BAAT1 (BRAT1) (NM_152743) Human Untagged Clone

SKU SC126644

BRAT1 (untagged)-Human BRCA1-associated ATM activator 1 (BRAT1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: BAAT1
• Synonyms: BAAT1; C7orf27; NEDCAS; RMFSL
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126644 sequence for NM_152743 edited (data generated by NextGen Sequencing); ATGGACCCAGAATGCGCCCAGCTGCTCCCGGCTCTCTGTGCTGTTCTGGTAGATCCCAGG; CAGCCGGTGGCAGATGACACCTGTTTGGAGAAGCTCCTGGACTGGTTTAAAACGGTCACT; GAAGGAGAGTCCAGTGTCGTGCTGCTGCAGGAGCACCCCTGCCTGGTGGAGCTGCTGTCC; CATGTGCTGAAAGTCCAGGACCTGAGTTCTGGGGTCCTCTCCTTCTCACTGCGCCTGGCA; GGAACCTTCGCAGCCCAGGAAAACTGCTTCTAGTATCTTCAGCAGGGGGAGTTACTACCA; GGGCTCTTTGGGGAGCCAGGACCCCTCGGCCGAGCAACCTGGGCCGTCCCCACCGTGCGC; AGCGGCTGGATCCAGGGCCTGCGCTCCCTGGCACAGCACCCCAGCGCCCTGCGCTTCCTG; GCCGACCATGGTGCGGTCGACACCATCTTCTCCCTGCAGGGAGACTCCAGCCTGTTTGTG; GCCTCGGCGGCCAGTCAGCTCCTGGTGCACGTCCTGGCTTTGTCCATGCGAGGTGGAGCC; GAGGGGCAGCCCTGCCTGCCGGGGGGTGACTGGCCCGCGTGTGCCCAGAAGATCATGGAT; CACGTTGAAGAGTCCTTGTGCTCCGCGGCCACCCCCAAGGTCACTCAGGCCCTGAACGTC; CTGACCACGACCTTCGGGCGCTGCCAGAGCCCCTGGACAGAAGCCCTGTGGGTGCGGCTG; AGTCCCCGCGTGGCCTGTCTGCTGGAGAGAGACCCCATCCCCGCCGCACACTCGTTCGTG; GACCTGCTTCTCTGTGTGGCTCGTTCTCCCGTGTTCAGTTCTTCCGACGGCAGCCTGTGG; GAGACAGTGGCGCGGGCTCTGAGCTGCCTGGGTCCCACCCACATGGGACCCCTGGCTTTG; GGGATCCTGAAGCTCGAGCACTGTCCACAGGCACTGAGGACCCAGGCCTTCCAGGTCCTT; CTCCAGCCCCTGGCCTGTGTCCTGAAGGCCACGGTTCAGGCCCCCGGACCCCCAGGCTTG; CTGGACGGGACGGCAGACGATGCCACGACGGTGGACACACTCCTGGCCTCCAAGTCGTCC; TGCGCCGGCCTCCTGTGCCGCACCCTGGCTCACCTGGAGGAGCTGCAGCCGCTGCCCCAG; CGCCCTTCACCGTGGCCCCAGGCGTCTCTACTGGGGGCTACAGTGACTGTCCTGCGGCTC; TGTGACGGCTCGGCTGCCCCTGCCTCCAGTGTGGGGGGCCACCTCTGTGGGACCCTGGCG; GGCTGCGTCCGGGTCCAGCGAGCAGCCCTCGACTTCCTGGGGACGCTGTCACAGGGGACA; GGCCCCCAGGAGCTGGTGACGCAGGCGCTTGCTGTCCTCCTGGAGTGCCTCGAGAGCCCC; GGCTCCAGCCCCACGGTTCTGAAGAAGGCCTTCCAGGCCACGCTCAGGTGGCTCCTGAGC; TCACCCAAGACCCCCGGCTGCTCTGATCTCGGCCCCCTCATCCCGCAGTTCCTCAGAGAG; CTGTTCCCTGTGCTGCAGAAACGCCTGTGCCACCCCTGCTGGGAGGTGAGGGACTCCGCC; CTCGAGTTCCTGACCCAGCTGAGCAGGCACTGGGGAGGACAGGCTGACTTCAGATGCGCA; CTCTTGGCTTCAGAGGTGCCTCAGCTGGCCCTGCAGCTCCTCCAGGACCCTGAGAGTTAT; GTCCGAGCGAGTGCAGTGACCGCCATGGGGCAGCTGTCCAGCCAGGGCCTGCACGCCCCC; ACCAGCCCTGAGCATGCAGAGGCCCGGCAGAGCCTGTTCCTGGAGCTCCTGCACATCCTC; TCCGTAGACTCGGAGGGCTTCCCACGGCGGGCGGTCATGCAAGTCTTCACTGAGTGGCTG; CGGGACGGCCACGCCGACGCGGCCCAGGACACGGAGCAGTTCGTGGCCACTGTGCTGCAG; GCGGCGAGCCNNNACCTGGACTGGGAGGTCCGCGCCCAGGGCCTGGAGCTGGCCCTCGTG; TTCCTGGGCCAGACTTTGGGGCCGCCGCGTACCCACTGCCCCTATGCCGTGGCCCTACCC; GAGGTGGCCCCAGCCCAGCCACTCACCGAGGCACTGAGGGCTCTCTGCCACGTGGGGCTC; TTTGACTTCGCCTTTTGTGCCTTGTTTGACTGCGACCGCCCTGTGGCGCAGAAGTCTTGT; GACCTCCTTCTCTTCCTGAGGGACAAGATTGCTTCCTACAGCAGCCTGCGGGAGGCCAGG; GGCAGCCCCAACACTGCCTCCGCAGAGGCCACCCTGCCGAGGTGGCGGGCGGGTGAGCAG; GCCCAGCCCCCAGGGGACCAGGAGCCTGAGGCTGTGCTGGCCATGCTCAGGTCCCTAGAC; CTGGAGGGCCTGCGGAGCACGCTGGCCGAGAGCAGCGACCACGTGGAGAAGAGTCCCCAG; TCCCTCCTGCAGGACATGCTGGCCACGGGAGGCTTCCTGCAGGGGGACGAGGCCGACTGC; TACTGA; ; Clone