MRPL36 (NM_032479) Human Untagged Clone

SKU
SC124962
MRPL36 (untagged)-Human mitochondrial ribosomal protein L36 (MRPL36), nuclear gene encoding mitochondrial protein
$150.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MRPL36
Synonyms BRIP1; L36mt; MRP-L36; PRPL36; RPMJ
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC124962 sequence for NM_032479 edited (data generated by NextGen Sequencing)
ATGGCAAATCTTTTTATAAGGAAAATGGTGAACCCTCTGCTCTATCTCAGTCGTCACACG
GTGAAGCCTCGAGCCCTCTCCACATTTCTATTTGGATCCATTCGAGGTGCAGCCCCCGTG
GCTGTGGAACCCGGGGCAGCAGTGCGCTCACTTCTCTCACCCGGCCTCCTGCCCCATCTG
CTGCCTGCGCTGGGGTTCAAAAACAAGACTGTCCTTAAGAAGCGCTGCAAGGACTGTTAC
CTGGTGAAGAGGCGGGGTCGGTGGTACGTCTACTGTAAAACCCATCCGAGGCACAAGCAG
AGACAGATGTAG

Clone variation with respect to NM_032479.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_032479 unedited
GCCGCGAATTCGGCACGAGCGCCGGGTGAGAGNCGTGCGGCCGGATTCACCACAACATGG
CAAATCTTTTTATAAGGAAAATGGTGAACCCTCTGCTCTATCTCAGTCGTCACACGGTGA
AGCCTCGAGCCCTCTCCACATTTCTATTTGGATCCATTCGAGGTGCAGCCCCCGTGGCTG
TGGAACCCGGGGCAGCAGTGCGCTCACTTCTCTCACCCGGCCTCCTGCCCCATCTGCTGC
CTGCGCTGGGGTTCAAAAACAAGACTGTCCTTAAGAAGCGCTGCAAGGACTGTTACCTGG
TGAAGAGGCGGGGTCGGTGGTACGTCTACTGTAAAACCCATCCGAGGCACAAGCAGAGAC
AGATGTAGACCCTTTCCCTCCAGAGTCACGCACATACTCGTCATCGCATCACTTGGGAGA
ATGGTTGTATCTTATGGAAGGAATTATCACATCAAGGAGTCAGGGGAANAGTGACTGGAA
GCAAACGCCCTAAAAGTTACCCATCACGTTTCAGTGTAAATGAGTAACTATAGAAGACAT
TGCGTTATCTTATTTCCAAAACGTTCCAACTAAAAACATTTTCCTATTAAATTAGACCTT
CCGAAAAAAAAAAAAAAAAAACTCACTCTAGATTGCGGCCGCGGCATAGCTGGTTCTGAA
CAATCCCCGGTGGCATCCCTGTGGACCTTCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCC
CCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_032479 unedited
NTTCGTTTACTAGNACCGCGCCGCATCTANATCGATTTTTTTTTTTTTTTTTTTTCGAGG
TCTATTTAATAGGAAAAGTTTTTTAGTTGGAACGTTTTGGAAATAAGATAACGCAATGTC
TTCTATAGTTACTCATTTACACTGAAACGTGATGGGTAACTTTTAGGGCGTTTGCTTCCA
GTCACTTTCCCCTGACTCCTTGATGTGATAATTCCTTCCATAAGATACAACCATTCTCCC
AAGTGATGCGATGACGAGTATGTGCGTGACTCTGGAGGGAAAGGGTCTACATCTGTCTCT
GCTTGTGCCTCGGATGGGTTTTACAGTAGACGTACCACCGACCCCGCCTCTTCACCAGGT
AACAGTCCTTGCAGCGCTTCTTAAGGACAGTCTTGTTTTTGAACCCCAGCGCAGGCAGCA
GATGGGGCAGGAGGCCGGGTGAGAGAAGTGAGCGCACTGCTGCCCCGGGTTCCACAGCCA
CGGGGGCTGCACCTCGAATGGATCCAAATAGAAATGTGGAGAGGGCTCGAGGCTTCACCG
TGTGACGACTGAGATAGAGCAGAGGGTTCACCATTTTCCTTATAAAAAGATTTGCCATGT
TGTGGTGAATCCGGCCGCACGCTCTCACCCGGCGCTCGTGCCGAATTCGCGGCCGCCCTA
TAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCT
CCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTG
GAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGGAGA
CTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGCCAAAACGCA
TCACATGGC
Restriction Sites NotI-NotI
ACCN NM_032479
Insert Size 640 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032479.2, NP_115868.1
RefSeq Size 604 bp
RefSeq ORF 312 bp
Locus ID 64979
UniProt ID Q9P0J6
Cytogenetics 5p15.33
Domains Ribosomal_L36
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 2p. [provided by RefSeq, Jul 2008]
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Citations

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