HPS1 (NM_182639) Human Untagged Clone

SKU SC124680

HPS1 (untagged)-Human Hermansky-Pudlak syndrome 1 (HPS1), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: HPS1
• Synonyms: BLOC3S1; HPS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124680 sequence for NM_182639 edited (data generated by NextGen Sequencing); ATGAAGTGCGTCTTGGTGGCCACTGAGGGCGCAGAGGTCCTCTTCTACTGGACAGATCAG; GAGTTTGAAGAGAGTCTCCGGCTGAAGTTCGGGCAGTCAGAGAATGAGGAAGAAGAGCTC; CCTGCCCTGGAGGACCAGCTCAGCACCCTCCTAGCCCCGGTCATCATCTCCTCCATGACG; ATGCTGGAGAAGCTCTCGGACACCTACACCTGCTTCTCCACGGAAAATGGCAACTTCCTG; TATGTCCTTCACCTGTTTGGAGAATGCCTGTTCATTGCCATCAATGGTGACCACACCGAG; AGCGAGGGGGACCTGCGGCGGAAGCTGTATGTGCTCAAGTACCTGTTTGAAGTGCACTTT; GGGCTGGTGACTGTGGACGGTCATCTTATCCGAAAGGAGCTGCGGCCCCCAGACCTGGCG; CAGCGTGTCCAGCTGTGGGAGCACTTCCAGAGCCTGCTGTGGACCTACAGCCGCCTGCGG; GAGCAGGAGCAGTGCTTCGCCGTGGAGGCCCTGGAGCGACTGATTCACCCCCAGCTCTGT; GAGCTGTGCATAGAGGCGCTGGAGCGGCACGTCATCCAGGCTGTCAACACCAGCCCCGAG; CGGGGAGGCGAGGAGGCCCTGCATGCCTTCCTGCTCGTGCACTCCAAGCTGCTGGCATTC; TACTCTAGCCACAGTGCCAGCTCCCTGCGCCCGGCCGACCTGCTTGCCCTCATCCTCCTG; GTTCAGGACCTCTACCCCAGCGAGAGCACAGCAGAGGACGACATTCAGCCTTCCCCGCGG; AGGGCCCGGAGCAGCCAGAACATCCCCGTGCAGCAGGCCTGGAGCCCTCACTCCACGGGC; CCAACTGGGGGGAGCTCTGCAGAGACGGAGACAGACAGCTTCTCCCTCCCTGAGGAGTAC; TTCACACCAGCTCCTTCCCCTGGCGATCAGAGCTCAGGTGAGGACCGGAGGAAAGCAGGA; GGAAACAATAGCTAG; ; Clone variation with respect to NM_182639.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182639 unedited; TTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCGGGCTGTCCCC; TCTGGGGGCGGCGATCCTCCCTCCGGAGCCCCCCTTCAACCCTCCCGGAAGTGAGCATTT; GCAGGACCAGGGATGCTGTGCTGCTCTCCCATGAGCCAGTCACCGAGTCGGTCTGCTGCA; GCCCTTTCTGAACCTCTGGCCGTCTGGATGCTCCACTGTGCTTGCCAAGATGAAGTGCGT; CTTGGTGGCCACTGAGGGCGCAGAGGTCCTCTTCTACTGGACAGATCAGGAGTTTGAAGA; GAGTCTCCGGCTGAAGTTCGGGCAGTCAGAGAATGAGGAAGAAGAGCTCCCTGCCCTGGA; GGACCAGCTCAGCACCCTCCTAGCCCCGGTCATCATCTCCTCCATGACGATGCTGGAGAA; GCTCTCGGACACCTACACCTGCTTCTCCACGGAAAATGGCAACTTCCTGTATGTCCTTCA; CCTGTTTGGAGAATGCCTGTTCATTGCCATCAATGGTGACCACACCGAGAGCGAGGGGGA; CCTGCGGCGGAAGCTGTATGTGCTCAAGTACCTGTTTGAAGTGCACTTTGGGCTGGTGAC; TGTGGACGGTCATCTTATCCGAAAGGAGCTGCGGCCCCCAGACCTGGCGCAGCGTGTCCA; GCTGTGGGAGCACTTCCAGAGCCTGCTGTGGACCTACAGCCGCCTGCGGGAGCAGGAGCA; GTGCTTCGCCGTGGAGGCCCTGGAGCGACTGATTCACCCCCAGCTCTGTGAGCTGTGCAT; AGAGGCGCTGGAGCGGCACGTCATNCCAGCTGTCAACACAGCCCCGAGCGGGAGGCGAGG; GAGCCTGCATGCCTCCTGCTCGTGCACTCAGCTGCTGCATCTACTTAGCCCANGCCAGCT; CCTGCGCCGGCGACTN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_182639 