NIPP1 (PPP1R8) (NM_002713) Human Untagged Clone

CAT#: SC124701

PPP1R8 (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NIPP1
• Synonyms: ARD-1; ARD1; NIPP-1; NIPP1; PRO2047
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124701 sequence for NM_002713 edited (data generated by NextGen Sequencing)
  ATGGTGCAAACTGCAGTGGTCCCAGTCAAGAAGAAGCGTGTGGAGGGCCCTGGCTCCCTG
  GGCCTGGAGGAATCAGGGAGCAGGCGCATGCAGAACTTTGCCTTCAGCGGAGGACTCTAC
  GGGGGCCTGCCCCCCACACACAGTGAAGCAGGCTCCCAGCCACATGGCATCCATGGGACA
  GCACTCATCGGTGGCTTGCCCATGCCATACCCAAACCTTGCCCCTGATGTGGACTTGACT
  CCTGTTGTKCCGTCAGCAGTGAACATGAACCCTGCACCAAACCCTGCAGTCTATAACCCT
  GAAGCTGTAAATGAACCCAAGAAGAAGAAATATGCAAAAGAGGCTTGGCCAGGCAAGAAG
  CCCACACCTTCCTTGCTGATTTGA
  
  Clone variation with respect to NM_002713.3
  249 g=>k
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002713 unedited
  NNNCCAGGTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCGG
  CAGCCGCGAACTCCGGCTCTAGCCTCCCGCTGTTCGACTGCCCAACCTGGGCAGGTAAGC
  CCCCTCCCGGTTTACATCTGGATGTAGTCAAAGGAGACAAACTAATTGAGAAACTGATTA
  TTGATGAGAAGAAGTATTACTTATTTGGGAGAAACCCTGATTTGTGTGACTTTACCATTG
  ACCACCAGTCTTGCTCTCGGGTCCATGCTGCACTTGTCTACCACAAGCATCTGAAGAGAG
  TTTTCCTGATAGATCTCAACAGTACACACGGCACTTTCTTGGGTCACATTCGGTTGGAAC
  CTCACAAGCCTCAGCAAATTCCCATCGATTCCACGGTCTCATTTGGCGCATCCACAAGGG
  CATACACTCTGCGCGAGAAGCCTCAGACATTGCCATCGGCTGTGAAAGGAGATGAGAAGA
  TGGGTGGAGAGGATGATGAACTCAAGGGCTTACTGNGGCTTCCAGAGGAGGAAACTGAGC
  TTGATAACCTGACAGAGTTCAACACTGCCCACAACAAAGCGATTTCTACCCTGACCATTG
  ANGAGGNNAATCTGGACATTCAAAGACCANAGAGGAAGAGGAAGAACTCACGGGTGACAT
  TCAGTGAGGATGATGAGATCATCAACCCAGAGATGTGGATCCCTCAGTGGNTCGATTCAG
  GAACATGGNTGCAACTGCAGTGGNTCCAGTCAAGAAAAACGTGTGGNAGGCCCTGGCTCC
  CTGGCCTGNAGAATCANGNACANGCGATGCANAACTTGCCTTAGCAGAGACTCTACGGGG
  CCTGCCCCACACACATGAGCAGCTCCAGCCCATGCATCATGGACACCTCATCGTGGCTGC
  CATGCATACCAANCTGCCTGAGTGACTGACTCCTGTGTCGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002713
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002713.2, NP_002704.1
• RefSeq Size: 2590 bp
• RefSeq ORF: 384 bp
• Locus ID: 5511
• UniProt ID: Q12972
• Cytogenetics: 1p35.3
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) encodes the shortest isoform (gamma, also called ARD-1). It has its first exon longer at the 3' end and lacks an internal exon as compared to that of transcript variant 1. The gamma isoform uses a different translation start site and is N-terminally truncated as compared to isoform alpha encoded by variant 1. Isoform gamma contains ribonuclease activity.