ETFBKMT (NM_173802) Human Untagged Clone

SKU SC126245

METTL20 (untagged)-Human methyltransferase like 20 (METTL20), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ETFBKMT
• Synonyms: C12orf72; ETFB-KMT; METTL20
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_173802 edited; GCCGCGAGGGGCCGCCGTCCGTCCCGCCCCCTGCCGGGCCGCGGCGCTCTGCTAGAGCGT; GGCTCAGTTTTGGTAGCGGGACCCCGGAAATGTAAAGCATGAGCGGGAACAGTCGCCAGG; GCGAGTGACAGGCGAGCCCTATGCTTTACTTCCGAGGGCGGTGTCAGCAAAGCTAGAGTC; AGAGGTTCCGGTTGAGATCAAGTTGGGAGACACACCCTTGTTCATTCTCCTTGAGAAGCA; GCTATTATCAACAGAACATTGACAGAACCTGTGTTTGGGGAAAGGACTGATGGCTTTGAG; TCTAGGTTGGAAAGCACACAGGAACCACTGTGGTCTCCTCTTGCAGGCTCTGCGAAGCAG; TGGTCTTCTCTTGTTTCCCTGTGGCCAGTGTCCCTGGAGAGGAGCTGGAAGCTTTTTGGA; CCCTGAGATAAAGGCTTTCCTGGAGGAGAACACTGAAGTCACCAGCAGTGGTAGCCTCAC; CCCTGAAATCCAGTTGCGGCTTTTGACCCCCAGATGCAAATTCTGGTGGGAGAGAGCTGA; CCTGTGGCCCCACAGTGATCCTTACTGGGCAATCTACTGGCCAGGAGGCCAAGCCCTGTC; TAGGTATCTTTTGGATAATCCTGATGTTGTCAGAGGAAAATCTGTATTAGATCTTGGGAG; TGGATGTGGAGCTACAGCTATTGCTGCTAAGATGAGTGGGGCATCAAGGATCTTGGCCAA; TGACATAGACCCTATTGCAGGAATGGCTATTACACTAAATTGTGAATTGAACAGACTGAA; TCCTTTTCCTATTTTAATCCAAAACATTTTGAATTTGGAACAAGATAAGTGGGACCTTGT; TGTTCTTGGCGATATGTTTTATGATGAAGACCTTGCAGATAGTCTTCATCAGTGGCTGAA; GAAGTGCTTCTGGACCTATAGAACTCGAGTACTGATTGGTGACCCTGGGCGGCCCCAGTT; CAGTGGACACAGCATTCAGCATCACCTGCACAAAGTGGTAGAATATTCACTTTTGGAGTC; TACTAGGCAGGAAAACAGTGGACTGACAACAAGCACAGTGTGGGGTTTTCAGCCTTGAGT; TGTCAAAGTGCTTCCAAGTATGAATATAGTAATGTTTGGATCTGACTCTAAAAGTTTTCT; CTATAGGAGATACTCTCCAGTTTAATGAATGTAATTCTTGTTAAATGGAAAATCTTGTTT; TTAAAATATGAAGGTTTAGAGTTTTGTTTACTTTTGTCATGTAACTGGTTCTGTATTTCT; ATGCATGCCAATTATACACATCTCTATCTGCATTTTTTTTTCTATTTTAACCGCTGAAGG; AATATGATTATACAATGCCATACTCAATGGTGGCAGCATTTAATTTAAGTAATGGAGAAG; AATTAAACATTGTAGCTGGATCTGTTTCTGTAGTTGAGGACAACTGTGATGTAACTTTGA; ATTAAACAAACAAAACAAAACAGAAGAAGACTATATATATATTTTTTCTTTTTTTAGAGA; CAGTCTCACTTTGCTCCCAGGCTGGAATGCAGTGGCTATTCAGAGGCTTAATCATTGGGC; ACTGCAGCCTTGAACTCTGGGCTTTTTTTTTTTTTGAGATGGAGTCTCAGCTCTGTCGTC; CAGGCTGGAGTGCAGTGACTCATGTCTCGGCTCACTGTAGCCTCCACCTCCCCCAATCAA; GCGATTGTCCTGTCTCAGCCTCCAGAGTAGCTGGGATTTTAGGTGTGCACCACCATGCCT; GGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTTGCCATGTTGGCCAGGCCGGTCTCA; AACTCCTGACCTCAGGTGATCCACCCGCCTTGGCCTCCCAAAGTGCTAGGATTACAGGTG; TGAGCCACCACGCCAGCCTGACGATGTTTTTTACTTCTATCACTTCCTTTTGATTTTTTC; TTATAGCTTCTATCTCTGCTTACGTCACCCATCATTCTTGCGTGTTGTATGCTTTTCGCA; TTAGAGCCCTTTGCATGTTAATCAGTTATTTTAAATTTATGATCTGGTAATTCTAAAATG; TCTGCCATAACTGAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173802 unedited; NNNGGTAGTTCTTGATTTGTNATACGACTTACTATAGGCGGCCGCGGATTCANATCTGGT; ACCGGTCCGGAATTCCCGGGATGCCGCGAGGGGCCGCCGTCCGTCCCGCCCCCTGCCGGG; CCGCGGCGCTCTGCTAGAGCGTGGCTCAGTTTTGGTAGCGGGACCCCGGAAATGTAAAGC; ATGAGCGGGAACAGTCGCCAGGGCGAGTGACAGGCGAGCCCTATGCTTTACTTCCGAGGG; CGGTGTCAGCGAAGCTAGAGTCAGAGGTTCCGGTTGAGATCAAGTTGGGAGACACACCCT; TGTTCATTCTCCTTGAGAAGCAGCTATTATCAACAGAACATTGACAGAACCTGTGTTTGG; GGAAAGGACTGATGGCTTTGAGTCTAGGTTGGAAAGCACACAGGAACCACTGTGGTCTCC; TCTTGCAGGCTCTGCGAAGCAGTGGTCTTCTCTTGTTTCCCTGTGGCCAGTGTCCCTGGA; GAGGAGCTGGAAGCTTTTTGGACCCTGAGATAAAGGCTTTCCTGGAGGAGAACACTGAAG; TCACCAGCAGTGGTAGCCTCACCCCTGAAATCCAGTTGCGGCTTTTGACCCCCAGATGCA; AATTCTGGTGGGAGAGAGCTGACCTGTGGCCCCACAGTGATCCTTACTGGGCAATCTACT; GGCCAGGAGGCCAAGCCCTGTCTAGGTATCTTTTGGATAATCCTGATGTTGTCAGAGGAA; AATCTGTATTAGATCTTGGGAGTGGATGTGGAGCTACAGCTATTGCTGCTAAGATGAGTG; GGGCATCAAGGATCTTGGCCAATGACATAGACCCTATTGCAGGAATGGCTATTACACTAA; ATTGTGAATTGAACAGACTGAATCCTTTTTCCTAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_173802
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_173802.2, NP_776163.1
• RefSeq Size: 2078 bp
• RefSeq ORF: 789 bp
• Locus ID: 254013
• UniProt ID: Q8IXQ9
• Cytogenetics: 12p11.21
• Protein Families: Druggable Genome
• Summary: Protein-lysine methyltransferase that selectively trimethylates the flavoprotein ETFB in mitochondria (PubMed:25023281, PubMed:25416781). Thereby, may negatively regulate the function of ETFB in electron transfer from Acyl-CoA dehydrogenases to the main respiratory chain (PubMed:25416781).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.