PRPS1L1 (NM_175886) Human Untagged Clone

SKU SC126124

PRPS1L1 (untagged)-Human phosphoribosyl pyrophosphate synthetase 1-like 1 (PRPS1L1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PRPS1L1
• Synonyms: PRPS1; PRPS3; PRPSL; PRS-III
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_175886 edited; GGGGAGCTGGATCGCTCGACGGAGTGCCTCTGGTAGTTGGCCAAGACGCCGAATATCAAA; ATCTTCAGCGGCAGCTCCCACCAGGACTTATCCCAGAAAATTGCTGACCGCCTGGGCCTG; GAGCTAGGCAAGGTGGTGACTAAGAAATTCAGCAACCAGGAGACCTGCGTGGAAATTGAT; GAGAGTGTGCGTGGAGAGGATGTCTACATCGTTCAGAGTGGTTGTGGCGAAATCAACGAC; AGTCTAATGGAGCTTTTGATCATGATTAATGCCTGCAAGATTGCTTCAGCTAGCCGAGTT; ACTGCAGTCATCCCATGCTTCCCTTATGCCCGACAGGATAAGAAGGATAAGAGCCGGTCC; CCAATCTCTGCCAAGCTTGTTGCAAATATGCTCTCTATAGCAGGTGCGGATCATATCATC; ACCATGGACCTACATGCTTCTCAAATTCAGGGCTTTTTTGATATCCCAGTAGACAACTTG; TATGCAGAGCCAACTGTCCTGAAGTGGATAAGGGAGAATATCCCTGAGTGGAAGAACTGC; ATTATTGTCTCGCCAGATGCTGGTGGAGCTAAAAGAGTGACCTCCATTGCAGACCAGTTG; AATGTGGACTTTGCTTTGATTCATAAAGAACGGAAGAAGGCCAATGAAGTGGACTGCATA; GTGCTAGTGGGAGATGTGAATGATCGTGTGGCTATCCTTGTAGATGACATGGCAGACACT; TGTGTTACAATCTGCCTCGCAGCTGACAAACTTCTCTCAGCTGGAGCAACCAGAGTTTAT; GCTATCTTGACTCATGGAATCTTTTCTGGCCCAGCCATTTCTCGCATCAACACTGCATGC; TTTGAAGCAGTGGTAGTCACCAATACCATACCTCAAGATGATAAGATGAAGCATTGCTCC; AAAATACGAGTAATTGACATCTCCATGATCCTTGCAGAAGCCATAAGGAGAACTCATAAT; GGGGAATCTGTTTCCTACCTGTTCAGCCATGTTCCTTTATAACAGAATAACTTCTAGGTT; ATGCTATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175886 unedited; TCGGTTCATATTTGTATACGACTCATATAGGCGGCCGCGTAATTCGCCATTACGGCCGGG; GAGCTGGATCGCTCGACGGATGCCTCTGGTAGTTGGCCAAGACGCCGAATATCAAAATCT; TCAGCGGCAGCTCCCACCAGGACTTATCCCAGAAAATTGCTGACCGCCTGGGCCTGGAGC; TAGGCAAGGTGGTGACTAAGAAATTCAGCAACCAGGAGACCTGCGTGGAAATTGATGAGA; GTGTGCGTGGAGAGGATGTCTACATCGTTCAGAGTGGTTGTGGCGAAATCAACGACAGTC; TAATGGAGCTTTTGATCATGATTAATGCCTGCAAGATTGCTTCAGCTAGCCGAGTTACTG; CAGTCATCCCATGCTTCCCTTATGCCCGACAGGATAAGAAGGATAAGAGCCGGTCCCCAA; TCTCTGCCAAGCTTGTTGCAAATATGCTCTCTATAGCAGGTGCGGATCATATCATCACCA; TGGACCTACATGCTTCTCAAATTCAGGGCTTTTTTGATATCCCAGTAGACAACTTGTATG; CAGAGCCAACTGTCCTGAAGTGGATAAGGGAGAATATCCCTGAGTGGAAGAACTGCATTA; TTGTCTCGCCAGATGCTGGTGGAGCTAANAGAGTGACCTCCATTGCAGACCAGTTGAATG; TGGACTTTGCTTTGATTCATAAAGAACGGAAGAAGGCCAATGAAGTGGACTGCATAGTGC; TAGTGGGAGATGTGAATGATCGTGTGGCTATCCTTGTAGATGACATGGCAGACACTTGTG; TTACAATCTGCCTCGCAGCTGAACAACTTCTCTCAGCTGGAGCAACCAGAGTTTATGCTA; TCTTGACTCATGGAATCTTTTCTGGCCCACCATTTCTCGCATCACACTGCATGCTTTGAA; GCAGTGGAANCAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_175886
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_175886.2, NP_787082.1
• RefSeq Size: 1091 bp
• RefSeq ORF: 957 bp
• Locus ID: 221823
• UniProt ID: P21108
• Cytogenetics: 7p21.1
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway, Purine metabolism
• Summary: This intronless gene is specifically expressed in the testis, and encodes a protein that is highly homologous to the two subunits of phosphoribosylpyrophosphate synthetase encoded by human X-linked genes, PRPS1 and PRPS2. These enzymes convert pyrimidine, purine or pyridine bases to the corresponding ribonucleoside monophosphates. In vitro transcription/translation and site-directed mutagenesis studies indicate that translation of this mRNA initiates exclusively at a non-AUG (ACG) codon. [provided by RefSeq, Jul 2008]