NRG1 (NM_013956) Human Untagged Clone

SKU SC124810

NRG1 (untagged)-Human neuregulin 1 (NRG1), transcript variant HRG-beta1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NRG1
• Synonyms: ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; NRG1-IT2; SMDF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124810 sequence for NM_013956 edited (data generated by NextGen Sequencing); ATGTCCGAGCGCAAAGAAGGCAGAGGCAAAGGGAAGGGCAAGAAGAAGGAGCGAGGCTCC; GGCAAGAAGCCGGAGTCCGCGGCGGGCAGCCAGAGCCCAGCCTTGCCTCCCCAATTGAAA; GAGATGAAAAGCCAGGAATCGGCTGCAGGTTCCAAACTAGTCCTTCGGTGTGAAACCAGT; TCTGAATACTCCTCTCTCAGATTCAAGTGGTTCAAGAATGGGAATGAATTGAATCGAAAA; AACAAACCACAAAATATCAAGATACAAAAAAAGCCAGGGAAGTCAGAACTTCGCATTAAC; AAAGCATCACTGGCTGATTCTGGAGAGTATATGTGCAAAGTGATCAGCAAATTAGGAAAT; GACAGTGCCTCTGCCAATATCACCATCGTGGAATCAAACGAGATCATCACTGGTATGCCA; GCCTCAACTGAAGGAGCATATGTGTCTTCAGAGTCTCCCATTAGAATATCAGTATCCACA; GAAGGAGCAAATACTTCTTCATCTACATCTACATCCACCACTGGGACAAGCCATCTTGTA; AAATGTGCGGAGAAGGAGAAAACTTTCTGTGTGAATGGAGGGGAGTGCTTCATGGTGAAA; GACCTTTCAAACCCCTCGAGATACTTGTGCAAGTGCCCAAATGAGTTTACTGGTGATCGC; TGCCAAAACTACGTAATGGCCAGCTTCTACAAGCATCTTGGGATTGAATTTATGGAGGCG; GAGGAGCTGTACCAGAAGAGAGTGCTGACCATAACCGGCATCTGCATCGCCCTCCTTGTG; GTCGGCATCATGTGTGTGGTGGCCTACTGCAAAACCAAGAAACAGCGGAAAAAGCTGCAT; GACCGTCTTCGGCAGAGCCTTCGGTCTGAACGAAACAATATGATGAACATTGCCAATGGG; CCTCACCATCCTAACCCACCCCCCGAGAATGTCCAGCTGGTGAATCAATACGTATCTAAA; AACGTCATCTCCAGTGAGCATATTGTTGAGAGAGAAGCAGAGACATCCTTTTCCACCAGT; CACTATACTTCCACAGCCCATCACTCCACTACTGTCACCCAGACTCCTAGCCACAGCTGG; AGCAACGGACACACTGAAAGCATCCTTTCCGAAAGCCACTCTGTAATCGTGATGTCATCC; GTAGAAAACAGTAGGCACAGCAGCCCAACTGGGGGCCCAAGAGGACGTCTTAATGGCACA; GGAGGCCCTCGTGAATGTAACAGCTTCCTCAGGCATGCCAGAGAAACCCCTGATTCCTAC; CGAGACTCTCCTCATAGTGAAAGGTATGTGTCAGCCATGACCACCCCGGCTCGTATGTCA; CCTGTAGATTTCCACACGCCAAGCTCCCCCAAATCGCCCCCTTCGGAAATGTCTCCACCC; GTGTCCAGCATGACGGTGTCCATGCCTTCCATGGCGGTCAGCCCCTTCATGGAAGAAGAG; AGACCTCTACTTCTCGTGACACCACCAAGGCTGCGGGAGAAGAAGTTTGACCATCACCCT; CAGCAGTTCAGCTCCTTCCACCACAACCCCGCGCATGACAGTAACAGCCTCCCTGCTAGC; CCCTTGAGGATAGTGGAGGATGAGGAGTATGAAACGACCCAAGAGTACGAGCCAGCCCAA; GAGCCTGTTAAGAAACTCGCCAATAGCCGGCGGGCCAAAAGAACCAAGCCCAATGGCCAC; ATTGCTAACAGATTGGAAGTGGACAGCAACACAAGCTCCCAGAGCAGTAACTCAGAGAGT; GAAACAGAAGATGAAAGAGTAGGTGAAGATACGCCTTTCCTGGGCATACAGAACCCCCTG; GCAGCCAGTCTTGAGGCAACACCTGCCTTCCGCCTGGCTGACAGCAGGACTAACCCAGCA; GTCCGCTTCTCGACACAGGAAGAAATCCAGGCCAGGCTGTCTAGTGTAATTGCTAACCAA; GACCCTATTGCTGTATAA; ; Clone variation with respect to NM_013956.3; 113 g=>a;1862 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013956 