TIE1 (NM_005424) Human Untagged Clone

CAT#: SC125505

TIE1 (untagged)-Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TIE1
• Synonyms: JTK14; LMPHM11; TIE
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005424 edited
  CTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATT
  CGGCACGAGGAGCATCTGACCCCAGGCCCAGCTCGTCCTGGCTGGCCTGGGTCGGCCTCT
  GGAGTATGGTCTGGCGGGTGCCCCCTTTCTTGCTCCCCATCCTCTTCTTGGCTTCTCATG
  TGGGCGCGGCGGTGGACCTGACGCTGCTGGCCAACCTGCGGCTCACGGACCCCCAGCGCT
  TCTTCCTGACTTGCGTGTCTGGGGAGGCCGGGGCGGGGAGGGGCTCGGACGCCTGGGGCC
  CGCCCCTGCTGCTGGAGAAGGACGACCGTATCGTGCGCACCCCGCCCGGGCCACCCCTGC
  GCCTGGCGCGCAACGGTTCGCACCAGGTCACGCTTCGCGGCTTCTCCAAGCCCTCGGACC
  TCGTGGGCGTCTTCTCCTGCGTGGGCGGTGCTGGGGCGCGGCGCACGCGCGTCATCTACG
  TGCACAACAGCCCTGGAGCCCACCTGCTTCCAGACAAGGTCACACACACTGTGAACAAAG
  GTGACACCGCTGTACTTTCTGCACGTGTGCACAAGGAGAAGCAGACAGACGTGATCTGGA
  AGAGCAACGGATCCTACTTCTACACCCTGGACTGGCATGAAGCCCAGGATGGGCGGTTCC
  TGCTGCAGCTCCCAAATGTGCAGCCACCATCGAGCGGCATCTACAGTGCCACTTACCTGG
  AAGCCAGCCCCCTGGGCAGCGCCTTCTTTCGGCTCATCGTGCGGGGTTGTGGGGCTGGGC
  GCTGGGGGCCAGGCTGTACCAAGGAGTGCCCAGGTTGCCTACATGGAGGTGTCTGCCACG
  ACCATGACGGCGAATGTGTATGCCCCCCTGGCTTCACTGGCACCCGCTGTGAACAGGCCT
  GCAGAGAGGGCCGTTTTGGGCAGAGCTGCCAGGAGCAGTGCCCAGGCATATCAGGCTGCC
  GGGGCCTCACCTTCTGCCTCCCAGACCCCTATGGCTGCTCTTGTGGATCTGGCTGGAGAG
  GAAGCCAGTGCCAAGAAGCTTGTGCCCCTGGTCATTTTGGGGCTGATTGCCGACTCCAGT
  GCCAGTGTCAGAATGGTGGCACTTGTGACCGGTTCAGTGGTTGTGTCTGCCCCTCTGGGT
  GGCATGGAGTGCACTGTGAGAAGTCAGACCGGATCCCCCAGATCCTCAACATGGCCTCAG
  AACTGGAGTTCAACTTAGAGACGATGCCCCGGATCAACTGTGCAGCTGCAGGGAACCCCT
  TCCCCGTGCGGGGCAGCATAGAGCTACGCAAGCCAGACGGCACTGTGCTCCTGTCCACCA
  AGGCCATTGTGGAGCCAGAGAAGACCACAGCTGAGTTCGAGGTGCCCCGCTTGGTTCTTG
  CGGACAGTGGGTTCTGGGAGTGCCGTGTGTCCACATCTGGCGGCCAAGACAGCCGGCGCT
  TCAAGGTCAATGTGAAAGTGCCCCCCGTGCCCCTGGCTGCACCTCGGCTCCTGACCAAGC
  AGAGCCGCCAGCTTGTGGTCTCCCCGCTGGTCTCGTTCTCTGGGGATGGACCCATCTCCA
  CTGTCCGCCTGCACTACCGGCCCCAGGACAGTACCATGGACTGGTCGACCATTGTGGTGG
  ACCCCAGTGAGAACGTGACGTTAATGAACCTGAGGCCAAAGACAGGATACAGTGTTCGTG
  TGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAGGGGGCCTGGGGGCCTCCCACCCTCATGA
  CCACAGACTGTCCTGAGCCTTTGTTGCAGCCGTGGTTGGAGGGCTGGCATGTGGAAGGCA
  CTGACCGGCTGCGAGTGAGCTGGTCCTTGCCCTTGGTGCCCGGGCCACTGGTGGGCGACG
  GTTTCCTGCTGCGCCTGTGGGACGGGACACGGGGGCAGGAGCGGCGGGAGAACGTCTCAT
