TRMT6 (NM_015939) Human Untagged Clone

CAT#: SC126188

TRMT6 (untagged)-Human tRNA methyltransferase 6 homolog (S. cerevisiae) (TRMT6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRMT6
• Synonyms: CGI-09; GCD10; Gcd10p
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015939 edited
  GTGAGGCTGGCGGTGGCGACAACCGAGGAGGAGGGGCGGGACGCCGTGGAGCACGGCGAC
  CGGCTGAGCGTCATGGAGGGCTCAGGGGAGCAGCCGGGCCCACAACCACAGCATCCCGGA
  GACCACCGCATCCGCGACGGCGACTTCGTGGTGCTGAAACGTGAAGATGTGTTTAAAGCA
  GTACAAGTCCAGCGGAGAAAAAAAGTAACTTTCGAAAAACAGTGGTTCTACCTGGATAAC
  GTCATTGGCCATAGTTATGGAACTGCATTTGAAGTGACCAGTGGAGGAAGTCTACAGCCC
  AAGAAGAAGAGGGAAGAGCCTACTGCAGAGACTAAAGAAGCGGGCACTGATAATCGAAAT
  ATAGTTGATGATGGGAAATCTCAGAAACTTACTCAAGATGACATAAAAGCTTTGAAGGAC
  AAGGGCATTAAAGGAGAGGAAATAGTTCAGCAGTTAATTGAAAATAGTACAACATTCCGA
  GACAAGACAGAATTTGCCCAAGATAAATATATTAAAAAGAAGAAAAAAAAATATGAAGCC
  ATCATTACTGTTGTGAAGCCATCCACCCGTATTCTTTCAATTATGTATTATGCAAGAGAA
  CCTGGAAAAATTAACCACATGAGATACGATACACTAGCCCAGATGTTGACGTTGGGAAAT
  ATCCGTGCTGGCAACAAAATGATTGTGATGGAAACGTGTGCAGGCTTGGTGCTGGGTGCA
  ATGATGGAACGAATGGGAGGTTTTGGCTCCATTATTCAGCTATACCCTGGAGGAGGACCT
  GTTCGGGCAGCAACAGCATGTTTTGGATTTCCCAAATCTTTTCTCAGTGGTCTTTATGAA
  TTCCCTCTCAACAAAGTGGACAGTCTTCTACATGGAACATTTTCTGCCAAGATGTTATCT
  TCAGAGCCAAAAGACAGTGCTTTGGTTGAAGAAAGTAATGGCACACTGGAGGAAAAACAG
  GCTTCTGAACAAGAGAATGAAGACAGCATGGCAGAGGCCCCAGAGAGCAACCACCCAGAA
  GACCAGGAAACAATGGAAACAATTTCTCAAGATCCAGAACATAAGGGGCCTAAAGAGAGA
  GGAAGCAAAAAAGATTATATTCAGGAAAAACAGAGGAGACAAGAAGAGCAGAGGAAAAGA
  CATTTAGAGGCTGCCGCTCTGCTGAGTGAAAGAAACGCAGATGGTTTAATTGTAGCTAGT
  CGTTTCCACCCCACTCCCCTGCTGCTGTCTTTGCTGGACTTTGTGGCCCCTTCAAGGCCG
  TTTGTGGTCTACTGTCAGTACAAAGAGCCTCTGTTGGAATGCTACACAAAACTGCGGGAG
  AGGGGAGGGGTCATCAACCTCAGGCTGTCTGAAACCTGGCTCAGAAATTATCAGGTTTTG
  CCAGATCGAAGTCATCCTAAACTGCTGATGAGTGGAGGTGGGGGTTATCTTCTCTCCGGC
  TTCACCGTTGCCATGGACAACCTTAAAGCAGACACCAGCCTCAAATCTAATGCAAGCACT
  TTAGAATCACACGAGACTGAGGAGCCTGCAGCTAAAAAACGAAAATGCCCAGAGTCTGAC
  TCTTAACCCTTTTGAGAATTGCTCTGAATTTTGTTCTCAGGCATTATACAGTTAGTAATT
  AAACTTTAAATGCCATTACTACTTGTTTTTTCATATCCCAAGAATAAGAACATGTCTATG
  TACCCGTTTCTAGGAAGGAGGAGTATATGCCTTTTGTCTATTTCTGACACAGATGCATTT
  GGTCTGTCAAAACTGCAACCATGGGGTATGTAGTATGACCAACTGCATTTAACAAAACTC
