LMBR1 (NM_022458) Human Untagged Clone

SKU SC126638

LMBR1 (untagged)-Human limb region 1 homolog (mouse) (LMBR1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: LMBR1
• Synonyms: ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT; ZRS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126638 sequence for NM_022458 edited (data generated by NextGen Sequencing); ATGGAAGGGCAGGACGAGGTGTCGGCGCGGGAGCAGCACTTCCACAGCCAAGTGCGGGAG; TCCACGATATGTTTCCTTCTTTTTGCCATTCTCTACGTTGTTTCCTACTTCATCATCACA; AGATACAAGAGAAAATCAGATGAACAAGAAGATGAAGATGCCATCGTCAACAGGATTTCG; TTGTTTTTGAGCACGTTCACTCTCGCAGTGTCAGCTGGGGCTGTTTTGCTTTTACCCTTC; TCAATCATCAGCAATGAAATCCTGCTTTCTTTTCCTCAGAACTACTATATTCAGTGGCTA; AATGGCTCCCTGATTCATGGTTTGTGGAATCTTGCTTCCCTTTTTTCCAACCTTTGTTTA; TTTGTATTGATGCCCTTTGCCTTTTTCTTTCTGGAATCAGAAGGCTTTGCTGGCCTGAAA; AAGGGAATCCGAGCCCGCATTTTAGAGACTTTGGTCATGCTTCTTCTTCTTGCGTTACTC; ATTCTTGGGATAGTGTGGGTAGCTTCAGCACTCATTGACAACGATGCCGCAAGCATGGAA; TCTTTATATGATCTCTGGGAGTTCTATCTACCCTATTTATATTCCTGTATATCATTGATG; GGATGTTTGTTACTTCTCTTGTGTACACCAGTTGGCCTTTCTCGTATGTTCACAGTGATG; GGTCAGTTGCTAGTGAAGCCAACAATTCTTGAAGACCTGGATGAACAAATTTATATCATT; ACCTTAGAGGAAGAAGCACTCCAGAGACGACTAAATGGGCTGTCTTCATCGGTGGAATAC; AACATAATGGAGTTGGAACAAGAACTTGAAAATGTAAAGACTCTTAAGACAAAATTAGAG; AGGCGAAAAAAGGCTTCAGCATGGGAAAGAAATTTGGTGTATCCCGCTGTTATGGTTCTC; CTTCTTATTGAGACATCCATCTCGGTCCTCTTGGTGGCTTGTAATATTCTTTGCCTATTG; GTTGATGAAACAGCAATGCCAAAAGGAACAAGGGGGCCTGGAATAGGAAATGCCTCTCTT; TCTACGTTTGGTTTTGTGGGAGCTGCGCTTGAAATCATTTTGATTTTCTATCTTATGGTG; TCCTCTGTTGTCGGCTTCTATAGCCTTCGATTTTTTGGAAACTTTACTCCCAAGAAAGAT; GACACAACTATGACAAAGATCATTGGAAATTGTGTGTCCATCTTGGTTTTGAGCTCTGCT; CTGCCTGTGATGTCGAGAACACTGGGAATCACTAGATTTGATCTACTTGGCGACTTTGGA; AGGTTTAATTGGCTGGGAAATTTCTATATTGTATTATCCTACAATTTGCTTTTTGCTATT; GTGACAACATTGTGTCTGGTCCGAAAATTCACCTCTGCAGTTCGAGAAGAACTTTTCAAG; GCCCTAGGGCTTCATAAACTTCACTTACCAAATACTTCAAGGGATTCAGAAACAGCCAAG; CCTTCTGTAAATGGGCATCAGAAAGCACTGTGA; ; Clone variation with respect to NM_022458.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022458 unedited; GTTTTGTAATACGACTCACTATAGGGCGACGCGCNGATTCGGCACGAGGGTCGTGGTCGC; GGTACCTGTTCCAACACGGCTCGCGGGCCCGTGCCGGCTCCGGTCCCCGGCGCGGCTGTC; CGAGCCCCTGCGGCGGGCGGACGATGGTGTGGCGGAGCACGCGGACGCGGGCGGCGCGGC; GGCGGGCATGAAGGAGGATGGAAGGGCAGGACGAGGTGTCGGCGCGGGAGCAGCACTTCC; ACAGCCAAGTGCGGGAGTCCACGATATGTTTCCTTCTTTTTGCCATTCTCTACGTTGTTT; CCTACTTCATCATCACAAGATACAAGAGAAAATCAGATGAACAAGAAGATGAAGATGCCA; TCGTCAACAGGATTTCGTTGTTTTTGAGCACGTTCACTCTCGCAGTGTCAGCTGGGGCTG; TTTTGCTTTTACCCTTCTCAATCATCAGCAATGAAATCCTGCTTTCTTTTCCTCAGAACT; ACTATATTCAGTGGCTAAATGGCTCCCTGATTCATGGTTTGTGGAATCTTGCTTCCCTTT; TTTCCAACCTTTGTTTATTTGTATTGATGCCCTTTGCCTTTTTCTTTCTGGAATCAGAAG; GCTTTGCTGGCCTGAAAAAGGGAATCCGAGCCCCGCATTTTACAGACTTTGGTCATGCTT; CTTCTTCTTGCGTTACTCATTCTTGGGATAGTGTGGGTAGCTTCAGCCCTCATTGACAAC; GATGCCGCAGGCATGGAATCTTTATTGGATCTCCGGGGAGTTTTATCCTACCCTATTTAT; ATTCCTGTAATCATTGAGGGGCACGGTTTGCTCCTCCCCTTGGGCCACCANTTGGCCTTT; CTCTGATGTTACAGTGAAGGGTCCCGTCCCTTATGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022458 unedited; GTTGACATTTGCGATGGCACTTCCAGTCCAGGANAGCACTGGGGCAGGGGTACACAGGGC; ATGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGA; GTTTTTTTTTTTTTTTTTTAATTATTTACAATTTAATAACTGAATGATTATCAGATCGTG; TTATACTGCAAAACTGTTCTTACACCATGAATGCTGATGCTGTGAACTTTGGATGTTAAA; CTGGTAAAAGCTGGAGGCTTCAAATGGCATATGCAAATGTAACCAAAGCCTGAGGACTAT; GAAGAAAGAGGAGTTTCTACCACTCGGCATTTATAGTTTTTATATGCATTGCAGAAGCAT; GGGGAATTAAGTGATCTCTGAAAAAAATGCTTTAAAAAACTGAGTATACATATAGCCTAT; AAGGTAAAAATCCAAGTTACAAGTGTATTAAACACTGGCAACTATGTTATCAGAGGGAAT; ACTGAATTATATATATACATAAACTGTCATTAAACTGTGATTACATGATACTTCCTACCA; CAGCTTATTAGACTGTACAACAGAAGATATTCCTGTGGCTCTCTAAGGCTCCAGGTTTAA; AAGAATTTAGTATTTTCCCCTTGGCTCANAGTCTAATCAGTATCATTCTATAACATAAAT; CAGACACTACGCTTTTTGCTTTTAAGGAAATAAGAAACATAATTGCAACCATTACAGCTC; CAACATACACTGCAAAAATGATATCTACNAGCAGTTGCCATTGCATTTGAAAAATGCATT; TTCTGAAATAGCTTCACTTAGTACCAAGGATGCCTTTCAAGTCAGCTTCTACATTCTGAA; ATAAGTACANTATGGGATTTAAGTAAATCTTTAGAGTCCCGGNATTTTGCCTTTCTAACA; ATTTCAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_022458
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022458.2, NP_071903.2
• RefSeq Size: 2781 bp
• RefSeq ORF: 1473 bp
• Locus ID: 64327
• UniProt ID: Q8WVP7
• Cytogenetics: 7q36.3
• Domains: LMBR1, YdjC
• Protein Families: Transmembrane
• Summary: This gene encodes a member of the LMBR1-like membrane protein family. Another member of this protein family has been shown to be a lipocalin transmembrane receptor. A highly conserved, cis-acting regulatory module for the sonic hedgehog gene is located within an intron of this gene. Consequently, disruption of this genic region can alter sonic hedgehog expression and affect limb patterning, but it is not known if this gene functions directly in limb development. Mutations and chromosomal deletions and rearrangements in this genic region are associated with acheiropody and preaxial polydactyly, which likely result from altered sonic hedgehog expression. [provided by RefSeq, Jul 2008]