unedited; ATACGACTCCTATAGGGCGGCCGCGAATTCGCCGAGGGGCTCGCGCGGAGGCCAGGAGCT; GAGCGGCGGCGGCTGCCGGACGATGGGAGCGTGAGCAGGACGGTGATAACCTCTCCCCGA; TCGGGTTGCGAGGGCGCCGGGCAGAGGCCAGGACGCGAGCCGCCAGCGGCGGGACCCATC; GACGACTTCCCGGGGCGACAGGAGCAGCCCCGAGAGCCAGGGCGAGCGCCCGTTCCAGGT; GGCCGGACCGCCCGCCGCGTCCGCGCCGCGCTCCCTGCAGGCAACGGGAGACGCCCCCGC; GCAGCGCGAGCGCCTCAGCGCGGCCGCTCGCTCTCCCCATCGAGGGACAAACTTTTCCCA; AACCCGATCCGAGCCCTTGGACCAAACTCGCCTGCGCCGAGAGCCGTCCGCGTAGAGCGC; TCCGTCTCCGGCGAGATGTCCGAGCGCAAAGAAGGCAGAGGCAAAGGGAAGGGCAAGAAG; AAGGAGCGAGGCTCCGGCAAGAAGCCGGAGTCCGCGGCGGGCAGCCAGAGCCCAGCCTTG; CCTCCCCAATTGAAAGAGATGAAAAGCCAGGAATCGGCTGCAGGTTCCAAACTAGTCCTT; CGGTGTGAAACCAGTTCTGAATACTCCTCTCTCAGATTCAAGTGGTTCAAGAATGGGAAT; GAATTGAATCGAANAAACAAACCACAAAATATCAAGATACAAAAAAAGCCAGNGGAAGTC; AGAACTTCGCATTAACAAAGCATCACTGGCTGATTCTGGAGAGTATATGTGCANAGTGAT; CAGCAATTAGGAAATGACAGTGCCTCTGCCATATCACCATCGTGGATCAAACGAGATCTC; ACTGGTATGCCAGNCTCACTGAAGAGCCTAATGTGCT;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_013956 unedited; NTTCTCCGCGAAAAAATCAGGCACANCAGCCCACTGGGGGCCCAAAAGGACGTCTTAATG; GCACAGGAGGCCCTNCGTGATGTAACAGCTTCCTCAGGCATGCCAGAGAAACCCCTGATT; CCTACCGAGACTCTCCTCATAGTGAAAGGTATGTGTCAGCCATGACCACCCCGGCTCGTA; TGTCACCTGTAGATTTCCACACGCCAAGCTCCCCCAAATCGCCCCCTTCGGAAATGTCTC; CACCCGTGTCCAGCATGACGGTGTCCATGCCTTCCATGGCGGTCAGCCCCTTCATGGAAG; AAGAGAGACCTCTACTTCTCGTGACACCACCAAGGCTGCGGGAGAAGAAGTTTGACCATC; ACCCTCAGCAGTTCAGCTCCTTCCACCACAACCCCGCGCATGACAGTAACAGCCTCCCTG; CTAGCCCCTTGAGGATAGTGGAGGATGAGGAGTATGAAACGACCCAAGAGTACGAGCCAG; CCCAAGAGCCTGTTAAGAAACTCGCCAATAGCCGGCGGGCCAAAAGACCAAGCCCAATGG; CCACATTGCTAACAGATTGGAAGTGGACAGCAACACAAGCTCCCAGAGCAGTAACTCAGA; GAGTGAAACAGAAGATGAAAGAGTAGGTGAAGATACGCCTTTCCTGGGCATACAGAACCC; CCTGGCAGCCAGTCTTGAGGCAACACCTGCCTTCCGCCTGGCTGACAGCAGGACTAACCC; CGCAGTTCCGCTTCTCGACACAGGAAAAAATCCAGGCCACAGCTGGCTAGTGGTAATTGG; TAACCCAAAAACCCTTTTGCTGGTTTAAAACCTAAATAAACCCCTTGGATTTCCCTGGGA; AAACTTTATTTTTATTTTAAAAAGGTTTTTCCCCTCTAAAATAAAAACAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_013956
• Insert Size: 4100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013956.2, NP_039250.2
• RefSeq Size: 2583 bp
• RefSeq ORF: 1938 bp
• Locus ID: 3084
• UniProt ID: Q02297
• Cytogenetics: 8p12
• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: ErbB signaling pathway
• Summary: The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Apr 2016]; Transcript Variant: This variant (HRG-beta1), which uses the type I promoter, encodes isoform HRG-beta1 (also known as NDF-beta1a or HRG-beta1a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.