variation with respect to NM_152743.3; 271 c=>t;699 g=>a;1931 g=>n;1932 a=>n;1933 g=>n;2388 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152743 unedited; GCACGAGGGGCCTGCGGCCTCACCATGGACCCAGAATGCGCCCAGCTGCTCCCGGCTCTC; TGTGCTGTTCTGGTAGATCCCAGGCAGCCGGTGGCAGATGACACCTGTTTGGAGAAGCTC; CTGGACTGGTTTAAAACGGTCACTGAAGGAGAGTCCAGTGTCGTGCTGCTGCAGGAGCAC; CCCTGCCTGGTGGAGCTGCTGTCCCATGTGCTGAAAGTCCAGGACCTGAGTTCTGGGGTC; CTCTCCTTCTCACTGCGCCTGGCAGGAACCTTCGCAGCCCAGGAAAACTGCTTCTAGTAT; CTTCAGCAGGGGGAGTTACTACCAGGGCTCTTTGGGGAGCCAGGACCCCTCGGCCGAGCA; ACCTGGGCCGTCCCCACCGTGCGCAGCGGCTGGATCCAGGGCCTGCGCTCCCTGGCACAG; CACCCCAGCGCCCTGCGCTTCCTGGCCGACCATGGTGCGGTCGACACCATCTTCTCCCTG; CAGGGAGACTCCAGCCTGTTTGTGGCCTCGGCGGCCAGTCAGCTCCTGGTGCACGTCCTG; GCTTTGTCCATGCGAGGTGGAGCCGAGGGGCAGCCCTGCCTGCCGGNGGGTGACTGGCCC; GCGTGTGCCCAGAAGATCATGGATCACGTTGAAGAGTCCTTGTGCTCCGCGGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152743 unedited; ATAACTGGGTTGTGTCCGCATCGANGTTCNGTTTTAATTTTTTTTTTTTTTGAGTAGAAA; TTTTTCATTTCTTTAATACATCAAACTGTGGGATTTCTTGACCTTGCTTCTCTCCTGGTC; CTGGCTTCCCCAGAGGATCCACCGGGCTGGGCTGGAGCCCTGGGGCTGGCAGTGTCCCAC; AGAAGGACATGGTGCTGCCTCCCTTGGTCCTGATCCCCAGTGGCAGAGTCTGGTTCTGCT; CAGTAGCAGTCGGCCTCGTCCCCCTGCAGGAAGCCTCCCGTGGCCAGCATGTCCTGCAGG; AGGGACTGGGGACTCTTCTCCACGTGGTCGCTGCTCTCGGCCAGCGTGCTCCGCAGGCCC; TCCAGGTCTAGGGACCTGAGCATGGCCAGCACAGCCTCAGGCTCCTGGTCCCCTGGGGGC; TGGGCCTGCTCACCCGCCCGCCACCTCGGCAGGGTGGCCTCTGCGGAGGCAGTGTTGGGG; CTGCCCCTGGCCTCCCGCAGGCTGCTGTAGGAAGCAATCTTGTCCCTCANGAAGAGAAGG; AGGTCACAAGACTTCTGCGCCACAGGGCGGTCGCAGTCAAACAAGGCACAAAAGGCGAAG; TCAAAGAGCCCCACGTGGCAGAGAGCCCTCAGTGCCTCGGTGAGTGGCTGGGCTGGGGCC; ACCTCGGGTAGGGCCACGGCATATGGGCAGTGGGTACGCGGCGGCCCCAAAGTCTGGCCC; CAGGACACGATGGCCAGCTTCAGGCCCTGGGCGCGGACCTNCCAGTCCAGGTCCTGGCTC; GCCGCCTGCAGCCAGTGGCCACGAACTGCTCCGTGTCCTTGGCCGCGTCGGCGTGGCCGT; CCCGCACCACTCAGTGAAGACCTGCATGACCCGCCCNCCGTGGGAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_152743
• Insert Size: 2850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152743.2, NP_689956.1
• RefSeq Size: 2713 bp
• RefSeq ORF: 2466 bp
• Locus ID: 221927
• UniProt ID: Q6PJG6
• Cytogenetics: 7p22.3
• Summary: The protein encoded by this ubiquitously expressed gene interacts with the tumor suppressing BRCA1 (breast cancer 1) protein and and the ATM (ataxia telangiectasia mutated) protein. ATM is thought to be a master controller of cell cycle checkpoint signalling pathways that are required for cellular responses to DNA damage such as double-strand breaks that are induced by ionizing radiation and complexes with BRCA1 in the multi-protein complex BASC (BRAC1-associated genome surveillance complex). The protein encoded by this gene is thought to play a role in the DNA damage pathway regulated by BRCA1 and ATM. [provided by RefSeq, Mar 2012]; Transcript Variant: This variant (2) uses an alternate in-frame splice junction in the 3' end of the coding sequence compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.