NAT1 (NM_000662) Human Untagged Clone

SKU SC124532

NAT1 (untagged)-Human N-acetyltransferase 1 (arylamine N-acetyltransferase) (NAT1), transcript variant 5

Product Data

• Type: Human Untagged Clone
• Target Symbol: NAT1
• Synonyms: AAC1; MNAT; NAT-1; NATI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124532 sequence for NM_000662 edited (data generated by NextGen Sequencing); ATGGACATTGAAGCATATCTTGAAAGAATTGGCTATAAGAAGTCTAGGAACAAATTGGAC; TTGGAAACATTAACTGACATTCTTCAACACCAGATCCGAGCTGTTCCCTTTGAGAACCTT; AACATCCATTGTGGGGATGCCATGGACTTAGGCTTAGAGGCCATTTTTGATCAAGTTGTG; AGAAGAAATCGGGGTGGATGGTGTCTCCAGGTCAATCATCTTCTGTACTGGGCTCTGACC; ACTATTGGTTTTGAGACCACGATGTTGGGAGGGTATGTTTACAGCACTCCAGCCAAAAAA; TACAGCACTGGCATGATTCACCTTCTCCTGCAGGTGACCATTGATGGCAGGAACTACATT; GTCGATGCTGGGTTTGGACGCTCATACCAGATGTGGCAGCCTCTGGAGTTAATTTCTGGG; AAGGATCAGCCTCAGGTGCCTTGTGTCTTCCGTTTGACGGAAGAGAATGGATTCTGGTAT; CTAGACCAAATCAGAAGGGAACAGTACATTCCAAATGAAGAATTTCTTCATTCTGATCTC; CTAGAAGACAGCAAATACCGAAAAATCTACTCCTTTACTCTTAAGCCTCGAACAATTGAA; GATTTTGAGTCTATGAATACATACCTGCAGACATCTCCATCATCTGTGTTTACTAGTAAA; TCATTTTGTTCCTTGCAGACCCCAGATGGGGTTCACTGTTTGGTGGGCTTCACCCTCACC; CATAGGAGATTCAATTATAAGGACAATACAGATCTAATAGAGTTCAAGACTCTGAGTGAG; GAAGAAATAGAAAAAGTGCTGAAAAATATATTTAATATTTCCTTGCAGAGAAAGCTTGTG; CCCAAACATGGTGATAGATTTTTTACTATTTAG; ; Clone variation with respect to NM_000662.5;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000662 unedited; TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATTCAGTCTAGTTCC; TGGTTGCCGGCTGAAATAACCTGAATTCAAGCCAGGAAGAAGCAGCAATCTGTCTTCTGG; ATTAAAACTGAAGATCAACCTACTTTCAACTTACTAAGAAAGGGGATCATGGACATTGAA; GCATATCTTGAAAGAATTGGCTATAAGAAGTCTAGGAACAAATTGGACTTGGAAACATTA; ACTGACATTCTTCAACACCAGATCCGAGCTGTTCCCTTTGAGAACCTTAACATCCATTGT; GGGGATGCCATGGACTTAGGCTTAGAGGCCATTTTTGATCAAGTTGTGAGAAGAAATCGG; GGTGGATGGTGTCTCCAGGTCAATCATCTTCTGTACTGGGCTCTGACCACTATTGGTTTT; GAGACCACGATGTTGGGAGGGTATGTTTACAGCACTCCAGCCAAAAAATACAGCACTGGC; ATGATTCACCTTCTCCTGCAGGTGACCATTGATGGCAGGAACTACATTGTCGATGCTGGG; TTTGGACGCTCATACCAGATGTGGCAGCCTCTGGAGTTAATTTCTGGGAAGGATCAGCCT; CAGGTGCCTTGTGTCTTCCGTTTGACGGAAGAGAATGGATTCTGGTATCTAGACCAAATC; AGAAGGGAACAGTACATTCCANATGAAGAATTTCTTCATTCTGATCTCCTAGAAGACAGC; AAATACCGAANAATCTACTCCTTTACTCTTNAGCCTCGAAACAATGAAAGATTTTGAGTC; TATGAATACATACCTGCAGACATCTCCATCATCTGGGGTTACTAGNAAATCATTNTGTTC; CTTGCAGACCCCAGATGGGNTCACTGGTTGGTGGGCTTCCCCTCCCCCCATAGAGATCAT; ATAGGACATACGATTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_000662
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000662.4, NP_000653.3
• RefSeq Size: 1438 bp
• RefSeq ORF: 873 bp
• Locus ID: 9
• UniProt ID: P18440
• Cytogenetics: 8p22
• Domains: Acetyltransf2
• Protein Pathways: Caffeine metabolism, Drug metabolism - other enzymes, Metabolic pathways
• Summary: This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human genome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]; Transcript Variant: This variant (5, also known as Type IIA) lacks two alternate exons in the 5' UTR, compared to variant 1, and is the predominant transcript. This variant is transcribed from a promoter known as P1, promoter 2, or NATb promoter. Variants 1-6 and 9 all encode the same isoform (a).