RDHE2 (SDR16C5) (NM_138969) Human Untagged Clone

SKU SC126363

SDR16C5 (untagged)-Human short chain dehydrogenase/reductase family 16C, member 5 (SDR16C5)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RDHE2
• Synonyms: EPHD-2; RDH#2; RDH-E2; RDHE2; retSDR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_138969 edited; CCGGCTTCTCCGCGGACAGCTAGGGAGAGTGTCCTGGGTGTCAGCCAGAACATGTCTTTC; AACCTGCAATCATCAAAGAAACTGTTCATTTTCTTAGGAAAATCACTGTTTAGTCTTCTG; GAGGCTATGATTTTTGCCTTACTCCCAAAGCCACGGAAGAACGTTGCTGGTGAAATAGTC; CTCATCACAGGTGCTGGAAGTGGACTCGGAAGGCTCTTAGCCTTGCAGTTTGCCCGGCTG; GGATCTGTTCTTGTTCTCTGGGATATCAATAAGGAGGGGAATGAGGAAACATGTAAGATG; GCTCGGGAAGCTGGAGCCACAAGAGTGCACGCCTATACCTGCGATTGCAGCCAAAAGGAA; GGAGTGTATAGAGTAGCCGACCAGGTTAAAAAAGAAGTCGGCGATGTTTCCATCCTAATC; AACAATGCCGGAATCGTAACAGGCAAAAAGTTCCTTGACTGTCCAGATGAGCTTATGGAA; AAGTCATTTGATGTGAATTTCAAAGCACATTTATGGACTTATAAAGCCTTTCTACCTGCT; ATGATTGCTAATGACCATGGACATTTGGTTTGCATTTCAAGTTCAGCTGGATTAAGTGGA; GTAAATGGGCTGGCAGATTACTGTGCAAGTAAATTTGCAGCCTTTGGGTTTGCTGAATCT; GTATTTGTAGAAACATTTGTCCAAAAACAAAAGGGGATCAAAACCACGATTGTGTGCCCC; TTTTTTATAAAAACTGGAATGTTTGAAGGTTGTACTACAGGCTGTCCTTCTCTGTTGCCA; ATTCTGGAACCAAAATATGCAGTTGAAAAAATAGTAGAAGCTATTCTACAAGAAAAAATG; TACTTGTATATGCCAAAGTTGTTATACTTCATGATGTTTCTTAAAAGCTTTTTGCCCCTC; AAGACAGGACTGCTTATAGCTGACTATTTGGGCATCCTTCATGCAATGGATGGCTTTGTT; GACCAAAAGAAGAAGCTCTAAAGACCAACTCTATGGCTAAGGTCATCTGATACACAGTGT; TACATAATGCGTACTTCAATGAAGAAAAGTATTTTTGTCTGACAGTGGAATATATCTGGA; GACCACAAGTACCACTCCTATTCTGTTATCTGGACTAGAATTTTCAATCAATGTGTTTGA; AAATAATGTTGCTATCACCTATTTGGTTGAGTTTTGGTTTTTTCTTTTTCTTTTTTTTTC; CAAAAATAAAGACAGCCCATTTTTGTCATTTCCATTACCACAGATGTAAGAAAGTGATAA; GCCATGGCCATGAGCTGCCCTGAGCTTTGGTCGTTGGGGAGGTGGCTCAAATGGAGCCAG; CGGCTGTGGTTGACCTGCCTTGGGGAGCAGGGCTGAGGGGCGAAGAGGACATGTGGATAT; CCAGCCAGAATGCCCTCTCTCCAGAGGACACACAGCTCTGGATTTCTACCCCCTTGCTAT; CCTCTCTGGCCATTTTGAACCTGTACCACCTAATGTAGAAAAGGCCCGCCTCCAGTGCCC; TTCTTTCCAAAACAGCTACACAAATGCTTCTGAATGCCCTGGATACGCAGACCGAGTGGC; AGTCTGGCTAAAGGCCCATCCAGCACTATTGTGCCCAAGAGCCTTACCGGGCATTCCCTG; AAGCCCCCTCATAGGCAGTGATCCTAGACCAAACTCTTAAATAGAATCACTTTTGTTTCC; TAGAAGAGATCAAAGAGTGGTTGCTTCTTACTTTGAAGATGAAATTATACCAAGTTAGGT; TTTTTGCTATCTATTGATCCAAAAAATATATTCGTGCAATTAAAGTTAAGTGTTCAACTT; AGTATTTCAAGTTGGGATTCAGGTTGAGGCATTTTCCTGCAGAATGGGCAATTTTATGCA; TTTATATTCATCAGTGCTGCCCTCACTTTGGATCTATTGTATTCTTACAGTTTAATCAAC; TGGCAAAGTGCTTTCATGCTTTGGAGATTAACTATAAAAGCAAGATTAAGAAAAATATGT; TAATTACCATATCCCTGGAACTCCTTCTCAGGCTGGTGGGGGCAGCCTTGCAAGAGAAAC; AGCAGCCTTTGTCTCTTCCCTCTTGTGGGGAGCAAGGAGGGGATGAGCGGTACCTTGGGA; GACCCGGTAAAAGTCTAAAGAATTGAGAATTTGACTATCTTGGGGGTAAAATGCTGTCAT; AGGGGCAGCAGTCTCAGGGGGTGTAAACTCTGGAATCCACTATGGTTTACCTTGAGTGGC; CTGCACGAACATGACAGCCCTGATCTGTATTTCCTTTCCATTCAGAAAACCTTAAAAAAA; AAAAACTTGTTTTATGATAATATTATAACAATCATAACTAACATTTACTGTGCCCGGCAG; CAAATTAAGTATTTGACAAACATTATTTCATTAAACGAGACTGTCAATCATTACTCAAAG; AATGATGAAGGTATTTGGTGTATGCCAAAGAATAAAGGCGTTTTATTTCACAGTGACATC; TTATTCCACTGTAGATGTATACTGAGAGGATCAAAGGCCCTCTCAGAGTCACCCACTGAT; GTTCCAAATCCTTGGTTAACAAGGTTAATTAAGAGAGAAGCACAATATTGCTGTAATATT; ACAATTCCAGAAGCGTATCTTCTAGGGTCCTGTCTATTTAGTTAAAGAATAAAGCTCAGA; CTCTTCGGGGATTCAGTACAACAAAGGTCCAGGAGCACTGCAGGCATCCTCCACATCATA; ACAGCATTAATAGCAATTATTTCTAATTGGAGTGCATCTTGGCTGGACTGCGACAAAGGC; CTGAAATTCAATTGTGGTTTTAAAATACCCATAAAATTGTCTAAACAAATTCAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138969 unedited; GGTCATAATTGTATACCATCATATAGGCGGCCGCGTAATCCCGGGATCCGGCTTCTCCGC; GGACAGCTAGGGAGAGTGTCCTGGGTGTCAGCCAGAACATGTCTTTCAACCTGCAATCAT; CAAAGAAACTGTTCATTTTCTTAGGAAAATCACTGTTTAGTCTTCTGGAGGCTATGATTT; TTGCCTTACTCCCAAAGCCACGGAAGAACGTTGCTGGTGAAATAGTCCTCATCACAGGTG; CTGGAAGTGGACTCGGAAGGCTCTTAGCCTTGCAGTTTGCCCGGCTGGGATCTGTTCTTG; TTCTCTGGGATATCAATAAGGAGGGGAATGAGGAAACATGTAAGATGGCTCGGGAAGCTG; GAGCCACAAGAGTGCACGCCTATACCTGCGATTGCAGCCAAAAGGAAGGAGTGTATAGAG; TAGCCGACCAGGTTAAAAAAGAAGTCGGCGATGTTTCCATCCTAATCAACAATGCCGGAA; TCGTAACAGGCAAAAAGTTCCTTGACTGTCCAGATGAGCTTATGGAAAAGTCATTTGATG; TGAATTTCAAAGCACATTTATGGACTTATAAAGCCTTTCTACCTGCTATGATTGCTAATG; ACCATGGACATTTGGTTTGCATTTCAAGTTCAGCTGGATTAAGTGGAGTAAATGGGCTGG; CAGATTACTGTGCAAGTAAATTTGCAGCCTTTGGGTTTGCTGAATCTGTATTTGTAGAAA; CATTTGTCCAAAAACAAAAGGGGATCAAAACCACGATTGTGTGCCCCTTTTNTATAAAAA; CTGGAATGTTTGAAGGTTGTACTACAGGCTGTCCTTCTCTGTTGCCAATTCTGGAACCAA; ATATGCAGTTGAAAAATAGTAGAAGCTATTCTACAAGAAAAATGTACTTGTATATGCCAA; AGTTGNTATACTTCATGATGTTTCTTAAAAGCTTT;
• Restriction Sites: Please inquire
• ACCN: NM_138969
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_138969.1, NP_620419.1
• RefSeq Size: 2297 bp
• RefSeq ORF: 696 bp
• Locus ID: 195814
• UniProt ID: Q8N3Y7
• Cytogenetics: 8q12.1
• Protein Families: Druggable Genome
• Summary: This gene encodes a member of the short-chain alcohol dehydrogenase/reductase superfamily of proteins and is involved in the oxidation of retinol to retinaldehyde. The encoded protein is associated with the endoplasmic reticulum and is predicted to contain three transmembrane helices, suggesting that it is an integral membrane protein. It recognizes all-trans-retinol and all-trans-retinaldehyde as substrates and exhibits a strong preference for NAD(+)/NADH as cofactors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]; Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region compared to variant 1. It encodes isoform 2, which is shorter than and has a distinct C-terminus compared to isoform 1.