FLI1 (NM_002017) Human Untagged Clone

CAT#: SC125534

FLI1 (untagged)-Human Friend leukemia virus integration 1 (FLI1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FLI1
• Synonyms: BDPLT21; EWSR2; SIC-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125534 sequence for NM_002017 edited (data generated by NextGen Sequencing)
  ATGGACGGGACTATTAAGGAGGCTCTGTCGGTGGTGAGCGACGACCAGTCCCTCTTTGAC
  TCAGCGTACGGAGCGGCAGCCCATCTCCCCAAGGCCGACATGACTGCCTCGGGGAGTCCT
  GACTACGGGCAGCCCCACAAGATCAACCCCCTCCCACCACAGCAGGAGTGGATCAATCAG
  CCAGTGAGGGTCAACGTCAAGCGGGAGTATGACCACATGAATGGATCCAGGGAGTCTCCG
  GTGGACTGCAGCGTTAGCAAATGCAGCAAGCTGGTGGGCGGAGGCGAGTCCAACCCCATG
  AACTACAACAGCTATATGGACGAGAAGAATGGCCCCCCTCCTCCCAACATGACCACCAAC
  GAGAGGAGAGTCATCGTCCCCGCAGACCCCACACTGTGGACACAGGAGCATGTGAGGCAA
  TGGCTGGAGTGGGCCATAAAGGAGTACAGCTTGATGGAGATCGACACATCCTTTTTCCAG
  AACATGGATGGCAAGGAACTGTGTAAAATGAACAAGGAGGACTTCCTCCGCGCCACCACC
  CTCTACAACACGGAAGTGCTGTTGTCACACCTCAGTTACCTCAGGGAAAGTTCACTGCTG
  GCCTATAATACAACCTCCCACACCGACCAATCCTCACGATTGAGTGTCAAAGAAGACCCT
  TCTTATGACTCAGTCAGAAGAGGAGCTTGGGGCAATAACATGAATTCTGGCCTCAACAAA
  AGTCCTCCCCTTGGAGGGGCACAAACGATCAGTAAGAATACAGAGCAACGGCCCCAGCCA
  GATCCGTATCAGATCCTGGGCCCGACCAGCAGTCGCCTAGCCAACCCTGGAAGCGGGCAG
  ATCCAGCTGTGGCAATTCCTCCTGGAGCTGCTCTCCGACAGCGCCAACGCCAGCTGTATC
  ACCTGGGAGGGGACCAACGGGGAGTTCAAAATGACGGACCCCGATGAGGTGGCCAGGCGC
  TGGGGCGAGCGGAAAAGCAAGCCCAACATGAATTACGACAAGCTGAGCCGGGCCCTCCGT
  TATTACTATGATAAAAACATTATGACCAAAGTGCACGGCAAAAGATATGCTTACAAATTT
  GACTTCCACGGCATTGCCCAGGCTCTGCAGCCACATCCGACCGAGTCGTCCATGTACAAG
  TACCCTTCTGACATCTCCTACATGCCTTCCTACCATGCCCACCAGCAGAAGGTGAACTTT
  GTCCCTCCCCATCCATCCTCCATGCCTGTCACTTCCTCCAGCTTCTTTGGAGCCGCATCA
  CAATACTGGACCTCCCCCACGGGGGGAATCTACCCCAACCCCAACGTCCCCCGCCATCCT
  AACACCCACGTGCCTTCACACTTAGGCAGCTACTACTAG
  
  Clone variation with respect to NM_002017.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002017 unedited
  ACACGATTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAACAACAAACGTGCACAGG
  GGAGTGAGGGCAGGGCGCTCGCAGGGGGCACGCAGGGAGGGCCCAGGGCGCCAGGGAGGC
  CGCGCCGGGCTAATCCGAAGGGGCTGCGAGGTCAGGCTGTAACCGGGTCAATGTGTGGAA
  TATTGGGGGGCTCGGCTGCAGACTTGGCCAAATGGACGGGACTATTAAGGAGGCTCTGTC
  GGTGGTGAGCGACGACCAGTCCCTCTTTGACTCAGCGTACGGAGCGGCAGCCCATCTCCC
  CAAGGCCGACATGACTGCCTCGGGGAGTCCTGACTACGGGCAGCCCCACAAGATCAACCC
  CCTCCCACCACAGCAGGAGTGGATCAATCAGCCAGTGAGGGTCAACGTCAAGCGGGAGTA
  TGACCACATGAATGGATCCAGGGAGTCTCCGGTGGACTGCAGCGTTAGCAAATGCAGCAA
  GCTGGTGGGCGGAGGCGAGTCCAACCCCATGAACTACAACAGCTATATGGACGAGAAGAA
  TGGCCCCCCTCCTCCCAACATGACCACCAACGAGAGGAGAGTCATCGTCCCCGCAGACCC
  CACACTGTGGACACAGGAGCATGTGAGGCAATGGCTGGAGTGGGCCATAAAGGAGTACAG
  CTTGATGGAGATCGACACATCCTTTTTCCAGAACATGGATGGCANGGAACTGTGTAAAAT
  GAACAGGGATGACTTNNCTCGNGCACCACCCTCTACACACGGAAGTGCTGNTGTCACACC
  TCAGTTACCTCAGGNAATGNTCACTGCTGGNCCTATATACACCTCCACACCGACCATCCT
  NACGATGAGTGTCAAAGAGACCCTTCTATGACTCATCAGAAGAGAGCCTGGNGGCATACA
  TGAATTCTGGCCTCACAAAGTCCTCCCCTTGGAGGGNCACAACGATCAGTAGGATACAGA
  GCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002017
• Insert Size: 2800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002017.2, NP_002008.2
• RefSeq Size: 2957 bp
• RefSeq ORF: 1359 bp
• Locus ID: 2313
• UniProt ID: Q01543
• Cytogenetics: 11q24.3
• Domains: ETS, SAM_PNT
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.