Oct4 (POU5F1) (NM_002701) Human Untagged Clone

CAT#: SC125252

POU5F1/OCT4 (untagged)-Human POU class 5 homeobox 1 (POU5F1/OCT4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Oct4
• Synonyms: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002701 edited
  CTGCGGGGTGGCTGGATTTGGCCAGTATCGGGATGGGAATGCCTAGGATTCTGGATGGAT
  CGGGGGAAGGCATAAGGGAGCAGCTGGCCATTGTGCTTATGGCTGTTGATGCATTGAGGG
  ATAGCGCCACACACACATTCAATAAATTTGAGGAGCTGAGAGGGTGACTGGCCCCTGAAG
  GCACAGTGCCAGAGGTCTGTGGAGAGGGGGTCAAGCACCTGGGTTCCTGAAGAACATGGA
  GGTGTGGGAGTGATTCCAGACAGCTGGGATGTGCAGAGCCTGAGAGAGTGCCAGGGAGCG
  GGTTGGGAGTTGAAAGTTGGGTGTGGTGGCTCACGCCTTTAATCATGACACTGGGCGGCA
  GAGGCGGGAGGATTTCTTGAGGACAGGAATTCAAGACCAGCCTGGGTAACATAGCAAGGC
  CCCATCTCTACTAAAAATAAAAAAACTAACAGGGCACAGTGGTCCAAGCCTGTAGTCCCA
  GCCACTTAGGAGGCTGGAGCAGAAGGATTGCTTTGGCCCAGTAGATCGAGGCTACATTGA
  GCCATCATTGTACTCCACTGCACTCCAGTCTGGGCAACAAAGTGAGACCCTGTCTTAAAA
  AATAAAAATAAAAAAAGTTTCTGTGGGGGACCTGCACTGAGGTCCTGGAGGGGCGCCAGT
  TGTGTCTCCCGGTTTTCCCCTTCCACAGACACCATTGCCACCACCATTAGGCAAACATCC
  TTCGCCTCAGTTTCTCCCCCCACCTCCCTCTCCTCCACCCATCCAGGGGGCGGGGCCAGA
  GGTCAAGGCTAGTGGGTGGGACTGGGGAGGGAGAGAGGGGTTGAGTAGTCCCTTCGCAAG
  CCCTCATTTCACCAGGCCCCCGGCTTGGGGCGCCTTCCTTCCCCATGGCGGGACACCTGG
  CTTCGGATTTCGCCTTCTCGCCCCCTCCAGGTGGTGGAGGTGATGGGCCAGGGGGGCCGG
  AGCCGGGCTGGGTTGATCCTCGGACCTGGCTAAGCTTCCAAGGCCCTCCTGGAGGGCCAG
  GAATCGGGCCGGGGGTTGGGCCAGGCTCTGAGGTGTGGGGGATTCCCCCATGCCCCCCGC
  CGTATGAGTTCTGTGGGGGGATGGCGTACTGTGGGCCCCAGGTTGGAGTGGGGCTAGTGC
  CCCAAGGCGGCTTGGAGACCTCTCAGCCTGAGGGCGAAGCAGGAGTCGGGGTGGAGAGCA
  ACTCCGATGGGGCCTCCCCGGAGCCCTGCACCGTCACCCCTGGTGCCGTGAAGCTGGAGA
  AGGAGAAGCTGGAGCAAAACCCGGAGGAGTCCCAGGACATCAAAGCTCTGCAGAAAGAAC
  TCGAGCAATTTGCCAAGCTCCTGAAGCAGAAGAGGATCACCCTGGGATATACACAGGCCG
  ATGTGGGGCTCACCCTGGGGGTTCTATTTGGGAAGGTATTCAGCCAAACGACCATCTGCC
  GCTTTGAGGCTCTGCAGCTTAGCTTCAAGAACATGTGTAAGCTGCGGCCCTTGCTGCAGA
  AGTGGGTGGAGGAAGCTGACAACAATGAAAATCTTCAGGAGATATGCAAAGCAGAAACCC
  TCGTGCAGGCCCGAAAGAGAAAGCGAACCAGTATCGAGAACCGAGTGAGAGGCAACCTGG
  AGAATTTGTTCCTGCAGTGCCCGAAACCGACACTGCAGCAGATCAGCCACATCGCCCAGC
  AGCTTGGGCTCGAGAAGGATGTGGTCCGAGTGTGGTTCTGTAACCGGCGCCAGAAGGGCA
  AGCGATCAAGCAGCGACTATGCACAACGAGAGGATTTTGAGGCTGCTGGGTCTCCTTTCT
  CAGGGGGACCAGTGTCCTTTCCTCTGGCCCCAGGGCCCCATTTTGGTACCCCAGGCTATG
  GGAGCCCTCACTTCACTGCACTGTACTCCTCGGTCCCTTTCCCTGAGGGGGAAGCCTTTC
  CCCCTGTCTCCGTCACCACTCTGGGCTCTCCCATGCATTCAAACTGAGGTGCCTGCCCTT
  CTAGGAATGGGGGACAGGGGGAGGGGAGGAGCTAGGGAAAGAAAACCTGGAGTTTGTGCC