  CTCTAAGTACTTCTAAATATTCTGTGCAAATATCTTGAGAACTTCAACATCCTAAAAATG
  TGTTTCTACAAGACTTTATATTTTAAGCTAAGTGGAATAATACAACGTAACTAAAACATG
  GCGGGTACCGAAAGGAGGGCAATTCATGGAAGAAATTTCCTCTGTTCATTCCGTTCTCTT
  CAACCTAAGCAATATGGATGTGTGAGTGTGTGATTTATTATGGTACCAAAAATGTCCACC
  TTCTTTCCTGCAGCGGAATAAGCTTTTTTGAAATGCTGAGGTTTTATTTTAATAGTTGAC
  AACCTGGGAAAGTTCTGAGTATTTATTTTACTGCTCTTTTTTGTGCATAGAAAGGTTTAG
  TAGGGTACTTTTTTGCATTAATTTGCCAATACTCTTATTCTTTGTTCTAAATAAAGTGAC
  TAGAATTTTTGTGTACTATTCAATTAAAGGAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015939 unedited
  AAAGGTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGAATCANATCTGGTACCGGT
  CCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGTGAGGCTGGCGGTGGCGACAACC
  GAGGAGGAGGGGCGGGACGCCGTGGAGCACGGCGACCGGCTGAGCGTCATGGAGGGCTCA
  GGGGAGCAGCCGGGCCCACAACCACAGCATCCCGGAGACCACCGCATCCGCGACGGCGAC
  TTCGTGGTGCTGAAACGTGAAGATGTGTTTAAAGCAGTACAAGTCCAGCGGAGAAAAAAA
  GTAACTTTCGAAAAACAGTGGTTCTACCTGGATAACGTCATTGGCCATAGTTATGGAACT
  GCATTTGAAGTGACCAGTGGAGGAAGTCTACAGCCCAAGAAGAAGAGGGAAGAGCCTACT
  GCAGAGACTAAAGAAGCGGGCACTGATAATCGAAATATAGTTGATGATGGGAAATCTCAG
  AAACTTACTCAAGATGACATAAAAGCTTTGAAGGACAAGGGCATTAAAGGAGAGGAAATA
  GTTCAGCAGTTAATTGAAAATAGTACAACATTCCGAGACAAGACAGAATTTGCCCAAGAT
  AAATATATTAAAAAGAAGAAAAAAAAATATGAAGCCATCATTACTGTTGTGAAGCCATCC
  ACCCGTATTCTTTCAATTATGTATTATGCAAGAGAACCTGGAAAAATAACCACATGAGAT
  ACGATACACTAGCCCAGATGTTGACGTTGGGAAATATCCGTGCTGGCAACAAAATGATTG
  TGATGGAAACGTGTGCANGCTTGGTGCTGGGGTGCATGATGGAACGAATGGGAGGTNTTT
  GGCTCCATTATCAGCTATACCCTGGAGGGAGACCTGTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015939
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015939.3, NP_057023.2
• RefSeq Size: 2311 bp
• RefSeq ORF: 1494 bp
• Locus ID: 51605
• UniProt ID: Q9UJA5
• Cytogenetics: 20p12.3
• Gene Summary: This gene encodes a member of the tRNA methyltransferase 6 protein family. A similar protein in yeast is part of a two component methyltransferase, which is involved in the posttranslational modification that produces the modified nucleoside 1-methyladenosine in tRNAs. Modified 1-methyladenosine influences initiator methionine stability and may be involved in the replication of human immunodeficiency virus type 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).