Oct4 (POU5F1) (NM_002701) Human Untagged Clone

SKU SC125252

POU5F1/OCT4 (untagged)-Human POU class 5 homeobox 1 (POU5F1/OCT4), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Oct4
• Synonyms: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002701 edited; CTGCGGGGTGGCTGGATTTGGCCAGTATCGGGATGGGAATGCCTAGGATTCTGGATGGAT; CGGGGGAAGGCATAAGGGAGCAGCTGGCCATTGTGCTTATGGCTGTTGATGCATTGAGGG; ATAGCGCCACACACACATTCAATAAATTTGAGGAGCTGAGAGGGTGACTGGCCCCTGAAG; GCACAGTGCCAGAGGTCTGTGGAGAGGGGGTCAAGCACCTGGGTTCCTGAAGAACATGGA; GGTGTGGGAGTGATTCCAGACAGCTGGGATGTGCAGAGCCTGAGAGAGTGCCAGGGAGCG; GGTTGGGAGTTGAAAGTTGGGTGTGGTGGCTCACGCCTTTAATCATGACACTGGGCGGCA; GAGGCGGGAGGATTTCTTGAGGACAGGAATTCAAGACCAGCCTGGGTAACATAGCAAGGC; CCCATCTCTACTAAAAATAAAAAAACTAACAGGGCACAGTGGTCCAAGCCTGTAGTCCCA; GCCACTTAGGAGGCTGGAGCAGAAGGATTGCTTTGGCCCAGTAGATCGAGGCTACATTGA; GCCATCATTGTACTCCACTGCACTCCAGTCTGGGCAACAAAGTGAGACCCTGTCTTAAAA; AATAAAAATAAAAAAAGTTTCTGTGGGGGACCTGCACTGAGGTCCTGGAGGGGCGCCAGT; TGTGTCTCCCGGTTTTCCCCTTCCACAGACACCATTGCCACCACCATTAGGCAAACATCC; TTCGCCTCAGTTTCTCCCCCCACCTCCCTCTCCTCCACCCATCCAGGGGGCGGGGCCAGA; GGTCAAGGCTAGTGGGTGGGACTGGGGAGGGAGAGAGGGGTTGAGTAGTCCCTTCGCAAG; CCCTCATTTCACCAGGCCCCCGGCTTGGGGCGCCTTCCTTCCCCATGGCGGGACACCTGG; CTTCGGATTTCGCCTTCTCGCCCCCTCCAGGTGGTGGAGGTGATGGGCCAGGGGGGCCGG; AGCCGGGCTGGGTTGATCCTCGGACCTGGCTAAGCTTCCAAGGCCCTCCTGGAGGGCCAG; GAATCGGGCCGGGGGTTGGGCCAGGCTCTGAGGTGTGGGGGATTCCCCCATGCCCCCCGC; CGTATGAGTTCTGTGGGGGGATGGCGTACTGTGGGCCCCAGGTTGGAGTGGGGCTAGTGC; CCCAAGGCGGCTTGGAGACCTCTCAGCCTGAGGGCGAAGCAGGAGTCGGGGTGGAGAGCA; ACTCCGATGGGGCCTCCCCGGAGCCCTGCACCGTCACCCCTGGTGCCGTGAAGCTGGAGA; AGGAGAAGCTGGAGCAAAACCCGGAGGAGTCCCAGGACATCAAAGCTCTGCAGAAAGAAC; TCGAGCAATTTGCCAAGCTCCTGAAGCAGAAGAGGATCACCCTGGGATATACACAGGCCG; ATGTGGGGCTCACCCTGGGGGTTCTATTTGGGAAGGTATTCAGCCAAACGACCATCTGCC; GCTTTGAGGCTCTGCAGCTTAGCTTCAAGAACATGTGTAAGCTGCGGCCCTTGCTGCAGA; AGTGGGTGGAGGAAGCTGACAACAATGAAAATCTTCAGGAGATATGCAAAGCAGAAACCC; TCGTGCAGGCCCGAAAGAGAAAGCGAACCAGTATCGAGAACCGAGTGAGAGGCAACCTGG; AGAATTTGTTCCTGCAGTGCCCGAAACCGACACTGCAGCAGATCAGCCACATCGCCCAGC; AGCTTGGGCTCGAGAAGGATGTGGTCCGAGTGTGGTTCTGTAACCGGCGCCAGAAGGGCA; AGCGATCAAGCAGCGACTATGCACAACGAGAGGATTTTGAGGCTGCTGGGTCTCCTTTCT; CAGGGGGACCAGTGTCCTTTCCTCTGGCCCCAGGGCCCCATTTTGGTACCCCAGGCTATG; GGAGCCCTCACTTCACTGCACTGTACTCCTCGGTCCCTTTCCCTGAGGGGGAAGCCTTTC; CCCCTGTCTCCGTCACCACTCTGGGCTCTCCCATGCATTCAAACTGAGGTGCCTGCCCTT; CTAGGAATGGGGGACAGGGGGAGGGGAGGAGCTAGGGAAAGAAAACCTGGAGTTTGTGCC; AGGGTTTTTGGGATTAAGTTCTTCATTCACTAAGGAAGGAATTGGGAACACAAAGGGTGG; GGGCAGGGGAGTTTGGGGCAACTGGTTGGAGGGAAGGTGAAGTTCAATGATGCTCTTGAT; TTTAATCCCACATCATGTATCACTTTTTTCTTAAATAAAGAAGCCTGGGACACAGTAGAT; AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' genomic