Treacher Collins syndrome protein (TCOF1) (NM_001008657) Human Untagged Clone

SKU SC126432

TCOF1 (untagged)-Human Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: Treacher Collins syndrome protein
• Synonyms: MFD1; TCS; TCS1; treacle
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001008657 edited; AGGTAGCCGGCCGGCCGGGGGTCGCGGGTATGGCCGAGGCCAGGAAGCGGCGGGAGCTAC; TTCCCCTGATCTACCACCATCTGCTGCGGGCTGGCTATGTGCGTGCGGCGCGGGAAGTGA; AGGAGCAGAGCGGCCAGAAGTGTTTCCTGGCTCAGCCCGTAACCCTTCTGGACATCTATA; CACACTGGCAACAAACCTCAGAGCTTGGTCGGAAGCGGAAGGCAGAGGAAGATGCGGCAC; TGCAAGCTAAGAAAACCCGTGTGTCAGACCCCATCAGCACCTCGGAGAGCTCGGAAGAGG; AGGAAGAAGCAGAAGCCGAAACCGCCAAAGCCACCCCAAGACTAGCATCTACCAACTCCT; CAGTCCTGGGGGCGGACTTGCCATCAAGCATGAAAGAAAAAGCCAAGGCAGAGACAGAGA; AAGCTGGCAAGACTGGGAATTCCATGCCACACCCTGCCACTGGGAAGACGGTGGCCAACC; TTCTTTCTGGGAAGTCTCCCAGGAAGTCAGCAGAGCCCTCAGCAAATACTACGTTGGTCT; CAGAAACTGAGGAGGAGGGCAGCGTCCCGGCCTTTGGAGCTGCTGCCAAGCCTGGGATGG; TGTCAGCGGGCCAGGCCGACAGCTCCAGCGAGGACACCTCCAGCTCCAGTGATGAGACAG; ACGTGGAGGGGAAACCCTCAGTAAAACCAGCCCAGGTCAAAGCCTCATCAGTTTCTACTA; AGGAGTCTCCAGCAAGAAAGGCGGCCCCAGCCCCTGGGAAGGTGGGGGATGTGACACCCC; AGGTCAAAGGAGGGGCCCTGCCCCCAGCCAAGAGGGCCAAGAAGCCAGAAGAGGAGTCAG; AGAGTAGTGAGGAGGGATCTGAAAGTGAGGAGGAGGCCCCTGCAGGGACACGAAGCCAGG; TAAAGGCCTCTGAAAAAATTCTCCAGGTCAGAGCTGCCTCAGCCCCTGCCAAGGGGACCC; CTGGGAAAGGGGCTACCCCAGCACCCCCTGGGAAGGCAGGGGCTGTAGCCTCCCAGACCA; AGGCAGGGAAGCCAGAGGAGGACTCAGAGAGCAGCAGCGAGGAGTCATCTGACAGTGAGG; AGGAGACGCCAGCTGCCAAGGCCCTGCTTCAGGCGAAGGCCTCAGGAAAAACCTCTCAGG; TCGGAGCTGCCTCAGCCCCTGCCAAGGAGTCCCCCAGGAAAGGAGCTGCCCCAGCGCCCC; CTGGGAAGACAGGGCCTGCAGTTGCCAAGGCCCAGGCGGGGAAGCGGGAGGAGGACTCGC; AGAGCAGCAGCGAGGAATCGGACAGTGAGGAGGAGGCGCCTGCTCAGGCGAAGCCTTCAG; GGAAGGCCCCCCAGGTCAGAGCCGCCTCGGCCCCTGCCAAGGAGTCCCCCAGGAAAGGGG; CTGCCCCAGCACCTCCTAGGAAAACAGGGCCTGCAGCCGCCCAGGTCCAGGTGGGGAAGC; AGGAGGAGGACTCAAGAAGCAGCAGCGAGGAGTCAGACAGTGACAGAGAGGCACTGGCAG; CCATGAATGCAGCTCAGGTGAAGCCCTTGGGGAAAAGCCCCCAGGTGAAACCTGCCTCTA; CCATGGGCATGGGGCCCTTGGGGAAAGGCGCCGGCCCAGTGCCACCCGGGAAGGTGGGGC; CTGCAACCCCCTCAGCCCAGGTGGGGAAGTGGGAGGAGGACTCAGAGAGCAGTAGTGAGG; AGTCATCAGACAGCAGTGATGGAGAGGTGCCCACAGCTGTGGCCCCGGCTCAGGAAAAGT; CCTTGGGGAACATCCTCCAGGCCAAACCCACCTCCAGTCCTGCCAAGGGGCCCCCTCAGA; AGGCAGGGCCTGTAGCCGTCCAGGTCAAGGCTGAAAAGCCCATGGACAACTCGGAGAGCA; GCGAGGAGTCATCGGACAGTGCGGACAGTGAGGAGGCACCAGCAGCCATGACTGCAGCTC; AGGCAAAACCAGCTCTGAAAATTCCTCAGACCAAGGCCTGCCCAAAGAAAACCAATACCA; CTGCATCTGCCAAGGTCGCCCCTGTGCGAGTGGGCACCCAAGCCCCCCGGAAAGCAGGAA; CTGCGACTTCTCCAGCAGGCTCATCCCCAGCTGTGGCTGGGGGCACCCAGAGACCAGCAG; AGGATTCTTCAAGCAGTGAGGAATCAGATAGTGAGGAAGAGAAGACAGGTCTTGCAGTAA; CCGTGGGACAGGCAAAGTCTGTGGGGAAAGGCCTCCAGGTGAAAGCAGCCTCAGTGCCTG; TCAAGGGGTCCTTGGGGCAAGGGACTGCTCCAGTACTCCCTGGGAAGACGGGGCCTACAG; TCACCCAGGTGAAAGCTGAAAAGCAGGAAGACTCTGAGAGCAGTGAGGAGGAATCAGACA; GTGAGGAAGCAGCTGCATCTCCAGCACAGGTGAAAACCTCAGTAAAGAAAACCCAGGCCA; AAGCCAACCCAGCTGCCGCCAGAGCACCTTCAGCAAAAGGGACAATTTCAGCCCCTGGAA; AAGTTGTCACTGCAGCTGCTCAAGCCAAGCAGAGGTCTCCATCCAAGGTGAAGCCACCAG; TGAGAAACCCCCAGAACAGTACCGTCTTGGCGAGGGGCCCAGCATCTGTGCCATCTGTGG; GGAAGGCCGTGGCTACAGCAGCTCAGGCCCAGACAGGGCCAGAGGAGGACTCAGGGAGCA; GTGAGGAGGAGTCAGACAGTGAGGAGGAGGCGGAGACGCTGGCTCAGGTGAAGCCTTCAG; GGAAGACCCACCAGATCAGAGCTGCCTTGGCTCCTGCCAAGGAGTCCCCCAGGAAAGGGG; CTGCCCCAACACCTCCTGGGAAGACAGGGCCTTCGGCTGCCCAGGCAGGGAAGCAGGATG; ACTCAGGGAGCAGCAGCGAGGAATCAGACAGTGATGGGGAGGCACCGGCAGCTGTGACCT; CTGCCCAGGACCAGGAGTCTTCTTGAATCCCATGAGCTCTGCCCAGGACCCTCTCTCAAG; CACCTGTGGCATACCTTCATGTGCTCCACTGCACCCAGAGCTTAAGCCACAGTCCTGCTC; AGGCTACACCTCCAAGGGTGGGACCAGGCCCACCTTATCTTCCTGTACTCCAGAGGCCCA; AGTCAGCCATGCTGGTGTGGTCCAAGCTTCTGTGTGAACCCTGGCCCTTCCATCAGACAG; CATTACCCTTTGTCCTCCTCTAGCCCCAAGCTCCCAGAAGCTTCTGCCAGGCACCTCAGA; GCACCAGCTCCCCTGTCCCAGGGCCTAACCACAGATCCTGGAGTTCTGCAACCTCCAGAC; CAGAGCGGTCTCATGAACCACCTCCCCTTGAATATCACATACCACATTGTTATCAATTCA; TCAGTAAGGTCAGATGCTGTTTGCACACCTCCAGCAATAGGGTGCTCACTGCCTCTCACT; TTCCAATGGCACAGATTACAAAAGTAAAGAACAACCACCAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001008657 unedited; GGGTTAGATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAGGTAGCC; GGCCGGCCGGGGGTCGCGGGTATGGCCGAGGCCAGGAAGCGGCGGGAGCTACTTCCCCTG; ATCTACCACCATCTGCTGCGGGCTGGCTATGTGCGTGCGGCGCGGGAAGTGAAGGAGCAG; AGCGGCCAGAAGTGTTTCCTGGCTCAGCCCGTAACCCTTCTGGACATCTATACACACTGG; CAACAAACCTCAGAGCTTGGTCGGAAGCGGAAGGCAGAGGAAGATGCGGCACTGCAAGCT; AAGAAAACCCGTGTGTCAGACCCCATCAGCACCTCGGAGAGCTCGGAAGAGGAGGAAGAA; GCAGAAGCCGAAACCGCCAAAGCCACCCCAAGACTAGCATCTACCAACTCCTCAGTCCTG; GGGGCGGACTTGCCATCAAGCATGAAAGAAAAAGCCAAGGCAGAGACAGAGAAAGCTGGC; AAGACTGGGAATTCCATGCCACACCCTGCCACTGGGAAGACGGTGGCCAACCTTCTTTCT; GGGAAGTCTCCCAGGAAGTCAGCAGAGCCCTCAGCAAATACTACGTTGGTCTCAGAAACT; GAGGAGGAGGGCAGCGTCCCGGCCTTTGGAGCTGCTGCCAAGCCTGGGATGGTGTCAGCG; GGCCAGGCCGACAGCTCCAGCGAGGACACCTCCAGCTCCAGTGATGAGACAGACGTGGAG; GGGAAACCCTCAGTAAAACCAGCCCCAGTCAAAGCCTCATCAGTTTCTACTAAGGAGTCT; CCAGCAAGAAAGGCNGCCCCAGCCCCTGGGAGGTGGNGGGATGTGACACCCCCAGTCAAA; GGAGGGGCCCTGCCCCCAGCCAAGAGGTCCAAGNAGCCNNNAGAGGAGTCAGAGAGTATT; GAGGAGGGATCTGG;
• Restriction Sites: Please inquire
• ACCN: NM_001008657
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001008657.1, NP_001008657.1
• RefSeq Size: 3827 bp
• RefSeq ORF: 2877 bp
• Locus ID: 6949
• UniProt ID: Q13428
• Cytogenetics: 5q32-q33.1
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Summary: This gene encodes a nucleolar protein with a LIS1 homology domain. The protein is involved in ribosomal DNA gene transcription through its interaction with upstream binding factor (UBF). Mutations in this gene have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]; Transcript Variant: This variant (3) uses an alternate terminal exon and lacks 10 3' exons, compared to variant 4. The resulting isoform (c) has a significantly shorter and distinct C-terminus, compared to isoform d.