAGGGAGAAATT; CCTTGGTTACAGTACAATGAAGTCAATGAAAGCAGCAGTGATGAGGGAAATGAACCTGCC; AATGAATTTGCACAGCCAGCTTTCATGTTGGATGGTAACAATAACCTGGAGGATGACTCC; AGTGTGAGTGAAGACTTAGATGTGGATTGGAGCCTATTTGATGGCTTTGCAGATGGACTA; GGAGTTGCTGAAGCTATTTCATATGTGGATCCTCAGTTCCTTACCTACATGGCACTAGAA; GAACGCTTAGCCCAGGCTATGGAGACTGCTCTGGCCCATTTAGAGTCTCTTGCAGTGGAT; GTTGAGGTGGCCAATCCACCAGCTAGTAAGGAAAGCATTGATGGTCTTCCAGAGACCCTT; GTTCTTGAAGATCACACTGCTATTGGTCAGGAACAATGCTGTCCAATCTGTTGCAGTGAG; TATATTAAGGATGATATAGCAACAGAGTTGCCCTGTCACCATTTCTTTCACAAACCTTGT; GTCTCAATTTGGCTACAAAAGTCGGGAACATGCCCTGTGTGCCGCCGTCATTTCCCACCT; GCGGTTATTGAAGCATCTGCAGCTCCTTCCTCTGAGCCTGATCCTGATGCCCCACCTTCA; AATGACAGTATTGCAGAAGCACCCTAA; ; Clone variation with respect to NM_014819.4; 1218 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014819 unedited; GCCGCGAATTCGGCACCAGGCTTCGCGTTCGGCGGGGGAGGAGTTGGAGGCGGAGAAGAA; GGCGGTGGTGGCGGGTGGAGGTTCGAGCGCTGTTCTCGCTCCGGAGCCGCTGCACATTTC; GGAATCTTCTGCGGCTTGTCCATAGTGTGAATAAAAACTAAATCACATCTATAATTCTAC; TGAACTGGTCACACAGACGCTGCCATATATGTCACAGTACACTGAAAAGGAGCCAGCAGC; AATGGACCAAGAATCTGGTAAGGCTGTCTGGCCCAAACCAGCAGGAGGGTATCAGACAAT; TACAGGCAGGAGATATGGAAGAAGACATGCTTATGTCAGTTTTAAACCATGTATGACCAG; ACATGAAAGAAGCTTAGGTCGGGCTGGTGATGACTATGAAGTGTTGGAACTAGATGATGT; TCCAAAGGAAAATTCCTCAGGTTCCAGTCCTTTGGATCAAGTTGATTCTTCTTTACCCAG; TGAACCTATATTTGAAAAAAGTGAAACAGAAATTCCCACTTGTGGTTCAGCATTGAATCA; AACCACTGAGAGCAGTCAATCCTTTGTTGCAGTACATCACAGTGAGGAAGGCAGGGATAC; CTTAGGAAGCAGTACAAATCTTCATAATCACTCTGAGGGAGAGTATATTCCAGGAGCTTG; TAGTGCTTCAAGTGTCCAAATGGAATTGCATTGGTTCATACAGACTCTTATGATCCAGAT; GGGCAACATGGAGAAAGATAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014819 unedited; GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATT; TAAGCCAACTCTTATTCAACTTTTCTTCTTCACAGCAGCTGTTTATAGATAGTAGGGAGC; CAAGAATGAAGGACAGTAACAGATGGAAAGCAAAAAGTACAACAGCTATCTTAAGTTCAG; CTCTCAACATTGCTGGTTGAGTTTGGAACCAAAACCTCTTAACAACTGGCAGATAATAGC; TAAATCTTAACAGACAAAGAAGAAATATTTTCTTTGGGACAGCTGCTATCTAAAAAAAAA; CCAAGGTCCCTTAATATAGTCTAAATATAATGTGTGGCTTATTATAGAGAAATCTTTAGC; AACGTAAGTTTAACCAGTAAGTGTCACAACTGATCAACAGTACTTAAAAGGAAACAAACA; AAAATCACACTAGCCACAAATTTCCACCATATACACATGAAATTAATTTTAATCTGTTTT; GACTCCTTGACACTAACTGATCATTAATGAAATATGATATGGAAAGATCACAGAGTAGAA; AACAAGCAAAGATTAGTTTATACAACAGTGACTATATACATCAGAGGGAAAACATGCTAG; CTAATGCAACATTAAGGCCTGAATGTAAGCATTCCCCAAGCCACAGAAGCCCCAAAGAAC; TCCTAAATTACAAATTCATCACATTACATGCATGCCATGTTCACTTTTGTTTTACCCATA; AAGGGATACACCGATTTTTGCTGTAAATACCCAGACCCTTNTACAATATATGCAAAAATT; AGCATCGCAAGTGTTTTGTTCCTTATTTTTAACCCTCTATTGGCCAACAGCGAAAATTTA; TTCTTTTCAAAATGAAAAAGCGCACGGCTCTAACCTCTAAAAACACCCCATCAAAAGACC; TTTGTTTTGGCAGACTATAAACTTCTTTTTGTTCCCAACCATTTTCAGGCCACCCAACGC; ACACCCCCCTCGGGTTAACTTACCCTGGCACAGGTCCCACGCCCCTCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014819
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014819.2, NP_055634.2
• RefSeq Size: 4789 bp
• RefSeq ORF: 2127 bp
• Locus ID: 9867
• UniProt ID: O43164
• Cytogenetics: 5q21.3
• Domains: RING
• Protein Families: Druggable Genome
• Summary: Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes. Through the ubiquitination of MFHAS1, positively regulates the TLR2 signaling pathway that leads to the activation of the downstream p38 and JNK MAP kinases and promotes the polarization of macrophages toward the pro-inflammatory M1 phenotype (PubMed:28471450).[UniProtKB/Swiss-Prot Function]