  CCCCCCAGGCCCGCACTGCCCTCCTGACGGGACTCACGCCTGGCACCCACTACCAGCTGG
  ATGTGCAGCTCTACCACTGCACCCTCCTGGGCCCGGCCTCGCCCCCTGCACACGTGCTTC
  TGCCCCCCAGTGGGCCTCCAGCCCCCCGACACCTCCACGCCCAGGCCCTCTCAGACTCCG
  AGATCCAGCTGACATGGAAGCACCCGGAGGCTCTGCCTGGGCCAATATCCAAGTACGTTG
  TGGAGGTGCAGGTGGCTGGGGGTGCAGGAGACCCACTGTGGATAGACGTGGACAGGCCTG
  AGGAGACAAGCACCATCATCCGTGGCCTCAACGCCAGCACGCGCTACCTCTTCCGCATGC
  GGGCCAGCATTCAGGGGCTCGGGGACTGGAGCAACACAGTAGAAGAGTCCACCCTGGGCA
  ACGGGCTGCAGGCTGAGGGCCCAGTCCAAGAGAGCCGGGCAGCTGAAGAGGGCCTGGATC
  AGCAGCTGATCCTGGCGGTGGTGGGCTCCGTGTCTGCCACCTGCCTCACCATCCTGGCCG
  CCCTTTTAACCCTGGTGTGCATCCGCAGAAGCTGCCTGCATCGGAGACGCACCTTCACCT
  ACCAGTCAGGCTCGGGCGAGGAGACCATCCTGCAGTTCAGCTCAGGGACCTTGACACTTA
  CCCGGCGGCCAAAACTGCAGCCCGAGCCCCTGAGCTACCCAGTGCTAGAGTGGGAGGACA
  TCACCTTTGAGGACCTCATCGGGGAGGGGAACTTCGGCCAGGTCATCCGGGCCATGATCA
  AGAAGGACGGGCTGAAGATGAACGCAGCCATCAAAATGCTGAAAGAGTATGCCTCTGAAA
  ATGACCATCGTGACTTTGCGGGAGAACTGGAAGTTCTGTGCAAATTGGGGCATCACCCCA
  ACATCATCAACCTCCTGGGGGCCTGTAAGAACCGAGGTTACTTGTATATCGCTATTGAAT
  ATGCCCCCTACGGGAACCTGCTAGATTTTCTGCGGAAAAGCCGGGTCCTAGAGACTGACC
  CAGCTTTTGCTCGAGAGCATGGGACAGCCTCTACCCTTAGCTCCCGGCAGCTGCTGCGTT
  TCGCCAGTGATGCGGCCAATGGCATGCAGTACCTGAGTGAGAAGCAGTTCATCCACAGGG
  ACCTGGCTGCCCGGAATGTGCTGGTCGGAGAGAACCTAGCCTCCAAGATTGCAGACTTCG
  GCCTTTCTCGGGGAGAGGAGGTTTATGTGAAGAAGACGATGGGGCGTCTCCCTGTGCGCT
  GGATGGCCATTGAGTCCCTGAACTACAGTGTCTATACCACCAAGAGTGATGTCTGGTCCT
  TTGGAGTCCTTCTTTGGGAGATAGTGAGCCTTGGAGGTACACCCTACTGTGGCATGACCT
  GTGCCGAGCTCTATGAAAAGCTGCCCCAGGGCTACCGCATGGAGCAGCCTCGAAACTGTG
  ACGATGAAGTGTACGAGCTGATGCGTCAGTGCTGGCGGGACCGTCCCTATGAGCGACCCC
  CCTTTGCCCAGATTGCGCTACAGCTAGGCCGCATGCTGGAAGCCAGGAAGGCCTATGTGA
  ACATGTCGCTGTTTGAGAACTTCACTTACGCGGGCATTGATGCCACAGCTGAGGAGGCCT
  GAGCTGCCATCCAGCCAGAACGTGGCTCTGCTGGCCGGAGCAAACTCTGCTGTCTAACCT
  GTGACCAGTCTGACCCTTACAGCCTCTGACTTAAGCTGCCTCAAGGAATTTTTTTAACTT
  AAGGGAGAAAAAAAGGGATCTGGGGATGGGGTGGGCTTAGGGGAACTGGGTTCCCATGCT
  TTGTAGGTGTCTCATAGCTATCCTGGGCATCCTTCTTTCTAGTTCAGCTGCCCCACAGGT
  GTGTTTCCCATCCCACTGCTCCCCCAACACAAACCCCCACTCCAGCTCCTTCGCTTAAGC
  CAGCACTCACACCACTAACATGCCCTGTTCAGCTACTCCCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005424 unedited
  GAAATTACGCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCATATCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGAGCATCTGACCCCAGGCCCAGCTCGTCCTGGCTGGCCTGGGTCG