  AGGGTTTTTGGGATTAAGTTCTTCATTCACTAAGGAAGGAATTGGGAACACAAAGGGTGG
  GGGCAGGGGAGTTTGGGGCAACTGGTTGGAGGGAAGGTGAAGTTCAATGATGCTCTTGAT
  TTTAATCCCACATCATGTATCACTTTTTTCTTAAATAAAGAAGCCTGGGACACAGTAGAT
  AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' genomic read for NM_002701 unedited
  ACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCGGGGTGGCTGGATTTGGCCAGTATC
  GGGATGGGAATGCCTAGGATTCTGGATGGATCGGGGGAAGGCATAAGGGAGCAGCTGGCC
  ATTGTGCTTATGGCTGTTGATGCATTGAGGGATAGCGCCACACACACATTCAATAAATTT
  GAGGAGCTGAGAGGGTGACTGGCCCCTGAAGGCACAGTGCCAGAGGTCTGTGGAGAGGGG
  GTCAAGCACCTGGGTTCCTGAAGAACATGGAGGTGTGGGAGTGATTCCAGACAGCTGGGA
  TGTGCAGAGCCTGAGAGAGTGCCAGGGAGCGGGTTGGGAGTTGAAAGTTGGGTGTGGTGG
  CTCACGCCTTTAATCATGACACTGGGCGGCAGAGGCGGGAGGATTTCTTGAGGACAGGAA
  TTCAAGACCAGCCTGGGTAACATAGCAAGGCCCCATCTCTACTAAAAATAAAAAAACTAA
  CAGGGCACAGTGGTCCAAGCCTGTAGTCCCAGCCACTTAGGAGGCTGGAGCAGAAGGATT
  GCTTTGGCCCAGTAGATCGAGGCTACATTGAGCCATCATTGTACTCCACTGCACTCCAGT
  CTGGGCAACAAAGTGAGACCCTGTCTTANAAAATAAAAATAAAAAAAGTTTCTGTGGNGG
  ACCTGCACTGAGGTCCTGGAGGGGCGCCAGTTGTGTCTCCCGGTTTTCCCCTTCCACAGA
  CACCATTGCCACCACCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002701 unedited
  CCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAG
  CTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC
  TATCTACTGTGTCCCAGGCTTCTTTATTTAAGAAAAAAGTGATACATGATGTGGGATTAA
  AATCAAGAGCATCATTGAACTTCACCTTCCCTCCAACCAGTTGCCCCAAACTCCCCTGCC
  CCCACCCTTTGTGTTCCCAATTCCTTCCTTAGTGAATGAAGAACTTAATCCCAAAAACCC
  TGGCACAAACTCCAGGTTTTCTTTCCCTAGCTCCTCCCCTCCCCCTGTCCCCCATTCCTA
  GAAGGGCAGGCACCTCAGTTTGAATGCATGGGAGAGCCCAGAGTGGTGACGGAGACAGGG
  GGAAAGGCTTCCCCCTCAGGGAAAGGGACCGAGGAGTACAGTGCAGTGAAGTGAGGGCTC
  CCATAGCCTGGGGTACCAAAATGGGGCCCTGGGGCCAGAGGAAAGGACACTGGTCCCCCT
  GAGAAAGGAGACCCAGCAGCCTCAAAATCCTCTCGTTGTGCATAGTCGCTGCTTGATCGC
  TTGCCCTTCTGGCGCCGGTTACAGAACCACACTCGGACCACATCCTTCTCGAGCCCAAGC
  TGCTGGGCGATGTGGCTGATCTGCTGCAGTGTCGGTTTCGGGCACTGCAGGAACAAAATC
  TCCAGGTTGCCTCTCACTCGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002701
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002701.3, NP_002692.2
• RefSeq Size: 1417 bp
• RefSeq ORF: 1083 bp
• Locus ID: 5460
• UniProt ID: Q01860
• Cytogenetics: 6p21.33
• Protein Families: Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]
  Transcript Variant: This variant (1, also known as OCT4A) represents the shortest transcript and encodes the longest isoform (1).