TASP1 (NM_017714) Human Untagged Clone

SKU SC126546

TASP1 (untagged)-Human taspase, threonine aspartase, 1 (TASP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TASP1
• Synonyms: C20orf13; dJ585I14.2; SULEHS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126546 sequence for NM_017714 edited (data generated by NextGen Sequencing); ATGACCATGGAGAAGGGGATGAGTTCTGGAGAAGGGCTGCCTTCCAGATCATCTCAGGTT; TCGGCTGGTAAAATAACAGCCAAAGAGTTGGAAACAAAGCAGTCCTATAAAGAGAAACGA; GGAGGCTTTGTGTTGGTGCATGCAGGTGCAGGTTATCATTCTGAATCCAAAGCCAAGGAG; TATAAACATGTATGCAAACGAGCTTGTCAGAAGGCAATTGAAAAGCTGCAGGCCGGTGCT; CTTGCAACTGACGCAGTCACTGCAGCACTGGTGGAACTTGAGGATTCTCCTTTTACAAAT; GCAGGAATGGGATCTAATCTAAATCTGTTAGGTGAAATTGAGTGTGATGCCAGCATAATG; GATGGAAAATCCTTAAATTTTGGAGCAGTTGGAGCACTGAGTGGAATCAAGAACCCAGTC; TCGGTTGCCAACAGACTCTTATGTGAAGGGCAGAAGGGCAAGCTCTCGGCTGGCAGAATT; CCTCCCTGCTTTTTAGTTGGAGAAGGAGCCTACAGATGGGCAGTAGATCATGGAATACCC; TCTTGCCCTCCTAACATCATGACCACAAGATTCAGTTTAGCTGCATTTAAAAGAAACAAG; AGGAAACTAGAGCTGGCAGAAAGGGTGGACACAGATTTTATGCAACTAAAGAAAAGAAGA; CAATCAAGTGAGAAGGAAAATGACTCAGGCACTTTGGACACGGTAGGCGCTGTGGTTGTG; GACCACGAAGGGAATGTTGCTGCTGCTGTCTCCAGTGGAGGCTTGGCCTTGAAACATCCG; GGGAGAGTTGGGCAGGCTGCTCTTTATGGATGTGGCTGCTGGGCTGAAAATACTGGAGCT; CATAACCCCTACTCCACAGCTGTGAGTACCTCAGGATGTGGAGAGCATCTTGTGCGCACC; ATACTGGCTAGAGAATGTTCACATGCTTTACAAGCTGAGGATGCTCACCAAGCCCTGTTG; GAGACTATGCAAAACAAGTTTATCAGTTCACCTTTCCTTGCCAGTGAAGATGGCGTGCTT; GGCGGAGTGATTGTCCTCCGTTCATGCAGATGTTCTGCCGAGCCTGACTCCTCCCAAAAT; AAGCAGACACTTCTAGTGGAATTTCTGTGGAGCCACACGACGGAGAGCATGTGTGTCGGA; TATATGTCAGCCCAGGATGGGAAAGCCAAGACTCACATTTCAAGACTTCCTCCTGGTGCG; GTGGCAGGACAGTCTGTGGCAATCGAAGGTGGGGTGTGCCGCCTGGAGAGCCCAGTGAAC; TGA; ; Clone variation with respect to NM_017714.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017714 unedited; TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAGGCTCGGGGCTGA; AGCGGGGTAATTCCTCTCCTGCAATTACTTTTGGATGGAAGTATGCCCCTTTCTCAGTAG; AAGATGGTAATCTTGGAGAATGACCATGGAGAAGGGGATGAGTTCTGGAGAAGGGCTGCC; TTCCAGATCATCTCAGGTTTCGGCTGGTAAAATAACAGCCAAAGAGTTGGAAACAAAGCA; GTCCTATAAAGAGAAACGAGGAGGCTTTGTGTTGGTGCATGCAGGTGCAGGTTATCATTC; TGAATCCAAAGCCAAGGAGTATAAACATGTATGCAAACGAGCTTGTCAGAAGGCAATTGA; AAAGCTGCAGGCCGGTGCTCTTGCAACTGACGCAGTCACTGCAGCACTGGTGGAACTTGA; GGATTCTCCTTTTACAAATGCAGGAATGGGATCTAATCTAAATCTGTTAGGTGAAATTGA; GTGTGATGCCAGCATAATGGATGGAAAATCCTTAAATTTTGGAGCAGTTGGAGCACTGAG; TGGAATCAAGAACCCAGTCTCGGTTGCCAACAGACTCTTATGTGAAGGGCAGAAGGGCAA; GCTCTCGGCTGGCAGAATTCCTCCCTGCNTTTTAGTTGGAGAAGGAGCCTACAGATGGGC; AGTAGATCATGGAATACCCTCTTGCCCTNNCTACATCATGACCACCNAGATCAGTTTAGC; TGCATTTAAAAGAAACCAGAGGAACTANAGCTGGCAGAAAGGTTGACACANGATTTATGC; ACTAAGAAAAGAGACCATCAGTGAGAAGGAAATGACTCAGCCCTTTGACACCGTAGCGCT; GTGGTGTGGACCACAAGGATGTGCTGTGCTGNCTCCATGGAGCTGGCCTTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_017714
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017714.1, NP_060184.1
• RefSeq Size: 2347 bp
• RefSeq ORF: 1263 bp
• Locus ID: 55617
• UniProt ID: Q9H6P5
• Cytogenetics: 20p12.1
• Domains: Asparaginase_2
• Protein Families: Protease
• Summary: This gene encodes an endopeptidase that cleaves specific substrates following aspartate residues. The encoded protein undergoes posttranslational autoproteolytic processing to generate alpha and beta subunits, which reassemble into the active alpha2-beta2 heterotetramer. It is required to cleave MLL, a protein required for the maintenance of HOX gene expression, and TFIIA, a basal transcription factor. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]