CD57 (B3GAT1) (NM_054025) Human Untagged Clone

SKU SC124786

B3GAT1 (untagged)-Human beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3GAT1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: CD57
• Synonyms: CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_054025, the custom clone sequence may differ by one or more nucleotides; ; ; ATGCCGAAGAGACGGGACATCCTAGCGATCGTCCTCATCGTGCTGCCCTGGACTCTGCTCATCACTGTCT; GGCACCAGAGCACCCTCGCACCCCTGCTCGCGGTACATAAGGATGAGGGCAGTGACCCCCGACGCGAAAC; GCCGCCCGGCGCCGACCCCAGGGAGTACTGCACGTCTGACCGCGACATCGTGGAGGTGGTGCGCACCGAG; TACGTGTACACGCGGCCCCCGCCATGGTCCGACACGCTGCCCACCATCCACGTGGTGACGCCCACCTACA; GCCGCCCGGTGCAGAAGGCCGAGCTGACGCGCATGGCCAACACGCTGCTGCACGTGCCCAACCTCCACTG; GCTGGTGGTGGAGGATGCGCCGCGCCGGACGCCGCTGACCGCGCGCCTGCTGCGCGACACCGGCCTCAAC; TACACGCACCTGCACGTGGAGACGCCCCGCAACTACAAGCTGCGCGGAGACGCCCGCGACCCACGCATCC; CGCGGGGCACCATGCAGCGCAACCTGGCCCTGCGCTGGCTGCGCGAGACCTTCCCGCGCAACTCCAGCCA; GCCTGGCGTGGTCTACTTCGCCGACGACGACAACACCTACAGCCTGGAGCTCTTCGAAGAGATGCGCAGC; ACCAGGAGGGTGTCCGTGTGGCCCGTCGCCTTCGTGGGTGGCCTGCGGTACGAGGCCCCACGGGTGAACG; GGGCAGGGAAGGTGGTCGGCTGGAAGACGGTGTTTGACCCCCACCGGCCATTTGCAATAGACATGGCTGG; ATTTGCCGTCAACCTGCGGCTCATTCTGCAGCGAAGCCAGGCCTACTTCAAGCTGCGAGGTGTGAAGGGA; GGCTACCAGGAAAGCAGCCTCCTTCGAGAACTTGTCACCCTCAACGACCTGGAGCCCAAGGCAGCCAACT; GCACCAAGATCCTGGTGTGGCACACACGGACAGAGAAGCCAGTGCTGGTGAATGAGGGCAAGAAGGGCTT; CACTGACCCCTCGGTGGAGATCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_054025 unedited; TGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACCCCCCAGGTTCC; TGACCCCTGCCCCTGGACAGCGACCCCTTCTCAGACTCCAGTTGGGCCGGACTCTCCAAA; CCTGCTTCCGCAATGGGTAATGAGGAGCCGTGGGTGCAGCCAGCCTTGGAGATGCCGAAG; AGACGGGACATCCTAGCGATCGTCCTCATCGTGCTGCCCTGGACTCTGCTCATCACTGTC; TGGCACCAGAGCACCCTCGCACCCCTGCTCGCGGTACATAAGGATGAGGGCAGTGACCCC; CGACGCGAAACGCCGCCCGGCGCCGACCCCAGGGAGTACTGCACGTCTGACCGCGACATC; GTGGAGGTGGTGCGCACCGAGTACGTGTACACGCGGCCCCCGCCATGGTCCGACACGCTG; CCCACCATCCACGTGGTGACGCCCACCTACAGCCGCCCGGTGCAGAAGGCCGAGCTGACG; CGCATGGCCAACACGCTGCTGCACGTGCCCAACCTCCACTGGCTGGTGGTGGAGGATGCG; CCGCGCCGGACGCCGTTGACCGCGCGCCTGCTGCGCGACACCGGCCTCAACTACACGCAC; CTGCACGTGGAGACGCCCCGCAACTACAAGCTGCGCGGAGACGCCCGCGACCCACGCATC; CCGCGGGGCACCATGCAGCGCAAACCTGGCCCTGCGCTGGCTGGGCGAGACCTTCCGCGC; AACTCCACCAGCTTGGGGTGGTCTACTTTGCCGACGACAACAAAACTTAAGCCTGGAACT; TTTNAAGAGATGCCCCACACCAGGAGGGGTCCCGGGGGGCCCGTCCCCTTCGGGGTGGCC; CGGGGAACAAGCCCCAACGGTGAACCGGGCATGGTAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_054025
• Insert Size: 3620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_054025.1, NP_473366.1
• RefSeq Size: 3666 bp
• RefSeq ORF: 3666 bp
• Locus ID: 27087
• UniProt ID: Q9P2W7
• Cytogenetics: 11q25
• Domains: Glyco_transf_43
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways
• Summary: The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) has multiple differences in the 5' end of the transcript, as compared to variant 1. Variants 1 and 2 encode the same protein.