PRIM1 (NM_000946) Human Untagged Clone

SKU SC126303

PRIM1 (untagged)-Human primase, DNA, polypeptide 1 (49kDa) (PRIM1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PRIM1
• Synonyms: p49
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000946 edited; CCTGTGGTAATCCTTACCGTGGCGAGTTCCGCGCTCAATGGAGACGTTTGACCCCACCGA; GCTGCCCGAGCTGCTTAAACTTTATTACCGGAGGCTCTTTCCCTACTCTCAGTACTATCG; CTGGCTCAACTACGGTGGAGTGATAAAGAATTACTTTCAACACCGTGAATTTTCATTCAC; ATTGAAAGATGATATTTACATTCGCTACCAATCCTTCAACAACCAGAGTGATCTGGAAAA; GGAGATGCAGAAAATGAATCCATACAAGATTGATATAGGCGCAGTATATTCTCACAGACC; CAATCAACACAATACAGTGAAGCTGGGAGCTTTCCAGGCTCAGGAAAAAGAACTGGTATT; TGACATTGACATGACAGACTATGACGATGTGAGGAGATGTTGTAGTTCTGCAGACATATG; TCCTAAGTGCTGGACCCTCATGACAATGGCCATACGCATCATTGACAGAGCATTGAAGGA; GGACTTTGGATTTAAGCATCGTCTCTGGGTATATTCTGGAAGGAGAGGTGTTCATTGTTG; GGTCTGTGATGAATCAGTTAGAAAACTGTCTTCTGCAGTACGTTCTGGGATAGTTGAGTA; TTTGAGCCTTGTAAAGGGTGGTCAAGACGTTAAAAAGAAAGTTCACCTAAGTGAAAAAAT; TCACCCTTTTATCAGAAAATCTATAAACATAATAAAAAAATACTTTGAAGAATATGCCTT; GGTTAATCAAGATATTCTCGAAAATAAAGAAAGCTGGGATAAGATTTTAGCCCTTGTTCC; TGAAACAATTCATGATGAACTTCAACAAAGCTTCCAAAAGTCTCACAATTCACTTCAGCG; TTGGGAGCACTTGAAGAAAGTAGCCAGCAGATATCAGAATAACATCAAAAATGACAAATA; TGGACCCTGGCTGGAGTGGGAGATTATGCTCCAGTACTGTTTTCCACGGCTGGATATCAA; TGTCAGCAAAGGAATCAATCATCTACTGAAGAGCCCTTTTAGTGTTCATCCTAAAACAGG; TCGCATATCTGTGCCTATTGATTTGCAGAAAGTGGACCAGTTTGATCCATTTACTGTTCC; GACCATAAGCTTCATCTGCCGTGAATTGGATGCCATTTCCACTAATGAAGAGGAAAAAGA; GGAGAATGAAGCTGAATCTGATGTCAAACATAGAACCAGAGATTATAAGAAGACCAGTCT; AGCACCTTATGTGAAAGTTTTTGAACATTTTCTTGAAAATCTGGATAAATCCCGAAAAGG; AGAACTTCTTAAGAAGAGTGATTTACAAAAAGATTTCTGAAGACAGAGCTCCTCAAACCA; TTGTGGATATCTTCTGCCTTCAACCACAGATCAAATACTTCAAGAGCCATTTAATAAATA; TGGCAGAACTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000946 unedited; TCAGGATTTGTATACGACTGCACTATAGGCGGCGCGCGAATTCGCCATTACGGCCGGGGC; CTGTGGTAATCCTTACCGTGGCGAGTTCCGCGCTCAATGGAGACGTTTGACCCCACCGAG; CTGCCCGAGCTGCTTAAACTTTATTACCGGAGGCTCTTTCCCTACTCTCAGTACTATCGC; TGGCTCAACTACGGTGGAGTGATAAAGAATTACTTTCAACACCGTGAATTTTCATTCACA; TGGGGAAGATGATATTTACATTCGCTACCAATCCTTCAACAACCAGAGTGATCTGGAAAA; GGAGATGCAGAAAATGAATCCATACAAGATTGATATAGGCGCAGTATATTCTCACAGACC; CAATCAACACAATACAGTGAAGCTGGGAGCTTTCCAGGCTCAGGAAAAAGAACTGGTATT; TGACATTGACATGACAGACTATGACGATGTGAGGAGATGTTGTANTTCTGCAAACATATG; TCCTAAGTGCTGGGACCCTCATGACAATGGCCATACGCATCATTGACAGAGCATTGAAGG; AGGACTTTGGATTTAAGCATCGGCTCTGGGTATATTCTGGAAGAGAGGTGTTCATTGGTN; GGTCTGTGATGAATCAGTTAGAAAACTGTGCTCTGCAGTACTTTCTGGGATAGTTGAGTA; TTTGAGCCTTGGAAAGGTGGGCAAGACGTAAAAAGAAAGTTACCTAGTGAAAAAATCCCC; CCTTTATCGGANATCTTTAACCTATAAAAAATCCTTTGAGAATATGCTTTGGTAATAAGA; ATTCTCCAAATAAGAAACGTGGGTAGGATTTACCCCTGGGCCTGAAACATTCATGAGGAA; TTCAACAAACTCCCAAAGCTAAAATTCCTTCACGGTTGGGGCCTTAAAAAATGCCCCCGA; ATTCAAAACCTCCAAATAACAATGGCGCCGGCGGGG;
• Restriction Sites: Please inquire
• ACCN: NM_000946
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000946.2, NP_000937.1
• RefSeq Size: 1471 bp
• RefSeq ORF: 1263 bp
• Locus ID: 5557
• UniProt ID: P49642
• Cytogenetics: 12q13.3
• Protein Families: Druggable Genome
• Protein Pathways: DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• Summary: The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008]