HYPE (FICD) (NM_007076) Human Untagged Clone

SKU SC125628

FICD (untagged)-Human FIC domain containing (FICD)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HYPE
• Synonyms: HIP13; HYPE; UNQ3041
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_007076 edited; GCTGGGAGCGCGGGGAAGGCGGTTGGGGTTCTGACAGCTGCGCGCGATCCTGCTCTCTCT; CAGCCGCCTGTGGACATGCGCAAAGGGCCCTCTCCTGAGTCCAGATGATGCTCATACCAA; TGGCTTCAGTGATGGCGGTGACTGAACCGAAATGGGTCTCGGTCTGGAGCCGCTTCCTCT; GGGTGACGCTGCTGAGCATGGTGCTGGGGTCCCTGCTGGCCCTGCTGCTGCCGCTGGGGG; CTGTGGAGGAGCAGTGCTTGGCTGTGCTCAAAGGCCTCTACCTGCTCAGGAGCAAACCGG; ACAGGGCGCAGCATGCCGCCACCAAGTGCACCAGCCCGTCCACGGAGCTCAGCATCACCT; CCAGGGGCGCGACGCTGCTGGTGGCCAAGACCAAGGCCTCTCCAGCGGGTAAGTTGGAAG; CCAGAGCTGCCCTGAACCAGGCCCTGGAGATGAAGCGCCAGGGCAAGCGGGAAAAAGCCC; AAAAGCTCTTCATGCACGCCCTCAAGATGGACCCGGACTTCGTGGACGCGCTCACCGAGT; TTGGCATCTTCTCGGAAGAAGACAAGGACATCATCCAGGCGGACTACTTGTACACCAGAG; CATTGACCATCTCACCCTACCATGAGAAAGCACTGGTCAACCGCGATCGGACACTGCCTC; TTGTGGAAGAGATCGACCAGAGGTATTTCAGCATCATCGACAGCAAAGTGAAGAAGGTCA; TGTCCATCCCCAAGGGGAACTCAGCTCTGCGCAGGGTCATGGAGGAGACCTACTACCATC; ACATCTACCACACAGTGGCCATCGAGGGCAACACCCTCACCCTCTCGGAAATCAGGCACA; TCCTGGAGACCCGCTACGCCGTGCCCGGGAAGAGCCTGGAGGAGCAGAACGAGGTCATAG; GCATGCATGCAGCCATGAAGTACATCAACACGACTCTGGTTTCGCGCATCGGCTCCGTCA; CCATCAGCGACGTGCTGGAGATCCACAGGCGGGTGCTGGGCTACGTGGACCCCGTGGAAG; CCGGCAGGTTTCGGACAACACAGGTCCTGGTCGGACACCACATCCCTCCCCATCCGCAGG; ATGTGGAAAAGCAGATGCAGGAGTTTGTACAGTGGCTCAACTCCGAGGAAGCCATGAACC; TGCACCCAGTGGAGTTTGCAGCCTTAGCCCATTATAAACTCGTTTACATCCACCCTTTCA; TTGATGGCAACGGGAGGACCTCCCGTCTGCTCATGAACCTCATCCTCATGCAGGCGGGCT; ACCCGCCCATCACCATCCGCAAGGAGCAGCGGTCCGACTACTACCACGTGTTGGAAGCTG; CCAACGAGGGCGACGTGAGGCCTTTCATTCGCTTCATCGCCAAGTGTACTGAGACCACCC; TGGACACCCTGCTTTTTGCCACAACTGAGTACTCGGTGGCACTGCCAGAAGCCCAACCCA; ACCACTCTGGGTTCAAGGAGACGCTTCCTGTGAAGCCCTAACCCTAGAAATCCTCAGTGA; CAAAGGCTGTCCTGAGGTAGGAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007076 unedited; GGATAGGATTTGTATACGACTTACTATAGGCGGACCGCGAATCAAATCTGGTACCGGTCC; GGAATTCCCGGGATATCGTCGACCCACGCGTCCGGCTGGGAGCGCGGGGAAGGCGGTTGG; GGTTCTGACAGCTGCGCGCGATCCTGCTCTCTCTCAGCCGCCTGTGGACATGCGCAAAGG; GCCCTCTCCTGAGTCCAGATGATGCTCATACCAATGGCTTCAGTGATGGCGGTGACTGAA; CCGAAATGGGTCTCGGTCTGGAGCCGCTTCCTCTGGGTGACGCTGCTGAGCATGGTGCTG; GGGTCCCTGCTGGCCCTGCTGCTGCCGCTGGGGGCTGTGGAGGAGCAGTGCTTGGCTGTG; CTCAAAGGCCTCTACCTGCTCAGGAGCAAACCGGACAGGGCGCAGCATGCCGCCACCAAG; TGCACCAGCCCGTCCACGGAGCTCAGCATCACCTCCAGGGGCGCGACGCTGCTGGTGGCC; AAGACCAAGGCCTCTCCAGCGGGTAAGTTGGAAGCCAGAGCTGCCCTGAACCAGGCCCTG; GAGATGAAGCGCCAGGGCAAGCGGGAAAAAGCCCAAAAGCTCTTCATGCACGCCCTCAAG; ATGGACCCGGACTTCGTGGACGCGCTCACCGAGTTTGGCATCTTCTCGGGAAGAGAACAG; GACATCATCCAGGCGGACTACTTGTACACCAGAGCATTGACCATCTCACCCTACCATGAG; AAAGCACTGGTCAACCCGCGATCGGACACTGGCTCTTGTGGGAGAAATCGACCAGAGGTA; TTTCAGCATCATCGACAGCAAAGTGAAGAAGGTCATGTTCATCCCCCAAGGGGAACTCAC; CTCTTGCCCAGGGCATTGGAGGAAACCTACTACCATGACATGCTCCACAACGTGGGCCAT; CGAGGGGCACACCCCTCCCCTCT;
• Restriction Sites: Please inquire
• ACCN: NM_007076
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007076.2, NP_009007.2
• RefSeq Size: 1651 bp
• RefSeq ORF: 1377 bp
• Locus ID: 11153
• UniProt ID: Q9BVA6
• Cytogenetics: 12q23.3
• Protein Families: Transmembrane
• Summary: Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity). The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity). Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity). In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity). Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083).[UniProtKB/Swiss-Prot Function]