Silencer of Death Domain (BAG4) (NM_004874) Human Untagged Clone

CAT#: SC125513

BAG4 (untagged)-Human BCL2-associated athanogene 4 (BAG4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Silencer of Death Domain
• Synonyms: BAG-4; SODD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125513 sequence for NM_004874 edited (data generated by NextGen Sequencing)
  ATGTCGGCCCTGAGGCGCTCGGGCTACGGCCCCAGTGACGGTCCGTCCTACGGCCGCTAC
  TACGGGCCTGGGGGTGGAGATGTGCCGGTACACCCACCTCCACCCTTATATCCTCTTCGC
  CCTGAACCTCCCCAGCCTCCCATTTCCTGGCGGGTGCGCGGGGGCGGCCCGGCGGAGACC
  ACCTGGCTGGGAGAAGGCGGAGGAGGCGATGGCTACTATCCCTCGGGAGGCGCCTGGCCA
  GAGCCTGGTCGAGCCGGAGGAAGCCACCAGGAGCAGCCACCATATCCTAGCTACAATTCT
  AACTATTGGAATTCTACTGCGAGATCTAGGGCTCCTTACCCAAGTACATATCCTGTAAGA
  CCAGAATTGCAAGGCCAGAGTTTGAATTCTTATACAAATGGAGCGTATGGTCCAACATAC
  CCCCCAGGCCCTGGGGCAAATACTGCCTCATACTCAGGGGCTTATTATGCACCTGGTTAT
  ACTCAGACCAGTTACTCCACAGAAGTTCCAAGTACTTACCGTTCATCTGGCAACAGCCCA
  ACTCCAGTCTCTCGTTGGATCTATCCCCAGCAGGACTGTCAGACTGAAGCACCCCCTCTT
  AGGGGGCAGGTTCCAGGATATCCGCCTTCACAGAACCCTGGAATGACCCTGCCCCATTAT
  CCTTATGGAGATGGTAATCGTAGTGTTCCACAATCAGGACCGACTGTACGACCACAAGAA
  GATGCGTGGGCTTCTCCTGGTGCTTATGGAATGGGTGGCCGTTATCCCTGGCCTTCATCA
  GCGCCCTCAGCACCACCCGGCAATCTCTACATGACTGAAAGTACTTCACCATGGCCTAGC
  AGTGGCTCTCCCCAGTCACCCCCTTCACCCCCAGTCCAGCAGCCCAAGGATTCTTCATAC
  CCCTATAGCCAATCAGATCAAAGCATGAACCGGCACAACTTTCCTTGCAGTGTCCATCAG
  TACGAATCCTCGGGGACAGTGAACAATGATGATTCAGATCTTTTGGATTCCCAAGTCCAG
  TATAGTGCTGAGCCTCAGCTGTATGGTAATGCCACCAGTGACCATCCCAACAATCAAGAT
  CAAAGTAGCAGTCTTCCTGAAGAATGTGTACCTTCAGATGAAAGTACTCCTCCGAGTATT
  AAAAAAATCATACATGTGCTGGAGAAGGTCCAGTATCTTGAACAAGAAGTAGAAGAATTT
  GTAGGAAAAAAGACAGACAAAGCATACTGGCTTCTGGAAGAAATGCTAACCAAGGAACTT
  TTGGAACTGGATTCAGTTGAAACTGGGGGCCAGGACTCTGTACGGCAGGCCAGAAAAGAG
  GCTGTTTGTAAGATTCAGGCCATACTGGAAAAATTAGAAAAAAAAGGATTATGA
  
  Clone variation with respect to NM_004874.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004874 unedited
  GCGCCCCAACCCCCCCCNCACCCGCNCGGTTCAAATTTGTATACGACTCATATAGGCGGC
  CGCGNAATTCGCACCAGGATCCCTGTCGGCCCTGAGGCGCTCGGGCTACGGCCCCAGTGA
  CGGTCCGTCCTACGGCCGCTACTACGGGCCTGGGGGTGGAGATGTGCCGGTACACCCACC
  TCCACCCTTATATCCTCTTCGCCCTGAACCTCCCCAGCCTCCCATTTCCTGGCGGGTGCG
  CGGGGGCGGCCCGGCGGAGACCACCTGGCTGGGAGAAGGCGGAGGAGGCGATGGCTACTA
  TCCCTCGGGAGGCGCCTGGCCAGAGCCTGGTCGAGCCGGAGGAAGCCACCAGGAGCAGCC
  ACCATATCCTAGCTACAATTCTAACTATTGGAATTCTACTGCGAGATCTAGGGCTCCTTA
  CCCAAGTACATATCCTGTAAGACCAGAATTGCAAGGCCAGAGTTTGAATTCTTATACAAA
  TGGAGCGTATGGTCCAACATACCCCCCAGGCCCTGNGGCAAATACTGCCTCATACTCAGG
  GGCTTATTATGCACCTGGTTATACTCAGACCAGTTACTCCACAGAAGTTCCAAGTACTTA
  CCGTTCATCTGGCAACAGCCCAACTCCAGTCTCTCGTTGGATCTATCCCCAGCAGGACTG
  TCAGACTGAAGCACCCCCTCTTAGGGGGCANNGTTCAGGATATCCGCCTTCACAGAACCC
  TGGAATGACCCTGCCCCATATCCCTATGGAGATGGTAATCGTAGTGGTTCACAATCCAGA
  CCGACTGTTCGACCCCAAGAAGAGCCTGGGCTTCTCCTGGTGCTAATGGAAGGGGGCCCG
  TATCCCTGGCCTTTATAAGGCCCTCAGCACCACCCGGAATCTTTACATGACTGAAAGTAC
  TTCCCATGGGCCTACAAGGGNTCTTTCCCAATCCCCCCTTAACCCCAATCCAGAGGCCCA
  GGATCTTTCTTCCCTTTTGGCCATTATATCAAGCTTGACCCGGCCAACTTTCCTGGAGGG
  GTCATAAAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004874
• Insert Size: 5050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004874.2, NP_004865.1
• RefSeq Size: 2182 bp
• RefSeq ORF: 1374 bp
• Locus ID: 9530
• UniProt ID: O95429
• Cytogenetics: 8p11.23
• Domains: BAG
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.