CPNE1 (NM_152926) Human Untagged Clone

SKU SC124579

CPNE1 (untagged)-Human copine I (CPNE1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: CPNE1
• Synonyms: COPN1; CPN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_152926, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCCACTGCGTGACCTTGGTTCAGCTGTCCATTTCCTGTGACCATCTCATTGACAAGGACATCGGCT; CCAAGTCTGACCCACTCTGCGTCCTTTTACAGGATGTGGGAGGGGGCAGCTGGGCTGAGCTTGGCCGGAC; TGAACGGGTGCGGAACTGCTCAAGCCCTGAGTTCTCCAAGACTCTACAGCTTGAGTACCGCTTTGAGACA; GTCCAGAAGCTACGCTTTGGAATCTATGACATAGACAACAAGACGCCAGAGCTGAGGGATGATGACTTCC; TAGGGGGTGCTGAGTGTTCCCTAGGACAGATTGTGTCCAGCCAGGTACTGACTCTCCCCTTGATGCTGAA; GCCTGGAAAACCTGCTGGGCGGGGGACCATCACGGTCTCAGCTCAGGAATTAAAGGACAATCGTGTAGTA; ACCATGGAGGTAGAGGCCAGAAACCTAGATAAGAAGGACTTCCTGGGAAAATCAGATCCATTTCTGGAGT; TCTTCCGCCAGGGTGATGGGAAATGGCACCTGGTGTACAGATCTGAGGTCATCAAGAACAACCTGAACCC; TACATGGAAGCGTTTCTCAGTCCCCGTTCAGCATTTCTGTGGTGGGAACCCCAGCACACCCATCCAGGTG; CAATGCTCCGATTATGACAGTGACGGGTCACATGATCTCATCGGTACCTTCCACACCAGCTTGGCCCAGC; TGCAGGCAGTCCCGGCTGAGTTTGAATGCATCCACCCTGAGAAGCAGCAGAAAAAGAAAAGCTACAAGAA; CTCTGGAACTATCCGTGTCAAGATTTGTCGGGTAGAAACAGAGTACTCCTTTCTGGACTATGTGATGGGA; GGCTGTCAGATCAACTTCACTGTGGGCGTGGACTTCACTGGCTCCAATGGAGACCCCTCCTCACCTGACT; CCCTACACTACCTGAGTCCAACAGGGGTCAATGAGTACCTGATGGCACTGTGGAGTGTGGGCAGCGTGGT; TCAGGACTATGACTCAGACAAGCTGTTCCCTGCATTTGGATTTGGGGCCCAGGTTCCCCCTGACTGGCAG; GTCTCGCATGAATTTGCCTTGAATTTCAACCCCAGTAACCCCTACTGTGCAGGCATCCAGGGCATTGTGG; ATGCCTACCGCCAAGCCCTGCCCCAAGTTCGCCTCTATGGCCCTACCAACTTTGCACCCATCATCAACCA; TGTGGCCAGGTTTGCAGCCCAGGCTGCACATCAGGGGACTGCCTCGCAATACTTCATGCTGTTGCTGCTG; ACTGATGGTGCTGTGACGGATGTGGAAGCCACACGTGAGGCTGTGGTGCGTGCCTCGAACCTGCCCATGT; CAGTGATCATTGTGGGTGTGGGTGGTGCTGACTTTGAGGCCATGGAGCAGCTGGACGCTGATGGTGGACC; CCTGCATACACGTTCTGGGCAGGCTGCTGCCCGCGACATTGTGCAGTTTGTACCCTACCGCCGGTTCCAG; AATGCCCCTCGGGAGGCATTGGCACAGACCGTGCTCGCAGAAGTGCCCACACAACTGGTCTCATACTTCA; GGGCCCAGGGTTGGGCCCCGCTCAAGCCACTTCCACCCTCAGCCAAGGATCCTGCACAGGCCCCCCAGGC; CTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152926 unedited; NCCGGTTCAAATTTTGNTATACGAACTCACTATAGGGCGGCCGCGAATTCGCACCAGGCT; GCGTTGGGCTCCGGGAAGCCGTTCGGGCTGGGGCTGTCGGCCGCGGGGCGGAGGCACTCG; CGCGGGGGGTAATTCGGGGTCTGGGTTCTGGTGCCGCGCAGCTTTCCCCGATGGCCCACT; GCGTGACCTTGGTTCAGCTGTCCATTTCCTGTGACCATCTCATTGACAAGGACATCGGCT; CCAAGTCTGACCCACTCTGCGTCCTTTTACAGGATGTGGGAGGGGGCAGCTGGGCTGAGC; TTGGCCGGACTGAACGGGTGCGGAACTGCTCAAGCCCTGAGTTCTCCAAGACTCTACAGC; TTGAGTACCGCTTTGAGACAGTCCAGAAGCTACGCTTTGGAATCTATGACATAGACAACA; AGACGCCAGAGCTGAGGGATGATGACTTCCTAGGGGGTGCTGAGTGTTCCCTAGGACAGA; TTGTGTCCAGCCAGGTACTGACTCTCCCCTTGATGCTGAAGCCTGGAAAACCTGCTGGGC; GGGGGACCATCACGGTCTCAGCTCAGGAATTAAAGGACAATCGTGTAGTAACCATGGAGG; TAGAGGCCAGAAACCTAGATAAGAAGGACTTCCTGGGAAAATCAGATCCATNTTCTGGAG; TTCTTCCGCCAGGGTGATGGGGAAATGGCACCTGGTGTACAGATNCTGAGGTCATCAAGA; ACAACCTGAACCCTACTGGAAGCGTTTCTCAGTCCCCGTTCACNATTCTGTTGGGTGGGA; ACCCCAGCACACCCATCCAGGGTGCATGCTCCGATATGACGNGNACGGGTCACATGATCT; CATCGGTACCTTCCCACCAGCTTGGCCAGCTGCAGCAGCCNGNNTTGAAATTTGATGCAT; NCACCTGAGAGCAGCGAAAAGAAAGCTCAGAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_152926
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152926.1, NP_690903.1
• RefSeq Size: 1954 bp
• RefSeq ORF: 1614 bp
• Locus ID: 8904
• UniProt ID: Q99829
• Cytogenetics: 20q11.22
• Summary: Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Alternate splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Aug 2008]; Transcript Variant: This variant (2) uses a different splice site which creates a shorter 5' UTR, compared to variant 1.