DUSP4 (NM_057158) Human Untagged Clone

CAT#: SC125374

DUSP4 (untagged)-Human dual specificity phosphatase 4 (DUSP4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUSP4
• Synonyms: HVH2; MKP-2; MKP2; TYP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125374 sequence for NM_057158 edited (data generated by NextGen Sequencing)
  ATGGGAAGAAAAGTTCACTCCAACGGAAGCCAGTTTGCTGAACATAGCAGATCGCCCAGG
  AGGACTGGGAGAGACTGCAAACCAGTTCGAGCCCCCAGCATGGCGTTAGGTGTCAGCCAG
  CTGGCAGGAAGGTCCAGGTGTCTGTGTTCAGAGTCTCAAGGCGGCTATGAGAGGTTTTCC
  TCCGAGTACCCAGAATTCTGTTCTAAAACCAAGGCCCTGGCAGCCATCCCACCCCCGGTT
  CCCCCCAGTGCCACAGAGCCCTTGGACCTGGGCTGCAGCTCCTGTGGGACCCCACTACAC
  GACCAGGGGGGTCCTGTGGAGATCCTTCCCTTCCTCTACCTCGGCAGTGCCTACCATGCT
  GCCCGGAGAGACATGCTGGACGCCCTGGGCATCACGGCTCTGTTGAATGTCTCCTCGGAC
  TGCCCAAACCACTTTGAAGGACACTATCAGTACAAGTGCATCCCAGTGGAAGATAACCAC
  AAGGCCGACATCAGCTCCTGGTTCATGGAAGCCATAGAGTACATCGATGCCGTGAAGGAC
  TGCCGTGGGCGCGTGCTGGTGCACTGCCAGGCGGGCATCTCGCGGTCGGCCACCATCTGC
  CTGGCCTACCTGATGATGAAGAAACGGGTGAGGCTGGAGGAGGCCTTCGAGTTCGTTAAG
  CAGCGCCGCAGCATCATCTCGCCCAACTTCAGCTTCATGGGGCAGCTGCTGCAGTTCGAG
  TCCCAGGTGCTGGCCACGTCCTGTGCTGCGGAGGCTGCTAGCCCCTCGGGACCCCTGCGG
  GAGCGGGGCAAGACCCCCGCCACCCCCACCTCGCAGTTCGTCTTCAGCTTTCCGGTCTCC
  GTGGGCGTGCACTCGGCCCCCAGCAGCCTGCCCTACCTGCACAGCCCCATCACCACCTCT
  CCCAGCTGTTAG
  
  Clone variation with respect to NM_057158.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_057158 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGCCCTTCTAACGAAGAGGAAGCACGATG
  GGAAGAAAAGTTCACTCCAACGGAAGCCAGTTTGCTGAACATAGCAGATCGCCCAGGAGG
  ACTGGGAGAGACTGCAAACCAGTTCGAGCCCCCAGCATGGCGTTAGGTGTCAGCCAGCTG
  GCAGGAAGGTCCAGGTGTCTGTGTTCAGAGTCTCAAGGCGGCTATGAGAGGTTTTCCTCC
  GAGTACCCAGAATTCTGTTCTAAAACCAAGGCCCTGGCAGCCATCCCACCCCCGGTTCCC
  CCCAGTGCCACAGAGCCCTTGGACCTGGGCTGCAGCTCCTGTGGGACCCCACTACACGAC
  CAGGGGGGTCCTGTGGAGATCCTTCCCTTCCTCTACCTCGGCAGTGCCTACCATGCTGCC
  CGGAGAGACATGCTGGACGCCCTGGGCATCACGGCTCTGTTGAATGTCTCCTCGGACTGC
  CCAAACCACTTTGAAGGACACTATCAATACAAGTGCATNCCAGTTGAAAGATAACCACAN
  GGCCGACTTCAACTCCTGTTCATTGGANGCAATANAGTACATCAATGCCCTNAAGGACTG
  GCCTGGCCNCTTCCTGGTTCCATTGCCCAGCCGGCTATCTCGGGGTCGGCACCTTCTGCC
  TGCCCTAACTGGTGATGAAAAACCGGTGAGCTTGGAGAAGCCTTCGAGTTGTTAACAAAC
  CCGAACATTTTTTTGGCCAATTTAGTTTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_057158 unedited
  ACTTCAGGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTC
  AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTTGATATCGGTACCAGA
  TCTGAATTCGCCCTTCTGCTCAGTCCCAACTTTCTGCCCGCATGCGGCCCGTCCTACCCT
  TGCTGGGAGCCAGCTCTGGTTCTGGGGCCCCCAGGGCGGCTCTAACAGCTGGGAGAGGTG
  GTGATGGGGCTGTGCAGGTAGGGCAGGCTGCTGGGGGCCGAGTGCACGCCCACGGAGACC
  GGAAAGCTGAAGACGAACTGCGAGGTGGGGGTGGCGGGGGTCTTGCCCCGCTCCCGCAGG
  GGTCCCGAGGGGCTAGCAGCCTCCGCAGCACAGGACGTGGCCAGCACCTGGGACTCGAAC
  TGCAGCAGCTGCCCCATGAAGCTGAAGTTGGGCGAGATGATGCTGCGGCGCTGCTTAACG
  AACTCGAAGGCCTNCTCCAGCCTCACCGGTTCTTCATCATCAAGTAGGCCCAGCANATGG
  TGGCCCGACGCCAAGATGCCCGCCTGGCAATTGCACAACACGCCCCCCACGGAGTTCCTT
  CACGGCATTCAGGTACTCTATGCTTCCATGAACCAGAACTGATGTCGGCCTTGGGTTATC
  TCCCTGAAAGCCATTGTGCCGGTAGTGCCTCAAAGGGTTGGGCATCCGAGGAACTTAACA
  AACCGTGAGCCAAGGCTC
  
• Restriction Sites: Please inquire
• ACCN: NM_057158
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057158.2, NP_476499.1
• RefSeq Size: 3404 bp
• RefSeq ORF: 912 bp
• Locus ID: 1846
• UniProt ID: Q13115
• Cytogenetics: 8p12
• Protein Families: Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.