CD63 (NM_001780) Human Untagged Clone

CAT#: SC126650

CD63 (untagged)-Human CD63 molecule (CD63), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD63
• Synonyms: LAMP-3; ME491; MLA1; OMA81H; TSPAN30
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126650 sequence for NM_001780 edited (data generated by NextGen Sequencing)
  ATGGCGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTGCTGGCC
  TTTTGCGCCTGTGCAGTGGGACTGATTGCCGTGGGTGTCGGGGCACAGCTTGTCCTGAGT
  CAGACCATAATCCAGGGGGCTACCCCTGGCTCTCTGTTGCCAGTGGTCATCATCGCAGTG
  GGTGTCTTCCTCTTCCTGGTGGCTTTTGTGGGCTGCTGCGGGGCCTGCAAGGAGAACTAT
  TGTCTTATGATCACGTTTGCCATCTTTCTGTCTCTTATCATGTTGGTGGAGGTGGCCGCA
  GCCATTGCTGGCTATGTGTTTAGAGATAAGGTGATGTCAGAGTTTAATAACAACTTCCGG
  CAGCAGATGGAGAATTACCCGAAAAACAACCACACTGCTTCGATCCTGGACAGGATGCAG
  GCAGATTTTAAGTGCTGTGGGGCTGCTAACTACACAGATTGGGAGAAAATCCCTTCCATG
  TCGAAGAACCGAGTCCCCGACTCCTGCTGCATTAATGTTACTGTGGGCTGTGGGATTAAT
  TTCAACGAGAAGGCGATCCATAAGGAGGGCTGTGTGGAGAAGATTGGGGGCTGGCTGAGG
  AAAAATGTGCTGGTGGTAGCTGCAGCAGCCCTTGGAATTGCTTTTGTCGAGGTTTTGGGA
  ATTGTCTTTGCCTGCTGCCTCGTGAAGAGTATCAGAAGTGGCTACGAGGTGATGTAG
  
  Clone variation with respect to NM_001780.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001780 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGCCGCGGCGGGAGA
  GGAACGCGCAGCCAGCCTTGGGAAGCCCAGGCCCGGCAGCCATGGCGGTGGAAGGAGGAA
  TGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTGCTGGCCTTTTGCGCCTGTGCAGTGG
  GACTGATTGCCGTGGGTGTCGGGGCACAGCTTGTCCTGAGTCAGACCATAATCCAGGGGG
  CTACCCCTGGCTCTCTGTTGCCAGTGGTCATCATCGCAGTGGGTGTCTTCCTCTTCCTGG
  TGGCTTTTGTGGGCTGCTGCGGGGCCTGCAAGGAGAACTATTGTCTTATGATCACGTTTG
  CCATCTTTCTGTCTCTTATCATGTTGGTGGAGGTGGCCGCAGCCATTGCTGGCTATGTGT
  TTAGAGATAAGGTGATGTCAGAGTTTAATAACAACTTCCGGCAGCAGATGGAGAATTACC
  CGAAAAACAACCACACTGCTTCGATCCTGGACAGGATGCAGGCAGATTTTAAGTGCTGTG
  GGGCTGCTAACTACACAGATTGGGAGAAAATCCCTTCCATGTCGAAGAACCGAGTCCCCG
  ACTCCTGCTGCATTAATGTTACTGTGGGCTGTGGGATTAATTTCAACGAGAAGGCGATCC
  ATAAGANGGCTGTGTGGAAATNAATTGNGGGCNNTGCTGAGAAAAATGTGCCTGGTGGTA
  GCTGCANCAGCCCTTGGAATGCTTTGNNCNAAGGNTTTGGGAAATTGTCTTTGCCTGCTG
  CCTCGTGAAGATTTCANAAGTGCCTACAGGTGNTTTNGGGGGGGNTGTGTCTCTCCAACC
  CTCTATTGGGGGGGAAAGGGAAAAGAATCCCTCCAAGTTTTCAATAAACGGTATATTNTT
  TTAAACCGAAAAAAAAAAAAAACTCGACTTAAATGGGGGGCCGGGCTTATACTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001780 unedited
  GGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCGGTCTGAAAAAAA
  ATCCGTTTAATTGAAAAACCTGGAGGATACTATTCCACTCCCCCAGATGAGGAGGCTGAG
  GAGACCAGACCCCTACATCACCTCGTAGCCACTTCTGATACTCTTCACGAGGCAGCAGGC
  AAAGACAATTCCCAAAACCTCGACAAAAGCAATTCCAAGGGCTGCTGCAGCTACCACCAG
  CACATTTTTCCTCAGCCAGCCCCCAATCTTCTCCACACAGCCCTCCTTATGGATCGCCTT
  CTCGTTGAAATTAATCCCACAGCCCACAGTAACATTAATGCAGCAGGAGTCGGGGACTCG
  GTTCTTCGACATGGAAGGGATTTTCTCCCAATCTGTGTAGCTAGCAGCCCCACAGCACTC
  AAAATCTGCCTGGCTCCTGTCCAGGATCGAAGCAGCGTGGACGGCCACGGGGAAATCCCT
  CATCTGCTGCGGGCAGCTGCCTGCAACTCGTATCCCCCCCCTCCCACCACACCCCCCTCC
  TGGGCACGCTACCCCCCCCCACCTCACGCACCCCCCCCCCACATCAACCTCCTCCCCCCC
  TTTTACCCCAATCCCCCTACCCCAACTCTCCCCCCCCTTCCCCACCTCTCCCGACTCCTC
  GCCCCCCCCCCCCCCCCCCCCCACCCCCCCCTCCTCTCCCTTCCCCTTCTCCTCCGTATT
  TATCTCTCTCATCCACCCCGCTTTTTTCCCATTCGCCCCTCTCGCAAACTCCGTTCCTCC
  CTCCCCCCTATTCCCCCCCCGCCATTCCCCTCCCCCCCCCGTTTCCCTCCCTACGCCTCC
  CATCCTTTTTCCCAGTCCCGCTCGTCCCCCTCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001780
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001780.3, NP_001771.1
• RefSeq Size: 916 bp
• RefSeq ORF: 717 bp
• Locus ID: 967
• UniProt ID: P08962
• Cytogenetics: 12q13.2
• Domains: transmembrane4
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome
• Gene Summary: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (1) encodes the longest isoform (A). Variants 1, 3, 4, 5 and 10 encode the same isoform A.