Pet1 (FEV) (NM_017521) Human Untagged Clone

SKU SC126133

FEV (untagged)-Human FEV (ETS oncogene family) (FEV)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Pet1
• Synonyms: HSRNAFEV; PET-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017521 edited; AGAGAGTCAGGGAGGCGGAAAAGACACGCGCTCTAGGCTCCCATCAGGGCACATGGCCCG; GGCCCATCCCCCGCGCGTCTCCCCGGCTGCGGGGCGCGGGGGGCTGCCGGGTGCGCTTGG; CTGTGGCGCGGCGCGTTGGAGACTTTATTGCGATGGGACGATAAGAGGGGCGGGGGCGGG; GTCCTGGGGGCCGAGGCGGCAGCGCTTTAATTAAAACGGAAATTGCGGCCCCGGGCCGCG; CGGGGGCCGGAGGGTTCCAAGCGGCCCCTTAGCTGGAAGCGTTTCTCCAGGACCCCCCCG; CAACCCCCGCCACGCCCGGGCTGCCCCCTCCCGCCAGGCCCTGCCGGACCCGGCGCCGTC; TTCTCCTCCTTGTCACCCGCGGTCGCTTCGGGCGGGGATCGGTGCCACCGAGCGCAAAGC; CTGCCTCGCCCCCCTTCCCCGTCCCCCCCATCTCCCACCGCCCAGTCCCCGGCGGCGATG; AGACAGAGCGGCGCCTCCCAGCCCCTGCTGATCAACATGTACCTGCCAGATCCCGTCGGA; GACGGTCTCTTCAAGGACGGGAAGAACCCGAGCTGGGGGCCGCTGAGCCCCGCGGTTCAG; AAAGGCAGCGGACAGATCCAGCTGTGGCAGTTTCTGCTGGAGCTGCTGGCTGACCGCGCG; AACGCCGGCTGCATCGCGTGGGAGGGCGGTCACGGCGAGTTCAAGCTCACGGACCCGGAC; GAGGTGGCGCGGCGGTGGGGCGAGCGCAAGAGCAAGCCCAACATGAACTACGACAAGCTG; AGCCGCGCCCTGCGCTACTACTACGACAAGAACATCATGAGCAAGGTGCATGGCAAGCGC; TACGCCTACCGCTTCGACTTCCAGGGCCTGGCGCAGGCCTGCCAGCCGCCGCCCGCGCAC; GCTCATGCCGCCGCCGCAGCTGCTGCCGCCGCCGCGGCCGCCCAGGACGGCGCGCTCTAC; AAGCTGCCCGCCGGCCTCGCCCCGCTGCCCTTCCCCGGCCTCTCCAAACTCAACCTCATG; GCCGCCTCGGCCGGGGTCGCGCCCGCCGGCTTCTCCTACTGGCCGGGCCCGGGCCCCGCC; GCCACCGCTGCCGCCGCCACCGCCGCGCTCTACCCCAGTCCCAGCTTGCAGCCCCCGCCC; GGGCCCTTCGGGGCCGTGGCCGCAGCCTCGCACTTGGGGGGCCATTACCACTAGACGGGG; CGGTCGGGTGCCTGCGGCCTCGCCCGCACGCCTAGAGTCTCGCCCGATCCCATCGGCATC; CCGGGGAGGGCCCGGGAGCCTCCGTCAACCGTCCTCTAATCCAGAGTTTACTCCACCTGC; CGCACTTAGCAGGGGGACGGGACCGAAGCTCCCTCAATCCTTGTCTGGTACTAGATTTGC; TCCTGTCCCACCCCGCAGTCCCCTGAGGAGGGCGATGTGCGCCCTCTTTCACTTTTTTTC; TTCTAGGTCTCCAGGTCCCGGAGGGGATTTGTGGACCTCTCTTGTCTCCCCACCACTCCA; GTGCATTTCCGCCTGGCTCCTAGAAGCCCCATTCAATATCACTACTCTTTAACGAGTGCC; AAATCTTTTCCCACTTTTGCTCTTCCCCAAGGAACTGCTCCCACCTCAGCACGTGGAGGC; CTCTCACGGTCCTCCTTCCTGGGACCTGAGCAGGTTTGGTGAAAGCCACCGTCCTCCGTG; ACACACGGCCCCCTTCCTCCTGTCCCCACACTCCCAGGAGAAACTCCCGGTGTGTTTCTG; ACCCTTTCAGCCCCATTAAAGCTCCTGAGCTCTCAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017521 unedited; CATATTTGTATACACTCACTATAGGCGGCCGCGTAATTCGCACGAGGAGAGATCAGGGAG; GCGGAAAAGACACGCGCTCTAGGCTCCCATCAGGGCACATGGCCCGGGCCCATCCCCCGC; GCGTCTCCCCGGCTGCGGGGCGCGGGGGGCTGCCGGGTGCGCTTGGCTGTGGCGCGGCGC; GTTGGAGACTTTATTGCGATGGGACGATAAGAGGGGCGGGGGCGGGGTCCTGGGGGCCGA; NGCNNNNNNNNTTTNNTTAANANNGANNTTGNGGCCNCGGGCCGCGCGGGGGCCGGNGGG; TTCCNGGCGGCCCCTTNNCTGGGNGCGTTTCTCTCGGCCCCCCCCGCNTCCCCCGCCCCG; CCCGGGCTGCCCCCTCCCGCCCGGCCCTGGCCGGCCCCGGCGCCTTCTTTTNCCTTCTTG; TTTCCCGGTGTTTCTTTCTGCCGGGGTCTGTGCCCGCCCTGCTCNGGTCCTTTCTGCCCC; CGGTTTTGCGTTTGCTCCTCGCGTCCCCTTCCTCTTTCCCGGCTGCCCNGTTCTCTTTTT; GCCCTGCNTCTTGTTCTTGCTGCTGTTCTGCTTTTTGTGTCGCTTCTTCTTNTCTGTGTG; TCTTTGTCCTTGCTCTCTGTCCTTTTCGCCCCCCCCCCTCCCGTCGCNTTGTTCCCCTCC; TGTTCCGGTTTGGTTCTGCTCTGCCTGCTGCCGTCCGGTTCTCTTCTTCCGCCGCGTGTT; GTTTTGGTGGCGCCCGGTGGGCGTATGGCCGGTCGGTTTCGGTCCCGTCTTTCTCTCCTC; CNGCGNTGTCTCTGTCCTTCTCCTTTNTTTTCCGCCTGTGCTGTCGTTGTGTCGTTGGCT; GCTTTCTGTGCTCGGTGTCTGTTTTTTTGTCTTCTTTCCGCCTTTCCTTCTTCTCTTCCG; CTCCTCCTTCTGCGTTGTGGTTGTGTGCGTGTTCCCTGTCTTTGTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_017521
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017521.2, NP_059991.1
• RefSeq Size: 1901 bp
• RefSeq ORF: 717 bp
• Locus ID: 54738
• UniProt ID: Q99581
• Cytogenetics: 2q35
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene belongs to the ETS transcription factor family. ETS family members have a highly conserved 85-amino acid ETS domain that binds purine-rich DNA sequences. The alanine-rich C-terminus of this gene indicates that it may act as a transcription repressor. This gene is exclusively expressed in neurons of the central serotonin (5-HT) system, a system implicated in the pathogeny of such psychiatric diseases as depression, anxiety, and eating disorders. In some types of Ewing tumors, this gene is fused to the Ewing sarcoma (EWS) gene following chromosome translocations. [provided by RefSeq, Jul 2008]