CUG BP1 (CELF1) (NM_198700) Human Untagged Clone

SKU SC124753

CELF1 (untagged)-Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: CUG BP1
• Synonyms: BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP; hNab50; NAB50; NAPOR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_198700, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAACGGCACCCTGGACCACCCAGACCAACCAGATCTTGATGCTATCAAGATGTTTGTGGGCCAGGTTC; CAAGGACCTGGTCTGAAAAGGACTTGCGGGAACTCTTCGAACAGTATGGTGCTGTGTATGAAATCAACGT; CCTAAGGGATAGGAGCCAAAACCCGCCTCAGAGCAAAGGGTGCTGTTTTGTTACATTTTACACCCGTAAA; GCTGCATTAGAAGCTCAGAATGCTCTTCACAACATGAAAGTCCTCCCAGGGATGCATCACCCTATACAGA; TGAAACCTGCTGACAGTGAGAAGAACAATGCAGTGGAAGACAGGAAGCTGTTTATTGGTATGATTTCCAA; GAAGTGCACTGAAAATGACATCCGAGTCATGTTCTCTTCGTTTGGACAGATTGAAGAATGCCGGATATTG; CGGGGACCTGATGGCCTGAGCCGAGGTTGTGCATTTGTGACTTTTACAACAAGAGCCATGGCACAGACGG; CTATCAAGGCAATGCACCAAGCACAGACCATGGAGGGTTGCTCATCACCCATGGTGGTAAAATTTGCTGA; TACACAGAAGGACAAAGAACAGAAGAGAATGGCCCAGCAGCTCCAGCAGCAGATGCAGCAAATCAGCGCA; GCATCTGTGTGGGGAAACCTTGCTGGTCTAAATACTCTTGGACCCCAGTATTTAGCACTCCTTCAGCAGA; CTGCCTCCTCTGGGAACCTCAACACCCTGAGCAGCCTCCACCCAATGGGAGGGTTGAATGCAATGCAGTT; ACAGAATTTGGCTGCACTAGCTGCTGCAGCTAGTGCAGCTCAGAACACACCAAGTGGTACCAATGCTCTC; ACTACATCCAGCAGTCCCCTCAGCGTGCTCACTAGTTCAGCAGGGTCCTCACCTAGCTCTAGCAGCAGTA; ATTCTGTCAACCCCATAGCCTCACTTGGAGCCCTGCAGACATTAGCTGGAGCAACGGCTGGCCTCAATGT; TGGCTCTTTGGCAGGAATGGCTGCTTTAAATGGTGGCCTGGGCAGCAGTGGCCTTTCCAATGGCACCGGG; AGCACCATGGAGGCCCTCACTCAGGCCTACTCGGGTATCCAGCAATATGCTGCTGCTGCGCTCCCCACTC; TGTACAACCAGAATCTTCTGACACAGCAGAGTATTGGTGCTGCTGGAAGCCAGAAGGAAGGTCCAGAGGG; AGCCAACCTGTTCATCTACCACCTGCCCCAGGAGTTTGGTGATCAGGACCTGCTGCAGATGTTTATGCCC; TTTGGGAATGTCGTGTCTGCCAAGGTTTTCATAGACAAGCAGACAAACCTGAGCAAGTGTTTTGGTTTTG; TAAGTTACGACAATCCTGTTTCGGCCCAAGCTGCCATCCAGTCCATGAACGGCTTTCAGATTGGCATGAA; GCGGCTTAAAGTGCAGCTCAAACGTTCGAAGAATGACAGCAAGCCCTACTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198700 unedited; ATATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCAAAGAAA; ATGAACGGCACCCTGGACCACCCAGACCAACCAGATCTTGATGCTATCAAGATGTTTGTG; GGCCAGGTTCCAAGGACCTGGTCTGAAAAGGACTTGCGGGAACTCTTCGAACAGTATGGT; GCTGTGTATGAAATCAACGTCCTAAGGGATAGGAGCCAAAACCCGCCTCAGAGCAAAGGG; TGCTGTTTTGTTACATTTTACACCCGTAAAGCTGCATTAGAAGCTCAGAATGCTCTTCAC; AACATGAAAGTCCTCCCAGGGATGCATCACCCTATACAGATGAAACCTGCTGACAGTGAG; AAGAACAATGCAGTGGAAGACAGGAAGCTGTTTATTGGTATGATTTCCAAGAAGTGCACT; GAAAATGACATCCGAGTCATGTTCTCTTCGTTTGGACAGATTGAAGAATGCCGGATATTG; CGGGGACCTGATGGCCTGAGCCGAGGTTGTGCATTTGTGACTTTTACAACAAGAGCCATG; GCACAGACGGCTATCAAGGCAATGCACCAAGCACAGAACCATGGAGGGTTGCTCATCACC; CATGGTGGTAAAATTTGCTGATACACAGAAGGACAAAGAACAGAAGAGAATGGCCCAGCA; GCTCCAGCAGCAGATGCAGCAAATCAGCGCAGCATTCTGTGTGGGGAAACCTTGCTGGTC; TAAATACTCTTGGACCCCAGTATTTAGCACTTTATTTGCAGCTTCTTCAGCAGACTGCCT; CCTCTGGGAACCCTCACACCTGAGCAGNCTCCACCATGGGGGAGGGNTGAATGCATGCAG; TACAGAATTTGGCTGCACTAACTGCTGCAGCTAGTGCAGCTCAGACACAACCAAGTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_198700
• Insert Size: 3370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_198700.1, NP_941989.1
• RefSeq Size: 2206 bp
• RefSeq ORF: 1452 bp
• Locus ID: 10658
• UniProt ID: Q92879
• Cytogenetics: 11p11.2
• Protein Families: Druggable Genome
• Summary: Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) uses a different segment for its 5' UTR and a different internal splice site in its coding region, compared to variant 1. The resulting isoform (2) has an additional internal amino acid compared to isoform 1.