SLC5A12 (NM_178498) Human Untagged Clone

SKU SC126425

SLC5A12 (untagged)-Human solute carrier family 5 (sodium/glucose cotransporter), member 12 (SLC5A12)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SLC5A12
• Synonyms: SMCT2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_178498 edited; CTTTAGGAGAGGAGCAGACAGCTCTTAGCTAGGGTCAGATTTCAAATTCTCATCTCTTGG; TGCCAATACCACCACCAGATTCTTCTTTGAAGTCAACTTTTGAGATCTTCACTAAGTACA; CGTTGGTGTCTGAAGATTCACACGAGTGCCTCTGGTAATCATTTTCTTCAGGGAATCACA; GTCTCTCCTCTCAGCAAAGCATCCACTGTACTGAACTTTGCTTTTGGAAACATCTTCTTC; CTGAGACCTCGTTGAAAGAAACTCTCTGGTGTCATACTTTCCAATATGGAGGTGAAGAAC; TTTGCAGTTTGGGATTATGTTGTATTTGCAGCCCTCTTTTTCATTTCCTCTGGAATTGGG; GTGTTCTTTGCCATTAAGGAGAGAAAAAAGGCAACTTCCCGAGAGTTCCTGGTTGGGGGA; AGGCAAATGAGCTTTGGCCCTGTCGGCTTGTCTCTGACAGCCAGCTTCATGTCAGCTGTC; ACGGTCCTGGGGACCCCTTCTGAAGTCTACCGCTTTGGGGCATCCTTCCTAGTCTTCTTC; ATTGCTTACCTATTTGTCATCCTCTTAACATCAGAGCTCTTTCTCCCTGTGTTCTACAGA; TCTGGTATCACCAGCACTTATGAGTACTTACAACTACGATTCAACAAACCAGTTCGCTAT; GCTGCCACAGTCATCTACATTGTACAGACGATTCTCTACACAGGAGTGGTGGTGTATGCT; CCTGCCCTGGCACTCAATCAAGTGACTGGGTTTGATCTCTGGGGCTCTGTGTTTGCAACA; GGAATTGTTTGCACATTCTACTGTACCCTGGGAGGATTAAAAGCAGTGGTGTGGACAGAT; GCATTTCAGATGGTTGTCATGATTGTGGGCTTCTTAACGGTTCTCATTCAAGGATCAACT; CATGCTGGGGGATTCCACAATGTATTAGAGCAATCAACAAATGGATCTCGACTACATATA; TTTGACTTTGATGTAGATCCTCTCAGGCGACACACTTTTTGGACTATCACAGTGGGAGGA; ACTTTTACTTGGCTCGGAATCTATGGGGTCAATCAATCAACTATTCAGCGATGCATCTCT; TGCAAAACAGAAAAGCATGCTAAGCTTGCCTTGTATTTTAACTTGCTGGGTCTCTGGATC; ATTCTGGTGTGTGCTGTCTTCTCTGGCTTAATCATGTACTCTCACTTTAAAGACTGTGAC; CCTTGGACTTCTGGCATCATCTCAGCACCAGACCAGCTGATGCCGTACTTTGTCATGGAG; ATATTTGCCACAATGCCAGGACTGCCAGGACTTTTTGTGGCTTGTGCCTTCAGTGGAACT; CTGAGCACCGTGGCTTCCAGCATCAATGCCTTGGCAACAGTGACCTTTGAGGATTTTGTC; AAGAGCTGTTTTCCTCATCTCTCCGACAAGCTGAGCACCTGGATCAGTAAAGGCTTATGT; AGGTACAGCTGGTGGACTTTCTATTCCATATCATGAAATGTACATGTGGTAGATGTCAAA; ATCCTGCATGTGCTATTCAGGACACACTTATAAACACAAATGCACTGTGAGATATGTGAA; GAATCCCACACATAGATTCTTACACTTCCAAAGATCCAGCAGGGTATCAGGAAAGAATGA; CTAGCTAGTTTACCTTAACTGCCTTTATTTTCAGATACTCTTCTATACTTACCCTACACT; CTAGACGAGGCTTATCCAACCCACGGCCTGTGGGCCGCATGCGGCCCAGGATGGCTTTGA; ATGCAGCCCAACACAAATTCGTAAACTTTCTTAAAACATTCTGGAATTTTTTTGTAATTT; TTTAAATCTTATCAGCTATCATTAGTGTTTGTGTATTTTATGTGTACTCCAAGGCAACTC; CTTCTTTCAACGTGGCCCAGGGAGGCCAAAAGATTGGACACCCCTGCAAACTAATCACAT; CAATCTCTCTTGATTACACTGTGTGATGGTCATATTAGTCTCTCTACATGGGAAGATGTT; GTGCTTACTATGGCCAGTCACTTTGACAAAGAAAAGCCAAATTCTTTTCTAAAGAATTGT; TCCTTATTACCAAACTCCCAGTAACCCCCTCAAAGAATAATTGCCCCTTGCTTAATCATG; CAATTGCATATTATGTTTATACTCACTTCAGCACTTATCAGAGATAATTGCAGTTTCACA; AGGTTTACTTTTTAAAGTATAAACAAATTATGTATTTACTTACTAATAGAAAATATAGGT; AATTTGGGGAAATTTATGTACATTGTTAAATAAAATTATATCTATCTAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_178498 unedited; NNGGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATCANATCTGGTACCGGT; CCGGAATTCCCGGGATCTTTAGGAGAGGAGCAGACAGCTCTTAGCTAGGGTCAGATTTCA; AATTCTCATCTCTTGGTGCCAATACCACCACCAGATTCTTCTTTGAAGTCAACTTTTGAG; ATCTTCACTAAGTACACGTTGGTGTCTGAAGATTCACACGAGTGCCTCTGGTAATCATTT; TCTTCAGGGAATCACAGTCTCTCCTCTCAGCAAAGCATCCACTGTACTGAACTTTGCTTT; TGGAAACATCTTCTTCCTGAGACCTCGTTGAAAGAAACTCTCTGGTGTCATACTTTCCAA; TATGGAGGTGAAGAACTTTGCAGTTTGGGATTATGTTGTATTTGCAGCCCTCTTTTTCAT; TTCCTCTGGAATTGGGGTGTTCTTTGCCATTAAGGAGAGAAAAAAGGCAACTTCCCGAGA; GTTCCTGGTTGGGGGAAGGCAAATGAGCTTTGGCCCTGTCGGCTTGTCTCTGACAGCCAG; CTTCATGTCAGCTGTCACGGTCCTGGGGACCCCTTCTGAAGTCTACCGCTTTGGGGCATC; CTTCCTAGTCTTCTTCATTGCTTACCTATTTGTCATCCTCTTAACATCAGAGCTCTTTCT; CCCTGTGTTCTACAGATCTGGTATCACCAGCACTTATGAGTACTTACAACTACGATTCAA; CAAACCAGTTCGCTATGCTGCCACAGTCATCTACATTGTACAGACGATTCTCTACACAGG; AGTGGTGGTGTATGCTCCTGCCCTGGCACTCAATCAAGTGACTGGGTTTGATCTCTGGGG; CTCTGTGTTTGCAACAGGAATTGTTTGCACATTCTACTGTACCCTGGGAGGATAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_178498
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_178498.2, NP_848593.1
• RefSeq Size: 2315 bp
• RefSeq ORF: 1191 bp
• Locus ID: 159963
• UniProt ID: Q1EHB4
• Cytogenetics: 11p14.2
• Protein Families: Transmembrane
• Summary: Normal blood lactate is maintained at about 1.5 mM, and little filtered lactate is excreted in urine. Reabsorption of lactate is mediated by the low-affinity Na(+)-coupled lactate transporter SLC5A12 in the initial part of the proximal tubule and by the high-affinity Na(+)-coupled lactate transporter SLC5A8 (MIM 608044) in the distal proximal tubule (Gopal et al., 2007 [PubMed 17692818]).[supplied by OMIM, Dec 2008]