read for NM_002701 unedited; ACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCGGGGTGGCTGGATTTGGCCAGTATC; GGGATGGGAATGCCTAGGATTCTGGATGGATCGGGGGAAGGCATAAGGGAGCAGCTGGCC; ATTGTGCTTATGGCTGTTGATGCATTGAGGGATAGCGCCACACACACATTCAATAAATTT; GAGGAGCTGAGAGGGTGACTGGCCCCTGAAGGCACAGTGCCAGAGGTCTGTGGAGAGGGG; GTCAAGCACCTGGGTTCCTGAAGAACATGGAGGTGTGGGAGTGATTCCAGACAGCTGGGA; TGTGCAGAGCCTGAGAGAGTGCCAGGGAGCGGGTTGGGAGTTGAAAGTTGGGTGTGGTGG; CTCACGCCTTTAATCATGACACTGGGCGGCAGAGGCGGGAGGATTTCTTGAGGACAGGAA; TTCAAGACCAGCCTGGGTAACATAGCAAGGCCCCATCTCTACTAAAAATAAAAAAACTAA; CAGGGCACAGTGGTCCAAGCCTGTAGTCCCAGCCACTTAGGAGGCTGGAGCAGAAGGATT; GCTTTGGCCCAGTAGATCGAGGCTACATTGAGCCATCATTGTACTCCACTGCACTCCAGT; CTGGGCAACAAAGTGAGACCCTGTCTTANAAAATAAAAATAAAAAAAGTTTCTGTGGNGG; ACCTGCACTGAGGTCCTGGAGGGGCGCCAGTTGTGTCTCCCGGTTTTCCCCTTCCACAGA; CACCATTGCCACCACCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002701 unedited; CCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAG; CTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC; TATCTACTGTGTCCCAGGCTTCTTTATTTAAGAAAAAAGTGATACATGATGTGGGATTAA; AATCAAGAGCATCATTGAACTTCACCTTCCCTCCAACCAGTTGCCCCAAACTCCCCTGCC; CCCACCCTTTGTGTTCCCAATTCCTTCCTTAGTGAATGAAGAACTTAATCCCAAAAACCC; TGGCACAAACTCCAGGTTTTCTTTCCCTAGCTCCTCCCCTCCCCCTGTCCCCCATTCCTA; GAAGGGCAGGCACCTCAGTTTGAATGCATGGGAGAGCCCAGAGTGGTGACGGAGACAGGG; GGAAAGGCTTCCCCCTCAGGGAAAGGGACCGAGGAGTACAGTGCAGTGAAGTGAGGGCTC; CCATAGCCTGGGGTACCAAAATGGGGCCCTGGGGCCAGAGGAAAGGACACTGGTCCCCCT; GAGAAAGGAGACCCAGCAGCCTCAAAATCCTCTCGTTGTGCATAGTCGCTGCTTGATCGC; TTGCCCTTCTGGCGCCGGTTACAGAACCACACTCGGACCACATCCTTCTCGAGCCCAAGC; TGCTGGGCGATGTGGCTGATCTGCTGCAGTGTCGGTTTCGGGCACTGCAGGAACAAAATC; TCCAGGTTGCCTCTCACTCGN;
• Restriction Sites: NotI-NotI
• ACCN: NM_002701
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002701.3, NP_002692.2
• RefSeq Size: 1417 bp
• RefSeq ORF: 1083 bp
• Locus ID: 5460
• UniProt ID: Q01860
• Cytogenetics: 6p21.33
• Protein Families: Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors
• Summary: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]; Transcript Variant: This variant (1, also known as OCT4A) represents the shortest transcript and encodes the longest isoform (1).