unedited; CCGTCAGGGCCGAGAAAAACCTTTTTTTTTTTGAAATTTATAGTTTTTTTTTATTGTTTA; CCCACATACTCTTGGAGCAAACAAAGTATAAACTACAGATGCTGAATATATTTTACAATG; TATCGAAATCAGATGATTATTACTAAATTGAACTTAATTTTTAATTTTAATTACAAAAAA; GATGAGTCCTGAGAATGCATGTTTTTTTAAGTTTTAAATGAACCAACTTGGCATCAACAG; CTTTAGGGATCAGTGGCGTGGTCTTAACAGCCCTTGACCTCAGGCTGGACCTGGCAGGGA; AGATGGGGAGCCGAAAACAGTGTCCTGTGCTCTAGGAACACGGGCACCTGCACTTAAGCC; TTACGAGGCACGGGGGCCCACTGGAGCCTAACACAGATGCACTGGGCTGCCTGTGGCTCG; TATTAGCTATTGCTGCCCCCTGCTCTACTACGGAGCTAACCTGAGCTCTGACCGCCAGGG; GAAGGAGCTGGCGTCAACTACTTCTTATGGAGGGAGAAGCTGGTTGCCCACTGCAATGGA; CAAGGCGGTTCACGCTCACAGGCTATAACACATGGATCAACCCTGTTCCTGCTCCCCCCA; GCAGGGATGCGAAAGGCCTGATCACCTGCCCAGGATCGTCCATTCCCGTTTCTGACTCCC; CCAAGTGCGCCAGACACCCCGGCTCTCGCAAAGTCACTACCACTGCGTTTCGTGGAGCGA; CGGCACTACGCCTTGTTGCCCGGGCATGCACTGGGTGGTAGTGGCCTTTCCGTTGTCAAG; GCTGCACGCCGAACAATTCTGGATAACAACTGGGACAAAGGCCCTCGACCCTGCGAGATA; CGGATACCGCGGCCTCTTCGACTCCAGGAGCTGTACTGGTGGTTAATCCCACGCATTCAT; TCGGGAGCCACACCCGCAGGTTCGCGCGGTTCGTATATCCACCAGCCGGCTNGCCCTAAA; ACTCGCCGACCGCCCGTTTCCCCCATCTATATCAGACACAAGCGCGGGGGAATTCGGCTT; CCGCACGCCCGTCAATATTN;
• Restriction Sites: NotI-NotI
• ACCN: NM_182639
• Insert Size: 1790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_182639.1, NP_872577.1
• RefSeq Size: 1625 bp
• RefSeq ORF: 975 bp
• Locus ID: 3257
• UniProt ID: Q92902
• Cytogenetics: 10q24.2
• Summary: This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. The encoded protein is a component of three different protein complexes termed biogenesis of lysosome-related organelles complex (BLOC)-3, BLOC4, and BLOC5. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 1. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on chromosome 22. [provided by RefSeq, Aug 2015]; Transcript Variant: This variant (3) differs in the 5' UTR and contains an alternate 3' exon structure compared to variant 1. It encodes isoform c, which has a shorter and distinct C-terminus compared to isoform a.