PRKAR1B (NM_002735) Human Untagged Clone

CAT#: SC124688

PRKAR1B (untagged)-Human protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKAR1B
• Synonyms: PRKAR1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124688 sequence for NM_002735 edited (data generated by NextGen Sequencing)
  ATGGCCTCCCCGCCCGCCTGCCCCTCGGAGGAGGACGAGAGCCTGAAGGGCTGTGAGCTG
  TACGTGCAGCTGCACGGGATCCAGCAGGTCCTCAAAGACTGTATCGTCCACCTCTGCATC
  TCCAAGCCCGAACGCCCCATGAAGTTCCTCCGGGAGCACTTCGAGAAGCTGGAGAAGGAA
  GAAAACAGGCAGATTTTGGCGCGGCAAAAGTCAAACTCACAGTCGGACTCCCATGATGAG
  GAGGTGTCGCCCACCCCCCCGAACCCTGTGGTGAAGGCCCGCCGCCGGCGAGGAGGCGTG
  AGTGCCGAGGTGTACACCGAGGAGGACGCCGTGTCCTACGTCAGGAAGGTGATTCCCAAG
  GACTACAAAACCATGACTGCGCTGGCCAAGGCCATCTCCAAGAACGTGCTCTTCGCTCAC
  CTGGATGACAACGAGAGGAGTGACATATTCGATGCCATGTTCCCTGTCACTCACATCGCT
  GGGGAGACTGTTATACAGCAAGGGAATGAAGGAGACAACTTCTATGTCGTTGATCAAGGG
  GAAGTGGATGTGTACGTGAACGGAGAGTGGGTGACCAACATCAGCGAGGGAGGCAGCTTC
  GGGGAGCTGGCGCTCATCTACGGCACCCCCAGGGCTGCGACCGTGAAAGCCAAGACGGAC
  CTCAAGCTCTGGGGGATCGACCGGGACAGCTACCGGCGCATCCTTATGGGCAGCACGCTG
  AGGAAACGCAAGATGTACGAGGAGTTCCTCAGCAAGGTCTCCATCCTAGAGTCCCTGGAG
  AAGTGGGAGCGTCTGACCGTGGCGGATGCGCTGGAGCCCGTCCAGTTTGAAGATGGAGAG
  AAAATTGTGGTCCAGGGAGAGCCTGGGGACGACTTTTACATCATCACGGAGGGCACCGCG
  TCCGTGCTGCAGCGCCGGTCCCCCAATGAGGAGTACGTGGAGGTGGGGCGCCTGGGACCC
  TCTGACTACTTCGGGGAGATTGCACTGCTGCTGAACCGGCCCCGGGCGGCCACTGTCGTG
  GCCCGGGGGCCCCTCAAGTGTGTGAAGCTGGACCGGCCCCGCTTCGAGCGTGTGCTGGGG
  CCCTGCTCTGAGATCCTCAAGAGGAACATTCAGCGTTACAACAGCTTCATCTCCCTCACC
  GTCTGA
  
  Clone variation with respect to NM_002735.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002735 unedited
  NNNCCTTTTTACCCCGCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTA
  TATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGC
  GGCCGCGAATTCGGCACGAGGGCCATGGCCTCCCCGCCCGCCTGCCCCTCGGAGGAGGAC
  GAGAGCCTGAAGGGCTGTGAGCTGTACGTGCAGCTGCACGGGATCCAGCAGGTCCTCAAA
  GACTGTATCGTCCACCTCTGCATCTCCAAGCCCGAACGCCCCATGAAGTTCCTCCGGGAG
  CACTTCGAGAAGCTGGAGAAGGAAGAAAACAGGCAGATTTTGGCGCGGCAAAAGTCAAAC
  TCACAGTCGGACTCCCATGATGAGGAGGTGTCGCCCACCCCCCCGAACCCTGTGGTGAAG
  GCCCGCCGCCGGCGAGGAGGCGTGAGTGCCGAGGTGTACACCGAGGAGGACGCCGTGTCC
  TACGTCAGGAAGGTGATTCCCAAGGACTACAAAACCATGACTGCGCTGGCCAAGGCCATC
  TCCAAGAACGTGCTCTTCGCTCACCTGGATGACAACGAGAGGAGTGACATATTCGATGCC
  ATGTTCCCTGTCACTCACATCGCTGGGGAGACTGTTATACAGCAAGGGAATGAAGGAGAC
  AACTTCTATGTCGTTGATCAAGGGGAAGTGGATGTGTACGTGAACGGAGAGTGGGTGACC
  AACATCAGCGAGGGAGGCAGCTNTCGNGAGCTGGCGCTCATCTACCGCACCCTCAGGGCT
  GCGACCGTGAAAGCCAAGACGGACCTCCAGCTCTGGGGGATCGACCTGGACAGCTACCTG
  CGCATCCTTATGGGCAGCACGCTGANGCAACGCAGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002735
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002735.1, NP_002726.1
• RefSeq Size: 1932 bp
• RefSeq ORF: 1146 bp
• Locus ID: 5575
• UniProt ID: P31321
• Cytogenetics: 7p22.3
• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Insulin signaling pathway
• Gene Summary: The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All of the variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.