TOP1MT (NM_052963) Human Untagged Clone

SKU SC126339

TOP1MT (untagged)-Human topoisomerase (DNA) I, mitochondrial (TOP1MT), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: TOP1MT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_052963 edited; GGCAGATGCGCGTGGTGCGGCTGCTGCGGCTCCGGGCGGCTCTGACGCTGCTCGGGGAGG; TCCCCCGCCGCCCGGCCTCCCGGGGTGTCCCGGGCTCGCGCAGGACGCAGAAGGGCAGTG; GAGCCAGGTGGGAGAAGGAGAAGCACGAAGACGGGGTGAAGTGGAGACAGCTGGAGCACA; AGGGCCCGTACTTCGCACCCCCATACGAGCCCCTTCCCGACGGAGTGCGTTTCTTCTATG; AAGGAAGGCCTGTGAGATTGAGCGTGGCAGCGGAGGAGGTCGCCACTTTTTATGGGAGGA; TGTTAGATCATGAATACACAACAAAGGAGGTTTTCCGGAAGAACTTCTTCAATGACTGGC; GAAAGGAAATGGCGGTGGAAGAGAGGGAAGTCATCAAGAGCCTGGACAAGTGTGACTTCA; CGGAGATCCACAGATACTTTGTGGACAAGGCCGCAGCCCGGAAAGTCCTGAGCAGGGAGG; AGAAGCAGAAGCTAAAAGAAGAGGCAGAAAAACTTCAGCAAGAGTTCGGCTACTGTATTT; TAGATGGTCACCAAGAAAAAATAGGCAACTTCAAGATTGAGCCGCCTGGCTTGTTCCGTG; GCCGTGGCGACCATCCCAAGATGGGGATGCTGAAGAGAAGGATCACGCCAGAGGATGTGG; TTATCAACTGCAGCAGGGACTCGAAGATCCCCGAGCCGCCGGCGGGGCACCAGTGGAAGG; AGGTGCGCTCCGATAACACCGTCACGTGGCTGGCAGCTTGGACCGAGAGCATTCAGAACT; CCATCAAGTACATCATGCTGAACCCTTGCTCGAAGCTGAAGGGGGAGACAGCTTGGCAGA; AGTTTGAAACAGCTCGACGCCTGCGGGGATTTGTGGACGAGATCCGCTCCCAGTACCGGG; CTGACTGGAAGTCTCGGGAAATGAAGACGAGACAGCGGGCGGTGGCCCTGTATTTCATCG; ATAAGCTGGCACTGAGAGCAGGAAATGAGAAGGAGGACGGTGAGGCGGCCGACACCGTGG; GCTGCTGTTCCCTCCGGGTGGAGCACGTCCAGCTGCACCCGGAGGCCGATGGCTGCCAAC; ACGTGGTGGAATTTGACTTCCTGGGGAAGGACTGCATCCGCTACTACAACAGAGTGCCGG; TGGAGAAGCCGGTGTACAAGAACTTACAGCTCTTTATGGAGAACAAGGACCCCCGGGACG; ACCTCTTCGACAGGCTGACCACGACCAGCCTGAACAAGCACCTCCAGGAGCTGATGGACG; GGCTGACGGCCAAGGTGTTCCGGACCTACAACGCCTCCATCACTCTGCAGGAGCAGCTGC; GGGCCCTGACGCGCGCCGAGGACAGCATAGCAGCTAAGATCTTATCCTACAACCGAGCCA; ACCGAGTCGTGGCCATTCTCTGCAACCATCAGCGAGCAACCCCCAGTACGTTCGAGAAGT; CGATGCAGAATCTCCAGACGAAGATCCAGGCAAAGAAGGAGCAGGTGGCTGAGGCCAGGG; CAGAGCTGAGGAGGGCGAGGGCTGAGCACAAAGCCCAAGGGGATGGCAAGTCCAGGAGTG; TCCTGGAGAAGAAGAGGCGGCTCCTGGAGAAGCTGCAGGAGCAGCTGGCGCAGCTGAGTG; TGCAGGCCACGGACAAGGAGGAGAACAAGCAGGTGGCCCTGGGCACGTCCAAGCTCAACT; ACCTGGACCCCAGGATCAGCATTGCCTGGTGCAAGCGGTTCAGGGTGCCAGTGGAGAAGA; TCTACAGCAAAACACAGCGGGAGAGGTTCGCCTGGGCTCTCGCCATGGCAGGAGAAGACT; TTGAATTCTAACGACGAGCCGTGTTGAAACTTCTTTTGTATGTGTGTGTGTTTTTTTCAC; TATTAAAGCAGTACTGGGGAATTTTGTACAATAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_052963 unedited; TTTAGTCAGAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGCAGAT; GCGCGTGGTGCGGCTGCTGCGGCTCCGGGCGGCTCTGACGCTGCTCGGGGAGGTCCCCCG; CCGCCCGGCCTCCCGGGGTGTCCCGGGCTCGCGCAGGACGCAAAATGGCAGTGGAGCCAG; GTGGGAGAAGGAGAAGCACGAATACGGGGTGAAGTGGAGACAGCTGGAGCACAAGGGCCC; GTACTTCGCACCCCCATACGAGCCCCTTCCCGACGGAGTGCGTTTCTTCTATGAAGGAAG; GCCTGTGAGATTGAGCGTGGCAACGGAGGAGGTCGCCACTTTTTATGGGAGGATGTTAGA; TCATGAATACACAACAAAGGAGGTTTTCCGGAAGAACTTCTTCAATGACTGGCGAAAGGA; AATGGCGGTGGAAGAGAGGGAAGTCATCAAGAGCCTGGACAAGTGTGACTTCACGGAGAT; CCACAGATACTTTGTGGACAAGGCCGCAGCCCGGAAAGTCCTGAGCAGGGAGGAGAAGCA; CAAGCTAAAAGAAGAGGCAGAAAAACTTCAGCAAGAGTTCGGCTACTGTATTTTAGATGG; TCACCAAGAAAAAATAGGCAACTTCAAGATTGAGCCGCCTGGCTTGTTCCGTGGCCGTGG; CGACCATCCCAAGATGGGGATGCTGAAGAGTAGGATCACGCCAGAGGATGTGGTTATCAA; CTGCAGCAGGCACTCGAAGATCCCCGAGCCGCCGGCGGCGCACCACTGGAAGGGAGTGCG; CTCCGATAACACCGTCACGTGGCTGGCAGCTTGGACCGAGAGCATTCAGGACTCCATCAA; GTACATCATGCTGAACCCTTGCTCGAAGCTGAAGGGGGAGACAGCTTGGCANAAGTTTGA; ACAGCTCGAGCC;
• Restriction Sites: Please inquire
• ACCN: NM_052963
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_052963.1, NP_443195.1
• RefSeq Size: 1923 bp
• RefSeq ORF: 1806 bp
• Locus ID: 116447
• UniProt ID: Q969P6
• Cytogenetics: 8q24.3
• Protein Families: Druggable Genome
• Summary: This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]; Transcript Variant: This variant (1) encodes the longer isoform (1).