  GCCTCTGGAGTATGGTCTGGCGGGTGCCCCCTTTCTTGCTCCCCATCCTCTTCTTGGCTT
  CTCATGTGGGCGCGGCGGTGGACCTGACGCTGCTGGCCAACCTGCGGCTCACGGACCCCC
  AGCGCTTCTTCCTGACTTGCGTGTCTGGGGAGGCCGGGGCGGGGAGGGGCTCGGACGCCT
  GGGGCCCGCCCCTGCTGCTGGAGAAGGACGACCGTATCGTGCGCACCCCGCCCGGGCCAC
  CCCTGCGCCTGGCGCGCAACGGTTCGCACCAGGTCACGCTTCGCGGCTTCTCCAAGCCCT
  CGGACCTCGTGGGCGTCTTCTCCTGCGTGGGCGGTGCTGGGGCGCGGCGCACGCGCGTCA
  TCTACGTGCACAACAGCCCTGGAGCCCACCTGCTTCCAGACAAGGTCACACACACTGTGA
  ACAAAGGTGACACCGCTGTACTTTCTGCACGTGTGCACAAGGAGAAGCAGACAGACGTGA
  TCTGGAAGAGCAACGGATCCTACTTCTACACCCTGGACTGGCATGAAGCCCAGGATGGGG
  CGGTTCCTGCTGCAGCTCCCAAATGTGCAGCCACCATCGAGCGGCATCTACAGTGCCACT
  TACCTGGAAGCCAGCCCCCTGGGCAGCGCCTTCTTTCGGCTCATCGTGCGGGGNTTGTGG
  GGCTTGGCGCTGGNGGCCCAGCTGTACCAAGGATGCCCCAGGTGCCTACTGGNAGGTGTC
  TGCCACGACATGACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005424 unedited
  AATGGGGGGNACATTCATTNATTCTGATGACAGGCCGGGCGTGGGAGTAGCTGAACAGGG
  CATGTTAGTGGTGTGAGTGCTGGCTTAAGCGAAGGAGCTGGAGTGGGGGTTTGTGTTGGG
  GGAGCAGTGGGATGGGAAACACACCTGTGGGGCAGCTGAACTAGAAAGAAGGATGCCCAG
  GATAGCTATGAGACACCTACAAAGCATGGGAACTCATTTTCCCTAAGCCCACCCCATCCC
  CAGATCCCTTTTTTTCTCCCTTAAGTTAAAAAAATTCCTTGAGGCAGCTTAAGTCAGAGG
  CTGTAAGGGTCAGACTGGTCACAGGTTAGACAGCAGAGTTTGCTCCGGCCAGCAGAGCCA
  CGTTCTGGCTGGATGGCAGCTCAGGCCTCCTCAGCTGTGGCATCAATGCCCGCGTAAGTG
  AAGTTCTCAAACAGCGACATGTTCACATAGGCCTTCCTGGCTTCCAGCATGCGGCCTAGC
  TGTAGCGCAATCTGGGCAAAGGGGGGTCGCTCATAGGGACGGTCCCGCCAGCACTGACGC
  ATCAGCTCGTACACTTCATCGTCACAGTTTCGAGGCTGCTCCATGCGGTAGCCCTGGGGC
  AGCTTTTCATAGAGCTCGGCACAGGTCATGCCACAGTAGGGTGTACCTCCAAGGCTCACT
  ATCTCCCAAAGAAGGACTTCCAAAGGACCAGACATCACTCTTTGTGGGTATAGACACTGT
  AGTTCAGGGACTCAATGGCCATCCAGCGCACAGGGAGACGCCCCATCGTCTTTTTCACAT
  AAACCTCCTCTCCCCGAGAAAGCCGGAATCTTGAATCTTGGAAGCTAGGTTCTTTCCGAC
  CAGACTTTCCGGCAGGCAGGTCCCTGTGGGAGAACTGGTTCTAACTCAGGACCTGCTGCA
  CTTGCCCGCTTCCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005424
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005424.2, NP_005415.1
• RefSeq Size: 4000 bp
• RefSeq ORF: 3417 bp
• Locus ID: 7075
• UniProt ID: P35590
• Cytogenetics: 1p34.2
• Domains: pkinase, TyrKc, S_TKc, ig, IG, FN3, EGF, EGF
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Gene Summary: This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).