CH25H (NM_003956) Human Untagged Clone

SKU SC125616

CH25H (untagged)-Human cholesterol 25-hydroxylase (CH25H)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CH25H
• Synonyms: C25H
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003956 edited; AGCTGCCTTGCACAGCCTCGCAATGAGCTGCCACAACTGCTCCGACCCCCAGGTCCTTTG; CAGCTCCGGGCAGCTGTTCCTGCAGCCCCTCTGGGACCACCTGAGGAGCTGGGAGGCCCT; CCTACAGTCGCCCTTCTTCCCGGTCATCTTCTCCATCACCACATACGTGGGCTTTTGCCT; GCCCTTCGTGGTCCTGGATATCCTGTGCTCCTGGGTGCCCGCCCTGCGGCGCTACAAGAT; CCACCCTGACTTCTCGCCATCCGCGCAGCAGCTGCTACCTTGCCTGGGGCAGACCCTCTA; CCAGCATGTGATGTTTGTGTTCCCCGTGACGCTGCTGCATTGGGCCCGCAGCCCGGCCCT; CCTGCCCCACGAAGCTCCCGAGCTGCTCCTGCTGCTGCACCACATCCTGTTCTGCCTGCT; ACTCTTCGACATGGAGTTCTTCGTGTGGCACCTGCTGCACCACAAGGTGCCCTGGCTGTA; CCGCACCTTCCACAAGGTGCACCACCAGAACTCGTCCTCGTTCGCGCTGGCAACGCAGTA; TATGAGCGTCTGGGAACTGTTTTCTTTGGGCTTCTTCGACATGATGAACGTCACACTGCT; CGGGTGCCACCCGCTCACCACCCTGACCTTCCACGTGGTCAACATCTGGCTTTCCGTGGA; GGACCACTCCGGCTACAACTTCCCTTGGTCCACTCACAGACTGGTGCCCTTCGGGTGGTA; CGGGGGTGTGGTGCACCACGACCTGCATCACTCTCACTTTAACTGCAACTTCGCTCCGTA; CTTTACACACTGGGACAAAATACTGGGAACGCTGCGGACTGCATCTGTCCCAGCGCGGTG; ATGTGGCTGCGGTGGGTGCCCCTAAGACTCGGGACTGCTGTGCCTTTCACACTTGAATGA; AGAGAAACACCTGAGCTATATATTTTTTTAAAGCAACTAACTTATTGCTTTATGTTTATC; TATGAAAACCATAGATAAAATCTGATGCATTTTTGTAATCTGACAAAGTAATTTACATAC; TGTTTGTGTATCAATACAATTTTGTGTTCTTGGTATTCTTAGTCTAGCTCACCTCAATAG; CCTTGAATCCTGCATATGAATTAGACATTCATCACTGGCATATTTAGAATATCTCTAAAA; GGACTTGTTTGTAGAATAAGGAATTTTCTATGTTTCAAAGTGTTCTAAAACCTGGCTAAA; AGAATGTATTTTTGTGGATGGTGTTGACTTCTGACTCTAAAAGCAATCAAACATGTTTCT; GCTGGACAGTGACCAAGAATTATAGTACCTTCTTATATTTTTTTATAGAACTGTATATTT; ATTTTGAAAGAAATGTTATTCGTGCTTTAAAAAGGAAAAAAAACCATGAATCAAATAAAA; GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003956 unedited; GTTCAGCGATATTTGTNAATACGCACTGCACTATAGGGGCGGGCCGCGCAATTCCGCCAT; TACGGCCGGGGAGCTGCCTTGCACAGCCTCGCAGGAGCTGCCACAACTGCTCCGACCCCC; AGGTCCTTTGCAGCTCCGGGCAGCTGTTCCTGCAGCCCCTCTGGGACCACCTGAGGAGCT; GGGAGGCCCTCCTACAGTCGCCCTTCTTCCCGGTCATCTTCTCCATCACCACATACGTGG; GCTTTTGCCTGCGCGGCGTGGTCCTGGATATCCTGTGCTCCTGGGTGCCCGCCCTGCGGC; GCTACAAGATCCACCCTGACTTCTCGCCATCCGCGCAGCAGCTGCTACCTTGCCTGGGGC; AGACCCTCTACCAGCATGTGATGTTTGTGTTCCCCGTGACGCTGCTGCATTGGGCCCGCA; GCCCGGCCCTCCTGCCCCACGAAGCTCCCGAGCTGCTCCTGCTGCTGCACCACATCCTGT; TCTGCCTGCTACTCTTCGACATGGAGTTCTTCGTGTGGCACCTGCTGCACCACAAGGTGC; CCTGGCTGTACCGCACCTTCCACAAGGTGCACCACCAGAACTCGTCCTCGTTCGCGCTGG; CAACGCAGTATATGAGCGTCTGGGGACTGTTTTCTTTGGGCTTCTTCGACATGATGAACG; TCACACTGGGTCGGGTGCGACCCGCTCACCACCCTGAGCTTCCACGTGGTCAACATCTGG; GCTTTCCGCTGGAGGACCACTTCCGGCTACCAACTTCCCTTTGGGCCACTCACAGGACTG; GTGGCCCCTTCGGTGGGGGACGGGGGGTGGTGGTGGCACCCACAACCCTGGGTCAACTCT; CACTTTTAACTGGCAACTTTCGTTCCCGTACTTTTAACCACCTGGGGACCAAATAACTGG; GGAAACCGTTCGGGAACTGGAATTCTGTTCCCCACACCCCGGTGAATGTTGGCTCTCCCG; GGGGGTGGCCCCTCTAAAACATCTCGGGACTGGCGTGGCGCGCTTCCACACTTGAG;
• Restriction Sites: Please inquire
• ACCN: NM_003956
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003956.2, NP_003947.1
• RefSeq Size: 1411 bp
• RefSeq ORF: 819 bp
• Locus ID: 9023
• UniProt ID: O95992
• Cytogenetics: 10q23.31
• Protein Families: Transmembrane
• Protein Pathways: Primary bile acid biosynthesis
• Summary: This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]