CH25H (NM_003956) Human Untagged Clone

CAT#: SC125616

CH25H (untagged)-Human cholesterol 25-hydroxylase (CH25H)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CH25H
• Synonyms: C25H
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003956 edited
  AGCTGCCTTGCACAGCCTCGCAATGAGCTGCCACAACTGCTCCGACCCCCAGGTCCTTTG
  CAGCTCCGGGCAGCTGTTCCTGCAGCCCCTCTGGGACCACCTGAGGAGCTGGGAGGCCCT
  CCTACAGTCGCCCTTCTTCCCGGTCATCTTCTCCATCACCACATACGTGGGCTTTTGCCT
  GCCCTTCGTGGTCCTGGATATCCTGTGCTCCTGGGTGCCCGCCCTGCGGCGCTACAAGAT
  CCACCCTGACTTCTCGCCATCCGCGCAGCAGCTGCTACCTTGCCTGGGGCAGACCCTCTA
  CCAGCATGTGATGTTTGTGTTCCCCGTGACGCTGCTGCATTGGGCCCGCAGCCCGGCCCT
  CCTGCCCCACGAAGCTCCCGAGCTGCTCCTGCTGCTGCACCACATCCTGTTCTGCCTGCT
  ACTCTTCGACATGGAGTTCTTCGTGTGGCACCTGCTGCACCACAAGGTGCCCTGGCTGTA
  CCGCACCTTCCACAAGGTGCACCACCAGAACTCGTCCTCGTTCGCGCTGGCAACGCAGTA
  TATGAGCGTCTGGGAACTGTTTTCTTTGGGCTTCTTCGACATGATGAACGTCACACTGCT
  CGGGTGCCACCCGCTCACCACCCTGACCTTCCACGTGGTCAACATCTGGCTTTCCGTGGA
  GGACCACTCCGGCTACAACTTCCCTTGGTCCACTCACAGACTGGTGCCCTTCGGGTGGTA
  CGGGGGTGTGGTGCACCACGACCTGCATCACTCTCACTTTAACTGCAACTTCGCTCCGTA
  CTTTACACACTGGGACAAAATACTGGGAACGCTGCGGACTGCATCTGTCCCAGCGCGGTG
  ATGTGGCTGCGGTGGGTGCCCCTAAGACTCGGGACTGCTGTGCCTTTCACACTTGAATGA
  AGAGAAACACCTGAGCTATATATTTTTTTAAAGCAACTAACTTATTGCTTTATGTTTATC
  TATGAAAACCATAGATAAAATCTGATGCATTTTTGTAATCTGACAAAGTAATTTACATAC
  TGTTTGTGTATCAATACAATTTTGTGTTCTTGGTATTCTTAGTCTAGCTCACCTCAATAG
  CCTTGAATCCTGCATATGAATTAGACATTCATCACTGGCATATTTAGAATATCTCTAAAA
  GGACTTGTTTGTAGAATAAGGAATTTTCTATGTTTCAAAGTGTTCTAAAACCTGGCTAAA
  AGAATGTATTTTTGTGGATGGTGTTGACTTCTGACTCTAAAAGCAATCAAACATGTTTCT
  GCTGGACAGTGACCAAGAATTATAGTACCTTCTTATATTTTTTTATAGAACTGTATATTT
  ATTTTGAAAGAAATGTTATTCGTGCTTTAAAAAGGAAAAAAAACCATGAATCAAATAAAA
  GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003956 unedited
  GTTCAGCGATATTTGTNAATACGCACTGCACTATAGGGGCGGGCCGCGCAATTCCGCCAT
  TACGGCCGGGGAGCTGCCTTGCACAGCCTCGCAGGAGCTGCCACAACTGCTCCGACCCCC
  AGGTCCTTTGCAGCTCCGGGCAGCTGTTCCTGCAGCCCCTCTGGGACCACCTGAGGAGCT
  GGGAGGCCCTCCTACAGTCGCCCTTCTTCCCGGTCATCTTCTCCATCACCACATACGTGG
  GCTTTTGCCTGCGCGGCGTGGTCCTGGATATCCTGTGCTCCTGGGTGCCCGCCCTGCGGC
  GCTACAAGATCCACCCTGACTTCTCGCCATCCGCGCAGCAGCTGCTACCTTGCCTGGGGC
  AGACCCTCTACCAGCATGTGATGTTTGTGTTCCCCGTGACGCTGCTGCATTGGGCCCGCA
  GCCCGGCCCTCCTGCCCCACGAAGCTCCCGAGCTGCTCCTGCTGCTGCACCACATCCTGT
  TCTGCCTGCTACTCTTCGACATGGAGTTCTTCGTGTGGCACCTGCTGCACCACAAGGTGC
  CCTGGCTGTACCGCACCTTCCACAAGGTGCACCACCAGAACTCGTCCTCGTTCGCGCTGG
  CAACGCAGTATATGAGCGTCTGGGGACTGTTTTCTTTGGGCTTCTTCGACATGATGAACG
  TCACACTGGGTCGGGTGCGACCCGCTCACCACCCTGAGCTTCCACGTGGTCAACATCTGG
  GCTTTCCGCTGGAGGACCACTTCCGGCTACCAACTTCCCTTTGGGCCACTCACAGGACTG
  GTGGCCCCTTCGGTGGGGGACGGGGGGTGGTGGTGGCACCCACAACCCTGGGTCAACTCT
  CACTTTTAACTGGCAACTTTCGTTCCCGTACTTTTAACCACCTGGGGACCAAATAACTGG
  GGAAACCGTTCGGGAACTGGAATTCTGTTCCCCACACCCCGGTGAATGTTGGCTCTCCCG
  GGGGGTGGCCCCTCTAAAACATCTCGGGACTGGCGTGGCGCGCTTCCACACTTGAG
  
• Restriction Sites: Please inquire
• ACCN: NM_003956
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003956.2, NP_003947.1
• RefSeq Size: 1411 bp
• RefSeq ORF: 819 bp
• Locus ID: 9023
• UniProt ID: O95992
• Cytogenetics: 10q23.31
• Protein Families: Transmembrane
• Protein Pathways: Primary bile acid biosynthesis
• Gene Summary: This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]